51results about How to "Resist infection" patented technology

An implantable article and method are disclosed for treating pelvic floor disorders such as vaginal vault prolase. A surgical kit useful for performing a surgical procedure such as a sacral colpopexy is also described.

An implantable article and method are disclosed for treating pelvic floor disorders such as vaginal vault prolase. A surgical kit useful for performing a surgical procedure such as a sacral colpopexy is also described.

The invention discloses a soil conditioner for treating saline-alkali soil, and belongs to the technical field of agricultural production. The soil conditioner is prepared by diatomite, attapulgite, halophilic bacteria, humic acid, fulvic acid and biological floras, 300 kilograms of water is added into fungi in the biological floras to prepare an aqueous solution, and the diatomite, the attapulgite and the halophilic bacteria are added into the aqueous solution; then the humic acid and the fulvic acid are mixed to be added into the aqueous solution to be mixed, and therefore particles are obtained. In tillage, the soil conditioner is applied along with bottom fertilization in soil turning, and can promote rapid growth of crops and increase the yield and incomes, and the quality of fruits is remarkably improved.

The invention relates to a bacterial strain for preventing and treating tobacco black shank and the bacteria agent thereof, which is characterized in that: the invention adopts paecilomyces lilacinusZY-19-2, the collection number is CCTCCNO: M207196, as the original bacterial strain to prepare the bacteria agent through a fermentation process, and the fermentation conditions are as follows: 0.8to2.0 percent of colloidal chitin is taken as a carbon source, the initial pH value of the fermentation fluid is 5.5 to 7.0, 0.8 to 1.5 percent peptone is taken as a nitrogen source, 0.1 percent Tween- 80 is taken as a surfactant, the fermentation time is 48 to 72h, the vaccination amount is 0.8 to 2 percent, and the rotational speed of a shaking bed is 100 to 180r / min. The invention has the following advantages that: 1. the paecilomyces lilacinus strain has fast growth, the producing capacity of chitin is strong; 2. the invention can decompose the body walls of flies and maggots and can growon the body walls of flies and maggots or a culture medium which is prepared by the decomposition products of the body walls and water; 3. the invention can be colonized at the root of a tobacco plantforresisting the infection of the tobacco black shank; 4. the invention can effectively overcome the antibacterial effect of the soil and maintain the suppression capacity of the tobacco black shankfora long time.

The invention discloses a high-activity seaweed fertilizer specially used for peanuts and a preparation method thereof and belongs to the technical field of production of crop specially-used fertilizers. The high-activity seaweed fertilizer is prepared from following raw materials comprising: urea, monoammonium phosphate, a calcium magnesium phosphate fertilizer, potassium sulfate, borax, EDTA chelating iron, EDTA chelating manganese, EDTA chelating zinc, ammonium heptamolybdate, a seaweed extraction liquid and seaweed residues. Compared with a fertilizer in the prior art, the high-activity seaweed fertilizer specially used for the peanuts is reasonable in formula, is complete in nutrition, can achieve an effect of increasing yield of the peanuts and improving quality of the peanuts.

A chimeric virus-like granular DNA vaccine able to generate stronger B cell activity, exciting high antibody level, improving cell's immunizing level by cross presentation, and activating T cell, toxic T cell and complete immunoreaction is a recombinant eucaryotic expression carrier of coding gene with chimeric protein. Its preparing process is also disclosed.

The present invention relates to a plant virus inhibitory artificial miRNA (microRNA) and construction and application thereof. The invention firstly designs oligonucleotides with excellent silencing effect according to the nucleic acid sequence of a gene silencing suppressor of a plant virus infecting plants. When the virus infects a plant, these oligonucleotides transfected into the plant can form miRNA in the plant which specially binds to the corresponding parts of mRNA (massager RNA) of the virus gene silencing suppressor to affect the transcription of the mRNA, thereby achieving virus inhibitory effect. In addition, oligonucleotides of the invention are optimized and designed on the basis of locating the most conservative positions after comparing the virus gene silencing suppressors derived from different virus subtypes, the oligonucleotides designed based on these positions has broad-spectrum resistant effect on related viruses after being introduced into the plant.

