The invention discloses a preparation method of a rooting agent for promoting winter sweet cutting. The method includes the steps of: preparing a ferrous chelating agent, compounding an auxin solution, and preparing a hydrogel rooting agent. According to the invention, the complex formed by an active polypeptide ferrous chelating agent and auxin can prevent auxin from photooxidation decomposition and lose of regulation effect, under appropriate conditions, decomplexation reaction can be carried out on the complex to release auxin and transform the auxin into free type, the free auxin can be transported to an action site to take effect, can prevent incisions from infecting pathogens and rotting, promote nursery stocks to generate calluses in advance, improve photosynthesis of flowers and trees, increase photosynthetic products, enhance water absorption and transportation functions, and reduce transpiration effect, thereby improving the cutting survival rate. The auxin hydrogel rooting agent is evenly applied on winter sweet cuttings, and a layer of thin auxin hydrogel rooting agent film can be formed after drying, thus being conducive to water maintaining and rooting agent release. Therefore, the rooting agent is an efficient winter sweet cutting rooting agent.