61 results about "White rabbit" patented technology

The invention relates to a clothianidin antigen, an antibody and an application thereof, and belongs to the technical field of immunochemistry analysis. The clothianidin antigen and the antibody of the invention are specially used for clothianidin specific polyclonal antibody preparation, enzyme-linked immunoassay (ELISA), and high-sensitivity and rapid detection of clothianidin residues in environment and agricultural products. A hapten is synthesized by substituting a chlorine atom on a thiazole ring of clothianidin, has a chemical name of 3-(5-((3-methyl-2-nitroguanidine)-yl methyl)thiazole-2-mercapto)propionic acid, and is coupled with bovine serum albumin and ovalbumin to prepare an antigen and an envelope antigen. Newzealand white rabbit is immunized by the immunizing antigen to obtain the specific polyclonal antibody of clothianidin. The established ELISA linear scope is 1.1 microgram/L-2 mg/L, and the detection limit is 1.1 microgram/L. The hapten synthetic technology of the invention is simple and feasible, high in antibody specificity, and suitable for detection and on-site monitoring of mass samples in environment and agricultural products.

The invention relates to a breeding method of colourful rabbits. The method comprises the following steps of: (1) breeding a pure breeding colourful rabbit for four generations to generate special strains, and selecting a colour male rabbit from the special strains; (2) selecting a female rabbit from white rabbits; and (3) crossbreeding the selected colour male rabbit and female rabbit to generate offspring which have the hair colour of the colourful rabbits, and thus obtaining the colourful rabbits which have the hair colour of the colourful rabbits and uniform hair colour. In the method, the colourful rabbit is crossbred with the white rabbit with excellent production property and the colourful hair colour gene of the rabbit is dominant to the white gene, so the hair colour of the generated offspring accords with that of the colourful rabbit and is uniform. At the same time, because white rabbit has excellent production property, the rabbits with high-quality skin can be generated, the meat production property due to hybrid advantages can be improved and the added value of the rabbit skin is improved.

The invention belongs to the technical field of culture and particularly relates to a method for breeding a new local stress-resistant meat rabbit species. According to the method, Sichuan white rabbits and German synthetic white rabbits are taken as breeding materials, and the new local stress-resistant meat rabbit species is bred through a grading hybridization method. The new meat rabbit species bred through the method has the advantages of the Sichuan white rabbits and the advantages of the German synthetic white rabbits and is strong in stress resistance, and the fattening survival rate reaches over 98%; bucks are strong in sexual desire and high in semen quality; does are obvious in estrus and easy in mating, the blood-nest mating pregnancy ratio reaches up to 82%, the number of annual production births is over 7-8 under the condition of artificially-assisted mating, the continuous production performance is good, and the new meat rabbit species has very strong adaptability to the climate environment and culture characteristics of the southwest of China.

The invention discloses a shredded roasted rabbit product and a making method thereof. The roasted rabbit product is prepared by adding accessories such as salt, white granulated sugar, a water retention agent, monosodium glutamate, D-sodium ascorbate, dark soy sauce, cooking wine, composite seasoning spice and condiments into a white rabbit material, and kneading and roasting the rabbit material. The preparation method comprises the following steps of: selecting the whole white rabbit material which passes quarantine inspection and is subjected to finishing process, adding the accessories into the rabbit, kneading the rabbit in vacuum, roasting and finishing the rabbit, packing the rabbit in vacuum, and sterilizing the rabbit at a high temperature. The roasted rabbit product is unique in flavor, pure in mouthfeel, bright in color, easy to preserve and convenient to carry and eat, and people do not feel greasy even when eating the rabbit frequently; and the preparation method is short in flow, easy to operate and suitable for industrialized production.

