Bacterial strain for preventing and controlling tobacco black shank and bacterium agent thereof

A technology of tobacco black shank and bacterial agent, applied in the field of microorganisms, can solve the problems of tobacco production loss, etc., and achieve the effects of overcoming the antibacterial effect, strong chitinase production ability, and fast growth

Active Publication Date: 2010-04-14
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the survey of major smoking areas in 16 provinces and cities across the country from 1989 to 1991, except for Heilongjiang, which has not yet occurred, other smoking areas have occurred, and Henan, Shandong, Anhui, Yunnan and other provinces have occurred more frequently, with a general incidence rate of 5%- 15%, and more than 30% in severe cases, causing great losses to tobacco production

Method used

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  • Bacterial strain for preventing and controlling tobacco black shank and bacterium agent thereof
  • Bacterial strain for preventing and controlling tobacco black shank and bacterium agent thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Example 1 Liquid formulation

[0016] The original species of Paecilomyces lilacinus (ZY-19-2) cultivated in a test tube is cultivated according to the three-stage process of triangular shake flask → seed tank → fermentation tank. In a 50L fermenter, filled with 30L of 2% colloidal chitin liquid medium, the initial pH of the fermentation broth was 6.5, 1.0% peptone as the nitrogen source, 0.1% Tween 80 as the surfactant, and the temperature was 0.05mpa. Sterilize for 30 minutes, after cooling to room temperature, insert 2.0L of strain ZY-19-2 liquid seeds, culture temperature 28℃, ventilation volume 1.5M 2 / h, the stirring speed is 120r / min, after 48 hours, the culture solution can be directly used as a liquid fungicide for field production.

[0017] Method of using Paecilomyces lilacinus (ZY-19-2) liquid fungicide: Dosage is 50L / mu, in Fenchen Township, Xiang County, apply the pesticide four times to tobacco plants in the field period that had black shank disease: First Th...

Embodiment 2

[0018] Example 2 Solid formulation

[0019] Diatomaceous earth and peat are used to prepare the carrier adsorbent for the fermentation broth. The preparation method of the carrier adsorbent is to use 1 kg of diatomaceous earth (60%) and peat (40%) as the carrier per liter of culture broth. The amount of spores will be 2×10 after natural drying. 8 A / g solid sterilization preparation, then packaged into a product for field production.

[0020] How to use the solid fungicide of Paecilomyces lilacinus (ZY-19-2): 1. Before the tobacco seedlings are transplanted, the solid fungicides are sprayed in the planting holes, and 15g of solid fungicides are applied to each hole, and then the tobacco seedlings are transplanted Planted in the hole for colonization. 2. Before the occurrence of black shank disease during transplanting of tobacco seedlings, a solid fungicide (15g) was sowed on the soil surface at the root of the tobacco stem, and 300 mL of clean water was poured.

[0021] The results...

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Abstract

The invention relates to a bacterial strain for preventing and treating tobacco black shank and the bacteria agent thereof, which is characterized in that: the invention adopts paecilomyces lilacinusZY-19-2, the collection number is CCTCCNO: M207196, as the original bacterial strain to prepare the bacteria agent through a fermentation process, and the fermentation conditions are as follows: 0.8to2.0 percent of colloidal chitin is taken as a carbon source, the initial pH value of the fermentation fluid is 5.5 to 7.0, 0.8 to 1.5 percent peptone is taken as a nitrogen source, 0.1 percent Tween- 80 is taken as a surfactant, the fermentation time is 48 to 72h, the vaccination amount is 0.8 to 2 percent, and the rotational speed of a shaking bed is 100 to 180r / min. The invention has the following advantages that: 1. the paecilomyces lilacinus strain has fast growth, the producing capacity of chitin is strong; 2. the invention can decompose the body walls of flies and maggots and can growon the body walls of flies and maggots or a culture medium which is prepared by the decomposition products of the body walls and water; 3. the invention can be colonized at the root of a tobacco plantforresisting the infection of the tobacco black shank; 4. the invention can effectively overcome the antibacterial effect of the soil and maintain the suppression capacity of the tobacco black shankfora long time.

Description

Technical field [0001] The invention relates to the field of microorganisms, and more specifically, to a strain and fungus used for plant root diseases, especially for preventing and treating tobacco black shank. Background technique [0002] Tobacco black shank was first discovered and described by Breda de Haan in Java, Indonesia in 1896, and named it Phytophthroa parasiticavar.nicotianae (Breda de Haan) Tucker, a tobacco caused by this pathogen Black Shank disease is one of the major tobacco diseases in the world with widespread and serious damage. Tobacco black shank can infect and damage tobacco at any growth stage, but it mainly damages field tobacco plants. The onset of the seedling stage often causes the tobacco seedlings to die in pieces and become damping-off; the onset of the field stage can cause the entire tobacco plant to wither suddenly and accompanied by the blackening of the root or stem base, causing irreparable losses. After the 1980s in my country, with the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/02A01P3/00C12R1/79
Inventor 奚家勤尹启生宋纪真魏春阳周汉平薛超群蔡宪杰杨军王广山
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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