133results about How to "Improve growth ability" patented technology

The invention relates to a lactobacillus plantarum strain of which the preservation number is CGMCC No.3166, which can be enriched in a modified MRS liquid culture medium. The lactobacillus plantarum strain is a white colony on an MRS solid culture medium, is straight, short-chained, Gram-positive and atrichous, has homofermentation, does not generate gas when fermenting glucose, generates acetic acid and lactic acid when fermenting ribose, and does not reduce nitrite. In application, the strain is activated and propagated and thalli are collected to form a fermenting agent which can inhibit the growth of amine producing bacteria in the fermenting production of meat products and generate biogenic amine oxidase which oxidizes and degrades tyramine, phenylethylamine, tryptamine and histamine. The invention has the advantages that the lactobacillus plantarum has strong growing ability, the fermenting agent has simple preparation technology and can effectively inhibit the growth and propagation of enterobacteria in the fermenting production of meat products and control the generation of biogenic amine, the generated biogenic amine oxidase can oxidize and degrade the contents of tyramine, phenylethylamine, tryptamine and histamine, and the lactobacillus plantarum strain as the fermenting agent for producing the fermented meat products can effectively reduce the accumulation of the biogenic amine.

The invention discloses bioprotein sponge and a preparation method thereof. According to the characteristics that internal cells of a human body and an animal body adapt to cell growth environments, the bioprotein is freezed into a dry sponge shape through freeze drying technology by utilizing intercellular substances as controlled-release carrieres of growth factors and acts on the trauma of the human body or the animal body in controlled-release medicament feeding mode. The preparation method of the bioprotein sponge changes the traditional preparation process, adopts S / D virus inactivation, and adds protective agent to reduce probability of virus cross infection of people and livestock simultaneously. The CM cation chromatographic purification process improves purity of bioprotein, promotes stanching and tissue union effect of the bioprotein sponge and reduces clinical side effect. An ultrafiltration process is adopted to replace a dialysis process, so that preparation period is shortened, activity of the bioprotein is protected, and production cost is reduced. A method of heating inactivation virus replaces the method of cobalt60 radiation sterilization, so that the fact that radiation rays bring damage to products is avoided, the activity of the bioprotein is protected, and safe and reliable use of products in clinical application is achieved.

The invention relates to a knitted multilayered-structure nanofiber yarn tendon scaffold as well as preparation and application thereof. Yarn is of a multilayered core spun structure. The preparationcomprises steps as follows: core layer yarn penetrates through a hollow rotating funnel to serve as a receiving device, positive and negative high-voltage power sources are applied to double needles on the two sides respectively, electrospun nanofibers are twisted to the core layer yarn, and double-layer continuous nanofiber yarn is prepared; the double-layer nanofiber yarn is taken as a core layer, electrospun natural polymer nanofibers are arranged on the outer layer, and three layers of nanofiber yarn are prepared; finally, the 3D knitted scaffold is prepared from the different layers of nanofiber yarn with a knitting technology and is used for tendon repair. The knitted multilayered-structure nanofiber yarn scaffold has the characteristics that a function of enhancing mechanical properties of regenerated tissue for a long time is realized, biocompatibility is good and repair and normal function recovery of tendon injury are facilitated.

The invention discloses uses of a marine yeast, preservation No. of the marine yeast Rhodosporidimu paludigenum Fell & Tallman is IMI 394084, the marine yeast can be used in microorganism fuel cell for electricity generation directly. The invention discloses a microorganism fuel cell at the same time, including an open anode chamber (2) with an anode electrode (5) provided inside and an open cathode chamber (1) with a cathode electrode provided inside (4), a proton exchange membrane is provided between the anode chamber (2) and the cathode chamber (1), a liquid organic fuel containing bacteria solution is provided in the anode chamber (2), and the bateria solution is obtained by fermenting the marine yeast of preservation No. 394084. The invention also discloses method of preparing the microorganism fuel cell at the same time. The microorganism fuel cell of the invention is provided with features of environment friendly.

