11 results about "Sugarcane smut" patented technology

The invention provides a sustained-release and controlled-release sugarcane bacteriostatic pesticide-fertilizer particle and a preparation method and application thereof, and belongs to the technical field of agricultural sustained-release and controlled-release fertilizer.The pesticide-fertilizer particle is coated with chitosan-sodium alginate double-network hydrogel, has the pH intelligent response characteristic and can adjust the nutrient and bactericide release rate in a self-adaptive mode according to the soil pH change; oxathiapiprolin and pyraclostrobin are compounded, so that sugarcane smut, maculopathy and rust disease are prevented and treated in a broad-spectrum and high-efficiency manner; a biomass charcoal-bentonite-zeolite molecular sieve composite carrier and a bacillus amyloliquefaciens composite flora are introduced, so that the micro-ecology of the soil is improved; a three-layer coating structure simplifies the preparation process, the production cost is reduced, and field tests show that the pesticide-fertilizer particles provided by the invention can increase the yield of sugarcane by 12-18%, increase the sugar content by 0.5-1.2%, reduce the disease incidence by 70-80%, increase the nutrient utilization rate by 20-35%, and increase the soil organic matter content by 0.6-4.6 g / kg.

The invention relates to the technical field of sugarcane smut resistance, and provides a molecular marking method for sugarcane smut resistance, which comprises a sample preparation unit, a double-sugarcane area inoculation identification unit, a whole genome association analysis unit, an SNP (Single Nucleotide Polymorphism) marker development unit, a marker verification unit and a result application unit, the output end of the sample preparation unit is connected with the input end of the double-sugarcane-area inoculation identification unit through a sample transmission channel, the double-sugarcane-area inoculation identification unit outputs phenotypic data to the whole-genome association analysis unit, and the whole-genome association analysis unit outputs remarkably associated SNP sites to the SNP marker development unit; and the SNP marker development unit outputs a specific primer to the marker verification unit. The molecular marker provided by the invention is based on the correlation between the candidate gene of the site and the known disease-resistant pathway of the plant, and the MYB transcription factor can improve or reduce the resistance level of the plant to pathogenic bacteria by regulating the synthesis of secondary metabolites, transduction of plant hormone signals, expression of defensive genes and the like.

The invention relates to the technical field of genetic engineering, in particular to a gene MEU26 related to sexual coordination and pathopoiesis of sugarcane sporosmut, protein and application of the gene MEU26. The gene MEU26 is as shown in SEQ ID NO. 1. A delta MEU26 mutant strain is constructed through a PEG-mediated protoplast transformation method, a backfill strain is constructed through an ATMT method, it is found that the sexual cooperation ability and pathogenicity of the delta MEU26 mutant strain are affected, the sexual cooperation ability and pathogenicity of the backfill strain are basically recovered, and it is indicated that the MEU26 gene participates in regulation and control of the sexual cooperation and pathogenicity of the Saccharospora canadensis Saccharospora canadensis Saccharospora canadensis Saccharospora canadensis Saccharospora canadensis Saccharospora canadensis. A foundation is provided for screening and developing products for reducing pathogenicity of sugarcane sporoderm smut, and a new target is provided for researching and developing agents for preventing and treating sugarcane smut and a novel efficient disease prevention and control technology.

The invention discloses an application of a PRM1 gene in prevention and control of sugarcane smut, and the gene encodes a pheromone regulated multi-transmembrane protein which is a key factor for regulating sexual coordination and pathogenicity of pathogenic bacteria. Experiments show that inhibition or knockout of the PRM1 gene can significantly weaken the sexual cooperation ability of pathogenic bacteria, hinder the formation of binuclear hyphae and cause the reduction of the pathogenicity of the binuclear hyphae. In a molecular mechanism, PRM1 plays a role by influencing expression of a key gene of a pheromone signaling pathway. On the basis, the PRM1 gene and the encoding protein thereof are determined as a new target for preventing and treating the sugarcane smut, and can be used for developing novel green prevention and control technologies and products such as RNA interference preparations, specific inhibitors, gene editing breeding and engineering microorganisms, and a brand-new molecular basis and solution are provided for effectively controlling the sugarcane smut.

The invention discloses a SsTEM1 gene and application of the SsTEM1 gene in prevention and treatment of sugarcane smut, the nucleotide sequence of the SsTEM1 gene is shown as SEQ ID NO: 2, and a GTP binding protein containing an Spg1 structural domain is encoded. The key regulation effect of the SsTEM1 gene in the sexual coordination and pathopoiesis process of pathogenic bacteria is identified and confirmed for the first time: the SsTEM1 gene is highly expressed in a hypha stage; after the gene is knocked out, the cell wall integrity of the pathogenic bacteria is damaged, the sexual cooperation capability is obviously weakened, and the pathogenicity severely declines and even completely loses. Based on this, the present invention protects the gene and the protein sequence, and provides a control method using the gene as a target, including the control of diseases using a substance capable of inhibiting the expression of the gene or the activity of the protein, and a detection method and a disease-resistant plant cultivation method based on the gene. The invention provides a brand new molecular target and theoretical basis for developing an efficient and specific sugarcane smut green prevention and control technology.

