49 results about "Sugarcane smut" patented technology

The invention relates to the technical field of plant disease resistance, and discloses a method for identifying sugarcane smut resistance. The method for identifying sugarcane smut resistance comprises the steps of: firstly carrying out separate culture to obtain sugarcane smut alien mating type (+or -) haplospores by using a trace amount of fresh sugarcane smut teliospore; and then carrying out the steps of culture enlargement of strains, inoculation, investigation of disease incidence, resistance evaluation and the like. The method for identifying sugarcane smut resistance has the outstanding substantial characteristics and is improved remarkably as follows: 1. the inoculation need can be met by separate culture of only an extremely trace amount of sugarcane smut teliospore, and the working amount is low; 2. the alien mating type strains can be stored at an ultra-low temperature and can be used as the inoculation source after subjected to activation and enlarge culture without repeated taking of strains and separate culture for each time; 3. in the method for identifying sugarcane smut resistance, the resistance identifying period is short, and the sensitivity is high; and 4. the method for identifying sugarcane smut resistance can also be used for identifying the type of smut biological strains resisted by the tested varieties, and effectively guiding smut-resisting seed breeding of sugarcane and reasonable collocation of varieties in a sugarcane area.

The invention provides a strain of Paenibacillus jamilae, and a fermentation product, a preparation method and application thereof. The name of the strain of Paenibacillus jamilae is Paenibacillus jamilae WAC199 which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) located in Guangzhou of China on October 31, 2018, the preservation number is GDMCC No: 60470, and the strain ofPaenibacillus jamilae has a remarkable inhibition effect on citrus canker pathogens and sporisorium scitamineum. The invention also provides a fermentation culture method of a metabolic active substance of the strain, and the preparation method is simple and easy to operate; the metabolic active substance also has a remarkable antagonistic effect, and keeps high and stable bacteriostatic activityunder high temperature, a wide pH range and ultraviolet irradiation. The invention provides a more economic, effective and environment-friendly solution for biological prevention and control of citrus canker and sugarcane smut, and a good application prospect in prevention and control of pathogenic bacteria is provided.

The invention belongs to the technical field of biological prevention and control, and particularly discloses a pseudomonas guariconensis strain ST4 and an application thereof in the prevention and the treatment of sugarcane smut. The strain is preserved in CCTCC (China Center for Type Culture Collection) on September 14, 2015, and the strain preservation number is CCTCC NO. M 2015526. The invention discovers that the strain ST4 has a good inhibition or delay effect on the sexual matching of the sugarcane smut; the formation of dicaryon mycelia is blocked, so that the sporisorium scitaminea cannot normally infect the sugarcane; a novel development resource is provided for replacement of chemical synthetic bactericides by microbes; and the strain ST4 can be used as biopesticide to be developed and utilized.

The invention relates to Bacillus subtilis HAS and an application thereof in preventing and treating sugarcane smut. The bacterial strain is named as Bacillus subtilis HAS, and is preserved in the general microorganism center of the Chinese microorganism bacterial strain preservation and administration committee, and the preservation number is CGMC No.6590. The use of the Bacillus subtilis HAS provided by the invention is the application of preventing and treating sugarcane smut; and a pot experiment shows that the Bacillus subtilis HAS has very good suppression function to sugarcane smut, the prevention effect can be 100% due to soaking seed, and the experiment shows that the bacterial strain is a biocontrol strain having potential meaning in preventing and treating sugarcane smut germs.

The invention discloses a fertilizer special for sugarcane and a production method thereof. Raw materials of the fertilizer include inorganic fertilizers, organic materials, secondary element, microelement and botanical fungicides. The fertilizer is prepared from, by weight, 30% to 38% of total nutrients (N, P2O5 and K2O), larger than or equal to 15% of the organic materials, 0.1% to 0.5% of the secondary element, 0.04% to 0.1% of the microelement and 1% to 10% of the botanical fungicides (please see GB18877-2009 Organic-Inorganic Compound Fertilizer National Standard for the notation). According to the fertilizer special for the sugarcane, the ratio of nitrogen to phosphorus to potassium is reasonable, fertilizer shortage is avoided in the growth stages of the sugarcane, and the secondary element, the microelement and the organic materials are provided; in addition, cane beetles and borers can be effectively prevented, and sugarcane smut can be effectively inhibited; as for the sugarcane planted with the fertilizer special for the sugarcane, emergence seedlings are tidy, stalks are thick, lodging is not prone to occurrence, the number of plant diseases and insect pests is small, the average increase yield per mu ranges from 1,000 kg to 2,000 kg, the sugar content is increased by 1% to 3%, and economic benefits are greatly increased.