The invention discloses a planting method for combine circular agriculture, which comprises the following steps of planting evergreen first high-bar trees and / or undershrub around cultivated fields of cash crop to form a sand storm protection zone for the cash crop; planting second high-bar trees at the periphery of the wind storm protection zone to form a hedgerow zone of the cash crop; and planting first insect pest prevention plants between the wind storm protection zone and the hedgerow zone to form a first insect pest prevention zone. The method provides appropriate growth environment for the cash crop by planting various trees and undershrub plants to respectively form the wind storm protection zone, the first insect pest prevention zone and the hedgerow zone, thereby preventing the cash crop from being damaged by sand and insect pests, reducing using amount of pesticide and improving quality of agricultural products.

The invention belongs to the field of biotechnology, and particularly relates to pseudomonas hibiscicola capable of degrading tetracycline pollutants in water and application thereof. The pseudomonas hibiscicola DT1 is preserved in China center for type culture collection with the preservation number of CCTCCNO.M2014244. The pseudomonas hibiscicola DT1 has stronger growth capability and good tetracycline degradation capability in the environment containing the tetracycline, the strain can effectively remove the tetracycline in wastewater within 72 hours, and the removal efficiency reaches 83.21wt%. The pseudomonas hibiscicola provides a useful source of bacteria for degradation of tetracycline organic pollutants, the function aspect application of pseudomonas hibiscicola is broadened, and the pseudomonas hibiscicola has strong application value.

The invention discloses a preparing method for repetitive-gradient porous titanium alloy for promoting sacralization. The repetitive-gradient porous titanium alloy takes titanium alloy powders as raw materials, and repetitive-gradient porous titanium alloy bar materials are prepared by 3D printing; elasticity modulus of the gradient porous titanium alloy is at 0.53-13.2 GPa, and the compression and flexion-extension strength is changed at 22-356 Mpa, and the elasticity modulus of the gradient porous titanium alloy is matched with the elasticity modulus of natural bones. The preparing method for the repetitive-gradient porous titanium alloy for promoting sacralization comprises the following steps that hydroxyapatite (HA) coating with bioactivity is constructed on the surface of the repetitive-gradient porous titanium alloy through a plasma spraying technique; and the growth factor (VEGF) is fixed to surfaces of materials by utilizing dopamine as an intermediate medium. The porous titanium alloy materials prepared through the preparing method is loaded with the growth factor and the HA and has the characteristics that histocompatibility is high, and migration and growth of osteocyte can be promoted; and according to the repetitive-gradient porous structure, the fusion strength of bones is increased, and pullout force is increased.

The invention discloses a paecilonyces variotii strain H6 and application thereof. The paecilonyces variotii strain H6 with a preservation number of CGMCC No. 3722 is preserved at China General Microbiological Culture Collection Center on April 9, 2010. The paecilonyces variotii strain H6 widely spreads in the nature, quickly propagates and has strong growth capability. The invention provides the application of the paecilonyces variotii strain H6 in formaldehyde degradation and provides the technical support for the biological treatment engineering with formaldehyde pollution.

The invention discloses a nanometer reinforced seaweed culture medium capable of improving a red kiwi fruit transplantation survival rate. The nanometer reinforced seaweed culture medium comprises the following ingredients in parts by weight: 200-210 parts of seaweed, 50-52 parts of nanometer white carbon black, 8-9 parts of EM (Effective Microorganisms) flora fermentation broth, 23-25 parts of saccharose, 70-72 parts of pyroligneous liquor, 89-90 parts of sawdust, 75-76 parts of peanut shell, 67-68 parts of Chinese medicine residue, 5-6 parts of cellulose, 16-17 parts of EDTA (Ethylene Diamine Tetraacetic Acid) chelating zinc, 12-13 parts of EDTA chelating boron, 11-12 parts of Ageratina adenophora extractive and the like. The seaweed and the ultrafine sawdust, peanut shell and Chinese medicine residue are subjected to enzymolysis, fermentation and nanometer reinforcement to prepare a fruit tree transplantation medium, the energy of a transplanted fruit tree root system can be accelerated, the soil environment of the transplanted fruit tree root system is improved, meanwhile, the adopted Ageratina adenophora extractive and the like have good functions of accelerating repair and growth for the transplanted damaged fruit tree root system, and all the ingredients can be combined to perform the efficacies on improving the red kiwi fruit transplantation survival rate.

