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Biocontrol preparation for preventing and treating rice blast, and preparation method thereof

A biocontrol agent and rice blast technology, applied in botany equipment and methods, biocide, biocide, etc., can solve the problem of cultivation management that is difficult to form a systematic and standardized technical system, difficult to achieve ideal results, and ecological environment. and human health hazards

Pending Publication Date: 2019-12-13
HUNAN UNIV OF SCI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Breeding disease-resistant varieties is an economical and environmentally friendly way, but due to the complex genetic background of the blast fungus, which is prone to mutation, coupled with the simplification of blast-resistant varieties, and the genetic homogeneity of the main varieties, it is difficult for disease-resistant varieties to keep up. The mutation rate of the physiological race of the pathogenic blast fungus often leads to a new rice variety with blast resistance losing resistance after a few years of large-scale planting and becoming a susceptible variety
Cultivation management is difficult to form a systematic and standardized technical system, so it is difficult to achieve the desired effect in actual production
In order to meet the needs of production, chemical control is a common means of controlling rice blast, but due to the unreasonable use of chemical agents, the resistance of blast fungus to several main fungicides commonly used in production (cyclazole, isomydazole) is gradually increasing , making its control efficiency decline continuously, and has brought serious harm to the ecological environment and human health that cannot be ignored

Method used

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  • Biocontrol preparation for preventing and treating rice blast, and preparation method thereof
  • Biocontrol preparation for preventing and treating rice blast, and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] (1) Inoculate the endophytic fungus Fusarium oxysporum of Ginkgo biloba on the PDA solid slant medium and cultivate it for 24-48h. When the strain grows vigorously, pick the mycelium and inoculate it in the PDB liquid medium, and place it in a constant temperature shaking incubator for 26-28h. ℃, 180r / min fermentation and cultivation for 7 days to obtain a Fusarium oxysporum fermentation liquid, the concentration of Fusarium oxysporum in the fermentation liquid was ≥ 1×10 7 cfu / ml;

[0026] (2) Ginkgo leaves are dried and pulverized through a 40-mesh sieve, then put into an extraction tank, add 4 times of Ginkgo leaves weight simultaneously, and the volume concentration is 60% ethanol to extract 2 times, extract 70min each time, and combine the extracts; Distill the extract under reduced pressure to 1 / 5 of the original volume to obtain a concentrated solution, add an equal volume of purified water to the concentrated solution, precipitate for 12-15 hours, and take the s...

Embodiment 2

[0030] (1) Inoculate the endophytic fungus Fusarium oxysporum of Ginkgo biloba on the PDA solid slant medium and cultivate it for 24-48h. When the strain grows vigorously, pick the mycelium and inoculate it in the PDB liquid medium, and place it in a constant temperature shaking incubator for 26-28h. ℃, 200r / min fermentation and cultivation for 5 days to obtain a Fusarium oxysporum fermentation broth, the concentration of Fusarium oxysporum in the fermentation broth was ≥ 1×10 7 cfu / ml;

[0031] (2) Ginkgo biloba is dried and pulverized through a 50-mesh sieve, then put into an extraction tank, while adding 6 times of Ginkgo biloba weight, volume concentration is 80% ethanol extraction 3 times, each extraction 60min, merging extract; Distill the extract under reduced pressure to 1 / 10 of the original volume to obtain a concentrated solution, add an equal volume of purified water to the concentrated solution, precipitate for 12-15 hours, and take the supernatant; dry the superna...

Embodiment 3

[0035] (1) Inoculate the endophytic fungus Fusarium oxysporum of Ginkgo biloba on the PDA solid slant medium and cultivate it for 24-48h. When the strain grows vigorously, pick the mycelium and inoculate it in the PDB liquid medium, and place it in a constant temperature shaking incubator for 26-28h. ℃, 190r / min fermentation and cultivation for 6 days to obtain a Fusarium oxysporum fermentation broth, the concentration of Fusarium oxysporum in the fermentation broth was ≥ 1×10 7 cfu / ml;

[0036] (2) Ginkgo leaves are dried and pulverized through a 60-mesh sieve, then put into an extraction tank, add 8 times of Ginkgo leaves weight simultaneously, and the volume concentration is 70% ethanol to extract 2 times, extract 70min each time, and combine the extracts; Distill the extract under reduced pressure to 1 / 8 of the original volume to obtain a concentrated solution, add an equal volume of purified water to the concentrated solution, precipitate for 12-15 hours, and take the sup...

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Abstract

The invention belongs to the technical field of biological prevention and treatment, and particularly relates to a biocontrol preparation for preventing and treating rice blast, and a preparation method thereof, wherein the biocontrol preparation comprises, by weight, 20-40 parts of gingko endophytic fungus fusarium oxysporum fermentation broth, 10-20 parts of a gingko leaf extract and 5-15 partsof a gingko exopleura extract, and the fermentation broth prepared by solid culture and liquid culture of fusarium oxysporum, the ginkgo leaf extract and the gingko exopleura extract are mixed according to the weight part ratio to obtain the biocontrol preparation. According to the present invention, after the application of the biocontrol preparation, the incidence rate and the disease index of rice blast can be remarkably reduced, the relative control effect exceeds the relative control effect of the conventional chemical control, and the problems of toxicity and residues generated by chemical pesticides can be avoided; and the biocontrol preparation of the present invention is environmentally friendly and efficient, does not easily generate drug resistance, and has a certain applicationprospect.

Description

technical field [0001] The invention belongs to the technical field of biological control, and in particular relates to a biological control preparation for controlling rice blast and a preparation method thereof. Background technique [0002] Rice is one of the most important food crops in the world, and more than half of the world's population feeds on rice. Rice planting area accounts for about 20% of the world's total grain planting area. Rice blast (the pathogen is Magnaporthe oryzae, and its asexual generation is Pyiicularia oryzae) is one of the most serious rice diseases in the world. Rice-growing countries cannot avoid the losses caused by rice blast, which not only reduces the yield of rice, but also has a great impact on the quality of rice. Plant disease experts estimate that at present our country directly loses 3 million tons of rice every year due to rice blast. [0003] At present, the control measures of rice blast mainly include breeding of resistant var...

Claims

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Application Information

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IPC IPC(8): A01N65/00A01N63/04A01N37/40A01N43/16A01P3/00
CPCA01N37/40A01N43/16A01N65/00
Inventor 何福林袁志辉张斌刘小文张祖姣
Owner HUNAN UNIV OF SCI & ENG
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