41 results about "Sugarcane smut" patented technology

The invention discloses a method for inducing sugarcane to resist sugarcane smut, and belongs to the technical field of sugarcane disease resistance. The method disclosed by the invention comprises the following steps: (1) obtaining sugarcane smut Pep1 gene knockout mutants PepMAT-1 and PepMAT-2; (2) culture of a sugarcane smut Pep1 gene knockout mutant: activating PepMAT-1 and PepMAT-2, and then inoculating the activated PepMAT-1 and PepMAT-2 to a YePS liquid culture medium for shake cultivation; and (3) inducing sugarcane to resist smut: after shake cultivation, mixing the PepMAT-1 and the PepMAT-2, and inoculating the mixture on sugarcane seedlings. The method can rapidly and efficiently induce sugarcane to resist sugarcane smut, and provides technical support for prevention and treatment of sugarcane smut.

The invention provides a fungus for antagonizing sexual cooperation of smut smut of sugarcane and application thereof, and belongs to the technical field of biological prevention and control. The blue vibrio T-15 provided by the invention is a fungus separated from soil and can be better colonized in the soil, and it is found for the first time that the fungus can effectively antagonize sugarcane smut fungi for sexual cooperation to enable the sugarcane smut fungi to lose pathogenicity, can be applied to prevention and treatment of sugarcane smut, and has potential application value; the blue virens T-15 has an inhibition effect on the infection of the sugarcane smut fungi; the blue virens T-15 also has certain growth promoting potential and has the capability of producing siderophores; the invention provides a new fungus resource for biological control of sugarcane smut.

The invention discloses a self-powered sensor for detecting sugarcane smut pathogenic bacteria and a preparation method thereof, the self-powered biosensor comprises an anode, a cathode and an electrolyte, the anode is an Au / N-GDY / GOD / BSA biological anode, the cathode is an Au / N-GDY / DNA chain biosensor, and the electrolyte comprises glucose, [Ru (NH3) 6] 3 + and a PBS buffer solution. The sensor provided by the invention is used for portable detection of pathogenic bacteria of sugarcane smut, and quantitative analysis of a target analyte is realized through change of power output of the enzyme biological fuel cell. The target object can be simply, quickly, sensitively and efficiently detected.

The invention discloses a Brachybacterium stolonifer T-12, a microbial agent and application, and belongs to the technical field of biological prevention and control. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 9, 2024, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.41659. The Talaromyces Stokes T-12 is a fungus separated from soil and can be well colonized in the soil, it is found for the first time that the fungus has an obvious inhibition effect on sugarcane smut, sugarcane brown streak and sugarcane pokkah boeng for the first time, and the Talaromyces Stokes T-12 can be applied to prevention and treatment of sugarcane smut, sugarcane brown streak and sugarcane pokkah boeng. Particularly, the compound has an excellent prevention and treatment effect on sugarcane smut and has potential application value; and meanwhile, the strain also has certain growth promoting potential, has the capability of producing siderophores, and has wide popularization and application prospects.

The invention discloses a sugarcane smut dual-mode sensing platform and application thereof, and relates to the technical field of biological detection and chemical analysis. The sensing platform comprises a three-chain DNA (Deoxyribose Nucleic Acid) and a CdTe QDs-NH2-F chain, the triplex DNA is a double strand consisting of an MB-A chain and an FAM-B chain; the MB-A chain is an A chain connected with magnetic beads; the FAM-B chain is a B chain which modifies a fluorophore and can form a hairpin structure; the CdTe QDs-NH2-F chain is an F chain which is aminated and is connected with the cadmium telluride quantum dots; a part of base sequence of the chain A is complementary to a base sequence of a sugarcane smut pathogenic bacterium gene; the base sequence of the chain F is complementary to the partial base sequence of the chain A, and the partial base sequence of the chain F is the same as the partial base sequence of the sugarcane smut pathogenic bacterium gene and the partial base sequence of the chain B. The sensing platform can sensitively amplify and efficiently amplify the genes of the pathogenic bacteria of the sugarcane smut, and can respectively obtain independent detection results of a photoelectrochemical detection mode and a fluorescence detection mode, so that the sensitivity and the accuracy of the detection results are improved.

