104 results about "Entomopathogenic nematode" patented technology

Herein is disclosed an apparatus for intercepting and interdicting target organisms such as subterranean termites, which apparatus is comprised of an outer shell fitted with a port for organism ingress and egress and a dorsal cover fitted with a signal port for visual inspections. Inside are materials suitable as food for the organisms targeted by the device, so arranged and comprised as to aid and encourage the habitation, propagation, and retention of biological pesticides, including entomopathogenic nematodes. The subject invention teaches a method of gradually deploying its apparatus to intercept the presence, measure the strength and vigor, and interdict aggregations, of said target organisms. The method permits minimal, systematic deployments that undergo progressive evolutions as information from each deployed device accumulates. Reliance on simple, accurate, visual signals, to determine servicing and supplementation requirements, reduces the user's inspection and servicing time, minimizes the use of interdiction agents, and speeds the interdiction of the targeted organism at the site.

The invention relates to a pollution-free method of combining entomopathogenic nematode and black sticky boards for controlling bradysia odoriphaga. During bradysia odoriphaga larval stage, a entomopathogenic nematode suspension liquid with a nematode concentration of 500-1000IJS/mL is applied, and the black sticky boards are suspended in the field for monitoring. When adult bradysia odoriphaga appears, a large number of black sticky boards are suspended, wherein the lower bottom edges of the boards are 0.5-1cm from the ground. 60-80 of the black sticky boards are suspended in each mu of field. According to the controlling method provided by the invention, biological control and physical control are combined. The colored boards are used for trapping and killing adult bradysia odoriphaga, such that pest cardinal number can be effectively reduced. Bradysia odoriphaga larva and adult controlling effects can be improved, and entomopathogenic nematode application amount can be reduced. Therefore, pollution-free, safe, and non-toxic controlling over bradysia odoriphaga pests can be realized.

The invention discloses a method for breeding entomopathogenic nematodes. The method is realized in a way that: a transparent culture container is used as a breeding container, a live yellow mealworm is used as a host insect, phytodetritus is used as a culture medium, a sterilizing device is used for sterilization, and a temperature-control culture room is used for culturing. The invention obviously solves the problem of waste pollution of plant straws and wood, greatly reduces the production cost for the raw materials and simplifies the procedures for production and use. The cultured entomopathogenic nematodes have the advantages of low cost, good quality and good effects for prevention and control, and can be widely used for preventing and controlling subterranean pest-insect and concealed insects of plants in farm land, forests, gardens and the like.

The invention provides a preparation method of steinernema carpocapsae. The steinernema carpocapsae belongs to steinernematidae of rhabditida of phasmida, and is one of the entomopathogenic nematodes which are most widely and potentially applied to biological control. Symbiotic bacteria exist in bodies of the entomopathogenic nematodes and enter an insect along with the nematodes to result in blood poisoning which causes the death of the insect; and the steinernema carpocapsae has a unique advantage and a good control effect on control of concealed pests such as borers and soil pests.

The invention pertains to the technical field of forestry production, relating to a high cultivation method of entomopathogenic nematode in a large scale. The invention changes the present propagation in vitro into living propagation, which improves the pathogenic capability to pest in agriculture. The invention has high output and efficiency with low cost and good quality. The control effects are increased remarkably. The material used in the invention is widely distributed and inexpensive, which can reduce cost and be widely applied to the control towards hidden pest in forest, fruit trees, and ornamental gardens etc. and the underground pest in pot plants and cash crops. The invention can further improve the production quantity and quality of entomopathogenic nematode and promote the development of Biological control in our country.