The invention relates to a method for breeding a blue-eye white meat rabbit, belonging to the technical field of rabbit breeding. The invention discloses a method for breeding a blue-eye white meat rabbit variety. The blue-eye white meat rabbit variety is bred by using a Yufeng yellow rabbit and a Harbin white rabbit as hybrid parents through methods of hybridization, backcross and the like. The method has the advantages of short time and high speed; the new bred meat rabbit variety keeps the body type of the Harbin white rabbit, has white hair all over the body, common hair type, deep blue eye balls, long back waist, plump rear quarters and strong limbs, and has the advantages of high protophase growth speed, high feed utilization rate, strong lactation capacity, strong premonition and the like; and the blue-eye white meat rabbit is extremely different from other white meat rabbit varieties in the aspect of the special blue eye characteristics.

An object of the invention is to provide a transdermal absorption preparation capable of sustaining the blood morphine concentration at an effective level over at least 48 hours, etc. This transdermal absorption preparation comprises a medicinal composition for transdermal absorption in which an active ingredient selected from morphine and salts thereof is blended in such an amount as corresponding to the saturation solubility or more in an active ingredient-holding vehicle having fluidity at a temperature around the human skin surface temperature and at least a portion of the active ingredient is held in a crystalline form, characterized in that, in the case where a preparation obtained from the above-described medicinal composition for transdermal absorption is applied to the uninjured skin of the back of a white rabbit having been shaven with electrical clippers for 72 hours, the available amount of the active ingredient per single dose of the preparation is from 10 mg to 400 mg in terms of morphine base, and the plasma concentrations of the active ingredient 24 hours and 48 hours after the application of the preparation under the above-described conditions are each at least 40 ng/mL in terms of morphine base, and so on.

The invention provides a bulleyaconitine fat emulsion transfusion and its producing method, including effective component, medicinal auxiliary and emulsifier, and its characteristic: it uses bulleyaconitine as the effective component, contains 0.1-0.8mg of bulleyaconitine per bottle, and mixes in a proper amount of medicinal auxiliary and emulsifier to prepare the medicinally acceptable fat emulsion. At normal temperature, the emulsion can be placed for 1 year, stable and reliable, and through stimulative test on big white rabbits, the stimulative property of the emulsion is grade 0-1 and has better antiphlogistic and analgesic effect and energy supplementing effect and simultaneously, quickly takes effect and has good compliance of patient.

The invention discloses a time synchronization precision detection system. The invention relates to a time synchronization precision detection system. The objective of the invention is to solve the problem that an external detection device of an existing imaging unit outputs no initial signal at an exposure moment. According to the method, timestamp information cannot be compared, meanwhile, the problems of delay output by an initial signal at the exposure moment from the interior of an imaging unit, delay sent into external detection equipment and the like need to be tested, and the method comprises a bottom table unit, a time synchronization detection unit, an image acquisition unit and n groups of CMOS imaging units; Time stamp information is generated in the imaging unit, and the timestamp information does not need to be transmitted back and forth; Based on a simplified White rabbit protocol, the time synchronization precision is detected by comparing the timestamp information ofthe crystal with higher frequency stability.

The invention relates to livestock breeding field, and more specifically relates to a long hair rabbit feed preparation method, the Chinese white rabbit feed prepared by the formulation is low in pollution rate, protein and vitamin contents in the feed are rich, various nutrition component collocation is reasonable, and mulberry leaf is added into the formulation, so that the feed contains vegetable protein, the feed is easier to digest in the consumption of a long hair rabbit, and the long hair rabbit is more healthy to grow.