The present invention is an electronic circuit experiment board, which is composed of an insulated base plate, copper binding posts and a bracket and so on. The base plate is divided into an upper plate and a lower plate, which have binding post inserting holes and circlips. The binding posts can be inserted into the binding post inserting holes and then be fastened by the circlips. There are a binding hole and a screw hole on the ends of the binding posts respectively. The binding post also includes a screw lever cooperated with the screw hole, a positioning groove and a positioning ring. The experiment board has the advantages of good contact performance, convenient to use, high experimental efficiency, long service life and strong ability of splicing and dilatancy, arranging elements in double sides, and multilayer wiring in electronic circuit.

The invention discloses uses of a marine yeast, preservation No. of the marine yeast Rhodosporidimu paludigenum Fell & Tallman is IMI 394084, the marine yeast can be used in microorganism fuel cell for electricity generation directly. The invention discloses a microorganism fuel cell at the same time, including an open anode chamber (2) with an anode electrode (5) provided inside and an open cathode chamber (1) with a cathode electrode provided inside (4), a proton exchange membrane is provided between the anode chamber (2) and the cathode chamber (1), a liquid organic fuel containing bacteriasolution is provided in the anode chamber (2), and the bateria solution is obtained by fermenting the marine yeast of preservation No. 394084. The invention also discloses method of preparing the microorganism fuel cell at the same time. The microorganism fuel cell of the invention is provided with features of environment friendly.

The invention provides an enriching and screening method for tumor stem cell. The combination of ball culture and immunomagnetic beads separation method is used for enriching and screening the tumor stem cells, the details are as follows: continuously culturing the tumor cells in a no-serum ball culture medium, using the QPCR method to simultaneously examine the relative levels of tumor stem cell markers, when the expression levels of specific marker is relatively the highest, supplement with immunomagnetic and positive, single or double screening tumor stem cells. The combination of the two screening methods can significantly improve such practical problems as low separation rates, low purity levels and the inconveniences of after application for tumor stem cells.

The invention discloses Cladosporiumc cladosporioides for high yield of feruloyl esterase and an application of Cladosporiumc cladosporioides in edible vinegar brewing, and belongs to the field of fermentation engineering and biotechnology. The Cladosporiumc cladosporioides was preserved by the China Center for Type Culture Collection, the preservation number is CCTCC NO: M2015549, and the preservation date is September, 16th, 2015. According to the invention, wheat bran is used as a fermentation culture medium for fermentation production of high-activity feruloyl esterase, and it is easy to realize industrial production of feruloyl esterase. By applying feruloyl esterase to production of edible vinegar, functional health-care edible vinegar rich in ferulic acid is obtained. Thus, new technical support is provided for development of health-care vinegar.

The invention belongs to the technical field of planting and particularly relates to a planting method for lotus roots. The planting method for lotus roots includes the following steps that firstly, a site is selected for building a pond; secondly, the lotus root pond is erected; thirdly, certified seeds are selected; fourthly, transplanting is performed; fifthly, management and fertilization are performed; sixthly, disease and insect damage is prevented. The planting method for lotus roots has the advantages that the growth capacity of lotus roots is improved, the survival rate of lotus roots is increased, planning and construction are reasonable, management can be conveniently performed in the lotus root cultivation process, and the yield of lotus roots is increased.

