27 results about "Sugarcane smut" patented technology

The invention relates to the field of agriculture, and discloses a method for evaluating sugarcane smut based on an unmanned aerial vehicle image, which comprises the following steps: data acquisition: planning a route by using an unmanned aerial vehicle carrying a multispectral camera, a hyperspectral camera and an RGB (Red, Green and Blue) camera, so that the unmanned aerial vehicle acquires image data of sugarcane; preprocessing: sequentially carrying out geometric correction, radiation correction and image fusion processing on the image data to obtain preprocessed data; feature fusion: constructing a fusion model including spectral feature extraction, visual feature extraction and spatial-temporal feature fusion according to the feature expression of the sugarcane smut; and model generalization: collecting multi-source images and disease investigation data according to the actual condition of the sugarcane. The method comprises the following steps: performing data acquisition through an unmanned aerial vehicle, obtaining multi-dimensional image data in a sugarcane growth critical period, performing preprocessing, constructing a fusion model including spectral feature extraction, visual feature extraction and spatial-temporal feature fusion, performing fine adjustment and optimization in combination with a transfer learning strategy, and performing real-time analysis on a disease detection result.

The invention provides a sustained-release and controlled-release sugarcane bacteriostatic pesticide-fertilizer particle and a preparation method and application thereof, and belongs to the technical field of agricultural sustained-release and controlled-release fertilizer.The pesticide-fertilizer particle is coated with chitosan-sodium alginate double-network hydrogel, has the pH intelligent response characteristic and can adjust the nutrient and bactericide release rate in a self-adaptive mode according to the soil pH change; oxathiapiprolin and pyraclostrobin are compounded, so that sugarcane smut, maculopathy and rust disease are prevented and treated in a broad-spectrum and high-efficiency manner; a biomass charcoal-bentonite-zeolite molecular sieve composite carrier and a bacillus amyloliquefaciens composite flora are introduced, so that the micro-ecology of the soil is improved; a three-layer coating structure simplifies the preparation process, the production cost is reduced, and field tests show that the pesticide-fertilizer particles provided by the invention can increase the yield of sugarcane by 12-18%, increase the sugar content by 0.5-1.2%, reduce the disease incidence by 70-80%, increase the nutrient utilization rate by 20-35%, and increase the soil organic matter content by 0.6-4.6 g / kg.

The invention relates to the technical field of sugarcane smut resistance, and provides a molecular marking method for sugarcane smut resistance, which comprises a sample preparation unit, a double-sugarcane area inoculation identification unit, a whole genome association analysis unit, an SNP (Single Nucleotide Polymorphism) marker development unit, a marker verification unit and a result application unit, the output end of the sample preparation unit is connected with the input end of the double-sugarcane-area inoculation identification unit through a sample transmission channel, the double-sugarcane-area inoculation identification unit outputs phenotypic data to the whole-genome association analysis unit, and the whole-genome association analysis unit outputs remarkably associated SNP sites to the SNP marker development unit; and the SNP marker development unit outputs a specific primer to the marker verification unit. The molecular marker provided by the invention is based on the correlation between the candidate gene of the site and the known disease-resistant pathway of the plant, and the MYB transcription factor can improve or reduce the resistance level of the plant to pathogenic bacteria by regulating the synthesis of secondary metabolites, transduction of plant hormone signals, expression of defensive genes and the like.

The invention discloses application of brassinolide in improving resistance of sugarcane to smut. According to the method, brassinolide is sprayed on leaf surfaces, so that the brassinolide can improve the resistance of sugarcane to smut caused by sugarcane smut, the morbidity of sugarcane plants infected by sugarcane smut is remarkably reduced, and the influence of sugarcane smut on the yield and quality of sugarcane is reduced. Therefore, the invention provides application of brassinolide in improving or preparing a product for improving the resistance of sugarcane to smut. The method is beneficial to green control of sugarcane smut, and is simple to operate and good in feasibility.

