20results about How to "Promote colonization" patented technology

The present application relates to the technical field of skin external agent, in particular to a kind of regulating sensitive skin oil function composition and application thereof, the composition is composed of chrysopridine extract, fructo-oligosaccharide, carrier;The chrysopridine extract 0.005%-0.5wt%, fructo-oligosaccharide 0.02%-7wt%, the rest is carrier.This application explores the efficacy of chrysopridine extract, and finds that chrysopridine extract can promote the colonization of beneficial bacteria in microecological regulation, and by matching fructo-oligosaccharide, not only significantly improve the colonization number of beneficial bacteria, but also reduce the adhesion of harmful bacteria, with the function of bidirectional regulation of microecology;In addition, the present application can play different skin care effects by matching chrysopridine extract and fructo-oligosaccharide for different positions of face, has obvious oil control effect in T zone prone to secrete oil, the oil control effect is weak in U zone prone to dry, but has good moisturizing effect.The present application can provide convenient and fast care products for consumers with mixed sensitive skin.

The present invention provides a high-protein bio-fermented aquatic feed and a preparation method thereof. The aquatic feed comprises the following raw materials in parts by weight: 20-30 parts of imported fish meal, 10-15 parts of compound minerals, 5-10 parts of bioactive peptides, 5-10 parts of lysine, 5-10 parts of compound trace elements, 3-8 parts of compound vitamins, 3-6 parts of herbal extracts, 2-5 parts of compound probiotics, 2-4 parts of immune polysaccharides, and 1-3 parts of compound enzymes; the herbal extracts are prepared by fermenting astragalus membranaceus, isatis root, liquorice root, calotropis gigantea, ventilago leiocarpa benth, clove, rhizoma chuanxiong, and galangal officinale. By organically integrating imported fish meal, astaxanthin-peptide chelate, herbal extracts, compound immune polysaccharides, compound probiotics and various nutrients, the present invention realizes the synergistic effect of "promoting feeding - enhancing digestion - strengthening liver and gall - enhancing immunity - resisting stress - inhibiting bacteria - stabilizing water quality", significantly improves the growth rate, survival rate and comprehensive breeding benefit of crustacean aquatic animals, and has good economic value and application prospect.

This invention belongs to the field of traditional Chinese medicine fermentation technology, specifically involving a high-enzyme-active Liu Shen Qu (a type of fermented medicinal herb) and its fermentation process. The process first processes red beans, bitter almonds, artemisia annua, cocklebur, and polygonum hydropiper into a medicinal porridge, which is then mixed with wheat flour and a specific fermenting agent to form a soft mass. Its innovation lies in the use of a Bacillus subtilis trehalose protective agent complex and Aspergillus oryzae chitosan microspheres. These two fermenting agents are prepared by spray drying encapsulation and ionogel methods, respectively, significantly improving the survival rate and stability of the fermenting microorganisms during processing and storage. The soft mass undergoes two stages of precisely controlled temperature and humidity fermentation, and finally, after shaping and segmented drying, the finished product is obtained. This process effectively solves the problems of low enzyme activity and poor stability in traditional Liu Shen Qu fermentation, resulting in a Liu Shen Qu product with high digestive enzyme activity and stable, uniform quality.

The present application relates to a kind of water body ecological restoration method, comprising the following steps: S1, constructs the array of enclosure in water body, each enclosure unit in array is closed enclosure;Array in the same row of enclosure unit between there is spacing, there is spacing between the same column of enclosure unit;S2, each enclosure unit is planted submersed plant, and the enclosure unit that cultivation is obtained in line with preset vegetation coverage;S3, at least two adjacent enclosure units in line with preset vegetation coverage are connected and merged, form recovery module, again submersed plant is planted to recovery module, to make recovery module in line with preset vegetation coverage;S4, recovery module in line with preset vegetation coverage is connected with the water body outside module, forms recovery area.Its beneficial effect is, beneficial to underwater vegetation rapid recovery, effectively establish underwater ecosystem, maintain water ecological balance and self-purification capacity, to improve water quality.

This invention provides a method for controlling Spartina alterniflora and promoting natural mangrove restoration based on rolling mulching and in-situ mud slurry, belonging to the field of ecological control technology. The method involves mechanically rolling Spartina alterniflora to flatten most of it and press it into the tidal flat soil. Then, a biodegradable mulch film is laid on top, and mud slurry is extracted and applied to form a mud slurry cover layer. A mangrove seed interception and planting device is then installed on the mud slurry cover layer to prevent mangrove seeds from growing naturally, thereby reducing the cost of Spartina alterniflora control and improving the efficiency of Spartina alterniflora control.