The invention relates to the field of plant tissue culture and discloses a tissue culture method for increasing the survival rate of psidium guajava L.. The tissue culture method comprises the following first step of disinfection of a culture room, the second step of selection of explants, the third step of sterilization of the explants, the fourth step of initial induction culture, the fifth step of subculture, and the sixth step of seedling hardening. According to the method, the explants are disinfected with plant treating liquid, and thus the survival rate of the explants in the tissue culture process is increased; meanwhile, a commonly-used MS culture medium is replaced with cassava alcohol waste liquid, and thus the tissue culture cost can be effectively reduced. The tissue culture method can be applied to various explants of psidium guajava L., the surfaces of the explants can be effectively disinfected without disoperation to the explants, and meanwhile the production cost can be effectively reduced.

The invention belongs to the field of food, in particular to the technical field of condiment preparation in the food industry, specifically to an aspergillus oryzae strain PCSM002 and application thereof. The aspergillus oryzae strain PCSM002 is named as Aspergillus oryzae with a preservation number of GDMCC No:60198. The aspergillus oryzae further produces protease highly, and further can be used for producing thick broad-bean sauce. The strain is strong in growth ability, is high in starter propagation speed and is strong in protease production ability. The strain can be adopted to quicklyprepare thick broad-beans at a high fermentation speed, is high in amino nitrogen content, is relatively superior in flavor, can improve the quality and the flavor of thick broad-bean sauces, and provides a scientific basis for improving industrial production of thick broad-bean sauces.

The invention provides Aspergillus awamori for producing feruloyl esterase and application thereof. The Aspergillus awamori has the category name of Aspergillus awamori black orange inhibiting strain, is collected by China center for type culture collection, called CCTCC for short, at December 8, 2013, has the collection number of CCTCCNO: M2013639 and is applied to the production of feruloyl esterase. According to the strain provided by the invention, a method that feruloyl esterase is produced through fermenting by taking wheat bran as a fermentation substrate and bound-type phenolic and ferulic acids are obtained through rupturing ester bonds between the polysaccharide and lignin of plant cell walls and ferulic acid by the feruloyl esterase is adopted, and the condition that microorganisms are short in growth cycle, are not restricted to climate and are easily subjected to large-scale production is utilized, so that a novel biological method for extracting bound-type phenolic acid substances, such as ferulic acid is provided.

The invention relates to a production method of a stropharia rugosoannulata strain. The production method disclosed by the invention comprises the following steps of: 1) processing raw materials; 2) blending the materials; 3) bottling; 4) performing high-pressure sterilization; 5) cooling and inoculating; and 6) culturing. The production method disclosed by the invention has the beneficial effects that the quality of the stropharia rugosoannulata strain can be improved, high-quality variety genes can be ensured, the strain is healthy, thick and strong in mycelia, the germination is fast, the growth ability is strong, the probability of mixed bacterium infection is reduced and the success rate of base production of stropharia rugosoannulata is improved by making the stropharia rugosoannulata strain through the steps above.

Provided are a preparation method and application of a zeolite-modified water body cadmium adsorbent. The preparation method comprises the following steps: (1) performing crushing, sieving and grinding on natural clinoptilolite so as to obtain zeolite of a fine powder particle size; (2) performing high temperature and lanthanum chloride modification on the zeolite of the fine powder particle sizeso as to obtain modified zeolite of the fine powder particle size; (3) adopting lignin as a raw material, and preparing nano carbon fiber-coated modified zeolite so as to obtain the zeolite-modified water body cadmium adsorbent. The zeolite-modified water body cadmium adsorbent prepared by using the method is suitable for a cadmium-contaminated natural water body, can be packed in a nylon mesh bagand move in the polluted natural water body, or is filled into an adsorption filler tower or pool, so that the purpose of cadmium removal from wastewater is achieved. The raw materials are cheap andeasily available, and the prepared adsorbent has a wide application range, a good adsorption effect and no secondary pollution, and is especially suitable for emergency treatment of a low-concentration suddenly cadmium-contaminated natural water body or treatment of cadmium-containing wastewater with the concentration of 10-40 mg / L; and the treatment efficiency can reach 95% or above.

