Bacterial strain for preventing and controlling tobacco black shank and bacterium agent thereof

Abstract

The invention relates to a bacterial strain for preventing and treating tobacco black shank and the bacteria agent thereof, which is characterized in that: the invention adopts paecilomyces lilacinus ZY-19-2, the collection number is CCTCCNO: M207196, as the original bacterial strain to prepare the bacteria agent through a fermentation process, and the fermentation conditions are as follows: 0.8 to 2.0 percent of colloidal chitin is taken as a carbon source, the initial pH value of the fermentation fluid is 5.5 to 7.0, 0.8 to 1.5 percent peptone is taken as a nitrogen source, 0.1 percent Tween-80 is taken as a surfactant, the fermentation time is 48 to 72h, the vaccination amount is 0.8 to 2 percent, and the rotational speed of a shaking bed is 100 to 180r / min. The invention has the following advantages that: 1. the paecilomyces lilacinus strain has fast growth, the producing capacity of chitin is strong; 2. the invention can decompose the body walls of flies and maggots and can grow on the body walls of flies and maggots or a culture medium which is prepared by the decomposition products of the body walls and water; 3. the invention can be colonized at the root of a tobacco plant for resisting the infection of the tobacco black shank; 4. the invention can effectively overcome the antibacterial effect of the soil and maintain the suppression capacity of the tobacco black shank for a long time.

Description

technical field [0001] The invention relates to the field of microorganisms, more precisely, relates to a plant root disease, especially a bacterial strain and bacterial agent for preventing and treating tobacco black shank. Background technique [0002] Tobacco black shank bacteria was first discovered and described by Breda de Haan in Java, Indonesia in 1896, and named it Phytophthroa parasiticavar.nicotianae (Breda de Haan) Tucker), the tobacco caused by the bacteria Black shank is one of the world's major diseases of tobacco which is widely distributed and causes serious damage. Tobacco black shank can infect and damage tobacco at any growth stage, but it mainly damages field tobacco plants. The onset at the seedling stage often causes the tobacco seedlings to die in pieces and become damped-off; the onset at the field stage can cause the entire tobacco plant to wither suddenly and accompanied by the symptoms of blackening of the roots or stem bases, resulting in irrepa...

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Problems solved by technology

According to the survey of major smoking areas in 16 provinces and cities across the country from 1989 to 1991, except for Heilongjiang, which has not yet occurred, other smoking areas have occurred, and Henan, Shandong, Anhui, Yunnan and other provinces have occurred more frequently, with a general incidence rate of 5%- 15%, and more than 30% in severe cases, causing great losses to tobacco production

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