A device for promoting root function of a plant may include a first container configured to retain a root growing medium; a second container below the first container, wherein the second container is configured to store a liquid; an insulated enclosure surrounding the first container and the second container and including an opening above the first container that is sized so that the plant may grow through the opening; a pump configured to transport the liquid from the second container to the first container; a heater within the insulated enclosure; and a flow path from the first container to the second container that allows the liquid to flow from the first container to the second container after the liquid is transported to the first container.

The invention discloses a lysozyme soil sterilization deep ploughing-free anti-hardening root cultivation and protection microorganism fertilizer. The microorganism fertilizer comprises the following components, by weight, 25-60% of a polyacrylamide, 20-45% of calcium bentonite, 8-22% of biochemical fulvic acid, 1-3% of amino acids, and 2-5% of chelated trace elements, wherein the content of calcium in the calcium bentonite is 13-15%; in the chelated trace elements, the magnesium content is 0.5-0.8%, the zinc content is 0.2-0.8%, the copper content is 0.3-0.8%, the iron content is 0.1-0.2%, and the manganese content is 0.1-0.2%; in the amino acids, the Bacillus megaterium content is 0.05-0.15 billion / g, and the colloidal Bacillus cereus content is 0.05-0.1 billion / g; in the biochemical fulvic acid, the suborder Bacillus content is equal to or greater than 0.2 billion / g, the actinomyce content is equal to or greater than 0.2 billion / g, the Serratia carollera phosphaticum content is equal to or greater than 0.2 billion / g, and the Bacillus mucilaginosus content is equal to or greater than 0.2 billion / g. The microorganism fertilizer has the advantages of simple use, no strict requirements on environments, yield increase guarantee, fertilizer dosage reduction, cost reduction, soil and crop quality improvement, and pollution reduction.

The invention discloses a preparation method for a titanium alloy-hydroxyapatite salt biological ceramic porous material. The preparation method for the titanium alloy-hydroxyapatite salt biological ceramic porous material has the characteristics that the 3D printing technology is applied, and by means of the manner that the proportion value of hydroxyapatite salt powder to titanium alloy matrix powder of all fusion covering layers is dynamically adjusted, the titanium alloy-hydroxyapatite salt biological ceramic porous material with titanium alloy being a central layer and titanium alloy-hydroxyapatite salt being a transition layer is prepared. According to the preparation method for the titanium alloy-hydroxyapatite salt biological ceramic porous material, because the performance advantages of various materials are sufficiently utilized and the internal stress is further reduced, excellent mechanical and biological properties are exhibited, therefore, the preparation method for the titanium alloy-hydroxyapatite salt biological ceramic porous material is suitable for the field of bone tissue replacing or implant repairing.

The invention discloses a shear locus mediated by RNA induced silencing complex and application thereof. The locus can be applied in designing a factitial tiny RNA. In a DNA molecule coding a factitial tiny RNA precursor, the factitial tiny RNA precursor is a single-chain RNA capable of generating a tiny RNA in plant; the single-chain RNA forms a stem-loop structure; a nucleotide sequence containing sequence 1 in a sequence list is arranged in a fragment of the stem part of the stem-loop structure; two base pairs which are not complementary are arranged on the stem part of the stem-loop structure; and the two uncomplementary base pairs correspond to the ninth position and the tenth position from tail end of 5' in the sequence 1. The invention also discloses a method for culturing plant resisting cucumber mosaic virus. With the DNA molecule coding the factitial tiny RNA precursor, the plant with high efficient virus resistance can be obtained, so that the method is a biological engineering method with economy and efficiency.

