Method for identifying sugarcane smut resistance

A technology for identification of resistance and smut, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as small workload, long identification period, and large amount of teliospores required, and achieve low workload , short identification period and high sensitivity

Inactive Publication Date: 2011-10-05
GUANGZHOU SUGARCANE IND RES INST +1
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Problems solved by technology

[0007] The purpose of the present invention is to propose a sugarcane smut resistance identification for existing identification of sugarcane smut resistance. method, this method has the characteristics of short duration, small workload, high ef

Method used

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Embodiment Construction

[0037] A kind of sugarcane smut resistance identification method, carries out according to the following steps:

[0038] Step A. Isolation and cultivation of outcrossing type (+, -) haploid basidiospores of S. cane smut

[0039] A1. In May 2010, freshly extracted sugarcane smut single whip was collected. On the ultra-clean workbench, pick a small amount (about 8ug) of fresh teliospores of sugarcane smut and add them to YEPS liquid medium (1mL) After fully shaking into a suspended turbid solution, draw 100uL of the turbid solution and fully dilute it with YEPs liquid medium (2 mL) to obtain a uniform dilution. There are 50-100 teliospores of S. smut in each ml of dilution.

[0040] A2. Draw 100uL of the diluted solution and spread it evenly on the YEPS solid medium, and culture it at 28-30°C for 2-3 days; a white "wool-like" single colony is obtained.

[0041] A3. Pick one of the white "wool-like" single colonies, fully dilute it with YEPS liquid medium to a dilution of 50-1...

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Abstract

The invention relates to the technical field of plant disease resistance, and discloses a method for identifying sugarcane smut resistance. The method for identifying sugarcane smut resistance comprises the steps of: firstly carrying out separate culture to obtain sugarcane smut alien mating type (+or -) haplospores by using a trace amount of fresh sugarcane smut teliospore; and then carrying out the steps of culture enlargement of strains, inoculation, investigation of disease incidence, resistance evaluation and the like. The method for identifying sugarcane smut resistance has the outstanding substantial characteristics and is improved remarkably as follows: 1. the inoculation need can be met by separate culture of only an extremely trace amount of sugarcane smut teliospore, and the working amount is low; 2. the alien mating type strains can be stored at an ultra-low temperature and can be used as the inoculation source after subjected to activation and enlarge culture without repeated taking of strains and separate culture for each time; 3. in the method for identifying sugarcane smut resistance, the resistance identifying period is short, and the sensitivity is high; and 4. the method for identifying sugarcane smut resistance can also be used for identifying the type of smut biological strains resisted by the tested varieties, and effectively guiding smut-resisting seed breeding of sugarcane and reasonable collocation of varieties in a sugarcane area.

Description

technical field [0001] The invention relates to the technical field of plant disease resistance identification, in particular to a sugarcane smut resistance identification method, and also relates to a sugarcane smut physiological race identification method. Background technique [0002] by Saccharomyces sativa ( U. scitaminea ) caused by sugarcane smut is an important sugarcane disease worldwide, and it is also the most economically harmful sugarcane disease in my country. The most economical and effective way to prevent and control the disease is disease-resistant breeding. The basis and key of disease-resistant breeding is the identification method of disease resistance, that is, the high resistance varieties of sugarcane smut are selected for breeding through the identification method of disease resistance. The identification method of sugarcane smut resistance commonly used in my country is to use artificially collected sugarcane smut mixed with teliospores to impregna...

Claims

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Application Information

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IPC IPC(8): A01G7/06A01G1/00
Inventor 沈万宽姜子德陈健文习平根邓海华刘睿
Owner GUANGZHOU SUGARCANE IND RES INST
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