67results about How to "Promote colonization" patented technology

The invention provides a strain of Burkholderia graminicola as well as a bacterial agent and application of the Burkholderia graminicola. The preservation number of the Burkholderia graminicola is CCTCC (China Center For Type Culture Collection) NO: M20242399. The Burkholderia paragallinarum provided by the invention has the functions of nitrogen fixation, phosphate solubilization, siderophore production and indoleacetic acid production. The Burkholderia grassy and the fungicide taking the Burkholderia grassy as the effective active component can promote the germination of plant seeds and the growth of crops.

The invention discloses a lactobacillus delbrueckii subsp. Lactis TG001 and application thereof in improving oral health, and belongs to the technical field of probiotic application, the preservation number of the lactobacillus delbrueckii subsp. Lactis TG001 is CCTCC NO: M 20241941, the preservation place is China Center for Type Culture Collection in Wuhan, and the preservation date is September 9, 2024. The lactobacillus delbrueckii subsp. Lactis TG001 has a good inhibition effect on common oral pathogens such as streptococcus mutans and porphyromonas gingivalis, particularly can inhibit the growth of cariogenic bacteria streptococcus mutans by generating hydrogen peroxide and remove biofilm formation, and has good tolerance to antibacterial substances in an oral environment. Therefore, the oral cavity diseases such as dental caries, periodontitis and ozostomia can be prevented, the micro-ecological environment of the oral cavity is effectively improved, meanwhile, the peculiar smell of the oral cavity is relieved, and good application prospects are achieved in preparation of products for preventing and / or treating the oral cavity diseases such as dental caries and periodontitis.

The invention discloses a livestock composite biological feed prepared from probiotics and a method thereof, and relates to the technical field of livestock feeds. The compound biological feed for the livestock and poultry is prepared from the following raw materials: lactobacillus plantarum BNCC138404, bacillus coagulans BNCC132591, bacillus velezensis BNCC352076, bifidobacterium bifidum BNCC186493, aspergillus niger BNCC147472, enterococcus faecalis BNCC186075, yarrowia lipolytica BNCC193899, nutrient substances, mineral trace elements, vitamins, amino acids and prebiotics. The compound biological feed for the livestock and poultry is prepared from the following raw materials: lactobacillus plantarum BNCC138404, bacillus coagulans BNCC132591, bacillus velezensis BNCC352076, bifidobacterium bifidum BNCC186493, aspergillus The compound biological feed fermented by adopting various probiotics can improve the antioxidant level of the broiler chicken, improve the health condition of intestinal tracts, increase the villus height and the villus-implicit ratio of the jejunum of the broiler chicken and reduce the occurrence of intestinal inflammation, so that the growth performance of the broiler chicken is promoted, and the immune performance of the body is improved.

The invention provides a plant lactobacillus for promoting skeletal development in a growth period as well as application and a product of the plant lactobacillus, and belongs to the technical field of microorganisms. The invention provides a lactobacillus plantarum BL25 and a probiotic preparation thereof, the preservation number of the lactobacillus plantarum BL25 is CGMCC (China General Microbiological Culture Collection Center) No.33117, stachyose is adopted as prebiotics, and compared with other prebiotics, the lactobacillus plantarum BL25 can obviously promote the activity of the lactobacillus plantarum BL25. The plant lactobacillus BL25 and the probiotic preparation thereof have the effect of resisting the severe environment of gastrointestinal tracts, and can increase the length of femur and bone density, promote the development of a bone microstructure, improve bone metabolism, increase osteogenesis markers and reduce osteoclast markers, so that the skeletal development in the life growth period is promoted.

The invention provides a fungus for antagonizing sexual cooperation of smut smut of sugarcane and application thereof, and belongs to the technical field of biological prevention and control. The blue vibrio T-15 provided by the invention is a fungus separated from soil and can be better colonized in the soil, and it is found for the first time that the fungus can effectively antagonize sugarcane smut fungi for sexual cooperation to enable the sugarcane smut fungi to lose pathogenicity, can be applied to prevention and treatment of sugarcane smut, and has potential application value; the blue virens T-15 has an inhibition effect on the infection of the sugarcane smut fungi; the blue virens T-15 also has certain growth promoting potential and has the capability of producing siderophores; the invention provides a new fungus resource for biological control of sugarcane smut.