The invention discloses a strain, and the strain is a Bacillus amyloliquefaciens ZHR0 strain collected in the China Center for Type Culture Collection (CCTCC) with a collection number of CCTCC NO:M 2014577. The invention further discloses a preparation method of ZHR0 biocontrol microbial inoculum. Experiments show that the control efficiency of the ZHR0 biocontrol microbial inoculum prepared by the preparation method to the sugarcane smut is up to 53.98%, which indicates that the Bacillus amyloliquefaciens ZHR0 strain has good prevention and control effects on the sugarcane smut.

The invention belongs to the field of biological pesticides, and discloses a sugarcane endophytic bacillus amyloliquefaciens RGB15 strain and an application thereof. The bacillus amyloliquefaciens CGB15 strain is preserved in the Guangdong Province Microbial Culture Collection Center on October 17, 2019, and the preservation number is GDMCC No: 60742, and the preservation address is the 5th floorof No.59 building of No.100 courtyard of the Xianlie Middle Road of Guangzhou city. The bacillus amyloliquefaciens RGB15 strain has a strong inhibition effect on ustilago scitaminea, pyricularia grisea, peronophythora litchii, colletotrichum litchii and banana fusarium oxysporum, and has an excellent biological control effect. A microbial preparation prepared from the strain is simple in preparation and use method, has broad-spectrum disease resistance, has little influence on the ecological environment, does not influence the crop quality, and has an excellent application prospect.

The invention belongs to the field of biopesticides and discloses a pseudomonas fluorescens and a biological preparation and an application in preventing and controlling sugarcane smut. The pseudomonas fluorescens is named as pseudomonas fluorescens HN58 which is preserved in China Center for Type Culture Collection on October 24, 2014, wherein the address is Wuhan University, Wuhan and the preservation number is CCTCC No: M2014511. The strain is separated from rhizosphere soil of sugarcane and can be suitable for local natural environment. The strain has a relatively good inhibiting effect on sugarcane smut and is environment-friendly and non-toxic in source and less in influence on ecological environment; the culture condition is low in requirement, and the pseudomonas fluorescens has very good development and application prospects.

A smut inoculation method for sugarcane seedlings comprises the steps as follows: after seven true leaves of each seedling are grown out, the seedling is pulled out from an incubator, the middle-upper parts of the leaves are cut off, 2-3 old leaves at the base part are removed, a spore suspension with the ustilago scitaminea syd concentration of 1*10<7> spores / ml is adopted for soaking the middle-lower part of the pulled seedling for 10 min, and the seedling is sealed, placed in a room at the temperature of 25-28 DEG C overnight in a moisture preserving manner, transplanted in a nutrient bag next day and planted in the field after survived; and combination morbidity is researched during final selection, combination with the combination morbidity higher than 25% is eliminated, and seedlings with good and healthy characters are screened out to enter a next stage for observation. According to the invention, morbidity of the seedlings inoculated with ustilago scitaminea syd are remarkable, the reproducibility is good, the test cycle is short, the operability is high, the cost is low, and a large quantity of seedlings can be inoculated; and the method can be applied to a sugarcane variety breeding institution for new sugarcane variety breeding resistant to sugarcane smut.

The invention relates to the field of sugarcane pathogenic bacteria detection, and provides a sugarcane smut bacteria nest type polymerase chain reaction (PCR) quick detection method. The method comprises the steps of DNA extraction, primary PCR amplification, secondary PCR amplification and PCR amplification product detection, wherein universal primers ITS4 and ITS5 of a fungal ribosome genetic transcription spacer ITS are adopted in the primary PCR amplification; and specific primers Smut-L1 and Smut-R2 of a sugarcane smut ribosome genetic transcription spacer ITS are adopted in the secondary PCR amplification. The method has the substantial characteristics that: 1, the detection sensitivity is extremely high, and the smut bacteria can be accurately detected even if the sugarcane has nosmut symptom or is infected little by the smut bacteria; 2, the method is strong in specificity, low in interference of other bacteria and high in accuracy; and 3, the method has short time consumption and high detection speed, and is suitable for sugarcane variety introduction quarantine, toxin-free seedling quality detection, sugarcane smut resistance identification, early symptom-free leaf detection and the like.