The invention discloses a control method of Spodoptera frugiperda. The control method comprises the following steps: step one, eluting entomopathogenetic nematodes from sponge blocks by using water toprepare entomopathogenetic nematode suspension liquid, wherein the entomopathogenetic nematode suspension liquid consists of water and entomopathogenetic nematodes; step two, uniformly applying the entomopathogenetic nematode suspension liquid obtained in the step one to leaf surfaces of crops and/or soil of the crops; step three, infecting the larvae of Spodoptera frugiperda on the leaf surfacesof crops and/or on the surface of soil by using entomopathogenetic nematodes, and infecting the aged larvae, prepupa and pupae of the Spodoptera frugiperda under the soil of the crops. According to the method, Spodoptera frugiperda is used for infecting leaf surface insects and underground insects of the Spodoptera frugiperda, biological control of the Spodoptera frugiperda is carried out, the environment is not polluted, drug resistance to the Spodoptera frugiperda is not generated, the Spodoptera frugiperda continuously proliferates in the environment, population can be established in the environment, and a controllable and preventable state caused by continuous and stable control of pests is achieved.

The present invention belongs to the field of agricultural production technology, and is especially industrial high power living body breeding method of insect pathogen nematode. The present invention adopts living body breeding, rather than in vitro breeding, and has obviously raised agricultural pest invading power, high yield and efficiency, low cost, high quality and obviously raised pest preventing and controlling effect. The present invention may be used widely in preventing and controlling pest of potted crop, economic crop, vegetable, pasture, etc.

The invention aims to establish a preparation method of an entomopathogenic nematode insect corpse agent, and provides a novel application method of entomopathogenic nematodes in the field. In view of the limitations of low activity and intolerance of nematode to drying UV irradiation in direct application of entomopathogenic nematodes in the field, the invention uses insects (Galleria mellonella etc.) as a carrier; the host insect Galleria mellonella is infected by an entomopathogenic nematodes aqueous suspension in a laboratory; 36h later, the Galleria mellonella are all died and prepared into an insect corpse agent; and the insect corpse agent is then propagated at room temperature for at least 4 days, and directly applied in the field to control plant underground pests (Chinese chive maggot, Bradysia odoriphaga), so as to replace a traditional chemical pesticide to control underground pests and reduce pesticide investment. The invention effectively solves the defects of direct application of entomopathogenic nematodes, and has advantages of easy manufacture, convenient application, obvious effect and benefit to popularization.

The invention discloses a method for preventing and curing Bradysia odoriphaga by jointly applying entomopathogenic nematodes and chemical pesticides and belongs to the technical field of biological control and chemical control. At the 3-4 days old of the Bradysia odoriphaga, an entomopathogenic nematode resin agent and one-eighth (recommended dosage) of the chemical pesticides, which are safe to the nematodes, are jointly used to prevent and cure the Bradysia odoriphaga. According to the control method disclosed by the invention, by jointly using biological control and chemical control, the amount of use of the entomopathogenic nematodes and the chemical pesticides is reduced, the cost of prevention and cure is reduced, the control effect of the entomopathogenic nematodes and the chemical pesticides on the Bradysia odoriphaga is improved, and thus the aim of efficiently and safely preventing and curing the Bradysia odoriphaga is achieved.

The invention relates to a method for improving a control effect of entomopathogenic nematodes on Chinese chive maggots. The method comprises the steps that thiamethoxam diluent is mixed with entomopathogenic nematodes which accord with detection conditions to prepare synergistic suspension, the concentration of the nematodes in the synergistic suspension ranges from 500 IJS/mL to 1000 IJS/mL, and the concentration of thiamethoxam ranges from 12 mg/L to 15 mg/L; the synergistic suspension is applied to killing of Chinese chive maggot larva when the Chinese chive maggots are in the larval phase, and normal field management is conducted after application is conducted; under field conditions, the method of adding low-concentration thiamethoxam for utilizing the entomopathogenic nematodes to control the Chinese chive maggots is adopted, the control effect of the entomopathogenic nematodes on the Chinese chive maggots of vegetables such as Chinese chives, green Chinese onions, shallots and garlic which are prone to being damaged by the Chinese chive maggots can be significantly improved, an obviously synergistic effect of the nematodes and the thiamethoxam on control of the Chinese chive maggots is embodied, usage amount of the thiamethoxam and other pesticides are significantly decreased, the baiting technique of a black sticky trap on imagoes is combined, the initial population number of a field is effectively decreased, and the purpose of controlling the Chinese chive maggots in a safe and nuisanceless mode can be really achieved.