The invention discloses a method for cultivating anti-cyanosis transgenic cotton and a special expression vector thereof. The method for cultivating the anti-cyanosis transgenic cotton comprises the following steps: introducing the protein-coding genes of an SPGAFP1 protein into a target plant to obtain a transgenic plant with the cyanosis resistance higher than that of the target plant, wherein the amino acid sequence of the SPGAFP1 protein is sequence 2 in a sequence table; the sequence of the protein-coding genes of the SPGAFP1 protein can be sequence 1 in the sequence table; and the target plant and the transgenic plant are dicotyledon, i.e. cotton, preferably the CCRI 24. Experiments prove that the SPGAFP1 cotton obtained by introducing the expression vector into the cotton can effectively resist fungi initiating cyanosis, thus indicating that the existence of signal peptide greatly enhances the disease resistance of the transgenic plant.

The invention discloses a method for preparing a special alga fertilizer for peanuts, belonging to the technical field of production of special fertilizers for crops. The fertilizer is prepared from urea, monoammonium phosphate, a calcium magnesia phosphate fertilizer, potassium sulfate, sodium borate, EDTA chelated iron, EDTA chelated manganese, EDTA chelated zinc, ammonium heptamolybdate, an alga extracting solution and alga residues. Compared with the prior art, the special high-activity alga for peanuts has the advantages of reasonable proportioning and comprehensive nutrients, and can increase the peanut yield and enhance the peanut quality.

The invention discloses an application of GAFP2 protein in cultivating sheath blight-resisting transgenic paddy rice and a special vector. The invention provides an application of GAFP2 protein or an encoding gene thereof or a recombinant vector in regulating and controlling plant disease resistance. The amino acid sequence of a GAFP2 protein is a sequence 2 inside a sequence table or the (29-171)th site amino acid residue of the sequence 2 inside the sequence table and from an N'tail end. Experiments improve that the GAFP2 is inducted into paddy rice so that transgenic paddy rice is obtained, therefore the resistance of paddy rice sheath blight can be effectively enhanced.

The invention discloses a method for cultivating plants resistant to cucumber mosaic virus. The present invention clones the cDNA fragment of the replicase gene of cucumber mosaic virus, which is 308bp long and is located between 1207bp-1515bp of the full-length gene sequence of CMV2a, and then constructs the gene fragment on the RNAi high-throughput expression vector pHellsgate2, and then passes it through Agrobacterium tumefaciens The CMV2aRNAi plant expression vector was introduced into tobacco by the mediated genetic transformation method and stably expressed, and the virus resistance of the transgenic plants was analyzed through experiments, and finally transgenic tobacco plants with enhanced resistance to CMV were obtained; the expression of viral gene fragments in plants was confirmed by experiments The double-stranded RNA can induce the occurrence of RNA interference, and then produce the small interfering RNA of the corresponding gene, and specifically inhibit the infection of the virus.

The invention discloses ralstonia solanacearum N477 extracelluar protein PHD as well as a coding gene thereof and application thereof. An amino acid sequence of the ralstonia solanacearum N477 extracelluar protein PHD is as shown in SEQ ID NO. 1, and a coding gene nucleotide sequence is as shown in SEQ ID NO. 2. The invention further discloses application of PHD or the coding gene thereof to preparation of a biological-source pesticide for resisting bacterial wilt. A primer is designed, genome DNA is taken as a template, a PHD coding region sequence is amplified, a product is connected to a protein expression vector pET30a(+), and is converted into escherichia coli BL21(DE3) for performing protein expression, and PHD protein is purified by a nickel column. According to greenhouse test results, the protein further can induce infection of tobacco mosaic viruses, and the prevention and control effect is 56.88%.

The invention provides a CRISPR / Cas9 system for inhibiting HSV-1 copying and / or target sequence expressing. The system comprises a Cas9 protein and gRNA. The coded sequence of the gRNA comprises one or more of nucleotide sequences as shown in SEQ ID NO.1 to SEQ ID NO.6. The invention also provides a composition and a kit for inhibiting HSV-1 copying and / or target sequence expressing. The Cas9CRISPR / Cas9 system can inhibit HSV-1 virus copying and expression of a target sequence of the HSV-1 virus and can be also applied to treating HSV-1 viral infection or cancer induced by or HSV-1 virus.