The invention discloses an application of a pathogenic factor of sugarcane smut in regulation and control of pathogenicity of sugarcane smut. The amino acid sequence of the pathogenic protein SsCyp64 is as shown in SEQ ID NO: 2, and the coding amino acid sequence of the pathogenic gene SsCYP64 is as shown in SEQ ID NO: 1. According to the invention, SsCYP64 is knocked out from a sugarcane smut wild type through a PEG-mediated protoplast transformation method, so that a knockout mutant is obtained; and respectively back-supplementing the gene in the knockout mutant to obtain a back-supplemented strain. The SsCYP64 has the advantages that the SsCYP64 knockout mutant has the advantages that the capability of forming double mycelia by sexual coordination is weakened, the sensitivity to H2O2 and the SDS stress tolerance are increased, and the pathogenicity is reduced, so that the SsCYP64 plays an important role in regulating the sexual coordination, the oxidation resistance, the SDS tolerance and the pathogenicity of the sugarcane smut. The invention provides a target gene for developing effective bactericides.

The utility model relates to the technical field of sugarcane smut detection, in particular to a sugarcane smut detection device which comprises a detection box body, the device also comprises a rotating handle, a driving shaft, a detection frame, a driven shaft, a fixed plate, a driven clamping plate, a spring and a driving clamping plate. The center of the right side of the detection box body is provided with the rotating handle. Sugarcane leaves or stalks are placed in the detection frame, then the driving clamping plate is lifted upwards, one end of each sugarcane leaf or stalk is placed between the driving clamping plate and the driven clamping plate, then the driving clamping plate is loosened, and the driving clamping plate is pushed to be matched with the driven clamping plate through the characteristics of the spring to clamp one end of each sugarcane leaf or stalk. When the two ends of the sugarcane leaf or stalk are clamped, the driving shaft is rotated through the rotating handle, the driving shaft is matched with the driven shaft to drive the detection frame to rotate, the detection frame drives the clamped sugarcane leaf or stalk to rotate, and the comprehensiveness of the sugarcane leaf or stalk during detection is guaranteed.

The invention relates to the technical field of genetic engineering, in particular to a gene MEU26 related to sexual coordination and pathopoiesis of sugarcane sporosmut, protein and application of the gene MEU26. The gene MEU26 is as shown in SEQ ID NO. 1. A delta MEU26 mutant strain is constructed through a PEG-mediated protoplast transformation method, a backfill strain is constructed through an ATMT method, it is found that the sexual cooperation ability and pathogenicity of the delta MEU26 mutant strain are affected, the sexual cooperation ability and pathogenicity of the backfill strain are basically recovered, and it is indicated that the MEU26 gene participates in regulation and control of the sexual cooperation and pathogenicity of the Saccharospora canadensis Saccharospora canadensis Saccharospora canadensis Saccharospora canadensis Saccharospora canadensis Saccharospora canadensis. A foundation is provided for screening and developing products for reducing pathogenicity of sugarcane sporoderm smut, and a new target is provided for researching and developing agents for preventing and treating sugarcane smut and a novel efficient disease prevention and control technology.

The present application relates to the technical field of sugarcane smut prevention and treatment, and particularly relates to a microbial fertilizer, a preparation method thereof and application thereof in prevention and treatment of smut. The microbial fertilizer comprises, by weight, 40-60 parts of cow dung, 2-4 parts of corn straw biochar, 4-6 parts of mulberry branch and 8-14 parts of mixed bacterial agent. The mixed bacterial agent is composed of Bacillus subtilis X1, Bacillus altitudinis X2, Bacillus cereus X3, Trichoderma harzianum Z1 and Trichoderma longibrachiatum Z2. The microbial fertilizer has a positive promoting effect on sugarcane and can significantly stimulate the self-defense response of sugarcane.