The invention relates to the field of agriculture, and discloses a method for evaluating sugarcane smut based on an unmanned aerial vehicle image, which comprises the following steps: data acquisition: planning a route by using an unmanned aerial vehicle carrying a multispectral camera, a hyperspectral camera and an RGB (Red, Green and Blue) camera, so that the unmanned aerial vehicle acquires image data of sugarcane; preprocessing: sequentially carrying out geometric correction, radiation correction and image fusion processing on the image data to obtain preprocessed data; feature fusion: constructing a fusion model including spectral feature extraction, visual feature extraction and spatial-temporal feature fusion according to the feature expression of the sugarcane smut; and model generalization: collecting multi-source images and disease investigation data according to the actual condition of the sugarcane. The method comprises the following steps: performing data acquisition through an unmanned aerial vehicle, obtaining multi-dimensional image data in a sugarcane growth critical period, performing preprocessing, constructing a fusion model including spectral feature extraction, visual feature extraction and spatial-temporal feature fusion, performing fine adjustment and optimization in combination with a transfer learning strategy, and performing real-time analysis on a disease detection result.

The invention provides a sustained-release and controlled-release sugarcane bacteriostatic pesticide-fertilizer particle and a preparation method and application thereof, and belongs to the technical field of agricultural sustained-release and controlled-release fertilizer.The pesticide-fertilizer particle is coated with chitosan-sodium alginate double-network hydrogel, has the pH intelligent response characteristic and can adjust the nutrient and bactericide release rate in a self-adaptive mode according to the soil pH change; oxathiapiprolin and pyraclostrobin are compounded, so that sugarcane smut, maculopathy and rust disease are prevented and treated in a broad-spectrum and high-efficiency manner; a biomass charcoal-bentonite-zeolite molecular sieve composite carrier and a bacillus amyloliquefaciens composite flora are introduced, so that the micro-ecology of the soil is improved; a three-layer coating structure simplifies the preparation process, the production cost is reduced, and field tests show that the pesticide-fertilizer particles provided by the invention can increase the yield of sugarcane by 12-18%, increase the sugar content by 0.5-1.2%, reduce the disease incidence by 70-80%, increase the nutrient utilization rate by 20-35%, and increase the soil organic matter content by 0.6-4.6 g / kg.

The invention belongs to the technical field of agricultural equipment, and discloses a sugarcane smut inoculation device which comprises an inoculation liquid box and further comprises a sugarcane box, the sugarcane box is arranged in the inoculation liquid box through a fixing mechanism, and an adjusting mechanism is arranged on the inner wall of the sugarcane box; the driving mechanism is arranged in the inner wall of the inoculation liquid box; the filter screen is connected into the inner wall of the inoculation liquid box in a sliding manner; the stirring rod is rotationally connected to the inner wall of the bottom end of the inoculation liquid box; wherein the driving mechanism comprises a motor, and an output shaft of the motor is provided with a limiting assembly; through cooperation of the limiting assembly, the external threaded rod and other structures, the motor can drive the external threaded rod or the synchronizing wheel A to rotate to drive the filter screen to move up and down or drive the stirring rod to rotate, impurities in the filter screen can be conveniently cleaned, the situation that inoculation liquid precipitates and influences quality can be avoided, operation is easy, one set of motor can achieve two functions, and cost is low.

The invention relates to the technical field of sugarcane smut resistance, and provides a molecular marking method for sugarcane smut resistance, which comprises a sample preparation unit, a double-sugarcane area inoculation identification unit, a whole genome association analysis unit, an SNP (Single Nucleotide Polymorphism) marker development unit, a marker verification unit and a result application unit, the output end of the sample preparation unit is connected with the input end of the double-sugarcane-area inoculation identification unit through a sample transmission channel, the double-sugarcane-area inoculation identification unit outputs phenotypic data to the whole-genome association analysis unit, and the whole-genome association analysis unit outputs remarkably associated SNP sites to the SNP marker development unit; and the SNP marker development unit outputs a specific primer to the marker verification unit. The molecular marker provided by the invention is based on the correlation between the candidate gene of the site and the known disease-resistant pathway of the plant, and the MYB transcription factor can improve or reduce the resistance level of the plant to pathogenic bacteria by regulating the synthesis of secondary metabolites, transduction of plant hormone signals, expression of defensive genes and the like.

The present invention provides a fungus that antagonizes the sexual mating of sugarcane smut fungi and its application, belonging to the field of biological control technology. The dark green basket fungus T-15 provided by the present invention is a fungus isolated from soil, which can be well colonized in the soil. The present invention first discovered that the fungus can effectively antagonize the sexual mating of sugarcane smut fungi, causing it to lose its pathogenicity, and can be used to prevent and control sugarcane smut, with potential application value; the dark green basket fungus T-15 has an inhibitory effect on the infection of sugarcane smut fungi; the dark green basket fungus T-15 also has a certain growth-promoting potential and has the ability to produce iron carriers; the present invention provides a new fungus resource for the biological control of sugarcane smut.