The invention relates to the field of pesticides and in particular to an endive leaf spot disease control composition which comprises the following raw materials in parts by weight: 8-12 parts of herba lycopodii, 12-18 parts of radix gentianae macrophyllae, 12-18 parts of herba dianthi, 3-8 parts of rhizoma dioscoreae bulbiferae, 12-18 parts of radix asteris, 2-6 parts of cortex moutan, 12-18 parts of rhizoma chuanxiong, 3-8 parts of rhizoma alismatis, 10-20 parts of herba agrimoniae, 12-18 parts of fructus corni, 8-12 parts of herba geranii, 8-12 parts of terminaliachebuia, 10-20 parts of Chinese actinidia roots, 8-12 parts of perfoliote knotweed herb, 12-18 parts of composite bacteria and 10-20 parts of composite amino acids. By adopting the endive leaf spot disease composition preparedfrom traditional Chinese medicines, growth of pathogenic bacteria is effectively inhibited, endive is not damaged by the composition provided by the invention in the spraying process, meanwhile a goodprevention and treatment function on endive leaf spot diseases is achieved to the maximum extent, a remarkable prevention and treatment effect is achieved, pathogenic bacteria are effectively inhibited and killed, the influence of leaf spot disease pathogenic bacteria and other insects to vegetables is reduced, reproduction of pathogenic bacteria is effectively avoided, and the safety of the vegetables is ensured.

The invention provides a repair method for semi-soil and semi-matrix cultivating soil. The method comprises the following steps: filling the lower part of a cultivating groove with nutritional soil; filling the upper part with a matrix; adding a disinfectant into the cultivating groove uniformly; adding a film to cover the cultivating groove for 7-10 days; uncovering the film to ventilate; detecting residues by means of a germination test; adding a soil reducer and the like to prepare the semi-soil and semi-matrix cultivating soil. The method can repair the matrix effectively, optimizes the physical and chemical characteristics of the matrix, balances nutrients in the matrix and solves the problems of secondary salinization and massive accumulation of soil-borne pathogenic bacteria and thelike. The method can reduce the incidence rate of the soil-borne diseases effectively and promote growth of plants positively.

The invention provides a Phytophthora sojae inhibitor and a preparation method thereof, and relates to the field of plant disease biological prevention and control. According to the invention, the Phytophthora sojae inhibitor comprises propyl gallate, wherein propyl gallate as a food additive is mainly used for the oxidation resistance of food, the value EC50% of propyl gallate to Phytophthora sojae is only 0.458 mmol / L, ie., when the inhibition rate is 50%, the concentration of propyl gallate is only 0.458 mmol / L, so that the safety is high; the inhibition effects on Phytophthora sojae harm mainly comprise that propyl gallate can inhibit the growth, the action mechanism and the target site of Phytophthora sojae, wherein the proteomics technology reveals that the Phytophthora sojae inhibition mechanism is closely related to the great down-regulation of the protein expression in fat transport and metabolism, energy metabolism and carbohydrate metabolism and other key pathways; and the inhibition effects on Phytophthora sojae harm mainly comprise that propyl gallate can stimulate the defense reactions of plants so as to improve the expression quantity of resistance genes, such that the infection of Phytophthora sojae can be resisted.

The invention belongs to the technical field of biological prevention and treatment, and particularly relates to a biocontrol preparation for preventing and treating rice blast, and a preparation method thereof, wherein the biocontrol preparation comprises, by weight, 20-40 parts of gingko endophytic fungus fusarium oxysporum fermentation broth, 10-20 parts of a gingko leaf extract and 5-15 partsof a gingko exopleura extract, and the fermentation broth prepared by solid culture and liquid culture of fusarium oxysporum, the ginkgo leaf extract and the gingko exopleura extract are mixed according to the weight part ratio to obtain the biocontrol preparation. According to the present invention, after the application of the biocontrol preparation, the incidence rate and the disease index of rice blast can be remarkably reduced, the relative control effect exceeds the relative control effect of the conventional chemical control, and the problems of toxicity and residues generated by chemical pesticides can be avoided; and the biocontrol preparation of the present invention is environmentally friendly and efficient, does not easily generate drug resistance, and has a certain applicationprospect.

