103 results about "Action sites" patented technology

The invention provides compositions of novel high penetration compositions (HPC) or high penetration prodrugs (HPP) of retinoids and retinoid-related compounds, which are capable of crossing biological barriers with high penetration efficiency. The HPPs are capable of being converted to parent active drugs or drug metabolites after crossing the biological barrier and thus can render treatments for the conditions that the parent drugs or metabolites can. Additionally, the HPPs are capable of reaching areas that parent drugs may not be able to access or to render a sufficient concentration at the target areas and therefore render novel treatments. The HPPs can be administered to a subject through various administration routes, e.g., locally delivered to an action site of a condition with a high concentration or systematically administered to a biological subject and enter the general circulation with a faster rate.

The invention relates to a pesticide, in particular to a compound bactericide for mainly treating grey mould of fruits and vegetables. The compound bactericide consists of active ingredients, padding and / or an auxiliary agent, wherein the active ingredients consist of Fludioxonil and other bactericide in a weight proportion of 1-50:1-50, and other bactericide is one or mixture of Boscalid, Pyrimethanil, Procymidone, Pyraclostrobin and chlorothalonil. The product made of the compound bactericide for mainly treating the grey mould of fruits and vegetables is generally wettable powder, water dispersible granules and suspending agent. The compound bactericide is suitable for preventing and controlling grey mould of various fruits and vegetables, has remarkable synergic effect, long persistence, novel action mechanism and more action sites, and can effectively delay the occurrence and development of drug resistance of grey mould bacteria. The compound bactericide is simple in preparation method, and has high biological safety and remarkable economic benefit.

The invention discloses a monoclonal antibody of a human anti-Ebola virus envelope glycoprotein GP. The antibody has a unique CDR region and has a unique action site. Computer simulation analysis and biological experiments confirm that the antibody acts on the NPC1 receptor binding region of the GP, and also acts on the glycan cap region of the GP, so the antibody plays a role in the anti-Ebola virus infection through the unique neutralizing mechanism formed by the combined action of the antibody on the two structural domains of the GP. The antibody has high antigen binding activity and neutralizing activity, can protect 50% of virus attacked cells just at dose of 0.3 [mu]g / ml, and has a protection rate reaching 100% at an antibody concentration of 100 [mu]g / ml, so the antibody provided by the invention has a wide application prospect in the preparation of Ebola virus disease treatment medicines.

The present invention relates to toxoid preparations comprising a non-disrupted and / or a non-denatured toxin associated with a particulate vector that minimizes or precludes said toxin from inflicting damage at an action site of said toxin. The present invention also relates to immunogenic compositions or vaccines comprising the toxoid preparations, and the methods of using the toxoid preparations, immunogenic compositions or vaccines.

The invention discloses a 25,27-di(3-methyl-thio-ethoxy thiadiazole) calix[4]arene stationary phase which is low in cost, high in content of compounded calixarene bonded stationary phase bonded amount, and stable in stability bonding layer, and is prepared by the following steps of: under the action of phase transfer catalyst ammonium tetrabutyl bromide, carrying out nitrogen protection to gamma-glycidol ether bonded silica gel and calixarene derivative sodium phenolate, and preparing the object calixarene stationary phase at 80 DEG C. The calixarene stationary phase compounded by the method in the invention is high in bonded amount, stable in boned layer, simple and easy in compounding method, low in cost, free of pollution in the compounding process and the like. The stationary phase not only has traditional ODS (Octadecylsilyl) reversion phase chromatography performance, and has containing complexing action, pi-pi action, hydrogen bonding action and dipole-dipole action because of many action sites; in a certain degree, the stationary phase in the invention has special separation performance comparing with the traditional ODS column.

The invention discloses a bactericidal composition, a bactericidal pesticide and application of the composition and the pesticide. The composition comprises thifluzamide and azoxystrobin of which the weight ratio is (10:1) to (1:10). The pesticide comprises the composition used as an effective component of the pesticide and auxiliary components of the pesticide. The invention further provides application of the composition to the control of rice sheath blight disease. Compared with a single dosage, the bactericidal composition has a remarkable synergistic effect on the disease, improves the disinfection effect, reduces pesticide use times, reduces pesticide use cost, and reduces environmental pollution and pesticide residue; as the composition comprises effective components with different modes of actions, action sites are increased, which is conducive to eliminating and delay of the generation of drug resistance of germs.