The invention discloses a composite microbial agent containing pseudomonas and application thereof, and belongs to the technical field of agricultural microorganisms. The composite microbial agent disclosed by the invention comprises pseudomonas synthetic flora and N-acetyl-L-tryptophan, wherein the N-acetyl-L-tryptophan can be used for effectively inhibiting the growth of pathogenic bacteria and effectively improving the colonization capability of pseudomonas in soil; the pseudomonas and the N-acetyl-L-tryptophan are combined for use, so that the relative abundance of beneficial bacteria in continuous cropping soil can be improved, and the relative abundance of harmful bacteria can be reduced; the positive correlation proportion of continuous cropping soil microorganism interaction is increased; the apple rhizosphere microenvironment is remodeled, and the growth of apple seedlings in the continuous cropping soil is promoted, so that the prevention and treatment effect on the apple continuous cropping obstacles is remarkably improved.

The invention discloses a biochar-polyurethane carbon anode material, a preparation method thereof, a microbial fuel cell and application, biochar, polyol, triethanolamine, water and isocyanate are added into a mold to be mixed to obtain a mixture, the mixture reacts under stirring until white floating foam appears, then standing foaming is performed, carbonization is performed after the material is cured, and the biochar-polyurethane carbon anode material is obtained. The internal resistance of a double-chamber microbial fuel cell constructed by taking the carbonized biochar-polyurethane material as an anode of MFCs is not greater than 3.00 omega, the highest voltage stably exceeds 620 mV, the maximum area power density exceeds 800 mW m <-2 >, and the COD removal rate of the biochar-polyurethane carbon anode material disclosed by the invention reaches 97.24%.

The application discloses a pollen-derived flavone-containing plant stress resistance inducer and a preparation method thereof. The application applies a strain-inducer matching directional induction fermentation strategy to the preparation of pollen flavone: according to the selected type of fungi, yeast or lactic acid bacteria, a cellulose disaccharide, salicin or myricetin is matched and added as an induction substrate to specifically activate the overexpression of a beta-glucosidase system, so that low-activity flavone glycosides are efficiently converted into high-activity flavone aglycone, and the conversion rate reaches 50%-80%; metabolic products generated by fermentation form an in-situ synergistic system with the flavone aglycone. The application is also first used as a plant stress resistance inducer. The application has simple process and high conversion rate, and can significantly improve the resistance of plants to low temperature, drought and other stress.

The invention belongs to the field of agricultural biotechnology and organic fertilizers, and discloses a compound microbial agent enhanced organic liquid fertilizer and a preparation method thereof.The organic liquid fertilizer is composed of a compound functional microbial agent, a difunctional liquid carrier system and an intelligent activation factor, wherein the composite functional microbial agent comprises nitrogen-fixing bacteria, phosphate solubilizing bacteria and bacillus, and nutrient conversion and rhizosphere micro-ecology construction are promoted through a synergistic metabolism relationship; the difunctional liquid carrier system provides nutrition for crops and forms a liquid phase environment suitable for long-term stable survival of microorganisms; the intelligent activation factor is used for triggering microorganisms to be converted from a low-metabolism silent state to an activated state under the induction of crop rhizosphere secretions and environmental signals, so that stable storage before application and accurate activation release after application are realized. The survival rate and the utilization efficiency of thalli in the liquid microbial fertilizer are effectively improved, the application effect of the fertilizer is improved, and the liquid microbial fertilizer has a good application prospect.

The utility model provides a bacterial liquid spraying device for manufacturing a mouse skin bacterial infection model, which comprises a fixed cylinder and two support rods, a through groove is arranged at the top of the fixed cylinder, a mounting groove is arranged at the front end of the through groove at the top of the fixed cylinder, a fixing mechanism is arranged in the fixed cylinder, sliding grooves are arranged at the top of the fixed cylinder on both sides of the through groove, and the sliding grooves are communicated with the through groove. Mounting plates are fixedly connected to the two ends of the top of the fixed cylinder, a motor is fixedly mounted on one side of the mounting plate at one end, a moving mechanism is arranged between the two mounting plates, and limiting mechanisms are arranged on the portions, on the two sides of the moving mechanism, between the two mounting plates. And then a moving block is driven to move, so that the moving block drives an adjusting plate below to move from the tail of the nude mouse to the head of the nude mouse, and uniform spraying is performed on the skin of the nude mouse through a through groove by utilizing a bacterial liquid spray head, so that the accuracy and repeatability of a research result are better, and the biological safety is better guaranteed.