The invention discloses an application of farnesol in preventing and treating the smut disease of sugarcane and corn. According to the application of farnesol in preventing and treating smut disease,the life history of sugarcane smut can be affected. Farnesol has better inhibiting effect on the germination of teliospore, the proliferation and growth of basidiospore and the sexual cooperation. Theformation of the binuclear mycelium can be effectively blocked, the sugarcane can not be normally infected with sugarcane smut, and the occurrence of the smut disease of the sugarcane can be effectively inhibited; the proliferation and growth of corn smut can be affected and the occurrence of corn smut can be inhibited.

The invention discloses application of a mycophenolic acid compound or a derivative or pharmaceutically acceptable salt thereof in preventing and treating culmicolous smut of sugarcane. The compound has a relatively good inhibiting effect on growth and sexual matching of sugarcane smut, and can block formation of dikaryotic mycelium, and the sugarcane smut cannot normally infect sugarcane, so that the culmicolous smut of sugarcane is effectively inhibited.

The invention relates to a method for identifying sugarcane smut resistance by utilizing an SCAR (Sequence Characterized Amplified Region) label. The method comprises the following steps of: extracting genome DNA; establishing a detection system of an SCAR-PCR (Polymerase Chain Reaction); and detecting an SCAR-PCR product. The method for identifying sugarcane smut resistance by utilizing the SCAR label has the characteristics of high sensitivity and lower dosage of DNA, and compared with conventional artificial inoculation field resistance identification, the method has the advantages of simple and convenient operation, short detection time, high accuracy, easy judgment, good repeatability, no environment condition influence and the like. The method provides a simple, convenient and effective smut resistance identification system for the evaluation of sugarcane germplasm resources and the approval of new varieties and has significance to scientific researches and production.

The invention discloses a method of inoculating Ustilago scitaminea to tissue culture seedlings of sugarcane. The method includes: with an injector, injecting a mixed spore suspension of '+' and '-' mated wild strains of Ustilago scitaminea and a mixed spore suspension of '+' and '-' mated mutant strains of Ustilago scitaminea respectively to inner parts, near growth spots, of the tissue culture seedlings of sugarcane, wherein the mixed spore suspension of the '+' and '-' mated strains is 1*107 per mL, 1 mL of the spore suspension is injected per seedling, and the tissue culture seedlings of sugarcane to be tested are tissue culture seedlings 35-45 cm in height. After Ustilago scitaminea is inoculated, origination is evident, operability is high, and pathogenicity test cycle is shortened;therefore, efficiency of screening Ustilago scitaminea pathogenic defective mutants is improved; technical support is provided for the identification and mechanism research on pathogenicity associatedgenes of Ustilago scitaminea; the method has important significance.

The invention provides an anti-drought efficient compound fertilizer specially used for sugarcane planting, which is characterized by comprising the following raw materials: plant straw, humic acid, urea, diammonium hydrogen phosphate, potassium sulfate, medical stone, animal waste, magnesium sulfate, zinc sulfate, fly ash, super absorbent resin, Chinese herbal medicine powder and bacillus licheniformis. The efficient compound fertilizer is capable of improving the sustainable anti-drought capacity of the soil, increasing the unit water utilization benefit by more than one time, obviously improving the sugarcane growth environment, providing necessary nutritional ingredients and trace elements for sugarcane growth and increasing the sugarcane yield, and has a good control effect on sugarcane smut.