The invention discloses application of brassinolide in improving resistance of sugarcane to smut. According to the method, brassinolide is sprayed on leaf surfaces, so that the brassinolide can improve the resistance of sugarcane to smut caused by sugarcane smut, the morbidity of sugarcane plants infected by sugarcane smut is remarkably reduced, and the influence of sugarcane smut on the yield and quality of sugarcane is reduced. Therefore, the invention provides application of brassinolide in improving or preparing a product for improving the resistance of sugarcane to smut. The method is beneficial to green control of sugarcane smut, and is simple to operate and good in feasibility.

The invention discloses an application of a pathogenic factor of sugarcane smut in regulation and control of pathogenicity of sugarcane smut. The amino acid sequence of the pathogenic protein SsCyp64 is as shown in SEQ ID NO: 2, and the coding amino acid sequence of the pathogenic gene SsCYP64 is as shown in SEQ ID NO: 1. According to the invention, SsCYP64 is knocked out from a sugarcane smut wild type through a PEG-mediated protoplast transformation method, so that a knockout mutant is obtained; and respectively back-supplementing the gene in the knockout mutant to obtain a back-supplemented strain. The SsCYP64 has the advantages that the SsCYP64 knockout mutant has the advantages that the capability of forming double mycelia by sexual coordination is weakened, the sensitivity to H2O2 and the SDS stress tolerance are increased, and the pathogenicity is reduced, so that the SsCYP64 plays an important role in regulating the sexual coordination, the oxidation resistance, the SDS tolerance and the pathogenicity of the sugarcane smut. The invention provides a target gene for developing effective bactericides.

The present invention discloses a sugarcane lignin-related gene ScDIR11 and its application in smut-resistant sugarcane breeding. The inventors found through studying the sugarcane lignin synthesis-related gene ScDIR11 that overexpressing the ScDIR11 gene of sugarcane itself can lead to an increase in the lignin content of transgenic sugarcane, and the transgenic sugarcane infected with sugarcane whip smut showed that its smut incidence rate was significantly reduced, and the content of sugarcane whip smut in the transgenic plants decreased. Based on this, the inventors established a method for obtaining highly smut-resistant sugarcane, and a plasmid containing the sugarcane ScDIR11 gene was genetically transformed into sugarcane through Agrobacterium tumefaciens-mediated method to obtain highly smut-resistant sugarcane materials. Therefore, the present invention is conducive to clarifying the disease resistance mechanism of sugarcane to sugarcane whip smut by cloning the gene ScDIR11, and is applied to the directional molecular breeding of highly disease-resistant sugarcane through genetic engineering means, providing technical support for the use of the sugarcane ScDIR11 gene to prevent and control sugarcane smut.

The utility model relates to the technical field of sugarcane smut detection, in particular to a sugarcane smut detection device which comprises a detection box body, the device also comprises a rotating handle, a driving shaft, a detection frame, a driven shaft, a fixed plate, a driven clamping plate, a spring and a driving clamping plate. The center of the right side of the detection box body is provided with the rotating handle. Sugarcane leaves or stalks are placed in the detection frame, then the driving clamping plate is lifted upwards, one end of each sugarcane leaf or stalk is placed between the driving clamping plate and the driven clamping plate, then the driving clamping plate is loosened, and the driving clamping plate is pushed to be matched with the driven clamping plate through the characteristics of the spring to clamp one end of each sugarcane leaf or stalk. When the two ends of the sugarcane leaf or stalk are clamped, the driving shaft is rotated through the rotating handle, the driving shaft is matched with the driven shaft to drive the detection frame to rotate, the detection frame drives the clamped sugarcane leaf or stalk to rotate, and the comprehensiveness of the sugarcane leaf or stalk during detection is guaranteed.

