Dispersible trichoderma asperellum oil suspending agent and preparation method thereof

A technology of trichoderma aculeatus and dispersing oil, which is applied in the field of suspension agent fungicides, can solve the problems of storage resistance of trichoderma aculeatus spores, and achieve the effects of excellent permeability and adhesion, broad application prospects, and long storage time

Active Publication Date: 2014-08-13
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention will solve the technical problem that the existing Trichoderma aculeatus spores are not resistant to storage; and provide an environment-friendly, multi-applying Trichoderma aculeatus dispersible oil suspension and a preparation method thereof, to enrich the content of Trichoderma biological preparations type

Method used

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  • Dispersible trichoderma asperellum oil suspending agent and preparation method thereof
  • Dispersible trichoderma asperellum oil suspending agent and preparation method thereof
  • Dispersible trichoderma asperellum oil suspending agent and preparation method thereof

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specific Embodiment approach 1

[0022] Specific embodiment one: in the present embodiment, 3% Trichoderma aculeatus dispersible oil suspension formulation is shown in Table 1:

[0023] Table 1 Trichoderma aculeatus dispersible oil suspension formulation 1

[0024]

[0025] The preparation method of Trichoderma aculeatus dispersible oil suspension is as follows: add emulsifier, antioxidant, stabilizer, preservative, thickener, wetting dispersant, UV protection agent in vegetable oil, with 1500 r / min Stir with a magnetic stirrer at a high speed for 10 min, then homogenize the pretreated mixed solvent through a homogenizer, the working pressure is 1000 bar, and mix evenly to obtain a mixture (the particle size is controlled at about 25 μm); at room temperature, the mixture is obtained Mix it with conidia powder of Trichoderma aculeatus, add it to a sand mill, and grind for 20 minutes to obtain Trichoderma aculeatus dispersible oil suspension.

[0026] The preparation method of Trichoderma aculeatus conidia ...

specific Embodiment approach 2

[0029] Specific embodiment two: in the present embodiment, 7% Trichoderma aculeatus dispersible oil suspension formulation is as table 2:

[0030] Table 2 Trichoderma aculeatus dispersible oil suspension formulation II

[0031]

[0032]In this embodiment, the preparation method of Trichoderma aculeatus dispersible oil suspension concentrate and Trichoderma aculeatus conidia powder is the same as that in Embodiment 1.

[0033] In this embodiment, the Trichoderma aculeatus dispersible oil suspension preparation has a viable spore rate > 85.73%, a moisture content of 3.2%, a pH value of 5.2, an open flash point of 170°C, 98% of which can pass through a 25 μm test sieve, and a viscosity (20°C) of 0.40 Pas, thermal storage stability (30°C, 2 months) and low temperature stability are qualified. The usage method of Trichoderma aculeatus dispersible oil suspension preparation in this embodiment: adopt ultra-low volume spray technology.

specific Embodiment approach 3

[0034] Specific embodiment three: 12% Trichoderma aculeatus dispersible oil suspension formulation in the present embodiment is as table 3:

[0035] Table 3 Trichoderma aculeatus dispersible oil suspension formulation three

[0036]

[0037] In this embodiment, the preparation method of Trichoderma aculeatus dispersible oil suspension concentrate and Trichoderma aculeatus conidia powder is the same as that in Embodiment 1.

[0038] In this embodiment, the Trichoderma aculeatus dispersible oil suspension preparation has a viable spore rate>86.09%, a moisture content of 3.6%, a pH value of 5.1, an open flash point of 160°C, 98% of which can pass through a 25 μm test sieve, and a viscosity (20°C) of 0.39 Pas, thermal storage stability (30°C, 2 months) and low temperature stability are qualified. The usage method of the Trichoderma aculeatus dispersible oil suspension preparation in this embodiment: dilute the corresponding times when using, and spray.

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Abstract

The invention relates to a dispersible trichoderma asperellum oil suspending agent and a preparation method thereof, belonging to the technical field of a suspending fungicide and aiming at solving the technical problem that existing trichoderma asperellum cannot be stored for a long time. The dispersible trichoderma asperellum oil suspending agent comprises trichoderma asperellum conidia powder, an emulsifier, a stabilizer, a wetting dispersant, an antioxidant, a thickener, an ultraviolet protective agent, a preservative and plant oil. The dispersible trichoderma asperellum oil suspending agent is prepared by the following concrete steps: (I) adding the emulsifier, stabilizer, wetting dispersant, antioxidant, thickener, ultraviolet protective agent and preservative into the plant oil, premixing through a magnetic stirrer, and then processing for 10min-30min in a homogenizer or a high-speed shearing machine to obtain a mixture; and (II) mixing the mixture with the trichoderma asperellum conidia powder at room temperature, and grinding for 10-60 minutes. The dispersible trichoderma asperellum oil suspending agent is less in usage amount, not only is suitable for disease control of tall trees of forests in high mountains with drought, but also has the characteristics of being environmentally friendly, free from resistance, harmless to human and livestock and the like.

Description

technical field [0001] The invention relates to a microbial bactericide and a preparation method thereof, in particular to a trichoderma aculeatus bactericide and a preparation method thereof, and belongs to the technical field of suspension agent bactericides. Background technique [0002] Trichoderma ( Trichoderma. spp) is a filamentous fungus, which is a widely distributed biocontrol fungus in nature and is considered to be one of the most promising biological control factors. At present, there are more than 100 kinds of biological agents with Trichoderma as the main component in the world, and they have successfully prevented and controlled greenhouse plant diseases. Trichoderma has an antagonistic effect on more than 20 pathogenic fungi of 18 genera and a variety of pathogenic bacteria, and has a good control effect on various soil-borne diseases such as white silkworm, Phytophthora, and rice sheath blight. Among them, Trichoderma viride ( Trichoderma viride ), Trich...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/04A01N63/04A01P3/00
Inventor 王志英孙丽丽景天忠曹传旺吴红渠
Owner NORTHEAST FORESTRY UNIVERSITY
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