The invention belongs to the field of agricultural herbicide, and in particular relates to a composite herbicide. The composite herbicide contains diflufenican and pendimethalin which serve as active ingredients and have a weight ratio of (10:1)-(1:20). The composite herbicide has the advantages that: 1, the synergistic effect is obvious, and the pesticide effect is greatly improved; 2, the pesticide effect is improved, the field consumption of the active ingredients is reduced, the production and use costs are reduced, and pesticide residue and environmental pollution are reduced; and 3, the composite herbicide consists of the active ingredients with different action mechanisms, the action sites are increased, pesticide resistance of weeds are overcome and delayed, and the composite herbicide is an important means for comprehensively preventing and controlling field weeds.

The invention discloses a tumor gene mutation detection method based on RNA-seq data. The method comprises the following steps that gene mutations for different high-incidence tumors and diseases arecollected and sorted so as to build a high-priority mutation detection gene list and a mutation site database; gene mutations currently having specific targeted or chemotherapeutic drug action sites and gene database are collected and sorted; a gene mutation annotation database based on RNA-seq is built; quality control, gene sequence alignment and gene mutation detection algorithms used for RNA-seq mutation detection are collected and sorted; and a set of complete analysis process from initial RNA-seq sequencing data to final mutation detection result is built. The invention further disclosesa tumor gene mutation detection system based on RNA-seq data. By using the integrated multiple databases, people can more rapidly find the known gene mutation sites related to tumorigenesis through the detection analysis process.

The invention belongs to the technical field of agricultural insecticides, and particularly relates to a Dinotefuran / Spirotetramat compound insecticidal composition. The effective ingredients are Dinotefuran and Spirotetramat in a weight ratio of 3:1-1:3. The Dinotefuran / Spirotetramat compound insecticidal composition is composed of the effective ingredients with different action mechanisms, so that the action sites are increased, thereby being beneficial to overcoming and postponing the generation of insecticide resistance of insects, and widening the insecticidal spectrum; and the invention greatly enhances the insecticidal effect, has the advantages of long preventive effect time and lower consumption of effective ingredients in the field, lowers the production and use cost, and reduces the insecticide residue and environmental pollution.

The invention discloses a pyrimorph-containing bactericide composition. The bactericide composition is characterized in that effective constituents are pyrimorph (A) and one (B) of trimanin, chlorthalonil, metalaxyl-M, metalaxyl and fosetyl-aluminium at a weight ratio of 1:90-90:1. The effective constituents A and B of the composition are combined rationally, and have advantages of obvious synergic action, protective and therapeutic actions, excellent quick efficiency and long duration. The composition solves problems of single action site, large use amount and easily forming drug resistance in use of a single bactericide, meanwhile, the composition reduces risk of chemical injury due to excessive single bactericide and can be widely applied to control of vegetable downy mildew, epidemic disease and downy mildew of fruit trees.

The invention belongs to the field of agricultural herbicide, and in particular relates to a compound herbicide. The compound herbicide contains diflufenican and oxyfluorfen which serve as active ingredients and a weight ratio of the diflufenican and oxyfluorfen is (10:1)-(1:10). The compound herbicide has the advantages that: 1, the synergistic effect is obvious, and the pesticide effect is greatly improved; 2, the pesticide effect is improved, the field consumption of the active ingredients is reduced, the production and use costs are reduced, and pesticide residue and environmental pollution are reduced; and 3, the compound herbicide consists of the active ingredients with different action mechanisms, the action sites are increased, pesticide resistance of weeds are overcome and delayed, and the compound herbicide is an important means for comprehensively preventing and controlling field weeds.

The invention provides compositions of novel high penetration compositions (HPC) or high penetration prodrugs (HPP) of peptides and peptide-related compounds, which are capable of crossing biological barriers with higher penetration efficiency comparing to their parent drugs. The HPPs are capable of being converted to parent drugs or drug metabolites after crossing the biological barrier and thus can render treatments for the conditions that the parent drugs or metabolites can. Additionally, the HPPs / HPCs are capable of reaching areas that parent drugs may not be able to access or to render a sufficient concentration at the target areas and therefore render novel treatments. The HPPs / HPCs can be administered to a subject through various administration routes, e.g., locally delivered to an action site of a condition with a high concentration or systematically administered to a biological subject and enter the general circulation with a faster rate.

