Synergist for food biological preservative and use method of synergist

A technology of biological preservatives and synergists, applied in the preservation of food ingredients as antimicrobials, food science, food preservation, etc. relationship and other issues, to achieve the effect of precise action site, inhibiting quorum sensing information exchange, and reducing thickness

Active Publication Date: 2018-12-21
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are few research reports on biofilm-degrading enzymes, and there i...

Method used

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  • Synergist for food biological preservative and use method of synergist
  • Synergist for food biological preservative and use method of synergist
  • Synergist for food biological preservative and use method of synergist

Examples

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Effect test

Embodiment 1

[0022] The product of the present invention is composed of the following raw materials in weight percentage: 20% of cellulase, 20% of lipase, 30% of protease, 12% of tea polyphenols, and 18% of cinnamon extract. The specific usage method is as follows:

[0023] (1) Weigh the raw materials according to the weight percentage, mix them uniformly to obtain a powdery mixture, put it into a clean food packaging container, seal it, and store it away from light;

[0024] (2) Take a certain weight of the mixed powder obtained in step (1), mix and dissolve it with chitosan at a weight ratio of 1:25.

[0025] (3) if figure 1 Shown, compared with using chitosan alone, the inhibitory rate of product of the present invention to chitosan inhibits Pseudomonas, Shewanella and Vibrio parahaemolyticus has improved respectively 60%, 15.9% and 186.7%, has Obvious synergy.

Embodiment 2

[0027] The product of the present invention is composed of the following raw materials in weight percentage: 20% of cellulase, 20% of lipase, 30% of protease, 12% of tea polyphenols, and 18% of cinnamon extract. The specific usage method is as follows:

[0028] (1) Weigh the raw materials according to the weight percentage, mix them uniformly to obtain a powdery mixture, put it into a clean food packaging container, seal it, and store it away from light;

[0029] (2) Take a certain weight of the mixed powder obtained in step (1), mix and dissolve it with polylysine at a weight ratio of 1:20.

[0030] (3) if figure 2 As shown, compared with using polylysine alone, the inhibitory rate of the product of the present invention to polylysine to inhibit Pseudomonas, Shewanella and Vibrio parahaemolyticus has increased by 180.5%, 108.3% and 91.7% respectively , with obvious synergistic effect.

Embodiment 3

[0032] The product of the present invention is composed of the following raw materials in weight percentage: 20% of cellulase, 20% of lipase, 30% of protease, 12% of tea polyphenols, and 18% of cinnamon extract. The specific usage method is as follows:

[0033] (1) Weigh the raw materials according to the weight percentage, mix them uniformly to obtain a powdery mixture, put it into a clean food packaging container, seal it, and store it away from light;

[0034] (2) Take a certain weight of the mixed powder obtained in step (1), mix and dissolve it with L-phenyllactic acid at a weight ratio of 1:25.

[0035] (3) if image 3 As shown, compared with using L-phenyllactic acid alone, the inhibition rate of the product of the present invention to L-phenyllactic acid inhibiting Pseudomonas, Shewanella and Vibrio parahaemolyticus has increased by 260.0%, 123.1% and 40.3% respectively , with obvious synergistic effect.

[0036] (4) Compared with using L-phenyllactic acid alone, the...

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Abstract

A synergist for food biological preservatives and a use method of the synergist; the synergist is prepared from the following raw materials in percentage by weight: 15-20% of cellulase, 15-20% of lipase, 30-40% of protease, 10-20% of tea polyphenol, 10-20% of cinnamon extract. The use method of the synergist is as follows: the raw materials are weighed and mixed, and the food biological preservatives are mixed and dissolved for use with a weight ratio of 1:(20-30). The invention firstly starts from the new target spots of putrefying bacteria biofilm control and quorum sensing inhibition, and combines biofilm degrading enzyme and a quorum sensing inhibitor to interfere with the formation of biofilm of food putrefying bacteria and reduce the thickness of the putrefying bacteria biofilm, andinhibit the quorum sensing information exchange between putrefying bacteria, thereby improving the sensitivity of the putrefying bacteria to biological preservatives and enhancing the effectiveness ofbiological preservatives. The target spot of the synergist is the biofilm and quorum sensing system of the putrefying bacteria, and the action site is accurate, not a lethal effect in a simple sense;the putrefying bacteria does not produce resistance, and has the unparalleled superiority of the prior art.

Description

technical field [0001] The invention belongs to the field of food preservation and food additives, and in particular relates to a synergist for food biological preservatives and a using method thereof. Background technique [0002] The toxic and side effects of traditional chemical preservatives such as sodium benzoate, sodium nitrite, and sodium diacetate have attracted increasing attention. Therefore, finding safe and non-toxic biological preservatives has become a hot spot of concern. The biological preservatives currently used in food mainly include chitosan, tea polyphenols, nisin, bamboo leaf antioxidants, rosemary extract, phytic acid, etc. At present, the biggest disadvantage of biological preservatives is the high cost, and the antiseptic effect is generally lower than that of chemical preservatives, which limits the use and promotion of biological preservatives. [0003] Bacterial quorum sensing system and bacterial biofilm are new targets for bacterial control i...

Claims

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Application Information

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IPC IPC(8): A23L3/3571A23L3/3472
CPCA23L3/3472A23L3/3571A23V2002/00A23V2200/10A23V2250/21A23V2250/2132A23V2250/214
Inventor 刘尊英董汝月曾名湧
Owner OCEAN UNIV OF CHINA
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