The invention relates to a multifunctional compound grouting material. The grouting material is prepared from, by weight, 2640-2760 parts of superfine cement, 50-80 parts of modified nanoscale calcium powder, 17-25 part of polycarboxylate superplasticizer, 60-120 parts of superfine steel slag fine powder, 75-120 parts of superfine high-calcium fly ash, 10-20 parts of superfine fluorine gypsum powder, 0.1-0.3 part of secondary nanoscale polymer and 1-3 parts of aluminum magnesium silicate, and the raw materials are evenly stirred to obtain the material. The multifunctional compound grouting material is reasonable in composition, low in production cost, good in working performance, easy to process, environmentally friendly, energy-saving, pollution-free, and wide in application range.

The invention provides application of a Bacillus cereus DS1 in degradation of organic pollutants in saponin waste water. The Bacillus cereus DS1 is collected at China Center for Type Culture Collection, and the collection number is CCTCC NO:M2010145. The Bacillus cereus DS1 has high growing capability and favorable capability of removing organic pollutants in saponin waste water in the presence of high-concentration sulfate ions. The strain can remove COD (chemical oxygen demand) in saponin waste water by 2550 mg / L within 72 hours, and the removal efficiency is up to 60.14%. The invention provides a useful bacterium source for the degradation of high-concentration organic pollutants in saponin waste water, and widens the functional application of Bacillus cereus, thereby having high application values.

The invention discloses radiation-resistant type paenibacillus sp. KH9 and an application thereof in a biological antitranspirant. A soil sample collected in arid desert region around the Lop Nor area in Xinjiang Province is separated, screened and cultured, and the radiation-resistant type paenibacillus sp. KH9 with relatively high polysaccharide production capacity and the function of reducing plant transpiration is obtained and has the CGMCC No.12799. The radiation-resistant type paenibacillus sp. KH9 has remarkable physiological-biochemical characteristic difference and molecular level difference by comparison with common paenibacillus sp.; when the screened-out radiation-resistant type paenibacillus sp. KH9 and the antitranspirant are compounded and used for drought resistance, biological films are formed on leaves of crops, transpiration of the crops can be reduced effectively, the photosynthesis function is improved, the chlorophyll content is increased by 4.50%, and the radiation-resistant type paenibacillus sp. KH9 have higher growth promoting capacity, osmotic adjustment ability and active oxygen defense capacity for crops and can reduce damage to the crops under drought stress.

The invention relates to a symbiotic bacterium of entomopathogenic nematode and an application of the symbiotic bacterium. The bacterium is xenorhabdus budapestensis HEB02, which is preserved in China General Microbiological Culture Collection Center on No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing on April 11, 2016, with preservation number of CGMCC No.12352. The bacterium is used for controlling soybean soreshin blight. The xenorhabdus budapestensis HEB02 provided by the invention is relatively strong in capacity of inhibiting promycelium growth of the soybean soreshin blight; and as increase in volume concentration, an antibacterial effect becomes more obvious.

The invention discloses a preparation method of a titanium alloy implant biological surface active coating. The method comprises the steps of placing the titanium alloy implant in the electrolyte, taking the titanium alloy implant as an anode and an electrolytic cell as a cathode, controlling the temperature of the electrolyte not to be larger than 40 DEG C, adopting a pulse power source for a micro-arc oxidation process, and forming a micro-arc oxidation coating with biological activity on the surface of the titanium alloy implant. According to the present invention, the porous titanium alloyimplant is prepared through a selective laser melting 3D printing technology, and a coating with biological activity is generated on the surface of the titanium alloy porous implant in situ, so thatthe protein adsorption, cell adsorption and cell proliferation capabilities of the surface of the customized porous implant are remarkably enhanced, and the new bone ingrowth capability of the titanium alloy porous implant after an implantation operation is improved.