The invention discloses a zinc-binding protein action site prediction method based on integrated learning in a non-equilibrium mode. According to characteristics of the zinc-binding protein action site, protein source data is pre-processed; non-equilibrium of the zinc-binding protein site is equilibrated through the random down-sampling technology to obtain several sub-equilibrium data sets; for the sub-equilibrium data sets, distinguishable protein biochemical characteristics are selected for characteristic representation to form characteristic vectors; the characteristic vectors are respectively taken as the input of a base classifier support vector machine to calculate the sample weight, a probability neural network model based on the sample weight is constructed, and lastly, the base classifier support vector machine and the probability neural network model based on the sample weight are integrated to obtain a prediction model; the prediction model is utilized to identify the zinc-binding protein action site of a target sample.

The invention provides a multi-effect repair polypeptide composition and application thereof in cosmetics. The polypeptide composition comprises oligopeptide-1, carnosine, blue copper peptide, palmitoyl tetrapeptide-7, acetyl hexapeptide-8 and nonapeptide-1. The invention compounds the six polypeptides for the first time, and the mechanism and the action sites of the polypeptide components for playing a repairing action are different, so that the components not only can independently come into play aiming at different skin problems, but also can have synergistic effect aiming at the same skinproblem, can come into play by a combination of measures, can effectively repair the skin barrier, and can return the skin to a smooth, moist and elastic health state. The polypeptide composition of the present invention is added to cosmetics. The obtained cosmetics have comprehensive repair effects of moisturizing skins, resisting oxidation, removing speckle, resisting wrinkle and relieving sensitive stimulation, and can enhance glossiness and firmness of skin and restore health of skin.

The invention relates to an agricultural sterilization combination. The agricultural sterilization combination contains phenylate diazole and cyprodinil which are (1:100)-(100:1) in weight ratio, preferably (1:20)-(20:1). The invention further relates to agricultural bactericides containing the agricultural sterilization combination and application of the agricultural bactericides to control of fruit tree botrytis and vegetable botrytis. The sterilization combination has remarkable synergistic effect, improves sterilization effect, reduces pesticide utilization cost, reduces environment pollution and pesticide residues, enables action sites to be increased, and is favorable for overcoming and delaying generation of pesticide resistance of germs. The sterilization combination is good in safety, and has good control effect on the fruit tree botrytis and the vegetable botrytis.

The invention discloses a bactericidal composition which comprises a composition body. Active components of the composition body are fludioxonil and prochloraz. The bactericidal composition has the advantages that the fludioxonil is a novel broad-spectrum non-internal-suction pyrrole bactericide, the prochloraz is a broad-spectrum imidazole bactericide with protection and eradiation effects, mechanisms for the prochloraz include inhibiting biological synthesis of germ ergosterol, special effects can be realized for diversified crop diseases due to ascomycetes and deuteromycota, the prochloraz does not have an internal suction property, but certain conduction effects can be realized by the prochloraz; the fludioxonil and the prochloraz have different action mechanisms and different action sites and are free of cross-resistance, and the pesticide resistance of germs can be prevented when the fludioxonil and the prochloraz are mixed with each other; synergistic effects further can be realized by the bactericidal composition, the pesticide application quantities can be reduced, and accordingly the service cost can be lowered; the fludioxonil is high in cost when used individually, but the cost can be obviously lowered when the fludioxonil and the prochloraz are mixed with each other on the premise that the efficacy is guaranteed.

The invention discloses an alpha-galactosidase gene and application thereof, and relates to the technical field of biology. The alpha-galactosidase gene is suitable for encoding alpha-galactosidase, and a nucleotide sequence is shown as SEQ ID NO:1. The artificially designed alpha-galactosidase gene has the advantages that the high-frequency codon in pichia pastoris is used for replacing the original low-frequency codon, so that the free energy of mRNA (messenger ribonucleic acid) and the complexity of mRNA secondary structure are decreased; by selecting the secondary high-frequency codon, the areas containing AT (adenine and thymine) or GC (guanine and cytosine) in the gene or the protease action site are eliminated, and the expression amount of the artificially synthesized alpha-galactosidase gene in the pichia pastoris cell is obviously increased.

The invention discloses a preparation method of a rooting agent for promoting winter sweet cutting. The method includes the steps of: preparing a ferrous chelating agent, compounding an auxin solution, and preparing a hydrogel rooting agent. According to the invention, the complex formed by an active polypeptide ferrous chelating agent and auxin can prevent auxin from photooxidation decomposition and lose of regulation effect, under appropriate conditions, decomplexation reaction can be carried out on the complex to release auxin and transform the auxin into free type, the free auxin can be transported to an action site to take effect, can prevent incisions from infecting pathogens and rotting, promote nursery stocks to generate calluses in advance, improve photosynthesis of flowers and trees, increase photosynthetic products, enhance water absorption and transportation functions, and reduce transpiration effect, thereby improving the cutting survival rate. The auxin hydrogel rooting agent is evenly applied on winter sweet cuttings, and a layer of thin auxin hydrogel rooting agent film can be formed after drying, thus being conducive to water maintaining and rooting agent release. Therefore, the rooting agent is an efficient winter sweet cutting rooting agent.

