The invention discloses a tumor vaccine treatment method, which comprises the following steps: patient preparation, preoperative examination, informed agreement,
tumor tissue resection, postoperativetreatment, gp96
protein purification and
clinical treatment. According to an immunization procedure, the patient is subjected to administered subcutaneous or
intradermal injection with a tumor autologous gp96 vaccine, and gp96 interacts with
heat shock protein receptors (CD91, CD36, SR-A, Lox-1, TLR2 and TLR4) on the surface of
antigen presenting cells, the maturation and activation of
antigen-presenting cells can be promoted, which are realized by up-regulating of
cell surface molecules and secreting of cytokines and chemokines, the
adjuvant properties of gp96 and its bonded tumor-derived polypeptides provide an ideal environment for cross-presenting MIHC I and MHC II restricted polypeptides, tumor autologous gp96 activates
tumor cells and micrometastases of tumor
cell-specific CD4<+>
helper T cells and cytotoxic CD8<+> T cells, the method has good immunization characteristic, and the
treatment effect is better than that of the operation and
chemotherapy.