The application provides application of prunol in prevention and treatment of sugarcane smut. It is verified through experiments that prunol can inhibit growth of haploid strains JG35 and JG36 of sugarcane smut fungus, and the inhibition on sexual compatibility of the two strains is more prominent, which provides a new way for green prevention and control of sugarcane smut. The method provided by the application has the advantages of green and no residue, simple operation, stable prevention and control effect and the like, and has a wide application prospect.

This application relates to a strain of *Pseudomonas lundense* and its applications. The present invention isolates a strain of *Pseudomonas lundense* from the root soil of rice plants in a rice cultivation base. This strain is effective against rice basal rot pathogens (…). Dickeya oryzae ), potato soft rot fungus ( Dickeya dadantii ), and Taro soft rot fungus ( Dickeya fangzhongdai It has a good control effect on sugarcane smut ( ) Sporisorium scitamineum ), rice blast fungus ( Magnaporthe oryzae ), Neodatura spp. Neoscytalidium dimidiatum ), neatly arranged small sclerotia ( Sclerotium rolfsii ), Lithocarpus ( Calonectria ), Black spores ( Nigrospora lacticoloni a) Ralstonia solanacearum ( Ralstonia solanacearum Xanthomonas aeruginosa (), Xanthomonas campestris pv. Campestris ) and citrus canker pathogen ( Xanthomonas citri subsp. citri It also has a good control effect. Therefore, it can be widely used in the control of plant diseases, including rice basal rot.

This invention relates to *Burkholderia gladioli* and its application in the preparation of products for controlling fungal diseases in sugarcane, belonging to the field of biological control technology. The *Burkholderia gladioli* of this invention is *Burkholderia gladioli*. Burkholderia gladioli Strain B1, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37420, is effective in controlling fungal diseases such as sugarcane smut, sugarcane top rot, sugarcane brown streak, and sugarcane red rot. It also possesses phosphorus-solubilizing and growth-promoting properties, thereby effectively promoting nutrient absorption and plant growth in crops.

This invention discloses a pathogenic factor of sugarcane smut and its application. This invention discovers... SsPHACA Application in regulating sexual mating, SDS tolerance, and pathogenicity of *Ustilago maydis*. This invention utilizes the principle of homologous recombination, employing a PEG-mediated protoplast transformation method to... SsPHACA Knockout and replacement were performed, resulting in knockout mutants and replacement strains. The results showed... SsPHACA Knockout resulted in a reduced ability of *Ustilago canescens* to form dimycelia through sexual mating, increased susceptibility to SDS, and decreased pathogenicity, indicating... SsPHACA This gene plays a crucial role in regulating the sexual mating, SDS tolerance, and pathogenicity of *Ustilago maydis*. This invention provides a novel target gene for the development of effective fungicides.

The invention discloses a multi-mode sugarcane smut detection platform based on a space separation strategy and application of the multi-mode sugarcane smut detection platform, and relates to the technical field of biosensing and chemical analysis. The invention provides a spatially separated multi-mode sensing platform aiming at the problems that traditional single signal detection is easily interfered to cause false positive or false negative, and common multi-mode detection may cause signal crosstalk and the like. The platform is creatively integrated with three functional modules which are separated in space, namely a folded double-chain DNA modified magnetic bead module, a Y-type three-chain DNA modified ITO electrode module and a CRISPR / Cas12a functionalized DNA hydrogel module, wherein the three functional modules are the folded double-chain DNA modified magnetic bead module, the Y-type three-chain DNA modified ITO electrode module and the CRISPR / Cas12a functionalized DNA hydrogel module. After a target gene is subjected to specific recognition and amplification, independent output of three signals of photoelectrochemistry, electrochemistry and fluorescence is realized through cooperation of the three signals. According to the method, false positive is effectively inhibited through three-mode signal cross validation, crosstalk between detection modes is eliminated in combination with a space separation strategy, and the efficient and accurate multi-mode detection method for early diagnosis and field monitoring of sugarcane smut is provided.

The invention discloses application of an RWTD1 gene in prevention and control of sugarcane smut. The RWTD1 gene is a key factor for regulating sexual coordination of pathogenic bacteria and development of pathogenic symptoms (black whips). Through functional verification, pathogenic bacteria cannot complete the whole life history after RWTD1 gene deletion: even if the pathogenic bacteria can infect hosts, plants cannot be induced to form black whips and generate teliospore, so that a disease transmission chain is cut off. On the basis, the RWTD1 gene, the encoding protein thereof or a related regulation and control sequence are used as prevention and control targets, various application ways including RNA interference, gene editing, chemical inhibition and disease-resistant breeding are provided, and a core molecular basis and a brand new solution are provided for developing a new efficient, accurate and environment-friendly sugarcane smut prevention and control technology.