The invention relates to the technical field of genetic engineering, in particular to a gene MEU26 related to sexual coordination and pathopoiesis of sugarcane sporosmut, protein and application of the gene MEU26. The gene MEU26 is as shown in SEQ ID NO. 1. A delta MEU26 mutant strain is constructed through a PEG-mediated protoplast transformation method, a backfill strain is constructed through an ATMT method, it is found that the sexual cooperation ability and pathogenicity of the delta MEU26 mutant strain are affected, the sexual cooperation ability and pathogenicity of the backfill strain are basically recovered, and it is indicated that the MEU26 gene participates in regulation and control of the sexual cooperation and pathogenicity of the Saccharospora canadensis Saccharospora canadensis Saccharospora canadensis Saccharospora canadensis Saccharospora canadensis Saccharospora canadensis. A foundation is provided for screening and developing products for reducing pathogenicity of sugarcane sporoderm smut, and a new target is provided for researching and developing agents for preventing and treating sugarcane smut and a novel efficient disease prevention and control technology.

The invention discloses a compound microbial agent and application thereof in prevention and treatment of plant fungal diseases. The compound microbial agent is prepared from a Burkholderia ambifara strain Aa27 and a Bacillus velezensis strain Gc8, and the plant fungal disease is the sugarcane smut, and the plant fungal disease is the sugarcane smut. Experiments prove that both the Burkholderia ambifida strain Aa27 and the Bacillus velezensis strain Gc8 can inhibit the germination of teliospore and the sexual coordination of basidiospore of the sugarcane smut, and the composite microbial agent composed of the Burkholderia ambifida strain Aa27 and the Bacillus velezensis strain Gc8 not only can effectively prevent and treat the sugarcane smut, but also can be used for promoting the growth of the sugarcane and increasing the yield of the sugarcane. The compound microbial agent provided by the invention has important significance on prevention and treatment of fungal diseases of plants.

The present invention discloses a strain of Talaromyces stollii T-12, a microbial inoculum and its application, belonging to the technical field of biological control. This strain was deposited at the General Microbiological Center of the China Center for Type Culture Collection on December 9, 2024. The deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC No. 41659. The Talaromyces stollii T-12 disclosed by the present invention is a fungus isolated from soil and can colonize well in the soil. The present invention first discovers that this fungus has obvious inhibitory effects on Sporisorium scitamineum, Pseudomonas rubrilineans and Fusarium moniliforme var. subglutinans, and can be applied to the prevention and control of sugarcane smut, sugarcane brown stripe disease and sugarcane top rot disease. Especially, it has excellent control effect on sugarcane smut and has potential application value. At the same time, it also has certain growth-promoting potential and the ability to produce siderophores, and has broad prospects for popularization and application.

The present application relates to the technical field of sugarcane smut prevention and treatment, and particularly relates to a microbial fertilizer, a preparation method thereof and application thereof in prevention and treatment of smut. The microbial fertilizer comprises, by weight, 40-60 parts of cow dung, 2-4 parts of corn straw biochar, 4-6 parts of mulberry branch and 8-14 parts of mixed bacterial agent. The mixed bacterial agent is composed of Bacillus subtilis X1, Bacillus altitudinis X2, Bacillus cereus X3, Trichoderma harzianum Z1 and Trichoderma longibrachiatum Z2. The microbial fertilizer has a positive promoting effect on sugarcane and can significantly stimulate the self-defense response of sugarcane.

The invention discloses a pseudomonas bainite 2143-2 strain and an application of the pseudomonas bainite 2143-2 strain. The pseudomonas bainii 2143-2 strain disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center on September 2, 2025, and the preservation number is GDMCC No: 66909. The pseudomonas bainii 2143-2 strain disclosed by the invention has the advantages that the pseudomonas bainii 2143-2 strain is preserved in the Guangdong Microbial Culture Collection Center on September 2, 2025; the pseudomonas bainite 2143-2 strain not only can effectively inhibit the growth of sugarcane smut and remarkably reduce the morbidity of sugarcane smut, but also can effectively inhibit the growth of sugarcane leaf spot bacteria, sugarcane red rot bacteria, sugarcane brown streak bacteria, sugarcane ring spot bacteria and sugarcane tip rot bacteria, and the antibacterial rate reaches 65% or above. The pseudomonas bainite 2143-2 strain can be used for biological prevention and control of sugarcane smut, sugarcane leaf spot, sugarcane red rot, sugarcane brown streak, sugarcane ring spot and sugarcane tip rot, and development of sugarcane fungal disease prevention and control products is facilitated.

