The application belongs to the field of biological agents, and particularly relates to a high-efficiency
trapping method of
insect pathogenic nematodes, which adopts Galleria mellonella treated by
immune barrier weakening and
cocooning inhibition in sequence for
trapping and collecting; and specifically comprises the following steps: S1, collecting fresh soil samples from the wild; S2, inoculating biocontrol
bacteria into the soil samples, spraying pheromones and moisturizers, and mixing; S3, placing the Galleria mellonella treated in the above step into a
trapping agent ball, and filling the soil samples treated in the step S2 into the trapping agent ball and compacting; S4, burying the trapping agent ball in the remaining soil samples, and after completion, placing the soil samples in a sample bag in a
thermostat with sufficient
oxygen for trapping, the application can improve the success rate of
nematode infection, is beneficial to further propagation, collection and application of
insect pathogenic
nematode resources, and has the advantages of simple method and simple operation.