The invention relates to a method for preparing a graphene oxide modification column. The method specifically comprises the steps of preparation of a graphene oxide solution, pretreatment of a silica capillary column and modification of graphene oxide. The method disclosed by the invention has the advantages that the inner wall of the silica capillary column is modified by utilization of covalent interaction of the graphene oxide, so that not only can a capillary inner wall be modified as a stationary phase, but also an action site can be provided for separation of an analyte, and the separation capability of the capillary column can be improved.

The invention belongs to the technical field of agricultural insecticides and in particular relates to a dihalopyrazole amide and tolfenpyrad compound insecticidal composition. The active ingredients of the insecticidal composition are dihalopyrazole amide and tolfenpyrad in a weight ratio of 1 to (4-7), preferably 1 to 5. The insecticidal composition has the beneficial effects that the insecticidal composition is formed by the active ingredients with different mechanisms of action and the action sites are increased, so that the insecticidal composition is conducive to overcoming and delaying the drug resistance of insect pests and widening the insecticidal spectrums; the efficacy is substantially improved, the control effect duration is long, and the use quantities of the active ingredients in the fields are reduced, thus reducing the production and use costs and reducing the pesticide residues and environmental pollution.

The embodiments of the present invention disclose a protein feature extraction method, a functional model generation method, a function prediction method and corresponding devices. The protein featureextraction method includes the following steps that: the amino acid sequences of a target protein and residue action sites on the amino acid sequence of the target protein are acquired, so that the target amino acid sequence and target residue acting sites can be obtained; an amino acid abbreviation code or a plurality of consecutive amino acid abbreviation codes are intercepted from the target amino acid sequence, so that a target character string containing the target residue action sites is obtained; and the textual features of the target character string in the amino acid sequence are extracted. According to the methods and devices, the features of the residue action sites in the amino acid sequence are adopted as the expressions of protein functions, high-quality feature input can beprovided for subsequent protein function prediction by machine learning algorithms, the abandonment of time-consuming labor-consuming manual prediction methods can be benefitted, and the efficiency and accuracy of protein function prediction can be improved.

The invention provides a compound for enhancing space coupling degree of an endothelial cell ion channel complex TRPV4-KCa2.3 and application thereof in resisting hypertension. The compound has the advantages that by finding the structure domain of interaction sites of the endothelial cell ion channel complex TRPV4-KCa2.3, the specific compound which can simultaneously act on the two action sites is prepared; the space coupling degree of the complex TRPV4-KCa2.3 can be enhanced by the compound, and the important meaning is realized for the research and development of hypertension drugs.

The invention discloses a compound sterilizing agent and application thereof. The compound sterilizing agent consists of fluopicolide and fludioxonil with the mass ratio of (1-9): (1-9), the preferred mass ratio of (1-7): (1-5) or the more-preferred mass ratio of (1-5): (1-3). The compound sterilizing agent can be used for preventing and treating plant diseases such as vegetable downy mildew, late blight, gray mold, cucumber root rot and pepper phytophthora blight. The compound sterilizing agent disclosed by the invention is good in quick acting and persistence, has a unique action mechanism, more action sites and a wide bacteriocidal spectrum, is high in biosafety, simple in preparation process and low in cost and has obvious economic benefits.

The invention belongs to the technical field of tumor prevention and treatment and concretely relates to a Clec9a-targeting affinity peptide WH peptide and its preparation method and use. The peptide comprises 12 amino acids, has an amino acid sequence of WPRFHSSVFHTH and can be prepared through an Fmoc solid-phase synthesis method. The peptide can be used in tumor prevention and treatment. The peptide can be obtained through phage-displayed peptide library technology screening. The result of the research on an in-vivo and in-vitro foreign antigen cross presentation capacity and induction cases of specific CTL reaction shows that the WH peptide as a targeting carrier can well induce specific CTL response, improve IFN-gamma and related cell killing molecule expression quantity and improve tumor resistance. Through a computer simulated molecular docking technology, a possible docking area between the WH peptide and the Clec9a protein and action sites of the possible docking area can be researched. The peptide provides good reference and reference significance for related research.