The invention discloses a pseudomonas bainite 2143-2 strain and an application of the pseudomonas bainite 2143-2 strain. The pseudomonas bainii 2143-2 strain disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center on September 2, 2025, and the preservation number is GDMCC No: 66909. The pseudomonas bainii 2143-2 strain disclosed by the invention has the advantages that the pseudomonas bainii 2143-2 strain is preserved in the Guangdong Microbial Culture Collection Center on September 2, 2025; the pseudomonas bainite 2143-2 strain not only can effectively inhibit the growth of sugarcane smut and remarkably reduce the morbidity of sugarcane smut, but also can effectively inhibit the growth of sugarcane leaf spot bacteria, sugarcane red rot bacteria, sugarcane brown streak bacteria, sugarcane ring spot bacteria and sugarcane tip rot bacteria, and the antibacterial rate reaches 65% or above. The pseudomonas bainite 2143-2 strain can be used for biological prevention and control of sugarcane smut, sugarcane leaf spot, sugarcane red rot, sugarcane brown streak, sugarcane ring spot and sugarcane tip rot, and development of sugarcane fungal disease prevention and control products is facilitated.

The invention discloses an application of a PRM1 gene in prevention and control of sugarcane smut, and the gene encodes a pheromone regulated multi-transmembrane protein which is a key factor for regulating sexual coordination and pathogenicity of pathogenic bacteria. Experiments show that inhibition or knockout of the PRM1 gene can significantly weaken the sexual cooperation ability of pathogenic bacteria, hinder the formation of binuclear hyphae and cause the reduction of the pathogenicity of the binuclear hyphae. In a molecular mechanism, PRM1 plays a role by influencing expression of a key gene of a pheromone signaling pathway. On the basis, the PRM1 gene and the encoding protein thereof are determined as a new target for preventing and treating the sugarcane smut, and can be used for developing novel green prevention and control technologies and products such as RNA interference preparations, specific inhibitors, gene editing breeding and engineering microorganisms, and a brand-new molecular basis and solution are provided for effectively controlling the sugarcane smut.

The invention discloses a SsTEM1 gene and application of the SsTEM1 gene in prevention and treatment of sugarcane smut, the nucleotide sequence of the SsTEM1 gene is shown as SEQ ID NO: 2, and a GTP binding protein containing an Spg1 structural domain is encoded. The key regulation effect of the SsTEM1 gene in the sexual coordination and pathopoiesis process of pathogenic bacteria is identified and confirmed for the first time: the SsTEM1 gene is highly expressed in a hypha stage; after the gene is knocked out, the cell wall integrity of the pathogenic bacteria is damaged, the sexual cooperation capability is obviously weakened, and the pathogenicity severely declines and even completely loses. Based on this, the present invention protects the gene and the protein sequence, and provides a control method using the gene as a target, including the control of diseases using a substance capable of inhibiting the expression of the gene or the activity of the protein, and a detection method and a disease-resistant plant cultivation method based on the gene. The invention provides a brand new molecular target and theoretical basis for developing an efficient and specific sugarcane smut green prevention and control technology.

The invention discloses a Burkholderia diphtheria 2143-6 strain and an application of the Burkholderia diphtheria 2143-6 strain. The Burkholderia diphtheria 2143-6 strain disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center on September 2, 2025, and the preservation number of the Burkholderia diphtheria 2143-6 strain is GDMCC No: 66910. The strain 2143-6 can inhibit the growth of sugarcane smut and reduce the morbidity of sugarcane smut, and can be used for preventing and treating sugarcane smut and reducing the adverse effect of sugarcane smut on the yield and the hammer sugar degree of sugarcane. Besides, the 2143-6 bacterial strain also has an inhibition effect on sugarcane leaf spot bacteria, sugarcane red rot bacteria, sugarcane brown streak bacteria and sugarcane ring spot bacteria, can be used for developing important products for preventing and treating sugarcane fungal diseases, and is beneficial to guaranteeing the yield and quality of sugarcanes.

The application provides application of prunol in prevention and treatment of sugarcane smut. It is verified through experiments that prunol can inhibit growth of haploid strains JG35 and JG36 of sugarcane smut fungus, and the inhibition on sexual compatibility of the two strains is more prominent, which provides a new way for green prevention and control of sugarcane smut. The method provided by the application has the advantages of green and no residue, simple operation, stable prevention and control effect and the like, and has a wide application prospect.