The invention discloses a solid culture method for entomopathogenic nematodes. The method is characterized by comprising the specific steps: (a) preparing an inoculum culture medium, activating symbiotic bacteria of the entomopathogenic nematodes, and inoculating the symbiotic bacteria to the inoculum culture medium; (b) inoculating a bacterial solution of the symbiotic bacteria to a solid culture medium; (c) carrying out culture for 3 to 5 days in the solid culture medium, and then, inoculating an entomopathogenic nematode suspension to the solid culture medium; (d) carrying out dark static culture on the nematodes for three weeks, thereby harvesting the nematodes. According to the method, the problem that bioculture methods cannot achieve the large-scale production of the entomopathogenic nematodes is solved, the production cost of the solid culture method is obviously lower than that of a liquid culture method, and the pathogenicity of the nematodes produced by the solid culture method is apparently higher than that of the nematodes produced by the liquid culture method. The market demand is increased, and the solid culture method has better prospects of development and has an all-important significance in the large-scale production of the entomopathogenic nematodes and the biological control on soil insects.

The invention discloses a method for preventing and treating soil insects by entomopathogenic nematodes. The method is characterized by comprising the specific steps: (a) preparing an entomopathogenic nematode suspension; (b) applying the entomopathogenic nematode suspension at a larval stage of the soil insects in a manner of enabling the entomopathogenic nematode suspension to be uniformly distributed to the land surface of crop soil; and (c) infecting larvae of the soil insects in the soil by the entomopathogenic nematodes in the entomopathogenic nematode suspension, so as to prevent and treat the soil insects. The method is a pollution-free natural-enemy biological control technology and has the characteristics of being convenient in application, high in host-searching capability, high in infected killing effectiveness, high in effect taking speed, safe to human and livestock, natural enemies and environments and the like, the defects of the traditional microbial agent biopesticides that the effect taking speed is low, resistance is easy to generate, the soil insects cannot be prevented and treated, and the like are broken through, and the soil insects can be prevented and treated instead of high-toxicity high-residue chemical pesticides, so that the method not only has ecological significance, but also has important environment-friendly significance.

The invention discloses an entomopathogenic nematode symbiotic bacteria strain which is characterized in that the class of the microorganism is named high-virulent strain R187-2 of serratia nematodiphila and collected in the China Center for Type Culture Collection on May 3rd, 2016, wherein the strain collection number is CCTCC M 2016240. The invention also discloses a biological insecticide as well as a preparation method and application thereof. The novel entomopathogenic nematode symbiotic bacteria insecticidal bacterial agent provided by the invention has the advantage of convenient production and use and is suitable for producing green pollution-free agricultural products in the modern ecological agriculture economy. The entomopathogenic nematode symbiotic bacteria strain is of great significance to protecting the ecological environment and improving the added value of agricultural products. Experiments prove that the liquid bacterial agent and solid bacterial agent of the R187-2 strain realize a good insecticidal effect, and the death rate of each group is 85% or more. With a wide insecticidal spectrum, the entomopathogenic nematode symbiotic bacteria strain R187-2 has a huge market development potential.