Provided is a dedicated walking-type mechanical rabbit for education of science popularization for teenagers. The dedicated walking-type mechanical rabbit is mostly composed of a rabbit body main trunk frame, a head model component, a built-in simulated left-eye miniature camera, a left movable-type pitch-adjusting eye socket, a simulated rabbit sound buzzer rabbit mouth device, a stimulated rabbit beard built-in remote-control antenna module, a built-in simulated right-eye miniature camera, a right movable-type pitch-adjusting eye socket, a trunk telescopic support spring, a rabbit body auxiliary trunk frame, a back left-leg linkage component, a back right-left linkage component, a back right cross-bar linkage component, a back left cross-bar linkage component, a back left-foot pulley assembly, a back left pulley, a front left-leg linkage component, a front-back leg linkage component, a front left cross-bar linkage component, a front left-foot pulley assembly, a front right-foot pulley assembly, a front left pulley, a front-back cross-bar linkage component, a front right pulley, a combined-type transmission mechanism with a storage battery and a white rabbit modeling lint coat. The dedicated walking-type mechanical rabbit for education of science popularization for teenagers, as a teaching aid especially for teenagers and children, also serves as a perfect helper of people engaged in scientific research and animal observation organizations for exploration of motion trails of rare animals in old-growth forests.

The invention discloses a preparation method of postpartum white rabbit feed. The feed comprises sweet potato powder, almond powder, cabbage powder, cucumber powder, carrot meal, pumpkin powder, green vegetable powder, chestnut powder, apple powder, banana meal, strawberry powder, bean dreg powder, capsella bursa-pastoris powder, bread powder, plantain powder, dandelion powder and radix isatidis powder, and the percentage adopts weight percentage. The feed has the advantages that the cost is low, the charge mixture is easy to obtain, formulation, compatibility and the preparation method are simple, the feeding efficiency is good, white rabbits fed are fast to grow and develop, big and healthy, have beautiful bodily forms and bright coat color, the disease incidence of the white rabbit is extra-low, and the feed effectively solves the defects that the white rabbits are slow to grow and develop, small and light, the coat color is not thick, dense, bright and smooth, the hair is loose and scattered, and the white rabbits do not like move and are easy to sicken because of nutritive deficiency of white rabbits when certain raisers, plants and large-scale raising plants feed the white rabbits.

The invention discloses a hemostatic antibacterial healing-promoting medical dressing containing lamiophlomis rotata iridoid glycoside active ingredient composite medical ACF and belongs to the technical field of preparation of medical dressings. The medical dressing is composed of the following components in percentage by weight: 1-20% of lamiophlomis rotata iridoid glycosides, 0.01-1% of hexadecyl trimethyl ammonium chloride, 1-5% of polyvinyl alcohol or collagen and the balance of a medical ACF matrix. The lamiophlomis rotata iridoid glycoside active ingredients and polyvinyl alcohol or collagen and the like are combined to be prepared into a solution dosage form and a gel dosage form, and the dosage forms are compounded with medical ACFs so as to prepare a series of external medical dressings. By utilizing the strong effects of the ACF for adsorbing wound exudates, activating a clotting mechanism and accelerating hemostasis, the 'synergistic effect' between the two is achieved, and the medical dressing can be used for treating infectious wounds, surgical trauma bleeding and the like, has excellent hemostatic antibacterial healing-promoting effects and also has wide application prospects. The medical dressing disclosed by the invention is reasonable in formula and convenient in preparation and has the effects of controlling the wound infection and promoting healing of the wound. The hemostasis models for wounds at artery and ventral aorta in ears of large-ear white rabbits and human-mouse livers prove that the effective rate reaches 95% or higher.

The invention discloses a pefloxacin coupling compound with general formula (I), which comprises coupling compounds of pefloxacin hapten and bovine serum albumin of carrier substance which can produce immunogen or egg albumin. Thereinto, n stands for molecular number of pefloxacin combined with a bovine serum albumin molecule, the said n is an integer between one and twenty, and BSA stands for bovine serum albumin with 6.6KDa-6.9KDa of molecular weight. The invention also discloses a process for preparing the said coupling compound, which consists of joining pefloxacin and carrier substance which can produce immunogen to obtain the said coupling compound which induces immune system of animals to produce antibody. By immuning white rabbits from New Zealand, the pefloxacin coupling compound of this invention has prepared antiserum with 1:512,000 of potency, of which the lowest check limit is 0.01ppb. The invention is characterized in that it is simple, rapid, specific, exact and so on, which provides a foundation for preparing enzyme immunoassay agent box of pefloxacin.

