792 results about "Plant disease resistance" patented technology

The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include nucleic acid molecules encoding Fus6 polypeptides derived from Agrotis ipsilon that possess antimicrobial properties, particularly fungicidal properties. Further provided are plant cells plants, and seed thereof, transformed with the nucleic acid molecules of the invention so as to confer disease resistance on the plant.

The present invention discloses one kind of pesticide composition containing oligosaccharide in effective amount and carrier. The present invention also discloses its preparation process and use in resisting plant diseases. The composition of the present invention can stimulate the defense reaction of rice and other plants to strengthen disease resistance.

Methods for producing pathogen-inducible promoters for the expression of genes in plants are provided. The pathogen-inducible promoters are inducible by one, two, three, or more plant pathogens. Methods for producing R genes that are inducible in a plant by more than one plant pathogen are further provided. Additionally, provided are R genes and other nucleic acid molecules comprising the pathogen-inducible promoters and that are made by such methods as well as plants, plant parts, plant cells, seeds, and non-human host cells comprising the R genes and other nucleic acid molecules

The invention provides a brown planthopper resistant rice gene Bph9 as well as a molecular marker and application thereof. The brown planthopper resistant rice gene Bph9 has a nucleotide sequence as shown in SEQ ID No.1 and a cDNA (complementary Desoxvribose Nucleic Acid) sequence as shown in SEQ ID No.2. The Bph9 gene is located between molecular markers InD2 and RM28466. The molecular markers closely linked with the gene also includes one of RM28438, InD28450, InD28453, InD14, InD28432, RM28481 and RM28486 which can be used for sieving the rice containing the brown planthopper resistant gene Bph9. The Bph9 gene belongs to the NBS-LRR (Nucleotide Binding Site-Leucine Rich Repeat) gene family; the encoded protein is related to the plant disease resistance; the Bph9 gene is transferred into common rice by virtue of genetic transformation and hybridization, and thus the resistance of rice to brown planthopper can be improved, so that the damage of brown planthopper is decreased, and the purposes of yield increase and yield stabilization are realized.

This invention provides a novel method to confer disease resistance to plants. Plant plastids are transformed using a plastid vector which contains heterologous DNA sequences coding for a cytotoxic antimicrobial peptide. Transgenic plants are capable of fighting off phytopathogenic bacterial infection.

The invention discloses a special basal fertilizer used in ecological tobacco leaf plantation. The invention belongs to the technical field of organic fertilizers. The special basal fertilizer used in ecological tobacco leaf plantation comprises components of, by weight: 19 to 23% of fermented cake fertilizer, 43 to 47% of biogas residue, 17 to 21% of common calcium superphosphate, 2.3 to 2.7% ofpotassium nitrate, 0.27 to 0.32% of borax, 0.8 to 0.9% of zinc sulfate heptahydrate, 9.5 to 9.9% of potassium sulfate, 0.05 to 0.15% of potassium humate, and 0.64 to 0.68% of mixed microbial manure. The basal fertilizer is prepared through steps that: (1) cake fertilizer and biogas residue are dried and crushed, such that a basic mixture is obtained; (2) the mixed microbial manure is added; and (3) common calcium superphosphate, potassium nitrate, borax, zinc sulfate heptahydrate, potassium sulfate, and potassium humate are added. With the basal fertilizer provided by the invention, soil micro ecological environment can be repaired or protected, tobacco plant disease resistance can be improved, quality of fire-cured tobacco can be improved, and ecological tobacco production can be continuously and healthily developed.

The invention provides a brown planthopper resistance gene Bph14 in rice. The gene has a nucleotide sequence shown in SEQ ID No. 1 and a cDNA sequence thereof is shown in SEQ ID No.2. The gene Bph14 of the invention belongs to CC-NBS-LRR gene family, and coded protein is related to plant disease resistance. The gene Bph14 has function of resisting the brown planthopper. Through genetic transformation and hybridization, the gene Bph14 is transferred into normal rice varieties, which can improve the resistance of the rice to the brown planthopper, thus reducing the damage caused by the brown planthopper and achieving the purposes of increasing and stabilizing the production of rice.