This application relates to a strain of *Pseudomonas lundense* and its applications. The present invention isolates a strain of *Pseudomonas lundense* from the root soil of rice plants in a rice cultivation base. This strain is effective against rice basal rot pathogens (…). Dickeya oryzae ), potato soft rot fungus ( Dickeya dadantii ), and Taro soft rot fungus ( Dickeya fangzhongdai It has a good control effect on sugarcane smut ( ) Sporisorium scitamineum ), rice blast fungus ( Magnaporthe oryzae ), Neodatura spp. Neoscytalidium dimidiatum ), neatly arranged small sclerotia ( Sclerotium rolfsii ), Lithocarpus ( Calonectria ), Black spores ( Nigrospora lacticoloni a) Ralstonia solanacearum ( Ralstonia solanacearum Xanthomonas aeruginosa (), Xanthomonas campestris pv. Campestris ) and citrus canker pathogen ( Xanthomonas citri subsp. citri It also has a good control effect. Therefore, it can be widely used in the control of plant diseases, including rice basal rot.

This invention relates to *Burkholderia gladioli* and its application in the preparation of products for controlling fungal diseases in sugarcane, belonging to the field of biological control technology. The *Burkholderia gladioli* of this invention is *Burkholderia gladioli*. Burkholderia gladioli Strain B1, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37420, is effective in controlling fungal diseases such as sugarcane smut, sugarcane top rot, sugarcane brown streak, and sugarcane red rot. It also possesses phosphorus-solubilizing and growth-promoting properties, thereby effectively promoting nutrient absorption and plant growth in crops.

This invention discloses a pathogenic factor of sugarcane smut and its application. This invention discovers... SsPHACA Application in regulating sexual mating, SDS tolerance, and pathogenicity of *Ustilago maydis*. This invention utilizes the principle of homologous recombination, employing a PEG-mediated protoplast transformation method to... SsPHACA Knockout and replacement were performed, resulting in knockout mutants and replacement strains. The results showed... SsPHACA Knockout resulted in a reduced ability of *Ustilago canescens* to form dimycelia through sexual mating, increased susceptibility to SDS, and decreased pathogenicity, indicating... SsPHACA This gene plays a crucial role in regulating the sexual mating, SDS tolerance, and pathogenicity of *Ustilago maydis*. This invention provides a novel target gene for the development of effective fungicides.

The invention discloses a method for improving the synergistic nitrogen fixation capacity of acid-aluminum-resistant sugarcane and microorganisms, and relates to the technical field of agricultural planting, and the method comprises the following steps: irrigating roots by using a nitrogen-fixing bacterial agent in the tillering stage of the sugarcane; the nitrogen-fixing bacterial agent is prepared from the following raw material components: 2 to 3 parts of pseudomonas stutzeri, 3 to 5 parts of brown nitrogen-fixing bacteria, 1 to 2 parts of pantoea agglomerans and 0.1 to 2 parts of maleic anhydride. By optimizing the composition of the nitrogen-fixing bacteria, the synergistic nitrogen-fixing effect of the sugarcane and microorganisms is promoted, the combination of the nitrogen-fixing bacteria can perform metabolic activity in soil and sugarcane seed stems, saccharides, amino acids, vitamins, nitrogen elements, bioactive substances and the like generated by metabolism can be absorbed by the sugarcane seed stems, growth of roots of the sugarcane seed stems can be promoted, and the growth rate of the sugarcane seed stems is increased. The acid-aluminum resistance of the sugarcane is improved; pseudomonas stutzeri and azotobacter brown produce acid to chelate aluminum in soil, the content of active aluminum in the soil is reduced, and pantoea agglomerans are cooperated to effectively prevent and treat sugarcane smut.

The invention belongs to the technical field of biological prevention and control of plant diseases, and particularly relates to a biological source rhizosphere metabolite phthalic acid and application thereof in prevention and control of sugarcane smut. The invention relates to an application of a biological source rhizosphere metabolite in prevention and control of sugarcane smut. The biological source rhizosphere metabolite is phthalic acid. The key rhizosphere metabolite phthalic acid with efficient and targeted bacteriostatic activity is screened and identified from disease-inhibiting soil, safe and efficient prevention and control of sugarcane smut are achieved by targeted inhibition of germination of pathogenic bacteria chlamydospore, and the key rhizosphere metabolite phthalic acid can be developed as a biological source pesticide and has broad application prospects. And the method has great significance on pesticide reduction, synergism and green development of agriculture.