The invention discloses a study method of Tet2 sequence preference, and the study method is characterized in that the Tet2 sequence preference is studied by amplified signal differences. According to the invention, small changes of Tet2 action site flanking sequences are performed with signal amplification based on the isothermal amplification technique, differences of main oxidation product contents are detected, so as to realize the study on sequence preference behaviors of Tet2 in the active demethylation process. The study method has the following characteristics: high-throughput detection, capable of achieving simultaneous detection of multiple samples; low detection cost, independent of expensive and complex large-scale instruments; good biocompatibility, unable to cause damage to protein or DNA samples; and simple operation, capable of achieving the transformation of protein behavior differences into amplified signal differences.

Belonging to the technical field of sample pretreatment and pollutant analysis and detection, the invention discloses an aqueous phase preparation method and application of a tylosin tartrate surfacemolecularly imprinted polymer. The technical scheme includes the key points that: 2-acrylamide-2-methylpropane sulfonic acid and / or 1, 4-butanediyl-3, 3'-bis-1-vinyl imidazolium chloride can be adopted as the functional monomer, tylosin tartrate is taken as the template molecule, pure water is adopted as the preparation solvent, the crosslinking agent N, N'-methylene bisacrylamide and the initiator azobisisobutyronitrile are utilized for thermal initiation of polymerization reaction to prepare the tylosin tartrate surface molecularly imprinted polymer on a styrene-divinyl benzene granular carrier surface. The tylosin tartrate surface molecularly imprinted polymer has action sites for specific recognition of tylosin tartrate, and can be used for selective adsorption of trace tylosin tartrate in environmental samples. According to the invention, the preparation process is simple and the cost is low, and the polymer adsorbent material has uniform particle size, large adsorption capacity and high mass transfer rate.

The invention relates to the field of cosmetics, and especially relates to a composition comprising an amide derivative and its use in cosmetics. The composition comprises: a. at least one amide derivative (A) represented by a formula (1); b. at least one resorcinol derivative (B) represented by a formula (2); and c. at least one cosmetically acceptable component (C). The amide derivative contained in the composition described in the present application has a positive reinforcing effect on the transdermal delivery of the composition, which facilitates the active substance of the composition toreach an action site through the skin of each layer, the composition prevents and / or inhibits changes in the appearance of discontinuities visible to the skin caused by melanin pigmentation, skin aging or other conditions, and is capable of treating, regulating or preventing oxidative stress or degradation of the skin, the composition also inhibits melanin generation and precipitation, reduces skin color and whitens the skin color, and keeps skin clear and white.

The invention provides compositions of novel high penetration compositions (HPC) or high penetration prodrugs (HPP) of antimicrobials and antimicrobial-related compounds, which are capable of crossing biological barriers with high penetration efficiency. The HPCs / HPPs are capable of being converted to parent active drugs or drug metabolites after crossing the biological barrier and thus can render treatments for the conditions that the parent drugs or metabolites can. Additionally, the HPPs are capable of reaching areas that parent drugs may not be able to access or to render a sufficient concentration at the target areas and therefore render novel treatments. The HPCs / HPPs can be administered to a subject through various administration routes, e.g., locally delivered to an action site of a condition with a high concentration or systematically administered to a biological subject and enter the general circulation with a faster rate.

A synergist for food biological preservatives and a use method of the synergist; the synergist is prepared from the following raw materials in percentage by weight: 15-20% of cellulase, 15-20% of lipase, 30-40% of protease, 10-20% of tea polyphenol, 10-20% of cinnamon extract. The use method of the synergist is as follows: the raw materials are weighed and mixed, and the food biological preservatives are mixed and dissolved for use with a weight ratio of 1:(20-30). The invention firstly starts from the new target spots of putrefying bacteria biofilm control and quorum sensing inhibition, and combines biofilm degrading enzyme and a quorum sensing inhibitor to interfere with the formation of biofilm of food putrefying bacteria and reduce the thickness of the putrefying bacteria biofilm, andinhibit the quorum sensing information exchange between putrefying bacteria, thereby improving the sensitivity of the putrefying bacteria to biological preservatives and enhancing the effectiveness ofbiological preservatives. The target spot of the synergist is the biofilm and quorum sensing system of the putrefying bacteria, and the action site is accurate, not a lethal effect in a simple sense;the putrefying bacteria does not produce resistance, and has the unparalleled superiority of the prior art.