The invention discloses a surface treatment method of silicon-doped calcium phosphate ceramics. The surface treatment method is to perform surface hydro-thermal treatment to the silicon-doped calcium phosphate ceramics and comprises the steps of: placing the silicon-doped calcium phosphate ceramics into a sterilization boiler, treating for 0.5-2h at a temperature of 100-180 DEG C, washing by deionized water, drying and thus obtaining the silicon-doped calcium phosphate ceramics with hydroxylapatite crystal whiskers grown on the surface. The surface treatment method provided by the invention is simple in process, and can obtain the hydroxylapatite crystal whiskers which are controllable in size and have the diameters in micron level to nanometer level.

The invention relates to a coxsackie A group 5 type (CV-A5) virus strain capable of effectively infecting adult young mice. The virus strain is CV-A5-M14-611, and has a complete amino acid sequence shown as SEQ ID NO. 1. The invention also relates to an attenuated strain CV-A5-M14-111 of the CV-A5 virus strain, and the attenuated strain has a complete amino acid sequence shown as SEQ ID NO. 3. Theinvention also relates to application of the virus strain in preparation of an animal model for evaluating vaccines and / or medicines for treating hand, foot and mouth disease (HFMD) and application of the virus strain in preparation of a preparation for evaluating a protection effect of the vaccines for treating the HFMD.

The invention discloses a silage fermentation accelerator. The accelerator is prepared by mixing pediococcus pentosaceus bacterial liquid having an effective viable count of more than or equal to 10<11>CFU / mL and bacillus subtilis bacterial liquid having an effective viable count of more than or equal to 10<10> CFU / mL in a volume ratio of (1-2):(1-1.5). The silage fermentation accelerator is applied to corn straw coating, bundling and silage fermentation, a method of spraying and adding bacterial liquid is adopted, and the dosage of the bacterial liquid is 4L / ton. According to the method, corn straws are rubbed by utilizing a straw rubbing machine, are spread and aired until corn straws contain proper moisture, are sprayed with the silage bacterial liquid, are uniformly mixed, are coated and bundled by means of a coating machine, are fermented for about 30 days and are unpacked for use. The production method is simple and feasible, and has the advantages of high efficiency, strong practicality and high economic value.

The invention discloses a planting and breeding combination ecological circulation method for a saccharum officinarum field, and belongs to the ecological agriculture field. The planting and breedingcombination ecological circulation method comprises the following steps of: (1) seed selection; (2) soil preparation and fertilizer application; (3) seeding conduction; (4) field management: a: carrying out reasonable fertilizer application, b: carrying out moisture management and c: thinning; (5) aquaculture: 1) carrying out drainage culture, and 2) carrying out moisture and food management; and(6) harvesting conduction. The planting and breeding combination ecological circulation method disclosed by the invention fully performs the advantages of the stereoscopic agriculture of the saccharumofficinarum field, a stereoscopic planting and breeding mode of the saccharum officinarum field is adopted, Cucumis sativus L. is planted among different pieces of saccharum officinarum, and fishes and Cipangopaludina chinensis Gray are cultured in grooves among furrows. Compared with a traditional saccharum officinarum cultivation way, the planting and breeding combination ecological circulationmethod has a better spatio-temporal allocation among species, and a colony ecological factor is further optimized.

The bamboo tea beverage is prepared with natural bamboo juice, bamboo leaf and tea as basic material, and may be in the form of tea, granule or canned liquid beverage. It has intense bamboo scent and tea scent, smooth oral feeling, rich nutrients, and health functions of clearing away heat, diminishing inflammation, promoting digestion, etc.