The invention discloses an entomopathogenic nematode symbiotic bacteria strain for inhibiting various plant fungal diseases and application. The bacteria strain is a high-toxicity bacteria strain NN6of xenorhabdus bovienii, the preservation number is CCTCC NO: M 2020097, and the bacteria strain is preserved in China Center for Type Culture Collection on April 29, 2020. The xenorhabdus bovienii NN6 disclosed by the invention has relatively strong ability of inhibiting growth of fusarium oxysporum f.sp.niveum, fusarium oxysporium f.sp.cucumerinum, fusarium graminearum, pyricularia grisea, botrytis cinerea and phytophthora capsici hypha, the ability is increased along with the increase of volume concentration, and the antibacterial effect is obvious. The bacteria strain also has the effect of inhibiting the germination of fusarium spores, and the inhibition effect is remarkable. The bacteria strain has the characteristics of low culture condition requirement, long lasting period, environment-friendly and non-toxic source and the like, has important significance in protecting the ecological environment and improving the added value of agricultural products, and has good market development and application prospects due to the advantage of wider antibacterial spectrum.

The invention discloses a method for controlling phyllotreta striolata fabricius through entomopathogenic nematodes. The method is characterized by particularly comprising the steps that 1, an entomopathogenic nematode suspension is prepared; 2, a controlling chemical is prepared; 3, soil moisture is kept, and the controlling chemical is applied; 4, an insect-proof screen is arranged for blocking, and the controlling chemical is put on the insect-proof screen regularly. The method is a nuisanceless natural enemy organism controlling technology and has the advantages of being convenient to apply, high in host seeking capacity and effectiveness of infection to death, rapid in onset time, safe to people and livestock, natural enemies and environments and the like, the problem that at present, ordinary chemicals cannot control larvae of the phyllotreta striolata fabricius is broken through, high-toxic and high-residue chemical insecticide can be replaced, and both ecological significance and important environment protection significance are achieved.

Biological pesticide, based on chitosane and entomopathogen nematodes. The invention consists of a new pesticide formulation, bio-stimulating and with fungicide effects, combining the bio-stimulating action due to chitosane to the biological control of plagues in agricultural and forest crops, due to phytopathogen insects by entomopathogen nematodes of the Steinernematidae and Heterorhabditidae families. There is a synergic action between the bio-stimulating and the biological pesticide due to the action of symbiotic bacteria of the Xenorhabdus and Photorhabdus genres, carrying the nematodes of these families. The aforementioned action is synergically enhanced by the bio-stimulating effect of chitosane over plants, on favouring the radicular development and degree of lignification and provoking the elicitation of phytoalexins producer genes, as a defensive mechanism.

The invention provides a method for the refrigerated storage of heterorhabditis bacteriophora entomopathogenic nematodes in cold areas, which relates to the technical field of biological refrigerated storage and solves the problems of short storage period, difficult long-distance transportation, low survival rate and low pathogenicity of the conventional low temperature (over 0 DEG C) storage of entomopathogenic nematodes. The method comprises the following steps of: 1) preparing storage media and storage bags; 2) inoculating; 3) performing refrigerated storage at different temperatures; 4) defreezing; and 5) isolating nematodes. The method for the refrigerated storage of the heterorhabditis bacteriophora entomopathogenic nematodes in the cold areas performs the refrigerated storage at the different temperatures on the heterorhabditis bacteriophora entomopathogenic nematodes which are peculiar to high-cold areas, breaks the limitations of current production scale and application areas of the entomopathogenic nematodes to ensure a storage period of 5 to 6 months, a rate of the recovering of the nematodes from the storage medium of 90 percent, a defrozen nematode survival rate of over 80 percent and a soil insect prevention and control effect of over 80 percent, solves the problems of unfavorable long-distance transportation and obviously decreased pathogenicity of the nematodes which are stored at the temperature of over 0 DEG C for long time and achieves a good effect of biologically preventing and controlling destructive insects in agricultural productive practice.