The present invention relates to the field of pesticides, in particular to a composition for controlling botrytis cinerea of broccoli, which comprises the following raw materials in parts by weight: 10 to 20 parts of callicarpa bodinieri, 12 to 18 parts of gentiana macrophylla, 12 to 18 parts of Herba Pteridis multifidae, 2 to 8 parts of radix semiaquilegiae, 12 to 18 parts of commelina communis,10 to 20 parts of manyleaf paris rhizome, 12 to 18 parts of Belamcanda sinensis, 2 to 8 parts of Flos Eriocauli, 10-20 parts of Chinese holly, 12-18 parts of tobacco, 8-12 parts of fructus piperis longi, 8-12 parts of polygonum cuspidatum, 3-9 parts of fructus trichosanthis, 8-12 parts of reed rhizome, 6-8 parts of complex bacteria, 10-20 parts of complex amino acid, and 8-12 parts of multi-vitamin. The composition of the invention cannot cause harm to the broccoli, and at the same time, the composition has the good prevention and control effect on the botrytis cinerea of broccoli, the incidence of the botrytis cinerea of broccoli is controlled at 1.23%, the total effective rate of the broccoli infected with botrytis cinerea is more than 95%, and the control effect is remarkable.

The invention discloses a method for cultivating plants resistant to cucumber mosaic virus. The present invention clones the cDNA fragment of the replicase gene 1a of cucumber mosaic virus, which is 344bp long, and is located between 1673bp-2016bp of the full-length gene sequence of CMV1a, and constructs this gene fragment on the RNAi high-throughput expression vector pHellsgate2, and then passes The genetic transformation method mediated by Agrobacterium tumefaciens introduces the RNAi plant expression vector into tobacco and stably expresses it, and finally obtains virus-resistant tobacco plants; it is confirmed by experiments that the double-stranded RNA expressing viral gene fragments in plants can induce RNA interference , and then produce the small interfering RNA of the corresponding gene, and specifically inhibit the infection of the virus, and the screened transgenic tobacco has obvious resistance to the infection of cucumber mosaic virus.

The invention discloses a resistance inducer for preventing and treating tobacco weather fleck and a preparation method thereof. The resistance inducer comprises amino-oligosaccharin, potassium oxalate, brassinolide, methyl jasmonate, beta-aminobutyric acid and benzothiadiazole. The resistance inducer prepared in the invention has good prevention and treatment effect on tobacco weather fleck and improves the quality of tobacco leaves without causing pollution to a soil environment.

The invention discloses a gosling pest prevention and antibody treatment method. The method concretely comprises the following steps: diluting a gosling pest live vaccine by using 0.85-0.95% normal saline, and intramuscularly injecting 1mL of the diluted gosling pest live vaccine to every female goose by using a syringe; diluting a goose embryonalisation gosling live vaccine by using the 0.85-0.95% normal saline, and hypodermically injecting 0.1mL of the diluted goose embryonalisation gosling live vaccine to every gosling by using a syringe within 48h after the gosling is hatched; and hypodermically injecting 0.8-1mL of anti-gosling pest serum to the chest of a gosling with the weight being lower than 150g, and hypodermically injecting 1-1.5mL of the anti-gosling pest serum to the chest of a gosling with the weight being greater than 150g. The gosling pest infection of goslings can be systemically prevented and treated through the method.

