Bacterial strain for preventing and controlling tobacco black shank and bacterium agent thereof
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A technology of tobacco black shank and bacterial agent, applied in the field of microorganisms, can solve the problems of tobacco production loss, etc., and achieve the effects of overcoming the antibacterial effect, strong chitinase production ability, and fast growth
Active Publication Date: 2008-07-16
ZHENGZHOU TOBACCO RES INST OF CNTC
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According to the survey of major smoking areas in 16 provinces and cities across the country from 1989 to 1991, except for Heilongjiang, which has not yet occurred, other smoking areas have occurred, and
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Embodiment 1
[0015] Embodiment 1 liquid preparation
[0016] The original species of Paecilomyces lilacinus (ZY-19-2) cultured in test tubes are cultivated according to the three-stage process of triangular shake flask→seed tank→fermentation tank. In a 50L fermenter, put 30L of 2% colloidal chitin liquid culture medium, the initial pH value of the fermentation broth is 6.5, with 1.0% peptone as nitrogen source, 0.1% Tween 80 as surfactant, through 0.05mpa Sterilize for 30 minutes, cool to room temperature, insert 2.0L strain ZY-19-2 liquid seeds, cultivate at 28°C, and ventilate at 1.5M 2 / h, the stirring speed is 120r / min, after 48 hours, the culture solution can be directly used as a liquid fungicide for field production.
[0017] Paecilomyces lilacinus (ZY-19-2) liquid fungicide application method: the dosage is 50L / mu, in Fenchen Township, Xiangxian County, apply the pesticide four times to the tobacco plants in the field stage where blackleg disease has occurred: the first The secon...
Embodiment 2
[0018] Embodiment 2 solid preparation
[0019] Diatomite and peat are used to prepare a carrier adsorbent for fermentation broth. The preparation method of carrier adsorbent is, use 1kg diatomite (60%) and peat (40%) as carrier adsorption by every liter of culture fluid, become spore amount after natural drying and be 2 * 10 8 pcs / g solid bactericidal preparation, and then packaged into a product for use in field production.
[0020] Paecilomyces lilacinus (ZY-19-2) solid fungicide usage method: 1. Before transplanting the tobacco seedlings, sprinkle the solid fungicide in the planting hole, apply 15g of solid fungicide in each hole, and then transplant the tobacco seedlings Planted in holes. 2. Before the occurrence of black shank on transplanting tobacco seedlings, spread the solid fungicide (15 g) on the soil surface at the roots of the tobacco stems, and pour 300 mL of clear water.
[0021] The results show that in the 3 acres of tobacco fields in Xiangcheng County, H...
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Abstract
The invention relates to a bacterial strain for preventing and treating tobacco black shank and the bacteria agent thereof, which is characterized in that: the invention adopts paecilomyces lilacinus ZY-19-2, the collection number is CCTCCNO: M207196, as the original bacterial strain to prepare the bacteria agent through a fermentation process, and the fermentation conditions are as follows: 0.8 to 2.0 percent of colloidal chitin is taken as a carbon source, the initial pH value of the fermentation fluid is 5.5 to 7.0, 0.8 to 1.5 percent peptone is taken as a nitrogen source, 0.1 percent Tween-80 is taken as a surfactant, the fermentation time is 48 to 72h, the vaccination amount is 0.8 to 2 percent, and the rotational speed of a shaking bed is 100 to 180r/min. The invention has the following advantages that: 1. the paecilomyces lilacinus strain has fast growth, the producing capacity of chitin is strong; 2. the invention can decompose the body walls of flies and maggots and can grow on the body walls of flies and maggots or a culture medium which is prepared by the decomposition products of the body walls and water; 3. the invention can be colonized at the root of a tobacco plant for resisting the infection of the tobacco black shank; 4. the invention can effectively overcome the antibacterial effect of the soil and maintain the suppression capacity of the tobacco black shank for a long time.
Description
technical field [0001] The invention relates to the field of microorganisms, more precisely, relates to a plant root disease, especially a bacterial strain and bacterial agent for preventing and treating tobacco black shank. Background technique [0002] Tobacco black shank bacteria was first discovered and described by Breda de Haan in Java, Indonesia in 1896, and named it Phytophthroa parasiticavar.nicotianae (Breda de Haan) Tucker), the tobacco caused by the bacteria Black shank is one of the world's major diseases of tobacco which is widely distributed and causes serious damage. Tobacco black shank can infect and damage tobacco at any growth stage, but it mainly damages field tobacco plants. The onset at the seedling stage often causes the tobacco seedlings to die in pieces and become damped-off; the onset at the field stage can cause the entire tobacco plant to wither suddenly and accompanied by the symptoms of blackening of the roots or stem bases, resulting in irrepa...
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