The invention belongs to the technical field of novel biological building materials, and particularly relates to a method for reinforcing sandy soil by utilizing cement hydration and biological cement bonding, which comprises the following steps: inoculating urease bacteria into a culture medium for culturing to obtain a bacteria solution; preparing a cementing liquid matched with the urease bacteria; portland cement and sand are evenly stirred and filled into a mold, and a sand column blank is obtained; and spraying the bacterial liquid and the cementing liquid from the top of the sand column blank in turn to carry out a microbial mineralization reaction so as to obtain the molded sand column. According to the invention, the hydration effect of Portland cement is cooperated with biological cement to fully fill gaps of sand grains, so that a high-strength composite cementing system is formed. According to the method, the compressive strength of the sandy soil is remarkably improved, particularly, the defect that the strength of the sandy soil subjected to cementation treatment through a single biological grouting method is insufficient can be overcome, powerful guarantee is provided for safety and stability in the fields of foundation reinforcement, structural defect repair and the like, and remarkable academic innovation and engineering application value are achieved.

The invention discloses a rice arbuscular mycorrhiza specifically induced nuclear factor YC subunit gene and application thereof. The nucleotide sequence of the rice nuclear factor YC subunit gene OsNF-YC3 is as shown in SEQ ID NO. 1. The amino acid sequence of the protein OsNF-YC3 coded by the gene OsNF-YC3 is as shown in SEQ ID NO. 2. The rice nitrate transporter gene OsNF-YC3 is applied to promotion of interaction of plants and arbuscular mycorrhizal fungi and improvement of phosphorus absorption in the symbiotic process of the plants.

The invention discloses an iron-aluminum modified carbon-based coated organic-inorganic slow-release microbial compound fertilizer and a preparation method thereof. The iron-aluminum modified carbon-based coated organic-inorganic slow-release microbial compound fertilizer comprises a compound fertilizer master batch and a tobacco straw carbon coating layer wrapping the compound fertilizer master batch, bacteria are planted in the tobacco straw carbon coating; through mutual cooperation of the compound fertilizer master batch, the tobacco stalk carbon coating and the bacteria, the effects of improving the utilization rate of fertilizer nutrients and reducing the application amount of the fertilizer are achieved, on one hand, the tobacco stalk carbon coating can improve the water-holding capacity of soil, and the porosity of the soil can be improved due to the characteristics of high porosity and high specific surface area of the tobacco stalk carbon coating; on the one hand, the tobacco stalk charcoal coating layer is used as a coating layer, the slow release effect is achieved, coordinated and lasting release of nutrient elements is facilitated, the utilization efficiency of fertilizer nutrients is high, and repeated topdressing is avoided, and on the other hand, the tobacco stalk charcoal coating layer is used as a field planting carrier, so that the effect of improving the soil structure and the acid soil is achieved. And colonization of bacteria is facilitated.

The invention belongs to the technical field of soil improvement, and particularly relates to a green moss charcoal-microorganism complex for improving saline-alkali soil and improving saline-alkali tolerance of plants as well as a preparation method and application of the green moss charcoal-microorganism complex. The nitrogen-fixing bacteria have a nitrogen-fixing effect, the preristeria megatherium has a potassium-dissolving effect, the nitrogen-fixing bacteria and the preristeria megatherium are combined with the green moss-derived biochar, and the green moss biochar is small in aperture, can effectively adsorb metal cations in saline-alkali soil, has strong affinity to the nitrogen-fixing bacteria and the preristeria megatherium, and can effectively adsorb the metal cations in saline-alkali soil. The nitrogen-fixing bacteria and the P.megatherium can be effectively adsorbed, so that the colonization of the nitrogen-fixing bacteria and the P.megatherium on the surface of a plant root system and in rhizosphere soil is enhanced; meanwhile, the nitrogen-fixing bacteria and the preristeria megatherium attached to the biochar can relieve saline-alkali stress of the rhizosphere microenvironment, the resistance of crops to the saline-alkali environment is improved, the growth of the crops is promoted, and a new thought is provided for stress resistance and yield increase of the crops in the saline-alkali environment.