The invention discloses a novel method for rapidly identifying the resistance level of sugarcane smut, which comprises the following steps: selecting a mature plant which grows vigorously, cutting off sugarcane tips, and binding the tail ends of stems with a plastic film; the smut fungus teliospore suspension with the concentration of 5 * 10 < 6 > spores / m L is prepared from smut fungus teliospore with the germination rate of 90% or above. A medical injector is adopted to inject about 25 microliters of smut fungus teliospore (the concentration is 5 * 10 < 6 > spores / mL) suspension into each sugarcane bud of a plant, and about 90 buds are inoculated to each sugarcane material. After inoculation, the sugarcane material is still planted in a greenhouse and normally managed, the morbidity condition of sugarcane smut is investigated regularly, the total morbidity plant number is counted, and the final morbidity of each treatment is calculated. Positive control is set in the test, and resistance evaluation is carried out by meeting sugarcane smut resistance grading standards.

The invention belongs to the technical field of prevention and control on crop diseases and pests, and particularly discloses a method for improving resistance of sugarcane on smut. The method comprises the following steps of before the sugarcane is planted, applying soluble silicate into silicon-lack soil, so that the content of effective silicon in the soil is 230 to 430mg / kg; uniformly mixing water and the soil, enabling the soil to react, and finally planting the sugarcane, so as to obviously reduce the generation rate of sugarcane smut, improve the resistance of the sugarcane on the smut,and promote the growth of the sugarcane. The soluble silicate can be applied to prevent and control the sugarcane shimadae and sugarcane smut. Compared with the traditional disease-resisting, seed-breeding and chemical agent-preventing and controlling methods, the method has the advantages that the safe and non-poisonous effects are realized, the green and environment-friendly effects are realized, and the operability is high; the method can be widely popularized and applied into agricultural production.

The invention belongs to the field of biopesticides, and discloses a sugarcane endophytic bacillus subtilis CGB12 strain and application thereof. The bacillus subtilis CGB12 strain is preserved in the Guangdong Microbial Culture Collection Center on March 15, 2021, the preservation number is GDMCC No: 61561, and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The bacillus subtilis CGB12 strain has a strong inhibition effect on both sugarcane smut and pyricularia grisea, has a good biological control effect, is small in environmental pollution, few in side effect and good in environmental protection effect when being used for biological control, and has a good biological application prospect.

The invention mainly relates to the technical field of planting, and discloses a sugarcane smut control method. The method comprises the steps of treatment before planting, treatment during the planting process and treatment after planting; control over smut is conducted in the whole planting process, the morbidity of the smut is decreased to 4%, and the economic income of a peasant household is increased by 11.7%; before planting is conducted, deep plowing is conducted on soil, fermented sugarcane leaves are added and soaked with a propolis solution for resisting fungi and killing pests, sugarcane sprouting is promoted, germ proliferation is reduced, and the morbidity is deceased; when planting is conducted, interplanting is conducted on garlic and sugarcane, continuous cropping damage is avoided, pathogenic microorganisms are killed, occurrence of the smut is reduced, an antibacterial agent solution is sprayed after ditching is conducted on the soil to kill the pathogenic microorganisms in the soil, and the germination rate of the sugarcane is increased by 7.3%; after planting is conducted, the temperature and humidity of a greenhouse are gradually adjusted for improving stress resistance and decreasing the occurrence rate of diseases and pests, a disease-resistant solution is periodically sprayed after germination is achieved for inhibiting the smut, and therefore the yield of the sugarcane is increased by 12.6%.

The invention relates to Bacillus subtilis HAS and an application thereof in preventing and treating sugarcane smut. The bacterial strain is named as Bacillus subtilis HAS, and is preserved in the general microorganism center of the Chinese microorganism bacterial strain preservation and administration committee, and the preservation number is CGMC No.6590. The use of the Bacillus subtilis HAS provided by the invention is the application of preventing and treating sugarcane smut; and a pot experiment shows that the Bacillus subtilis HAS has very good suppression function to sugarcane smut, the prevention effect can be 100% due to soaking seed, and the experiment shows that the bacterial strain is a biocontrol strain having potential meaning in preventing and treating sugarcane smut germs.

The present invention relates to a marker associated with resistance to smut which is a quantitative trait of sugarcane. Specifically, a marker associated with resistance to sugarcane smut, which consists of a continuous nucleic acid region existing in a region sandwiched between the nucleotide sequence shown in SEQ ID NO: 1 and the nucleotide sequence shown in SEQ ID NO: 14 or a different similar region, is provided.