The invention discloses rabbit anti-Hematodinium sp serum, which is rabbit antiserum which is obtained by first using Hematodinium sp polypide-containing body fluid of an infected crab as an antigen, then processing the antigen to obtain an immunizing antigen which is a Hematodinium sp polypide suspension and finally vaccinating a white rabbit. The invention also discloses application of the rabbit antiserum to a method for indirect fluorescent antibody detection, which is implemented by dripping the rabbit antiserum into a sample to be detected for primary antibody incubation, dripping isosulfocyanic acid fluorescent labeled sheep and rabbit antibodies for secondary antibody incubation and performing microscopic examination after mounting processing. The rabbit antiserum for detecting Hematodinium sp of the invention has strong specificity, high sensitivity and short detection time, and can be used for diagnosing the potential infection and early infection with the Hematodinium sp. The detection method of the invention is simple in operation and performs qualitative and quantitative detection on whether sea food shellfish is infected with the Hematodinium sp.

The invention provides an external patch for treating mammitis and breast hyperplasia and relieving breast tumor pain and a preparation method of the external patch for external use. Pure natural Chinese medicinal herbs such as nutgrass galingale rhizome, clematis root, turmeric root-tuber, dahurian angelica root, Szechuan lovage rhizome and Chinese angelica are selected as raw materials. The external patch has the effects of promoting blood circulation and removing blood stasis, reducing swelling and resolving mass, and regulating the flow of Qi to alleviate pain; the obtained patch is convenient to use, and effectively and quickly takes effect on the focus without toxic and side effects. Toxicity test results indicate that acute dermal toxicity of white rats is at the actually nontoxic class and acute skin irritation intensity of white rabbits is nonirritant, and therefore, the external patch completely conforms to the national related health standards and is very safe to use.

The invention discloses a method of enzymoimmunoassay for detecting deltamethrin polyclonal antibody and the application thereof. The method is used for obtaining the polyclonal antibody by using healthy white rabbits immunized by a conjugate (DM -BSA) of deltamethrin hapten (DM) and bovine serum albumin (BSA); the polyclonal antibody is filtered, by taking the deltamethrin as a standard product and the conjugate (DM -OVA) of DM and ovalbumin (OVA) as envelope antigen, an indirectly competitive enzyme-linked immunization for deltamethrin pesticide is established in food. The method of the invention creates an indirect ELISA method for deltamethrin pesticide in food, thus providing a rapid and high-efficiency detecting means for detecting the residues of deltamethrin in food. The method of the invention is low in cost and good in stableness and repeatability due to the adoption of the polyclonal antibody. The sensitivity is 0.110 microgram mL to 0.237 microgram mL <-1>; linear range is 0.015 microgram mL to 100 microgram mL <-1>. The preliminary treatment of a sample is simple.

The anti-thymosin pro-alpha polyclonal antibody and its preparation method and application relate to an anti-thymosin pro-alpha polyclonal antibody. The anti-prothymosin α polyclonal antibody is a polyclonal anti-GST-ProTα antiserum obtained after immunizing New Zealand white rabbits with a fusion protein prepared by using GST-ProTα fusion expression. First prepare the fusion protein GST-ProTα, and then prepare the antibody. It can be used for immunoblotting and ELISA immunoassay of various samples and cells containing ProTα components, and tissue endogenous ProTα. Using the antibody to detect the ProTα level in urine can be used to diagnose urinary system tumors such as bladder cancer, kidney cancer and prostate cancer. The antibody has high titer and strong specificity. Various samples containing ProTα can be detected. Using prothymosin α in urine as a tumor marker for the diagnosis of urinary system cancer. The total process does not exceed 4 hours, and the required amount of samples is small.