The present application relates to the technical field of skin external agent, in particular to a kind of regulating sensitive skin oil function composition and application thereof, the composition is composed of chrysopridine extract, fructo-oligosaccharide, carrier;The chrysopridine extract 0.005%-0.5wt%, fructo-oligosaccharide 0.02%-7wt%, the rest is carrier.This application explores the efficacy of chrysopridine extract, and finds that chrysopridine extract can promote the colonization of beneficial bacteria in microecological regulation, and by matching fructo-oligosaccharide, not only significantly improve the colonization number of beneficial bacteria, but also reduce the adhesion of harmful bacteria, with the function of bidirectional regulation of microecology;In addition, the present application can play different skin care effects by matching chrysopridine extract and fructo-oligosaccharide for different positions of face, has obvious oil control effect in T zone prone to secrete oil, the oil control effect is weak in U zone prone to dry, but has good moisturizing effect.The present application can provide convenient and fast care products for consumers with mixed sensitive skin.

The present invention provides a high-protein bio-fermented aquatic feed and a preparation method thereof. The aquatic feed comprises the following raw materials in parts by weight: 20-30 parts of imported fish meal, 10-15 parts of compound minerals, 5-10 parts of bioactive peptides, 5-10 parts of lysine, 5-10 parts of compound trace elements, 3-8 parts of compound vitamins, 3-6 parts of herbal extracts, 2-5 parts of compound probiotics, 2-4 parts of immune polysaccharides, and 1-3 parts of compound enzymes; the herbal extracts are prepared by fermenting astragalus membranaceus, isatis root, liquorice root, calotropis gigantea, ventilago leiocarpa benth, clove, rhizoma chuanxiong, and galangal officinale. By organically integrating imported fish meal, astaxanthin-peptide chelate, herbal extracts, compound immune polysaccharides, compound probiotics and various nutrients, the present invention realizes the synergistic effect of "promoting feeding - enhancing digestion - strengthening liver and gall - enhancing immunity - resisting stress - inhibiting bacteria - stabilizing water quality", significantly improves the growth rate, survival rate and comprehensive breeding benefit of crustacean aquatic animals, and has good economic value and application prospect.

The invention provides lactobacillus plantarum NX69 and application thereof, the lactobacillus plantarum NX69 is lactobacillus plantarum NX69, the lactobacillus plantarum NX69 is preserved in the Guangdong Microbial Culture Collection Center on November 21, 2024, the address is No.59 building, No.100 courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC No.65517; the lactobacillus plantarum (Lactobacillus plantarum) NX69 is used for preventing and treating the abalone vibriosis, and the lactobacillus plantarum (Lactobacillus plantarum) NX69 can be used for preventing and treating the abalone vibriosis. The strain disclosed by the invention has relatively strong inhibition capability on abalone vibriosis, relatively strong tolerance on acid, good cholate resistance, good automatic agglutination capability and hydrophobicity, and is beneficial to colonization at parts such as intestinal mucosa and the like.

This invention belongs to the field of traditional Chinese medicine fermentation technology, specifically involving a high-enzyme-active Liu Shen Qu (a type of fermented medicinal herb) and its fermentation process. The process first processes red beans, bitter almonds, artemisia annua, cocklebur, and polygonum hydropiper into a medicinal porridge, which is then mixed with wheat flour and a specific fermenting agent to form a soft mass. Its innovation lies in the use of a Bacillus subtilis trehalose protective agent complex and Aspergillus oryzae chitosan microspheres. These two fermenting agents are prepared by spray drying encapsulation and ionogel methods, respectively, significantly improving the survival rate and stability of the fermenting microorganisms during processing and storage. The soft mass undergoes two stages of precisely controlled temperature and humidity fermentation, and finally, after shaping and segmented drying, the finished product is obtained. This process effectively solves the problems of low enzyme activity and poor stability in traditional Liu Shen Qu fermentation, resulting in a Liu Shen Qu product with high digestive enzyme activity and stable, uniform quality.

