Bacterial strain for preventing and controlling tobacco black shank and bacterium agent thereof
A technology of tobacco black shank and bacterial agent, applied in the field of microorganisms, can solve the problems of tobacco production loss, etc., and achieve the effects of overcoming the antibacterial effect, strong chitinase production ability, and fast growth
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Embodiment 1
[0015] Example 1 Liquid formulation
[0016] The original species of Paecilomyces lilacinus (ZY-19-2) cultivated in a test tube is cultivated according to the three-stage process of triangular shake flask → seed tank → fermentation tank. In a 50L fermenter, filled with 30L of 2% colloidal chitin liquid medium, the initial pH of the fermentation broth was 6.5, 1.0% peptone as the nitrogen source, 0.1% Tween 80 as the surfactant, and the temperature was 0.05mpa. Sterilize for 30 minutes, after cooling to room temperature, insert 2.0L of strain ZY-19-2 liquid seeds, culture temperature 28℃, ventilation volume 1.5M 2 / h, the stirring speed is 120r / min, after 48 hours, the culture solution can be directly used as a liquid fungicide for field production.
[0017] Method of using Paecilomyces lilacinus (ZY-19-2) liquid fungicide: Dosage is 50L / mu, in Fenchen Township, Xiang County, apply the pesticide four times to tobacco plants in the field period that had black shank disease: First Th...
Embodiment 2
[0018] Example 2 Solid formulation
[0019] Diatomaceous earth and peat are used to prepare the carrier adsorbent for the fermentation broth. The preparation method of the carrier adsorbent is to use 1 kg of diatomaceous earth (60%) and peat (40%) as the carrier per liter of culture broth. The amount of spores will be 2×10 after natural drying. 8 A / g solid sterilization preparation, then packaged into a product for field production.
[0020] How to use the solid fungicide of Paecilomyces lilacinus (ZY-19-2): 1. Before the tobacco seedlings are transplanted, the solid fungicides are sprayed in the planting holes, and 15g of solid fungicides are applied to each hole, and then the tobacco seedlings are transplanted Planted in the hole for colonization. 2. Before the occurrence of black shank disease during transplanting of tobacco seedlings, a solid fungicide (15g) was sowed on the soil surface at the root of the tobacco stem, and 300 mL of clean water was poured.
[0021] The results...
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