The invention relates to the technical field of plant genetic engineering, in particular relates to isolating clone and functional verification of a DAN fragment containing a gene OsWRKY45-2 related to rice disease resistance. The gene OsWRKY45-2 codes WRKY proteinoids and can endow rice with resistance to the diseases caused by bacterial pathogenic bacteria, namely the leaf blight bacteria (Xanthomonas oryzae pv.oryzae) and fungal pathogenic bacteria, namely the rice blast bacteria (Magnaporthe grisea). The fragment and the exogenous regulatory sequence thereof are directly transferred into rice, thus the resistance capability of transgenic rice with over-expression OsWRKY45-2 against bacterial blight in and rice blast is improved remarkably.

The invention discloses a high-nutrition foliage fertilizer for improving plant stress resistance. A filtrate, namely a finished product is obtained by the following steps: mixing and dissolving water, compound amino acid, monopotassium phosphate and citric acid; adding alcohol and salicylic acid into the mixture for a coupling reaction; orderly adding Na-EDTA, FeSO4, ZnSO4, MnSO4, CuSO4, MgSO4, H3BO3, vitamin C, Na2MoO4, KNO3, and urea respectively for a hybrid reaction; and filtering after the complete reaction. The high-nutrition foliage fertilizer has simple preparation technology and low cost, can effectively improve the stress resistance of plants, directly promote the root growth of the plants, adjust the physiological function of the plants, improve the resistances of the plants such as disease resistance, drought resistance, cold resistance and the like, has the characteristics of full nutrition, good absorption effect and the like, and provides due guarantee for the normal growth of the plants, thereby laying a solid material basis for improving the quality of farm products.

The invention relates to the technical field of plant disease resistance, and discloses a method for identifying sugarcane smut resistance. The method for identifying sugarcane smut resistance comprises the steps of: firstly carrying out separate culture to obtain sugarcane smut alien mating type (+or -) haplospores by using a trace amount of fresh sugarcane smut teliospore; and then carrying out the steps of culture enlargement of strains, inoculation, investigation of disease incidence, resistance evaluation and the like. The method for identifying sugarcane smut resistance has the outstanding substantial characteristics and is improved remarkably as follows: 1. the inoculation need can be met by separate culture of only an extremely trace amount of sugarcane smut teliospore, and the working amount is low; 2. the alien mating type strains can be stored at an ultra-low temperature and can be used as the inoculation source after subjected to activation and enlarge culture without repeated taking of strains and separate culture for each time; 3. in the method for identifying sugarcane smut resistance, the resistance identifying period is short, and the sensitivity is high; and 4. the method for identifying sugarcane smut resistance can also be used for identifying the type of smut biological strains resisted by the tested varieties, and effectively guiding smut-resisting seed breeding of sugarcane and reasonable collocation of varieties in a sugarcane area.

The present invention provides a selenium-rich rice compound fertilizer. The compound fertilizer comprises the following raw materials in parts by weight: 400-600 parts of a nitrogen-phosphorus-potassium fertilizer, 50-70 parts of nitro humic acid, 2-8 parts of organic trace elements, 3-7 parts of inorganic trace elements, 10-20 parts of biomass slag, and 1-2 parts of inorganic selenium salt. The organic trace elements comprise a sucrose sugar complex and a humic acid complex. The selenium-rich rice compound fertilizer provided by the present invention has the advantages that the selenium-rich rice compound fertilizer is high in rice yield, high in plant disease resistance and suitable to be applied in large area; rice ears are relatively large and heavy; and because selenium-rich rice contains selenium that is essential for human bodies and animals, the selenium-rich rice is high in nutritional value and beneficial for health.

The invention discloses a green agricultural compound Chinese herbal medicine bactericide which is prepared from the following Chinese herbal medicines: schisandra chinensis, eucommia ulmoides, alpinia oxyphylla, bighead atractylodes rhizome, bletilla striata, radix scutellariae, radix stemonae, semen strychni, sophora flavescens, arundo donax, radix ophiopogonis, dandelion, liquorice, rheum officinale, radix lithospermi, clove, cortex magnoliae officinalis, fructus forsythiae and coptis chinensis; the bactericide is prepared by extracting the Chinese herbal medicines by alcohol; the bactericide is mainly used for controlling various diseases of various crops caused by fungi, so that the disease resistance and the immunologic function of the crops can be improved. After the green agricultural compound Chinese herbal medicine bactericide is used, the defects that the environment is polluted by the chemical pesticide and the drug resistance is easily caused can be overcome, and the problems that the biological pesticide is small in sterilization range and slow in pesticide effect can be solved; the green agricultural compound Chinese herbal medicine bactericide can be used for controlling various fungal and bacterial diseases of plants, does not cause drug resistance after being used for a long time, and is less in environmental pollution and harmless to people and livestock.