The invention provides a Lactobacillus casei DS31 microcapsule for improving irritable bowel syndrome and a preparation method thereof, the Lactobacillus casei DS31 microcapsule loaded with DS31 is prepared by using the Lactobacillus casei DS31 as a water phase, DHA algal oil as an oil phase, thermally denatured whey protein as solid particles and chitosan oligosaccharide as a stabilizer by adopting a Pickering emulsion technology, and the microcapsule loaded with DS31 is prepared by using the Lactobacillus casei DS31 as a water phase, DHA algal oil as an oil phase and chitosan oligosaccharide as a stabilizer. The obtained microcapsule can resist the severe environment of gastrointestinal tracts and help strains to be planted in the intestinal tracts better, and the strain effect of the strains is achieved. The emulsion in the microcapsule has the advantages of small droplet size, high surface area, good physical stability, simplicity in operation, safety, no toxicity, high probiotic content and the like.

The invention discloses a chondroitin sulfate / whey protein isolate anthocyanin nano-composite and a preparation method and application thereof.The preparation method comprises the steps that blueberry pomace is pre-frozen, freeze-dried and then ground into powder, blueberry pomace powder is obtained, the blueberry pomace powder and pectinase are added into a citric acid buffer solution, water bath oscillation enzymolysis is conducted, the blueberry pomace powder and pectinase are taken out for enzyme deactivation, and the chondroitin sulfate / whey protein isolate anthocyanin nano-composite is obtained. The preparation method comprises the following steps: dissolving whey protein isolate and chondroitin sulfate into water, carrying out ultrasonic treatment, centrifuging, filtering supernate, purifying and freeze-drying to obtain anthocyanin powder, dissolving whey protein isolate and chondroitin sulfate into water, regulating the pH value, dropwise adding an anthocyanin solution into a chondroitin sulfate solution, carrying out secondary stirring, dropwise adding the whey protein isolate solution, and carrying out three-time stirring, standing, centrifuging, freeze-drying and grinding to obtain the product. According to the method, the extraction rate of the anthocyanin is high, the embedding rate of the nanocomposite is high, the stability is good, and the method is green, efficient and simple.

The invention belongs to the field of coatings, and particularly relates to a microbial coating and a preparation method thereof, the microbial coating comprises a porous silicon-based material containing microorganisms, a curing agent containing calcium ions and a thickening agent; the curing agent containing calcium ions comprises urea, water-soluble calcium salt and water, the microorganisms are microorganisms for producing carbonic anhydrase; and mixing the porous silicon-based material, a thickening agent and a curing agent to obtain the microbial coating. The microbial coating can capture carbon dioxide in air and induce calcium ions in the environment to deposit and separate out calcium carbonate, and can be used for defect repair of walls.

The present application relates to a kind of water body ecological restoration method, comprising the following steps: S1, constructs the array of enclosure in water body, each enclosure unit in array is closed enclosure;Array in the same row of enclosure unit between there is spacing, there is spacing between the same column of enclosure unit;S2, each enclosure unit is planted submersed plant, and the enclosure unit that cultivation is obtained in line with preset vegetation coverage;S3, at least two adjacent enclosure units in line with preset vegetation coverage are connected and merged, form recovery module, again submersed plant is planted to recovery module, to make recovery module in line with preset vegetation coverage;S4, recovery module in line with preset vegetation coverage is connected with the water body outside module, forms recovery area.Its beneficial effect is, beneficial to underwater vegetation rapid recovery, effectively establish underwater ecosystem, maintain water ecological balance and self-purification capacity, to improve water quality.

The invention belongs to the technical field of biology, and discloses a pediococcus acidilactici strain capable of relieving constipation and diarrhea, the name of the pediococcus acidilactici strain is P48, the classification name of the pediococcus acidilactici strain is pediococcus acidilactici, the preservation number of the pediococcus acidilactici strain is CGMCC No.27885, the preservation date of the pediococcus acidilactici strain is July 13, 2023, and the preservation unit of the pediococcus acidilactici strain is China General Microbiological Culture Collection Center, No. 3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing. The pediococcus acidilactici P48 can tolerate the gastrointestinal tract environment, improves the survival rate of the gastrointestinal tract, is beneficial to strain colonization, and can be used for preparing functional microbial agents, food and medicines for relieving constipation or diarrhea; the pediococcus acidilactici P48 disclosed by the invention can be used for carrying out bidirectional regulation on diarrhea and constipation at the same time; meanwhile, intestinal flora imbalance can be improved, immune system activation is promoted, inflammatory factor release is inhibited, and the gastrointestinal tract function is improved.