The invention belongs to the technical field of sugarcane germ detection, and particularly relates to a dual-mode detection method for sugarcane smut fungi. According to the dual-mode detection method for the sugarcane smut fungi, a fluorescence-naked eye reading dual-mode lateral chromatography platform for detecting the sugarcane smut fungi genes is developed by utilizing a composite material RuPd (at) vCDs and based on a rolling circle amplification and Cas12a active RCA-Cas platform. The method depends on a target chain as a primer, triggers an RCA process to generate a DNA product, and then activates the trans-cleavage activity of Cas12a, so that DNAC is catalytically cleaved. CDs is used as a luminescent probe for detecting a fluorescence signal, and RuPd bimetallic can catalytically decompose hydrogen peroxide by using own catalase-like activity, so that an alginate-tyramine conjugate forms gel for measuring a flow distance. The dual-mode detection realizes ultra-sensitive and rapid detection of the smut smut of sugarcane, and shows a wide application prospect.

The application discloses a double-detection self-powered sensor for detecting sugarcane diseases, application and a detection method thereof, and comprises a biological anode based on nucleic acid amplification product M, a biological cathode based on Au@MB and a self-powered sensor constructed by an electrolyte; wherein the electrolyte comprises glucose, methylene blue PBS buffer and a photothermal test solution. The self-powered sensor can efficiently amplify the nucleic acid of the pathogen of sugarcane smut and shoot rot through a nucleic acid amplification technology, so that the sensor can detect the pathogen at a low concentration, and the missing detection rate is effectively reduced. Meanwhile, multi-mode synergistic detection (electrochemistry, colorimetry and photothermal) further improves the accuracy and specificity of detection, avoids the misjudgment caused by signal cross interference in the traditional method, and ensures the reliability of the detection result.

The invention discloses a multi-mode sugarcane smut detection platform based on a space separation strategy and application of the multi-mode sugarcane smut detection platform, and relates to the technical field of biosensing and chemical analysis. The invention provides a spatially separated multi-mode sensing platform aiming at the problems that traditional single signal detection is easily interfered to cause false positive or false negative, and common multi-mode detection may cause signal crosstalk and the like. The platform is creatively integrated with three functional modules which are separated in space, namely a folded double-chain DNA modified magnetic bead module, a Y-type three-chain DNA modified ITO electrode module and a CRISPR / Cas12a functionalized DNA hydrogel module, wherein the three functional modules are the folded double-chain DNA modified magnetic bead module, the Y-type three-chain DNA modified ITO electrode module and the CRISPR / Cas12a functionalized DNA hydrogel module. After a target gene is subjected to specific recognition and amplification, independent output of three signals of photoelectrochemistry, electrochemistry and fluorescence is realized through cooperation of the three signals. According to the method, false positive is effectively inhibited through three-mode signal cross validation, crosstalk between detection modes is eliminated in combination with a space separation strategy, and the efficient and accurate multi-mode detection method for early diagnosis and field monitoring of sugarcane smut is provided.

The invention discloses application of an RWTD1 gene in prevention and control of sugarcane smut. The RWTD1 gene is a key factor for regulating sexual coordination of pathogenic bacteria and development of pathogenic symptoms (black whips). Through functional verification, pathogenic bacteria cannot complete the whole life history after RWTD1 gene deletion: even if the pathogenic bacteria can infect hosts, plants cannot be induced to form black whips and generate teliospore, so that a disease transmission chain is cut off. On the basis, the RWTD1 gene, the encoding protein thereof or a related regulation and control sequence are used as prevention and control targets, various application ways including RNA interference, gene editing, chemical inhibition and disease-resistant breeding are provided, and a core molecular basis and a brand new solution are provided for developing a new efficient, accurate and environment-friendly sugarcane smut prevention and control technology.

