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A strain of Geobacter geo-lyy for dehalogenation of halogenated organic matter and decolorization of dyes and its application

A geobacteria and strain technology, applied in the field of microbiology, can solve the problem of insignificant dehalogenation effect, achieve strong environmental adaptability and growth ability, enhance in-situ microbial restoration ability, and improve the effect of groundwater quality and environment

Active Publication Date: 2022-05-10
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defects and deficiencies that the dehalogenation effect of the existing halogenated organic substances is not significant, to use Geobacter to carry out the environmental condition tolerance of halogenated organic substances in the application of environmental remediation, and to explore the method of compound pollution remediation The method provides a theoretical basis, and provides a Geobacter Geo-LYY strain for dehalogenation of halogenated organic matter and decolorization of dyes and its application

Method used

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  • A strain of Geobacter geo-lyy for dehalogenation of halogenated organic matter and decolorization of dyes and its application
  • A strain of Geobacter geo-lyy for dehalogenation of halogenated organic matter and decolorization of dyes and its application
  • A strain of Geobacter geo-lyy for dehalogenation of halogenated organic matter and decolorization of dyes and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The acquisition of embodiment 1 ground bacterium (Geobacter lovleyi) LYY

[0034] 1) Isolation and cultivation of strains

[0035] 1. Experimental method

[0036] Sediment samples were collected from the black and odorous water body in Tangxia City, Guangzhou City, Guangdong Province, and stored at 4°C. After the sediment samples were stirred evenly, 5 mL was inoculated into the anaerobic dechlorination liquid medium (the formulation of which was PCE as the only electron acceptor) As shown in Table 1), cultured at 30°C while monitoring the dechlorination of PCE; after three generations of enrichment culture, a high-efficiency bacterial group with complete dechlorination ability to PCE was obtained; by controlling the passage time, the enrichment After collecting, adding antibiotics to kill miscellaneous bacteria and continuous gradient dilution and purification operations, a purified bacterial strain was obtained, and the purified bacterial strain was named strain LYY....

Embodiment 2

[0061] Example 2 Tolerance of Geobacter LYY under PCE substrate concentration pressure and environmental condition pressure

[0062] 1. Experimental method

[0063] Geobacter LYY was inoculated in the PCE dechlorinated culture solution, and the substrate (PCE) concentration pressure and environmental conditions (antibiotics, heavy metal Cu 2+ and Cd 2+ ) Tolerance determination under pressure, concrete experimental method and experimental result are as follows:

[0064] (1) Geobacter LYY tolerance PCE concentration experiment: Under the conditions of acetate concentration of 10mM, Geobacter LYY inoculation amount of 2.5%, and culture temperature of 30°C, the PCE concentration was set to 0.25mM, 0.5mM, 1mM, 2mM , 3mM, 5mM, to investigate the dechlorination of Geobacter LYY to PCE.

[0065] (2) Antibiotic resistance experiment of Geobacter LYY: When the concentration of acetate is 10 mM, the concentration of PCE is 1 mM, the inoculum amount of Geobacter LYY is 2.5%, and the c...

Embodiment 3

[0071] The mensuration of embodiment 3 Geobacter LYY dye decolorization ability

[0072] Geobacter LYY was inoculated in the anaerobic medium (its formula is shown in Table 3) with azo dye as the only electron donor without reducing agent, and the decolorization ability of live bacteria of azo dye was tested. The specific experimental method And the experimental results are as follows:

[0073] 1. Experimental method

[0074] Under the conditions of 10mM acetic acid concentration, 2.5% Geobacter LYY inoculum, and 30°C culture temperature, 60ppm of azo dye AO7 was set as the only electron acceptor, and a blank sample not inoculated with the strain was used as a reference under the same conditions. , the standard curve was measured by spectrophotometry, and the decolorization of the azo dye AO7 by Geobacter LYY was determined.

[0075] Table 3 does not contain the anaerobic liquid medium formula of reducing agent

[0076]

[0077]

[0078] 2. Experimental results

[00...

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Abstract

The invention discloses a Geobacter Geo-LYY strain with halogenated organic matter dehalogenation and dye decolorization and its application. The present invention screened and isolated a strain of Geobacter LYY with the ability to dehalogenate halogenated organics and decolorize dyes at the same time. : 61003. The Geobacter LYY can tolerate high concentrations of perchloroethylene, antibiotics, heavy metal Cu 2+ and heavy metal Cd 2+ The pressure of environmental conditions, and maintain the high-efficiency dechlorination effect of tetrachloroethylene, and the Geobacter LYY can achieve the effect of complete decolorization of the azo dye AO7; It has good application prospects in the halogen bacteria agent, as well as in the decolorization of dyes or the preparation of dye decolorizers.

Description

technical field [0001] The invention belongs to the technical field of microbiology. More specifically, it relates to a Geobacter Geo-LYY strain for dehalogenating organic matter and decolorizing dyes and its application. Background technique [0002] As a commonly used organic solvent, perchlorethylene (PCE) is widely used as metal degreasing detergent, dry cleaning agent, general solvent and organic synthesis intermediate. Due to the volatile nature of tetrachlorethylene and improper handling during production, storage and use, a large amount of tetrachlorethylene enters the environment and pollutes the atmosphere, surface water, soil and groundwater. Perchlorethylene will enter the soil due to dumping, leakage, or rainwater runoff, and part of it will even leak into groundwater, resulting in groundwater pollution. Pollution of tetrachlorethylene and trichlorethylene in groundwater is especially common; at the same time Perchlorethylene has strong environmental toxicity ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/36C02F101/38
CPCC12N1/20C02F3/34C02F2101/308C02F2101/36C02F2101/38C12R2001/01C12N1/205
Inventor 汪善全梁咏颐吴日枫
Owner SUN YAT SEN UNIV
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