The invention discloses an application of Arabidopsis glycosyltransferase gene UGT73C7 in improving plant disease resistance, wherein the nucleotide sequence of the glycosyltransferase gene UGT73C7 is shown in SEQ ID No. 1 and is cloned by RT-PCR from Arabidopsis thaliana. The ene UGT73C7 is used to construct a plant overexpressing vector for plant transgene operation, so as to obtain the transgenic plants. The detection results show that the disease resistance of the transgenic plants is significantly improved, suggesting that new disease-resistant plants can be created after the implementation of the application disclosed by the invention, and the application can be used for the subsequent crop variety improvement and has great significance for China's agricultural production.

The invention relates to the technical field of the plant genetic engineering, in particular to the isolation and cloning and functional verification of the DNA fragment of oryza officinalis anti-Xanthomonas oryzae major gene Xa3/Xa26-2. The Xa3/Xa26-2 gene is used to code the leucine-rich protein kinase protein. The Xa3/Xa26-2 gene ensures that rice can resist diseases caused by bacterial pathogen-Xanthomonas oryzae pv. oryzae. The DNA fragment and the regulatory sequence thereof are directly transferred in rice, and the resistance capability to Xanthomonas oryzae of the transgenic rice carrying Xa3/Xa26-2 is significantly enhanced.

The invention relates to glucose-rich spirulina polysaccharide and an application thereof in a plant green pesticide and an anti-tumor drug. High-sugar spirulina obtained through nutritional regulation is used as a raw material and is subjected to hot water extraction, protein removal with a flocculant, concentration, precipitation and freeze drying to obtain the water-soluble high-sugar spirulina polysaccharide having the molecular weight of 10-200 kDa; the spirulina polysaccharide comprises the monosaccharides mainly comprising rhamnose, fucose, arabinose, xylose, mannose, galactose, glucose, glucuronic acid and galacturonic acid, and is characterized in that the glucose content is more than 50% in the monosaccharide composition. The spirulina polysaccharide can obviously induce plant disease resistance, has obvious anti-tumor effect on tumor, and can be used as the biological pesticide and the anti-tumor drug.

The invention relates to a small molecular compound for enhancing plant stress resistance, and firstly reveals that small molecular compound AM1 has similar physiological activity with ABA, can significantly enhance plant tolerance to abiotic stress (commonly including cold, drought, salinity, osmotic pressure, heat and the like) widely existing in environment, and has higher application value.

The invention discloses pig feed capable of improving disease resistance of pigs. The pig feed capable of improving the disease resistance of pigs is characterized by comprising, by weight, 50-60 parts of bran, 5-10 parts of rice bran, 12-18 parts of bean cakes, 3.2-3.8 parts of fish meal, 0.8-1.2 parts of bone meal, 3-8 parts of cushaw stems, 0.10-0.20 part of lysine, 0.10-0.20 part of glutamic acid, 0.3-0.5 part of salt, 1-3 parts of garlic, 1-3 parts of wrinkled giant hyssop, 3-5 parts of red dates, 0.5-1.0 part of polygala tenuifolia, 0.5-0.8 part of endothelium corneum gigeriae galli, 3-5 parts of dandelion, 0.05-0.15 part of multivitamin, 0.4-0.6 part of gunk and 3-5 parts of distillers' grains. The pig feed contains the agastache rugosus which can invigorate stomach and promote appetite of the pigs. The red dates and the polygala tenuifolia can soothe the nerves, enable the pigs to be settled, reduce activity of the pigs and physical output, and fatten as soon as possible. The garlic and the dandelion can invigorate stomach, sterilize, detoxify, improve immunity of the pigs, and reduce diseases and enotoxin of the pigs, and therefore the pig feed can improve meat quality ofthe pigs, and is environmental-friendly and safe.