The invention discloses a dual-detection self-energized sensor for detecting sugarcane diseases, application and a detection method thereof. The dual-detection self-energized sensor comprises a biological anode based on a nucleic acid amplification product M, a biological cathode based on Au (at) MB and a self-energized sensor constructed by electrolyte, wherein the electrolyte comprises glucose, a PBS (Phosphate Buffer Solution) of methylene blue and a photo-thermal test solution. According to the self-energized sensor, nucleic acid of pathogens of sugarcane smut and pokkah top rot is efficiently amplified through a nucleic acid amplification technology, so that the sensor can detect the pathogens with low concentration, and the omission ratio is effectively reduced. Meanwhile, multi-mode cooperative detection (electrochemistry, colorimetry and photo-thermal) further improves the accuracy and specificity of detection, avoids misjudgment caused by signal cross interference in a traditional method, and ensures the reliability of a detection result.

The present invention belongs to the field of microbial technology, and specifically relates to an antagonistic strain Penicillium crustosum LZ31 for efficiently preventing and controlling sugarcane smut and its application. An antagonistic strain LZ31, characterized in that the taxonomic name of the antagonistic strain LZ31 is Penicillium crustosum, which was deposited in the Guangdong Microbiological Culture Collection Center on August 23, 2024, with a deposit number of GDMCC No: 65052. The present invention screens strains that inhibit the growth of sugarcane smut fungi to obtain a fungal strain LZ31 with strong resistance, and then molecular biological identification shows that strain LZ31 is Penicillium crustosum. Through potted plant experiments, it was found that strain LZ31 can not only efficiently prevent and control sugarcane smut, but also promote the growth of sugarcane plants and the accumulation of biomass, which can provide support for the development of microbial preparations and bacterial fertilizers for preventing and controlling sugarcane smut, and has important practical value for the green and high-quality development of the sugarcane industry.

The application discloses application of a pathogenic factor of a sugarcane smut fungus in regulating pathogenicity of the sugarcane smut fungus. An amino acid sequence of the pathogenic protein SsCyp64 is shown as SEQ ID NO: 2, and a pathogenic gene SsCYP64 coding the amino acid sequence is shown as SEQ ID NO: 1. The application obtains a knockout mutant by a PEG-mediated protoplast transformation method in a wild type of the sugarcane smut fungus SsCYP64 , and obtains a back complement strain by respectively back complementing the gene in the knockout mutant. It is found that the knockout mutant has weakened ability of forming a double mycelium by sexual cooperation, increased sensitivity to H2O2 and SDS stress tolerance, and decreased pathogenicity, which indicates that SsCYP64 the pathogenic factor plays an important role in regulating sexual cooperation, oxidation resistance, SDS tolerance and pathogenicity of the sugarcane smut fungus. The application provides a target gene for developing an effective fungicide.

The invention discloses a sugarcane smut pathogenic factor and application thereof. The invention discovers application of SsPHACA in regulation and control of sexual cooperation, SDS (sodium dodecyl sulfate) tolerance and pathogenicity of sugarcane smut. According to the invention, based on the principle of homologous recombination, SsPHACA is knocked out and back-supplemented through a PEG-mediated DNA double-fragment protoplast transformation method, so that a knockout mutant and a back-supplemented strain are obtained. Results show that the SsPHACA knockout results in weakening of the ability of the sugarcane smut to form double mycelia through sexual cooperation, increase of SDS sensitivity and reduction of pathogenicity, and show that the SsPHACA plays a key role in regulating the sexual cooperation of the sugarcane smut, the SDS tolerance and the pathogenicity. The invention provides a new target gene for developing effective bactericides.

The invention relates to the technical field of microorganisms, and discloses application of an antibacterial peptide AG-AFP, and the amino acid sequence of the antibacterial peptide AG-AFP is as shown in SEQ ID NO: 1. The invention provides an antibacterial peptide AG-AFP capable of inhibiting sugarcane smut, and the antibacterial peptide AG-AFP can inhibit the growth of haploid yeast spores and binuclear hyphae of sugarcane smut pathogenic bacteria (sugarcane smut), so that the sugarcane smut can be effectively prevented and treated; besides, the antibacterial peptide AG-AFP is coded by a gene and can be used for subsequent preparation of sugarcane transgenic breeding capable of resisting smut, and a new direction is provided for prevention and treatment of sugarcane smut.