This invention relates to method,
in vivo and
in vitro for
mass production of entomopathogenic nematodes as a biological control agent for laboratory use and small scale field application against
agricultural pest.
In vivo production has managed to sustain as a cottage industry.
In vivo production is likely to continue as small business venture for niche markets. For
in vivo production eight Pakistani strains of entomopathogenic nematodes were
mass cultured using G. mellaonella larvae at different concentrations and
in vitro production were carried out in soya and
wheat flour medium. Average
in vivo production potential at were 14 x 106 infective juveniles and
in vitro production potential ranged up to 50* 10 6 inefective juveniles.