The invention discloses a method of researching the change of a proteome of rice responding rice blast bacterial infection through an iTRAQ technology and belongs to the field of plant biotechnology. The method includes following steps: A. extracting a plant materials and leaf protein; B. performing further FASP enzymolysis, peptide fragment marking, SCX classification and LC-MS analysis to the protein sample obtained in the step A; C. performing data query and quantitative analysis to the result after the LC-MS analysis to obtain the change situation of the whole proteome during an interaction process between rice and rice blast bacteria. The method can quickly and effectively screening a candidate gene relative to disease resistance of rice, can be used for researching the candidate gene relative to the disease resistance of the rice well with the proteomics researching method, iTRAQ, which is high-throughput and accurate, can be widely used for researching and developing the characters of plant disease resistance and breeding new plant varieties being strong in disease resistance with combination of the transgenic technology.

The invention discloses oligomeric acid iodine as well as a preparation method and application thereof. The molecular structural formula of the provided oligomeric acid iodine is shown in the formula I, wherein m>=2, n>=2, and R1, R2, R3 and R4 are oligomeric glucose (acid) groups, oligomeric N-acetyl glucose (acid) groups, oligomeric D-amino glucose (acid) groups, oligomeric galactose (acid) groups, oligomeric gulose (acid) groups, oligomeric mannose (acid) groups or oligomeric xylose (acid) groups. The preparation method of the oligomeric acid iodine comprises the following steps of: mixing oligomeric acid or oligomeric acid salt with iodine in a mixed solvent of water and alcohol, stirring, controlling the temperature at 50-70 DEG C, and reacting to obtain the oligomeric acid iodine. The oligomeric acid iodine ensures the stability of iodine in the structural matter and has extremely strong effect of contact skilling plant pathogenic bacteria, keeps and strengthens the plant disease resistance induced by oligose, and has rapid and persistent effect for controlling plant diseases. The formula I is disclosed in the specification.

The invention discloses application of disease resistance-related protein to regulation and control of plant disease resistance. In the application disclosed by the invention, the disease resistance-related protein is A1), A2) or A3), wherein A1) is protein with an amino acid sequence shown in the sequence 2; A2) is protein which is obtained by performing the replacement and/or missing and/or addition of one or more of amino acid residues on the amino acid sequence in the sequence 2 and is related to the plant disease resistance; A3) is fusion protein obtained by connecting a tag at the N end or/and the C end of the A1) or A2). An experiment proves that the disease resistance-related protein disclosed by the invention and a coding gene thereof can regulate and control the plant disease resistance; the plant disease resistance can be improved through improving the expression of the disease resistance-related protein, and the plant disease resistance can be reduced through reducing the expression of the disease resistance-related protein, therefore, the plant disease resistance can be regulated and controlled by utilizing the disease resistance-related protein and the coding gene thereof.

The invention disclosed a compound fertilizer which comprises basic nutrition fertilizer including nitrogen, phosphorus and potassium, and also comprises gamma-polyglutamic acid and seaweed extract, wherein the gamma-polyglutamic acid accounts for 0.1-2% of the basic nutrition fertilizer, and the seaweed extract accounts for 0.5-2% of the basic nutrition fertilizer. The compound fertilizer containing the gamma-polyglutamic acid and the seaweed extract has high activity biological function, and is capable of improving the yield and the resistance capability such as disease resistance, drought resistance, cold resistance, lodging resistance and the like of crops. Therefore, the compound fertilizer can be widely applied to grain crops such as wheat, rice, corn and the like, and cash crops such as tomatoes, melons, grapes and the like. One-off fertilization can be realized without topdressing, and the yield of the crops can be increased by 10-30%.

The invention relates to the technical field of plant gene engineering, in particular to the isolation cloning and function verification of a DNA fragment containing a rice disease resistance related gene OsWRKY45-1. A nucleotide sequence of the OsWRKY45-1 gene and an amino acid sequence encoded by the gene are shown in SEQ ID NO: 1. Genetically transformed rice of which the expression level of the OsWRKY45-1 gene is remarkably reduced apparently strengthens the resistance on xanthomonas oryzae pv. oryzae and xanthomonas oryzae pv.oryzicola, which proves that the OsWRKY45-1 gene is a negative regulatory factor in reactions of resisting bacterial blight and bacterial stripe of rice, and the disease resistance of the rice can be improved by inhibiting the expression of the OsWRKY45-1 gene.