The invention discloses an application of a PRM1 gene in prevention and control of sugarcane smut, and the gene encodes a pheromone regulated multi-transmembrane protein which is a key factor for regulating sexual coordination and pathogenicity of pathogenic bacteria. Experiments show that inhibition or knockout of the PRM1 gene can significantly weaken the sexual cooperation ability of pathogenic bacteria, hinder the formation of binuclear hyphae and cause the reduction of the pathogenicity of the binuclear hyphae. In a molecular mechanism, PRM1 plays a role by influencing expression of a key gene of a pheromone signaling pathway. On the basis, the PRM1 gene and the encoding protein thereof are determined as a new target for preventing and treating the sugarcane smut, and can be used for developing novel green prevention and control technologies and products such as RNA interference preparations, specific inhibitors, gene editing breeding and engineering microorganisms, and a brand-new molecular basis and solution are provided for effectively controlling the sugarcane smut.

The invention discloses a SsTEM1 gene and application of the SsTEM1 gene in prevention and treatment of sugarcane smut, the nucleotide sequence of the SsTEM1 gene is shown as SEQ ID NO: 2, and a GTP binding protein containing an Spg1 structural domain is encoded. The key regulation effect of the SsTEM1 gene in the sexual coordination and pathopoiesis process of pathogenic bacteria is identified and confirmed for the first time: the SsTEM1 gene is highly expressed in a hypha stage; after the gene is knocked out, the cell wall integrity of the pathogenic bacteria is damaged, the sexual cooperation capability is obviously weakened, and the pathogenicity severely declines and even completely loses. Based on this, the present invention protects the gene and the protein sequence, and provides a control method using the gene as a target, including the control of diseases using a substance capable of inhibiting the expression of the gene or the activity of the protein, and a detection method and a disease-resistant plant cultivation method based on the gene. The invention provides a brand new molecular target and theoretical basis for developing an efficient and specific sugarcane smut green prevention and control technology.

The invention discloses a Burkholderia diphtheria 2143-6 strain and an application of the Burkholderia diphtheria 2143-6 strain. The Burkholderia diphtheria 2143-6 strain disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center on September 2, 2025, and the preservation number of the Burkholderia diphtheria 2143-6 strain is GDMCC No: 66910. The strain 2143-6 can inhibit the growth of sugarcane smut and reduce the morbidity of sugarcane smut, and can be used for preventing and treating sugarcane smut and reducing the adverse effect of sugarcane smut on the yield and the hammer sugar degree of sugarcane. Besides, the 2143-6 bacterial strain also has an inhibition effect on sugarcane leaf spot bacteria, sugarcane red rot bacteria, sugarcane brown streak bacteria and sugarcane ring spot bacteria, can be used for developing important products for preventing and treating sugarcane fungal diseases, and is beneficial to guaranteeing the yield and quality of sugarcanes.

The application provides application of prunol in prevention and treatment of sugarcane smut. It is verified through experiments that prunol can inhibit growth of haploid strains JG35 and JG36 of sugarcane smut fungus, and the inhibition on sexual compatibility of the two strains is more prominent, which provides a new way for green prevention and control of sugarcane smut. The method provided by the application has the advantages of green and no residue, simple operation, stable prevention and control effect and the like, and has a wide application prospect.

This application relates to a strain of *Pseudomonas lundense* and its applications. The present invention isolates a strain of *Pseudomonas lundense* from the root soil of rice plants in a rice cultivation base. This strain is effective against rice basal rot pathogens (…). Dickeya oryzae ), potato soft rot fungus ( Dickeya dadantii ), and Taro soft rot fungus ( Dickeya fangzhongdai It has a good control effect on sugarcane smut ( ) Sporisorium scitamineum ), rice blast fungus ( Magnaporthe oryzae ), Neodatura spp. Neoscytalidium dimidiatum ), neatly arranged small sclerotia ( Sclerotium rolfsii ), Lithocarpus ( Calonectria ), Black spores ( Nigrospora lacticoloni a) Ralstonia solanacearum ( Ralstonia solanacearum Xanthomonas aeruginosa (), Xanthomonas campestris pv. Campestris ) and citrus canker pathogen ( Xanthomonas citri subsp. citri It also has a good control effect. Therefore, it can be widely used in the control of plant diseases, including rice basal rot.

This invention relates to *Burkholderia gladioli* and its application in the preparation of products for controlling fungal diseases in sugarcane, belonging to the field of biological control technology. The *Burkholderia gladioli* of this invention is *Burkholderia gladioli*. Burkholderia gladioli Strain B1, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37420, is effective in controlling fungal diseases such as sugarcane smut, sugarcane top rot, sugarcane brown streak, and sugarcane red rot. It also possesses phosphorus-solubilizing and growth-promoting properties, thereby effectively promoting nutrient absorption and plant growth in crops.

