Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

CRISPR/Cas9 system, method and kit for inhibiting HSV-1 copying and/or target sequence expressing and application thereof

A technology of HSV-1 and target sequence, applied in the field of CRISPR/Cas9 system

Inactive Publication Date: 2019-03-15
SUN YAT SEN UNIV
View PDF15 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the past two years, the research community has begun to try to apply the Cas9CRISPR / Cas9 system to inhibit HSV-1 replication and / or target sequence expression. However, since the actual application effect of gRNA is affected by many factors, it can effectively inhibit HSV-1 from the viral genome level. Not many gRNAs are reported for proliferation and replication

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • CRISPR/Cas9 system, method and kit for inhibiting HSV-1 copying and/or target sequence expressing and application thereof
  • CRISPR/Cas9 system, method and kit for inhibiting HSV-1 copying and/or target sequence expressing and application thereof
  • CRISPR/Cas9 system, method and kit for inhibiting HSV-1 copying and/or target sequence expressing and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0106] The following descriptions are preferred implementations of the embodiments of the present invention. It should be pointed out that those skilled in the art can make some improvements and modifications without departing from the principles of the embodiments of the present invention. These improvements And retouching are also regarded as the scope of protection of the embodiments of the present invention.

[0107] The present disclosure is not limited to the specific details of the construction methods or steps of the recombinant lentiviral vectors or other components set forth herein. In light of the disclosure, it will be apparent to those skilled in the art that the compositions and methods disclosed herein can be prepared, practiced, used, carried out and / or formed in various ways.

[0108] The phraseology and terminology used herein are for the purpose of description only and should not be taken as limitations on the scope of the claims. As used in the specificati...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a CRISPR / Cas9 system for inhibiting HSV-1 copying and / or target sequence expressing. The system comprises a Cas9 protein and gRNA. The coded sequence of the gRNA comprises one or more of nucleotide sequences as shown in SEQ ID NO.1 to SEQ ID NO.6. The invention also provides a composition and a kit for inhibiting HSV-1 copying and / or target sequence expressing. The Cas9CRISPR / Cas9 system can inhibit HSV-1 virus copying and expression of a target sequence of the HSV-1 virus and can be also applied to treating HSV-1 viral infection or cancer induced by or HSV-1 virus.

Description

technical field [0001] The invention relates to the field of gene therapy, and relates to a CRISPR / Cas9 system, method, kit and application for inhibiting HSV-1 replication and / or target sequence expression. Background technique [0002] Herpes simplex virus (HSV) is an enveloped double-stranded DNA virus. The viral genome contains 74 genes related to virus proliferation and infection. It can be divided into two serotypes, HSV-1 and HSV-2. HSV-1 can be transmitted among the population, and one-third of the infected population will have recurrent infection. Herpes simplex keratitis is the leading cause of corneal blindness, and approximately 1.5 million people around the world suffer from herpes simplex keratitis every year. [0003] After the primary infection of HSV-1, the virus may be dormant in the nerve cells in the body, and the reactivation of the virus will cause the secondary infection of herpes simplex virus. In secondary infection, the latent virus is transported...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N5/10C12N15/90A61K48/00A61P31/22
CPCA61K48/0008A61K48/0058C12N15/113C12N15/907C12N2310/10C12N2510/00
Inventor 黄军就松阳洲陈昱僖支胜尧梁普平
Owner SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com