This invention discloses a strain of *Boletus urinaria* PP013 and its applications, with the preservation number CGMCC No. 40661. This invention also provides a sustainable, cyclical understory cultivation method, including liquid spawn culture, spawn culture, soil covering for fruiting, and understory inoculation. This method selects shallow-rooted tree species such as *Delonix regia* and *Gardenia jasminoides*, and lays a layer of dry weeds consisting of sugarcane bagasse, miscanthus, and *Digitaria sanguinalis* under the trees. Artificially cultivated fruiting bodies about to open their gills are placed under the trees as a live spore source for spore production. After spore production, the understory is covered with dry weeds and watered. The following year, after fruiting, some fruiting bodies are retained for natural spore production, achieving a single inoculation and multi-year cyclical fruiting. This invention creatively utilizes fruiting body spores for natural inoculation, replacing the traditional direct mycelial inoculation method. It boasts a high success rate of establishment, low long-term production costs, and represents a highly efficient forest-fungus integrated management model.

This invention belongs to the field of feed additive technology, specifically relating to a microalgae compound feed additive for alleviating weaning stress in calves and its preparation method. The microalgae compound feed additive comprises the following raw materials in parts by weight: 5-10 parts microalgae powder, 2-6 parts unsaturated fatty acid / modified montmorillonite complex, 0.7-1.2 parts probiotics, and 0.3-0.5 parts walnut shell extract. This microalgae compound feed additive can improve the fragile intestinal barrier function and immunity of weaned calves, enhance their growth performance, and thus improve breeding efficiency.

The application discloses a method for preparing ecological restoration soil by repairing contaminated spoil through regeneration, and comprises the following steps: mixing dry straw powder and bimetallic alkali liquor, stirring and drying, pyrolyzing under a protective atmosphere at a temperature above 500 DEG C to obtain modified biochar material; mixing the modified biochar material with microbial liquid, oscillating and adsorbing, and freeze-drying to obtain carbon-based biological composite material; mixing the carbon-based biological composite material with shield spoil to obtain improved shield spoil; and mixing the improved shield spoil with organic fertilizer to obtain ecological restoration soil; the method removes toxic and harmful components in the shield spoil through biodegradation, the modified biochar improves the structure of the shield spoil, and the specific component compound fertilizer is used, so that the shield spoil is recycled, and the shield spoil-based ecological restoration soil is obtained.

The application discloses a double-layer microcapsule containing lactobacillus plantarum and a preparation method and application thereof, and application of lactobacillus plantarum EASY 0352 in preparation of anxiolytic and depressive products. 2+ The metal-phenol network coating is formed on the surface of the bacteria by coordination self-assembly, the single-cell coating microcapsule can significantly enhance the adhesion of lactobacillus plantarum EASY 0352 to intestinal epithelial cells, and is beneficial to the colonization of lactobacillus plantarum EASY 0352 in the intestinal tract; on the basis of the single-cell coating microcapsule, the application further coats transglutaminase (TG) and whey protein concentrate (WPC) composite wall materials to form a double-layer microcapsule; and simulation of gastric and intestinal fluid digestion experiment results show that the double-layer microcapsule prepared by the application can significantly improve the survival rate of lactobacillus plantarum EASY 0352 in simulated gastric and intestinal fluid, and effectively protects the probiotics to pass through the gastrointestinal tract smoothly.

The invention relates to the technical field of organic fertilizers, and discloses an RNAi medical fertilizer granule for preventing and treating rice borer and a preparation method thereof, the RNAi medical fertilizer granule comprises a core-shell-coat three-layer composite structure; the core layer is composed of a basic fertilizer, an inhibitor, a nutrient loss control material and a water retention synergist; the shell layer is a core-shell particle formed by uniformly spraying a nano-composite medicine-carrying liquid formed by a cationic polymer, target dsRNA and an ultraviolet protective agent on the surface of the core layer; the coating layer is formed by uniformly spraying an inducing layer coating solution prepared from an attractant and a degradable adhesive on the surface of the shell layer to form core-shell-coating particles. Through a core-shell-coat three-layer composite structure design, a nano-composite-physical embedding-environment isolation multi-protection system is constructed, conversion from passive pesticide application to active trapping and killing is realized, the delivery efficiency of dsRNA is improved, and the problem that dsRNA stably exists and efficiently enters pest bodies is effectively solved.