This invention provides a method for controlling Spartina alterniflora and promoting natural mangrove restoration based on rolling mulching and in-situ mud slurry, belonging to the field of ecological control technology. The method involves mechanically rolling Spartina alterniflora to flatten most of it and press it into the tidal flat soil. Then, a biodegradable mulch film is laid on top, and mud slurry is extracted and applied to form a mud slurry cover layer. A mangrove seed interception and planting device is then installed on the mud slurry cover layer to prevent mangrove seeds from growing naturally, thereby reducing the cost of Spartina alterniflora control and improving the efficiency of Spartina alterniflora control.

The utility model discloses a kind of sponge city efficient permeation greenbelt planting structures, it includes: green grass layer, artificial backfill nutrient soil layer, non-woven geotextile, water-accumulating layer and original soil layer, water-accumulating layer, non-woven geotextile, artificial backfill nutrient soil layer and green grass layer are sequentially laid from bottom to top in the upper of original soil layer, first drainage pipeline is provided in original soil layer, a plurality of rootlet pipes are provided on first drainage pipeline, a plurality of rootlet pipes are arranged along the direction of first drainage pipeline, one end of rootlet pipe extends into water-accumulating layer, a plurality of first through holes are provided on rootlet pipe, overflow pipe is provided on first drainage pipeline, overflow pipe extends to green grass layer upwards, overflow hole is provided on first drainage pipeline, overflow hole bottom is connected with second drainage pipeline, second drainage pipeline is connected with water-collecting well, and sealing assembly is provided at overflow hole. The utility model can solve the problem of poor permeability of urban green space compared with prior art.

The invention discloses a microfiller for culturing sewage treatment aerobic granular sludge and a preparation method of the microfiller, belongs to the technical field of sewage treatment, and relates to the microfiller for culturing the sewage treatment aerobic granular sludge. The invention aims to solve the problems of long granulation time and poor stability in the prior art. The microfiller for culturing the aerobic granular sludge for sewage treatment is prepared by uniformly mixing one or more of straw powder, beneficial metal salt and red mud with plastic particles, heating, melting and granulating to obtain fine particles rich in organic matters, and aging the fine particles rich in organic matters by ultraviolet light. According to the microfiller disclosed by the invention, an internal core of the aerobic granular sludge is formed in a form of rapid colonization of microorganisms, and a novel aerobic granular sludge system with a stable core is formed through induction, so that the bottleneck problems of long granulation time (2-5 months) and poor stability existing in an aerobic granular sludge technology at present are solved.

The invention relates to the technical field of culture, in particular to a microecological preparation and a method for applying the microecological preparation to industrial culture of penaeus vannamei boone, and the method comprises the following steps: step S1, separation, identification and safety analysis of intestinal tract or healthy culture water strains of penaeus vannamei boone; and S2, influence of the strain on the growth performance of the penaeus vannamei. According to the method, strains are screened and separated from intestinal tracts and culture water bodies of the penaeus vannamei, the safety of the strains is analyzed, the influence of the screened strains on the growth performance of the penaeus vannamei is evaluated by applying different types and numbers of strains and planting modes in the culture process, and the microecological preparation is developed by optimizing a thallus formula and a production process; the method is applied to industrial aquaculture of the penaeus vannamei and realizes green and healthy aquaculture of the penaeus vannamei.

The present invention discloses a potato soft rot antagonistic Bacillus subtilis 20Q2B‑18‑1 and its application, and relates to the field of biotechnology. The Bacillus subtilis 20Q2B‑18‑1 was deposited in the Guangdong Provincial Microbial Culture Collection Center on September 26, 2022, with a preservation address of 5th Floor, Building 59, No. 100, Xianlie Middle Road, Guangzhou, and a preservation number of GDMCC No: 62836. The Bacillus subtilis 20Q2B‑18‑1 provided by the present invention can be well colonized on Qingshu No. 9 and Qingshu No. 2, and it has an inhibitory effect on the soft rot pathogens of potato in both treatment and protection modes, and can induce SOD (superoxide dismutase), CAT (catalase), PPO (polyphenol oxidase) and POD (peroxidase) enzyme activities to increase.