The invention relates to the field of pesticide and particularly relates to a composition for preventing and treating cabbage chlorotic disorder. The composition comprises, by weight, 10-20 parts by weight of Commelina communis, 12-18 parts by weight of spora lygodii, 12-18 parts by weight of rhizoma acori graminei, 2-8 parts by weight of cornus officinalis, 12-18 parts by weight of leaf of purpleflower holly, 10-20 parts by weight of folium sennae, 12-18 parts by weight of suberect spatholobus stem, 2-8 parts by weight of radix sophorae tonkinensis, 10-20 parts by weight of cortex erythrinae,12-18 parts by weight of pipewort, 8-12 parts by weight of tribulus terrestris, 3-8 parts by weight of lilac daphne flower bud, 8-12 parts by weight of compound bacteria and 8-12 parts by weight of compound amino acids. The composition for preventing and treating cabbage chlorotic disorder effectively inhibits the growth of pathogenic bacteria, does not damage the cabbage in the spraying process,produces the highest effects of preventing and treating cabbage chlorotic disorder, controls the average incidence of cabbage chlorotic disorder to 2.33%, has the total effective rate to the cabbagechlorotic disorder of 95% or more and has significant prevention and treatment effects.

The invention discloses a tussor Yippee gene, and a preparation method and application of a polyclonal antibody thereof. The tussor Yippee gene has the nucleotide sequence disclosed as SEQ ID NO.1 or nucleotide sequence complementary with the sequence disclosed as SEQ ID NO.1. The preparation method of the polyclonal antibody comprises the following steps: carrying out prokaryotic expression on the tussor Yippee gene to obtain a recombinant protein, injecting the recombinant protein into a white rabbit, and collecting the rabbit serum, thereby obtaining the polyclonal antibody. When directly participating in the immune response reaction of tussor as a new Yippee gene, the tussor Yippee gene has the capacity for enhancing tussor autoimmunity, thereby having important meanings in resisting infection of pathogenic microorganisms, enhancing the cocoon fiber yield, reducing the loss and the like. The gene also has important meanings in promoting related researches into other Lepidoptera insects, and can also provide references for controlling Lepidoptera pests.

The invention discloses a broad-spectrum anti-phage E. Coli BL21-(DE3)-PR and application thereof. A recombinant protein expression strain E. coli BL21(DE3)-PR is successfully screened with an engineering strain E. coli E. coli BL21(DE3) as a parent strain. The strain is resistant to infection of a phage capable of splitting E. coli BL21 (DE3). The broad-spectrum anti-phage E. Coli BL21-(DE3)-PR performs growth curve and protein expression assay on the recombinant protein expression strain E. coli BL21(DE3)-PR, and results indicate that the growth capability and protein expression capability of the stain are basically the same as an original strain E. coli BL21 (DE3).

The invention belongs to the technical field of microorganisms, and particularly relates to a composite bacillus agent as well as a preparation method and application thereof. Various bacilli are mixed for secondary fermentation and are mixed with diatomite and fine-pored silica gel; the bacilli such as bacillus subtilis are effective microbial communities; different strains have the synergistic effect; after being applied to soil, the strains rapidly propagate to become dominant bacterial communities, and rhizosphere nutrition and resources of crops are controlled, so that pathogenic bacteriaof continuous cropping, root rot, rhizoctonia rot, gummosis, gray mold and the like lose living space and conditions, cell walls of plant root system cells are thickened, fibrillated and lignified, colloid double-silicon layers are formed, and invasion of the pathogenic bacteria is prevented; the diatomite and the fine-pored silica gel are mixed, so that the soil buffer capacity can be enhanced,water retention and heat preservation are realized, and the drought resistance and cold resistance of crops are enhanced; and meanwhile, leaf protective films can be strengthened, and pathogenic bacteria infection, disease resistance and insect resistance are achieved.

The invention discloses broad-spectrum antiphage E.coli BL21(DE3)-PR and application thereof. The engineering strain escherichia coli E.coli BL21(DE3)-PR is used as a parent strain to successfully screen one recombinant protein expression strain escherichia coli E.coli BL21(DE3)-PR; and the strain can be used for resisting infection of a phage capable of cracking the escherichia coli E.coli BL21(DE3)-PR. The recombinant protein expression strain escherichia coli E.coli BL21(DE3)-PR is subjected to growth curve and protein expression determination; and a result shows that the growth capabilityand protein expression capability of the strain are basically the same as those of an original strain E.coli BL21(DE3).