This invention belongs to the field of environmental engineering technology, and specifically discloses a method for treating phytoremediation-derived biomass containing heavy metals. This method utilizes bone meal to immobilize heavy metals in the biomass through a co-hydrothermal process, controlling the leaching of Cd within a safe threshold range and Zn far below the safe threshold. This ensures that the resulting hydrothermal carbon composite material does not pose an ecological risk due to excessive heavy metal release. Furthermore, the hydrothermal carbon composite material obtained by this method possesses characteristics such as uniformity, porosity, and a large specific surface area, exhibiting excellent heavy metal adsorption capacity, thus enabling its reuse as a soil remediation agent. This method eliminates the need for separating heavy metals from the phytoremediation-derived biomass, is simple in process, and avoids problems such as acid washing wastewater pollution and treatment.

This invention relates to the field of biopharmaceutical technology, and in particular to an antibody-targeted modified dual-strain prebiotic delivery system, its preparation method, and its applications. This invention modifies VCAM-1 antibody fragments onto *AKK* bacteria and *Bifidobacterium lactis* Probio-M8, then utilizes fructooligosaccharides and inulin as prebiotics to enhance probiotic metabolism and promote intestinal colonization. Finally, it coats the system with hydroxypropyl methylcellulose phthalate (HPMCP) enteric coating to construct the antibody-targeted modified dual-strain prebiotic delivery system—V-PM8-Am-FI-HPMC. The V-PM8-Am-FI-HPMC provided by this invention can simultaneously achieve intestinal flora regulation, lipid metabolism balance, inflammation suppression, ischemia improvement, and vascular anti-calcification, ultimately achieving the purpose of treating and / or improving atherosclerosis, while ensuring targeting and safety.

This invention discloses a compound microbial agent containing Pseudomonas and its application, belonging to the field of agricultural microbiology technology. The compound microbial agent of this invention includes a Pseudomonas synthetic flora and N-acetyl-L-tryptophan. N-acetyl-L-tryptophan can effectively inhibit the growth of pathogens and effectively improve the colonization ability of Pseudomonas in the soil. The combined use of Pseudomonas and N-acetyl-L-tryptophan can increase the relative abundance of beneficial bacteria and decrease the relative abundance of harmful bacteria in continuously cropped soils; increase the positive correlation ratio of microbial interactions in continuously cropped soils; reshape the rhizosphere microenvironment of apples; and promote the growth of apple seedlings in continuously cropped soils, thereby significantly improving the control effect on continuous cropping obstacles in apples.

This invention discloses a method for regulating banana resistance to Fusarium oxysporum using aminooxyacetic acid (AOA), belonging to the field of plant protection technology. This invention reveals the mechanism by which Foc4 pathogen infection harms bananas. Foc4 infection causes a large accumulation of reactive oxygen species (ROS), exacerbating oxidative stress, inducing cell death and vascular tissue necrosis, ultimately leading to Fusarium wilt symptoms. Exogenous application of aminooxyacetic acid (AOA) can effectively inhibit excessive ROS accumulation, enhance the antioxidant system's defense capabilities, reduce cell damage, maintain vascular structure integrity, and significantly alleviate disease symptoms. Based on this regulatory mechanism, intervening in the oxidative stress pathway can effectively control Fusarium wilt in bananas, showing promising application prospects.

The present application relates to a kind of dental implants for repairing alveolar bone defect, the dental implant includes abutment, implant and side wing, three are integrally connected;The lower part of abutment is connected with the upper part of implant and is provided with side wing, the side wing is the integrated composite structure formed by reinforcing layer and porous layer.Two side wings of the dental implant are astride on alveolar bone surface, two side wings effectively disperse stress, and two side wings produce good clamping effect to alveolar bone, avoid making dental implant sink, rotate and loosen in bone alveolar bone, provide better initial stability.And the porous layer of side wing plays the role of bone graft, the shape and size of porous layer can be designed according to the shape and size of alveolar bone bone defect part.The implant of the dental implant and the porous layer of side wing have multi-scale aperture porous structure, promote bone tissue to grow into porous structure, so that implant and alveolar bone form bone integration, realize long-term biological fixation.