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Tussor Yippee gene, and preparation method and application of polyclonal antibody thereof

A technology of polyclonal antibody and tussah silkworm, which is applied in the field of genetic engineering to achieve the effects of reducing losses, improving immunity, and increasing silk production

Inactive Publication Date: 2015-12-23
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there are few reports on the immune mechanism of tussah silkworms. The diseases caused by these pathogens have brought great economic losses to sericulture farmers every year. Therefore, it will be very meaningful to improve the production of tussah silkworms by improving their own immune function. work

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  • Tussor Yippee gene, and preparation method and application of polyclonal antibody thereof
  • Tussor Yippee gene, and preparation method and application of polyclonal antibody thereof
  • Tussor Yippee gene, and preparation method and application of polyclonal antibody thereof

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Embodiment 1

[0021] 1. Materials

[0022] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.

[0023] 2. Method

[0024] 2.1 Acquisition of Tussah mori Yippee gene

[0025] Tussah silkworm total RNA was extracted by Trizol method, and tussah silkworm cDNA was obtained by reverse transcription using the tussah silkworm total RNA as a template. According to the tussah silkworm Yippee gene sequence shown in SEQIDNO.1, design specific amplification primers as follows, wherein, the upstream primer F1 contains the XhoI restriction site, and the downstream primer R1 contains the BamHI restriction site:

[0026] SEQ ID NO.2: Upstream primer F1: CGC CTCGAG GTATACTAGGTTAAC

[0027] SEQ ID NO.3: downstream primer R1: GGC GGATCC ATGGGAAGAATATTTT

[0028] Using tussah silkworm cDNA as a template, using the above-mentio...

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Abstract

The invention discloses a tussor Yippee gene, and a preparation method and application of a polyclonal antibody thereof. The tussor Yippee gene has the nucleotide sequence disclosed as SEQ ID NO.1 or nucleotide sequence complementary with the sequence disclosed as SEQ ID NO.1. The preparation method of the polyclonal antibody comprises the following steps: carrying out prokaryotic expression on the tussor Yippee gene to obtain a recombinant protein, injecting the recombinant protein into a white rabbit, and collecting the rabbit serum, thereby obtaining the polyclonal antibody. When directly participating in the immune response reaction of tussor as a new Yippee gene, the tussor Yippee gene has the capacity for enhancing tussor autoimmunity, thereby having important meanings in resisting infection of pathogenic microorganisms, enhancing the cocoon fiber yield, reducing the loss and the like. The gene also has important meanings in promoting related researches into other Lepidoptera insects, and can also provide references for controlling Lepidoptera pests.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a preparation method and application of tussah silkworm Yippee gene and its polyclonal antibody. Background technique [0002] Tussah silkworms belong to the wild silk insects of Lepidoptera, Sagittaridae, and Tussah genus. Its cocoon silk production is second only to silkworms. The main distribution areas are China, India and South Korea. Its main value is reflected in its use as raw materials for the textile industry. Moreover, it has high economic value such as medicine and food, and can be used to supplement the amino acids necessary for the human body. It has the advantages of large-scale breeding and large individuals, and can be used as a bioreactor to produce medical drugs and useful proteins. Therefore, the study of its functional genes is of great significance for the protection and development of gene resources of tussah mori. [0003] At present, importan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C07K16/18C12N15/70C12R1/19
Inventor 刘朝良孙钰朱保建王磊魏国清钱岑戴立上孙誉轩
Owner ANHUI AGRICULTURAL UNIVERSITY
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