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13 results about "Entomopathogenic nematode" patented technology

Entomopathogenic nematodes are a group of nematodes (thread worms), causing death to insects. The term entomopathogenic has a Greek origin entomon, refers to insect, and pathogenic, which denotes causing disease. They are multi-cellular metazoans that occupy a bio control middle ground between microbial pathogens and predator/ parasitoids, and are habitually grouped with pathogens, most likely because of their symbiotic relationship with bacteria. Although many other parasitic thread worms cause diseases in living organisms (sterilizing or otherwise debilitating their host), entomopathogenic nematodes, are specific in only infecting insects. Entomopathogenic nematodes (EPNs) live parasitically inside the infected insect host, and so they are termed as endoparasitic. They infect many different types of insects living in the soil like the larval forms of moths, butterflies, flies and beetles as well as adult forms of beetles, grasshoppers and crickets. EPNs have been found in all over the world and a range of ecologically diverse habitats. They are highly diverse, complex and specialized. The most commonly studied entomopathogenic nematodes are those that can be used in the biological control of harmful insects, the members of Steinernematidae and Heterorhabditidae (Gaugler 2006). They are the only insect-parasitic nematodes possessing an optimal balance of biological control attributes. (Cranshaw & Zimmerman 2013).

Pickering emulsion for coating entomopathogenic nematodes

PendingUS20260000085A1BiocideMaterial nanotechnologyBiotechnologyInsect disease
Provided herein is a composition comprising a Pickering emulsion comprising a plurality of core-shell particles encapsulating an entomopathogenic organism. Furthermore, a method for controlling a pest on or within a plant or at the area under cultivation is provided.
Owner:THE STATE OF ISRAEL MINISTRY OF AGRICULTURE & RURAL DEVELOPMENT +2

Method for mass production of entomopathogenic nematodes used as a biological control agent

UndeterminedPK141447AJuvenileEntomopathogenic nematode
This invention relates to method, in vivo and in vitro for mass production of entomopathogenic nematodes as a biological control agent for laboratory use and small scale field application against agricultural pest. In vivo production has managed to sustain as a cottage industry. In vivo production is likely to continue as small business venture for niche markets. For in vivo production eight Pakistani strains of entomopathogenic nematodes were mass cultured using G. mellaonella larvae at different concentrations and in vitro production were carried out in soya and wheat flour medium. Average in vivo production potential at were 14 x 106 infective juveniles and in vitro production potential ranged up to 50* 10 6 inefective juveniles.
Owner:SALMA JAVED

Insecticidal nematode proteins and methods of use thereof

PendingCN121843957ABiocideClimate change adaptationBiotechnologyOral toxicity
As disclosed herein, insect parasitic nematodes are considered as "beneficial nematodes" as they kill pests, produce pesticidal proteins and release them into their hosts. These pesticidal proteins have oral toxicity to certain pests. The present disclosure provides methods for identifying these proteins and provides examples of proteins having pesticidal activity, such as oral toxicity to various pests.
Owner:RGT UNIV OF CALIFORNIA

Methods And Compositions For Increasing Infectivity Of Entomopathogenic Nematodes

PendingUS20260076373A1BiocideAnimal repellantsBiotechnologyEntomopathogenic nematode
Disclosed are methods for increasing infectivity of entomopathogenic nematodes in any target area for the purposes of insect control, said method comprising:(a) mixing an aqueous nematode infectivity increasing composition with (i) entomopathogenic nematodes to activate said entomopathogenic nematodes for increased infectivity prior to application of said entomopathogenic nematodes to said target area, or (ii) seeds to produce coated seeds, and(b) applying said entomopathogenic nematodes to said target area or planting said coated seeds in said target area;wherein said aqueous nematode infectivity increasing composition is produced by methods disclosed herein.
Owner:PHERONYM INC +1

Combined product for preventing and treating potato tuber moth and use method thereof

The invention belongs to the technical field of agriculture, and particularly relates to a combined product for preventing and treating phthorimaea operculella and a use method. The combination product comprises a phthorimaea operculella repellent, an entomopathogenic nematode corpse compound agent and a female phthorimaea operculella oviposition inducing device, the repellent is a microcapsule coating an active component; the female phthorimaea operculella oviposition inducing device comprises a solar automatic black light lamp and a lamp arm unit; a water retention carrier soaked with a chemical attractant is placed on a tray of the lamp arm unit; and fresh potato tubers treated by the insect feeding stimulant are arranged above the water retention carrier. The invention further discloses a using method of the combined product. The three-in-one synergistic prevention and control strategy is adopted, a three-dimensional protection system covering the underground, plants and the air is constructed through three components with complementary functions, use of chemical pesticides is reduced, all life history stages of pests are blocked through cooperation of different action sites, and sustainable green comprehensive treatment is achieved.
Owner:INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI

Application of butylated hydroxytoluene in enhancing biocontrol activity of entomopathogenic nematodes