The invention provides a method for improving entomopathogenic nematodes infection capability and relates to an entomopathogenic nematodes infection method. The invention aims at solving the problem of low infection level of entomopathogenic nematodes on mealworm. The method is concretely completed according to the following steps that: firstly, a mealworm constant temperature culture box is subjected to dry heat treatment, then, the mealworm subjected to the dry heat treatment is placed into injection period entomopathogenic nematodes suspension to be soaked, and the infection of the entomopathogenic nematodes on the mealworm is completed. The method has the advantage that the injection rate of the mealworm is improved from 55 to 57 percent to 95 to 98 percent. The method is mainly used for the injection of the entomopathogenic nematodes on the mealworm.

The present invention relates to active compound combinations and compostions for reducing the damage of plants and plant parts as well as losses in harvested fruits or vegetables caused by insects, nematodes or phytopathogens and which have fungicidal or nematicidal or insecticidal activity including any combination of the three activities, in particular within a composition, which comprises (A) Fluopyram, (B) a spore-forming bacterium of the genera Bacillus, selected from Bacillus firmus, Bacillus cereus, Bacillus pumilis, Bacillus amyloliquefaciens, Bacillus subtilis strain GB03, Bacillus subtilis strain QST713, and (C) at least one biological control agent, in particular bacteria, fungi or yeasts, protozoa, viruses, entomopathogenic nematodes, inoculants, botanicals and products produced by microorganisms including proteins or secondary to metabolites. Moreover, the invention relates to a method for curatively or preventively controlling insects, nematodes or phytopathogens on the plant, plant parts, harvested fruits or vegetables, to the use of a combination according to the invention for the treatment of seed, to a method for protecting a seed and not at least to the treated seed.

The invention discloses application of heterorhabditis bacteriophora to biological control of underground insects of greenhouse vegetables. The heterorhabditis bacteriophora is obtained through separation, and fresh chicken internal organs are adopted or chicken gizzards and gallbladders are removed; 20%w/v of water and 10%-20% of vegetable oil are added, and the mixture is homogenized and sterilized to be used as a culture medium for culturing and breeding entomopathogenic nematodes; nematode suspension liquid with the concentration of 10000Ijs per seedling is prepared and is filled to roots of the seedlings; after application, the fatality rate of mosquito and fly larvae is 100%, and the fatality rate of large-size underground insects including grubs, mole crickets and the like is 50% or more; the heterorhabditis bacteriophora has the acting effects of no toxin and no pollution, and capability of keeping a long effect after one time of application; the heterorhabditis bacteriophora has active searching and expanding propagation capabilities, so that the heterorhabditis bacteriophora has the acting effect on underground insect larvae which are difficult to prevent and control and the acting effect is better than that of other medicines; and the heterorhabditis bacteriophora has a wide application prospect on production of green and healthy greenhouse vegetables.

The invention relates to a symbiotic bacterium of entomopathogenic nematode and an application of the symbiotic bacterium. The bacterium is xenorhabdus budapestensis HEB02, which is preserved in China General Microbiological Culture Collection Center on No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing on April 11, 2016, with preservation number of CGMCC No.12352. The bacterium is used for controlling soybean soreshin blight. The xenorhabdus budapestensis HEB02 provided by the invention is relatively strong in capacity of inhibiting promycelium growth of the soybean soreshin blight; and as increase in volume concentration, an antibacterial effect becomes more obvious.

The invention relates to a method for controlling black cutworm by combining entomopathogenic nematodes with matrine. The method comprises the following steps: adding water to dilute matrine in order to prepare a diluted matrine solution, and mixing the diluted matrine solution with entomopathogenic nematodes meeting detection conditions to prepare an entomopathogenic nematodes and matrine suspension, wherein the concentrations of the entomopathogenic nematodes and matrine in the suspension are 500-1000IJS/mL and 0.6-1.2mg/L respectively. The suspension is applied in the seedling stage of plants and the larval stage of the black cutworm in order to kill the black cutworm, and normal field management is carried out after the application. The control method improves the black cutworm control effect and reduces the dosages of the entomopathogenic nematodes and matrine in order to achieve the biological control of the black cutworm by reasonably utilizing the entomopathogenic nematodes.