This invention discloses a pathogenic factor of sugarcane smut and its application. This invention discovers... SsPHACA Application in regulating sexual mating, SDS tolerance, and pathogenicity of *Ustilago maydis*. This invention utilizes the principle of homologous recombination, employing a PEG-mediated protoplast transformation method to... SsPHACA Knockout and replacement were performed, resulting in knockout mutants and replacement strains. The results showed... SsPHACA Knockout resulted in a reduced ability of *Ustilago canescens* to form dimycelia through sexual mating, increased susceptibility to SDS, and decreased pathogenicity, indicating... SsPHACA This gene plays a crucial role in regulating the sexual mating, SDS tolerance, and pathogenicity of *Ustilago maydis*. This invention provides a novel target gene for the development of effective fungicides.

The invention discloses a method for improving the synergistic nitrogen fixation capacity of acid-aluminum-resistant sugarcane and microorganisms, and relates to the technical field of agricultural planting, and the method comprises the following steps: irrigating roots by using a nitrogen-fixing bacterial agent in the tillering stage of the sugarcane; the nitrogen-fixing bacterial agent is prepared from the following raw material components: 2 to 3 parts of pseudomonas stutzeri, 3 to 5 parts of brown nitrogen-fixing bacteria, 1 to 2 parts of pantoea agglomerans and 0.1 to 2 parts of maleic anhydride. By optimizing the composition of the nitrogen-fixing bacteria, the synergistic nitrogen-fixing effect of the sugarcane and microorganisms is promoted, the combination of the nitrogen-fixing bacteria can perform metabolic activity in soil and sugarcane seed stems, saccharides, amino acids, vitamins, nitrogen elements, bioactive substances and the like generated by metabolism can be absorbed by the sugarcane seed stems, growth of roots of the sugarcane seed stems can be promoted, and the growth rate of the sugarcane seed stems is increased. The acid-aluminum resistance of the sugarcane is improved; pseudomonas stutzeri and azotobacter brown produce acid to chelate aluminum in soil, the content of active aluminum in the soil is reduced, and pantoea agglomerans are cooperated to effectively prevent and treat sugarcane smut.

The invention relates to the technical field of biology, in particular to a bactericidal composition and application thereof. The bactericidal composition is prepared from the following raw materials in parts by weight: 20 to 30 parts of mancozeb, 40 to 50 parts of carbendazim and 10 to 20 parts of a microbial preparation. The bactericidal composition has a very wide application prospect in biological prevention and control of sugarcane smut.

The invention discloses a method for simultaneously evaluating smut resistance and brown streak resistance of sugarcane, and belongs to the field of sugarcane disease resistance evaluation. The method comprises the following steps: (1) collecting teliospores of sugarcane smut and culturing sugarcane brown streak bacteria; (2) artificially inoculating sugarcane smut by adopting a seed soaking method, and inoculating sugarcane brown streak after the sugarcane smut is inoculated for 30-35 days and sugarcane seedlings grow to 25-35 cm; (3) evaluating the disease resistance of the sugarcane smut: when the sugarcane seedlings grow out and the first smut occurs, starting to investigate the seedling emergence plant number and the morbidity plant number of the sugarcane, and dividing the disease resistance grade according to the morbidity; evaluating the disease resistance of the sugarcane brown streak: investigating the morbidity of the sugarcane brown streak 7-10 days after inoculating the sugarcane brown streak, and dividing disease resistance grades according to the area of infected leaves. According to the method, the disease resistance evaluation results of the two diseases can be obtained at the same time through one-time testing, and technical support is provided for prevention and treatment of smut and brown streak disease of sugarcane, rapid screening of disease-resistant breeding and breeding of disease-resistant varieties.

The invention belongs to the technical field of biological prevention and control of plant diseases, and particularly relates to a biological source rhizosphere metabolite phthalic acid and application thereof in prevention and control of sugarcane smut. The invention relates to an application of a biological source rhizosphere metabolite in prevention and control of sugarcane smut. The biological source rhizosphere metabolite is phthalic acid. The key rhizosphere metabolite phthalic acid with efficient and targeted bacteriostatic activity is screened and identified from disease-inhibiting soil, safe and efficient prevention and control of sugarcane smut are achieved by targeted inhibition of germination of pathogenic bacteria chlamydospore, and the key rhizosphere metabolite phthalic acid can be developed as a biological source pesticide and has broad application prospects. And the method has great significance on pesticide reduction, synergism and green development of agriculture.