The invention discloses a composite microbial agent containing pseudomonas and application thereof, and belongs to the technical field of agricultural microorganisms. The composite microbial agent disclosed by the invention comprises pseudomonas synthetic flora and N-acetyl-L-tryptophan, wherein the N-acetyl-L-tryptophan can be used for effectively inhibiting the growth of pathogenic bacteria and effectively improving the colonization capability of pseudomonas in soil; the pseudomonas and the N-acetyl-L-tryptophan are combined for use, so that the relative abundance of beneficial bacteria in continuous cropping soil can be improved, and the relative abundance of harmful bacteria can be reduced; the positive correlation proportion of continuous cropping soil microorganism interaction is increased; the apple rhizosphere microenvironment is remodeled, and the growth of apple seedlings in the continuous cropping soil is promoted, so that the prevention and treatment effect on the apple continuous cropping obstacles is remarkably improved.

The invention discloses a biochar-polyurethane carbon anode material, a preparation method thereof, a microbial fuel cell and application, biochar, polyol, triethanolamine, water and isocyanate are added into a mold to be mixed to obtain a mixture, the mixture reacts under stirring until white floating foam appears, then standing foaming is performed, carbonization is performed after the material is cured, and the biochar-polyurethane carbon anode material is obtained. The internal resistance of a double-chamber microbial fuel cell constructed by taking the carbonized biochar-polyurethane material as an anode of MFCs is not greater than 3.00 omega, the highest voltage stably exceeds 620 mV, the maximum area power density exceeds 800 mW m <-2 >, and the COD removal rate of the biochar-polyurethane carbon anode material disclosed by the invention reaches 97.24%.

This invention relates to the field of microbial technology, disclosing a strain of *Streptomyces oryzae*, its inoculant, and its applications. The *Streptomyces oryzae* strain provided by this invention exhibits excellent abilities to degrade benzoic acid, solubilize organophosphates, secrete auxin (IAA), and possess broad-spectrum inhibition against various pathogens. Application of this strain and related preparations to the plant rhizosphere can effectively improve soil quality, control soil-borne diseases, and promote plant growth. Furthermore, since this bacterium originates from the Wuliang Mountain Nature Reserve in Yunnan Province and is a natural rhizosphere fungus, it possesses good colonization capabilities. Moreover, as it originates from virgin forest soil, it has a greater ability to adapt to complex environments than general strains, thus enabling it to control the occurrence and spread of soil-borne diseases and promote plant growth.

The invention discloses an L-malic acid and bifidobacterium breve composition and application thereof in preparation of a product for preventing colitis, and belongs to the technical field of enteric microorganisms. According to the invention, the L-malic acid and the bifidobacterium breve are combined for use, so that the colitis symptom can be more effectively improved. L-malic acid promotes field planting and reproduction of bifidobacterium breve in intestinal tracts by enhancing the viability of bifidobacterium breve in the intestinal tracts, so that the probiotic effect of bifidobacterium breve is better played, and colitis is improved.

The invention discloses a probiotic freeze-dried active bacterium bacteriostatic tablet for gynecology. The probiotic freeze-dried active bacterium bacteriostatic tablet is specifically prepared from the following components in parts by weight: active bacteria of probiotics, a freeze-drying protective agent, soybean peptide powder, montmorillonite, D-mannitol, fructo-oligosaccharide, sorbitol, microcrystalline cellulose, chlorhexidine acetate and magnesium stearate. The invention further discloses a preparation method of the gynecological probiotic freeze-dried active bacterium bacteriostatic tablet. According to the invention, the survival rate and the survival time of probiotics are effectively prolonged, and the purpose of micro-ecological balance is improved, so that the effect of preventing original bacteria from breeding is achieved.

The invention discloses an application of intestinal symbiotic bacteria in preparation of products for preventing and treating colorectal cancer. The intestinal symbiotic bacteria are ruminococcus flavus or succinic acid producing filamentous bacteria. The method has the beneficial effects that the intestinal flora balance can be regulated; the intestinal barrier function can be enhanced; the immunoregulation effect is achieved; the effect of comprehensively preventing and treating colorectal cancer is achieved.

The present application provides Bacillus siamensis, Bacillus velezensis, Serratia marcescens, and microbial agents and pesticides, and applications thereof. The present application has significant effects on early warning and prevention and control of pine wood nematode disease.