The invention discloses an application of butylated hydroxytoluene in enhancing the biocontrol activity of entomopathogenic nematodes. The entomopathogenic nematodes are pretreated by using the volatile butylated hydroxytoluene specifically released by the larvae of the red weevil, so that the activity of the entomopathogenic nematodes can be remarkably enhanced, and the quantitative enhancement of the behavior of the nematodes can be achieved; therefore, the parasitic lethal ability of entomopathogenic nematodes to hosts (larvae such as red palm weevils, greater wax moths and tomato leaf miners) is improved, and the control effect on various pests is effectively improved. Butylated hydroxytoluene has no toxic or side effect on entomopathogenic nematodes under an effective concentration, so that the survival and activity stability of the entomopathogenic nematodes are ensured. The invention provides a safe, efficient and wide-application-range strategy for improving biological prevention and control means based on entomopathogenic nematodes, and has good popularization and application prospects.
Owner:FUJIAN AGRI & FORESTRY UNIV

Method for improving heavy metal stress harm resistance of entomopathogenic nematodes

The invention discloses a method for improving resistance of entomopathogenic nematodes to heavy metal stress hazards, which is characterized in that nano-zinc oxide (nano-ZnO) is used for treating entomopathogenic nematodes, so that the tolerance of the entomopathogenic nematodes to heavy metals (taking Cd as an example) is remarkably enhanced, and the Cd stress death rate of the entomopathogenic nematodes within 12 hours is reduced from 96.74% of untreated entomopathogenic nematodes to 11.09%. The diffusivity is increased to 78.69% from the untreated 47.56%, the aggregation rate within 4 hours is increased to 28.05% from the untreated 18.45%, and the invasion rate within 12 hours is increased to 9.85% from the untreated 6.10%. The pathogenicity to the pests is also remarkably enhanced, and the death rate of the pests within 108 hours is increased to 100% from 82.00% which is not treated. The method is easy to operate, low in cost and environmentally friendly, provides a new feasible thought and method for application of the entomopathogenic nematodes in the heavy metal contaminated soil, and has a wide application prospect.
Owner:GUIZHOU UNIV

Pest control kit and method

A pest control kit is provided to aid the control of a population of immature larval stages or pupal insects. The pest control kit comprises an amount of an entomopathogenic nematode and a wetting agent composition, the wetting agent composition comprising: a solvent; and a surfactant. The pest control kit aims to provide a naturally occurring entomopathogenic nematode for biological control of immature larval insects which can act as pests to plants, whether these be for professional plant protection including agricultural, forestry and horticultural or for home garden use. The new pest control product of the present invention aims to prove safe and sustainable protection for turf and plants all year round.
Owner:SYNGENTA CROP PROTECITON AG

Efficient method for mass rearing of entomopathogenic nematodes

The application belongs to the field of biological agents, and particularly relates to a high-efficiency trapping method of insect pathogenic nematodes, which adopts Galleria mellonella treated by immune barrier weakening and cocooning inhibition in sequence for trapping and collecting; and specifically comprises the following steps: S1, collecting fresh soil samples from the wild; S2, inoculating biocontrol bacteria into the soil samples, spraying pheromones and moisturizers, and mixing; S3, placing the Galleria mellonella treated in the above step into a trapping agent ball, and filling the soil samples treated in the step S2 into the trapping agent ball and compacting; S4, burying the trapping agent ball in the remaining soil samples, and after completion, placing the soil samples in a sample bag in a thermostat with sufficient oxygen for trapping, the application can improve the success rate of nematode infection, is beneficial to further propagation, collection and application of insect pathogenic nematode resources, and has the advantages of simple method and simple operation.
Owner:INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI

A process of preparation in vivo and in vitro methods for mass production of entomopathogenic nematodes as a biological control agent, alternative to chemical control

UndeterminedPK201100459A0Chemical controlMicrobiology
This invention relates to method, in vivo and in vitro for mass production of entomopathogenic nematodes as a biological control agent for laboratory use and small scale field application against agricultural pest. In vivo production has managed to sustain as a cottage industry. In vivo production is likely to continue as small business venture for niche markets. For in vivo production eight Pakistani strains of entomopathogenic nematodes were mass cultured using G. mellaonella larvae at different concentrations and in vitro production were carried out in soya and wheat flour medium. Average in vivo production potential at were 14 x 106 infective juveniles and in vitro production potential ranged up to 50* 10 6 inefective juveniles.
Owner:SAHINA FAYYAZ M H SOOMRO +1

Entomopathogenic nematode bioassay device

The entomopathogenic nematode bioassay device comprises a bioassay plate 1, a bioassay cover 15, a gradienter 8, a sample plug 11 and a degumming brush 18, the bioassay plate 1 is provided with a bioassay area 2, an identification area 13 and a bioassay area number 3, and shading 17 is arranged at the bottom of the bioassay plate 1; the bioassay area 2 is provided with a cover groove 4 and a bioassay pool 6; the bioassay pool 6 is provided with ruler scales 7 and an indicating arrow 10; the bioassay pool 6 is provided with a height indicating line 14 and a sample adding hole 5; the gradienter 8 is provided with an indicating bubble 9; the sample plug 11 is provided with a plug head 12; finger anti-skid areas 16 are arranged on the two sides of the bioassay cover 15; the glue removing brush 18 is provided with a brush handle 19, brush handle anti-skid lines 20 and a brush head 21. The device has the advantages of being simple in structure, convenient to operate, high in working efficiency, wide in application range and the like, and has good practicability.
Owner:QINGDAO AGRI UNIV +1