Cultivation of sheath blight-resisting transgenic paddy rice and special vector

A technology of encoding genes and recombinant vectors, applied in genetic engineering, plant gene improvement, application, etc., can solve problems such as incorrect methods, untimely medication, and poor control effects

Active Publication Date: 2013-03-13
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the current situation of high fertility, large populations, and a large number of young and middle-aged laborers in rural areas, often due to untimely or incorrect methods of medication, the control effect is gene

Method used

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  • Cultivation of sheath blight-resisting transgenic paddy rice and special vector
  • Cultivation of sheath blight-resisting transgenic paddy rice and special vector
  • Cultivation of sheath blight-resisting transgenic paddy rice and special vector

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, containing GAFP 2 High-efficiency expression vector pCambia2300-35S-GAFP 2 build

[0036] Synthetic GAFP 2 Gene, the nucleotide sequence of GAFP2 is sequence 1 in the sequence listing, and the amino acid sequence encoded by the gene is sequence 2 in the sequence listing. Sequence 1 in the above sequence list consists of 516 nucleotides, the 1st-84th position from the 5' end is the gene encoding the signal peptide, and the 85th-516th position from the 5' end is the gene encoding GAFP 2 protein gene. Sequence 2 in the above sequence list consists of 171 amino acids, the 1-28th position from the amino terminal is a signal peptide, and the 29-171st position from the amino terminal is GAFP 2 protein.

[0037] GAFP 2 The gene (sequence 1 in the sequence listing) was connected to pUCm-T (purchased from Shanghai Sangon Bioengineering Co., Ltd.), and the vector pUCm-GAFP was constructed 2 .

[0038] Digest pUCm-GAFP with XbaI and SacI 2 , to obtain GAFP af...

Embodiment 2

[0040] Embodiment 2, transfer GAFP 2 Study on the Acquisition and Function of Rice

[0041] 1. Transfer to GAFP 2 harvesting of rice

[0042] 1. Convert to GAFP 2 harvesting of rice

[0043] 1) the expression vector pCambia2300-35S-GAFP obtained in Example 1 2 Transformed into Agrobacterium tumefaciens LBA4404 (purchased from Invitrogen) to obtain Agrobacterium tumefaciens LBA4404 / pCambia2300-35S-GAFP 2 .

[0044] 2) Pick Agrobacterium tumefaciens LBA4404 / pCambia2300-35S-GAFP respectively 2 Single colonies were inoculated in 20ml of YEP medium containing 50mg / L kanamycin, 50mg / L rifampicin and 30mg / L streptomycin, and cultured overnight at 28°C and 280rpm until the OD600 was 0.7-0.8. Then centrifuge at 5000 rpm, discard the supernatant, and resuspend the pellet with NB-liquid medium. Finally, the rice "Xudao 3" (Xu Jun, Duan Xiangmao, Liu Qiang, Yang Bo, Xu Zongjin, Zhou Wei, Chen Liugen and Zhong Weigong. 2008. Experimental Research on Super-high Yield Cultivation of ...

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Abstract

The invention discloses an application of GAFP2 protein in cultivating sheath blight-resisting transgenic paddy rice and a special vector. The invention provides an application of GAFP2 protein or an encoding gene thereof or a recombinant vector in regulating and controlling plant disease resistance. The amino acid sequence of a GAFP2 protein is a sequence 2 inside a sequence table or the (29-171)th site amino acid residue of the sequence 2 inside the sequence table and from an N'tail end. Experiments improve that the GAFP2 is inducted into paddy rice so that transgenic paddy rice is obtained, therefore the resistance of paddy rice sheath blight can be effectively enhanced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kind of breeding and special carrier of transgenic rice resistant to sheath blight. Background technique [0002] Rice sheath blight is a soil-borne fungal disease caused by Rhizoctonia solani Kühn. Since the 1960s, along with the popularization of dwarf varieties, dense planting and large-scale application of chemical fertilizers, sheath blight has become one of the three major diseases of rice worldwide, generally causing a 15%-20% reduction in yield, and can reduce yield in severe cases 60%-70%. [0003] The disease is a leaf sheath disease that begins at the base of the plant. Under the conditions of rice populations, two disease peaks are generally formed from the late tillering stage to the early jointing stage and about 20 days before and after the heading stage, especially the latter peak. It spreads horizontally and makes the surrounding healthy plants sick, and the dam...

Claims

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Application Information

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IPC IPC(8): C12N15/84C12N1/21C12N5/10A01H5/00
Inventor 储成才王义琴刘丰泽陈帅
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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