The present invention relates to a marker associated with resistance to smut which is a quantitative trait of sugarcane. Specifically, a marker associated with resistance to sugarcane smut, which consists of a continuous nucleic acid region existing in a region sandwiched between the nucleotide sequence shown in SEQ ID NO: 1 and the nucleotide sequence shown in SEQ ID NO: 14 or a different similar region, is provided.

The invention mainly relates to the technical field of planting, and discloses a sugarcane smut control method. The method comprises the steps of treatment before planting, treatment during the planting process and treatment after planting; control over smut is conducted in the whole planting process, the morbidity of the smut is decreased to 4%, and the economic income of a peasant household is increased by 11.7%; before planting is conducted, deep plowing is conducted on soil, fermented sugarcane leaves are added and soaked with a propolis solution for resisting fungi and killing pests, sugarcane sprouting is promoted, germ proliferation is reduced, and the morbidity is deceased; when planting is conducted, interplanting is conducted on garlic and sugarcane, continuous cropping damage is avoided, pathogenic microorganisms are killed, occurrence of the smut is reduced, an antibacterial agent solution is sprayed after ditching is conducted on the soil to kill the pathogenic microorganisms in the soil, and the germination rate of the sugarcane is increased by 7.3%; after planting is conducted, the temperature and humidity of a greenhouse are gradually adjusted for improving stress resistance and decreasing the occurrence rate of diseases and pests, a disease-resistant solution is periodically sprayed after germination is achieved for inhibiting the smut, and therefore the yield of the sugarcane is increased by 12.6%.

The invention belongs to the field of biotechnology, and specifically discloses a sugarcane smut SCoT-PCR (polymerase chain reaction) specific primer screening and an application. The primer sequence is shown as SEQ ID NO:1 to 10. Ten primers are used for amplifying ten sugarcane smut matching strains from different areas and hosts, totally, 86 bands are amplified, averagely, each primer amplifies 8.6 bands, the polymorphic rate is 59.3%. The screened specific primer has relatively high polymorphic detection efficiency, and can be used for genetic diversity analysis on sugarcane smut; and theoretical direction for selection of disease-resistant varieties of sugarcane is provided.

The invention relates to a pesticide fertilizer containing sugarcane ash as well as a preparation method and application of the pesticide fertilizer. The pesticide fertilizer containing the sugarcane ash is prepared from a first active component, a second active component, the sugarcane ash, a biological fertilizer and a pesticide fertilizer component. Oxalis corniculata powder and ageratum conyzoides powder are added into the pesticide fertilizer containing the sugarcane ash according to the proportion, the morbidity of the sugarcane smut can be obviously reduced, and the purpose of effectively preventing the sugarcane smut is achieved. Boiler combustion waste sugarcane ash is mixed with molasses alcohol fermentation liquor, sugar refinery filter mud and other components according to a certain weight ratio to prepare a biological fertilizer, waste generated after sugarcane squeezing is fully utilized, organic and mineral nutrient elements of sugarcane are effectively utilized, and substances absorbed by the sugarcanes in the growth period are circulated to the soil environment again, sufficient absorbable nutrients are provided for subsequent growth of the sugarcanes, soil acidification and hardening caused by excessive application of chemical fertilizers are greatly reduced, and meanwhile the yield and quality of crops are effectively improved.

The invention relates to the field of biotechnology and particularly discloses an LAMP (loop-mediated isothermal amplification) primer group for quickly detecting sporisorium scitamineum, a kit and a detection method thereof. In the invention, a primer group F3 / B3 and FIP / BIP is designed according to the ribosome gene internal transcribed spacer (ITS) sequence of sporisorium scitamineum, the sporisorium scitamineum is detected by the primer group or a kit containing the primer group through LAMP and the detection has the advantages of low cost, easy operation, simplicity, high speed, visual detection result, judgment with naked eyes, high sensitivity, high specificity and the like; and the LAMP primer group, the kit and the detection method disclosed by the invention are applicable to the sugarcane introduction quarantine in the basic-level departments, quality detection on detoxified seedlings, field early detection of culmicolous smut and the like, and has extensive and practical application value.