The invention discloses a construction method of an animal model of the child and adolescent metabolic syndrome. A phased feeding mode is used, common maintenance feed, high-fat high-cholesterol feedand forage are used, and white rabbits which are 6-8 weeks old (child stage) are bred, so that the animal experiment model of the child and adolescent metabolic syndrome is successfully built. The model simultaneously has symptoms of early coronary heart disease, atherosclerosis, fatty liver and non-alcoholic fatty hepatitis and liver fibrosis, proteinuria nephrosis and the like caused by the metabolic syndrome, and can be used for etiological and pathological research and pharmaceutical evaluation of the metabolic syndrome and concurrent multi-organ lesions of the metabolic syndrome.

The invention discloses a lomefloxacin coupling compound with general formula (I), which comprises coupling compounds of lomefloxacin hapten and bovine serum albumin of carrier substance which can produce immunogen or egg albumin. Thereinto, n stands for molecular number of lomefloxacin combined with a bovine serum albumin molecule, the said n is an integer between one and twenty, and BSA stands for bovine serum albumin with 6.6KDa-6.9KDa of molecular weight. The invention also discloses a process for preparing the said coupling compound, which consists of joining lomefloxacin and carrier substance which can produce immunogen to obtain the said coupling compound which induces immune system of animals to produce antibody. By immuning white rabbits from New Zealand, the lomefloxacin coupling compound of this invention has prepared antiserum with 1:6400 of potency, of which the lowest check limit is 1ppb. The invention is characterized in that it is simple, rapid, specific, exact and so on, which provides a foundation for preparing enzyme immunoassay agent box of lomefloxacin.

The invention discloses a process for preparing specific antibody for PCDH10 C end albumen fragment molecule and. The method comprises, using polymerase chain reaction, directly cloning PCDH10C 3'end base sequence to the prokaryotic cell expression system from vitro cultured cell strain to purify the PCDH10 C end amalgamation albumen; using purified PCDH10 C end amalgamation albumen as antigen immunity white rabbit, producing anti PCDH10 C end antibody; collecting antiserum to obtain anti PCDH10 C end albumen specific antibody of rabbit with high-purity and high activity after affinity purification. Specific antibody for PCDH10 C end albumen fragment molecule prepared in the invention is one of the main instrumentalities for researching PCD10 mediated signal pathway transduction. The epitope of specific antibody for PCDH10 C end albumen fragment molecule is a potential target of molecular targeted therapy. The specific antibody for PCDH10 C end albumen fragment molecule can be used for clinically estimating the change of tumor gene expression and metastatic potential.

A garbage deodorant is prepared from substances in parts by mass as follows: 10-20 parts of a plant hydrated extract, 30-50 parts of edible mushroom waste and 5-10 parts of a microbial agent. The garbage deodorant has an effective application in the aspect of deodorization of stale refuse or domestic organic garbage. The plant deodorant product is toxic-free and harmless and has no irritation. Experiments through laboratory rats and little white rabbits prove that the deodorant has no transoral toxicity or irritation to skin. The efficient compound microbial deodorant for a garbage dump is lowin cost, high in efficiency, wide in application and very practical in specific production.

The invention relates to a dyeing process of a rabbit hair product. The dyeing process comprises the following steps of (1) dyeing white or gray-white rabbit hair into black, frequently stirring in a soaking process, wringing the water content in the hair after taking out, then putting into a solution composed of 10 gram/liter of sulphurous acid and 10 to 20 gram/liter of salt, wherein the temperature of the solution is kept at 28 DEG C to 35 DEG C, soaking for 8 to 10 hours, and frequently stirred; (2) flushing the soaked hair at room temperature with clear water for 15 to 20 minutes, and immediately putting into a dye vat after wringing out; (3) washing in 1 to 15 gram/liter of cleaning powder water solution, wherein the water temperature is kept at 35 DEG C, continuously stirring, and then soaking in clear water for 30 minutes. The total processing time is 25 hours, the hair is modified after being dyed, and the hair is kneaded to be soft and is combed after being aired. The method is favorable for scientifically preparing and producing the rabbit hair in an environment-friendly manner, people can conveniently use, and the quality of the prepared rabbit hair is good.