395results about How to "Promote colonization" patented technology

A bicomposite material based on collagen is prepared which has two closely bound layers and is biocompatible, non-toxic, hemostatic and biodegradable in less than a month, and can be used in surgery to achieve hemostasis and prevent post-surgical adhesion. To prepare the material, a solution of collagen or gelatin, which may contain glycerine and a hydrophilic additive such as polyethylene glycol or a polysaccharide, is poured onto an inert support to form a layer 30 .mu.m to less than 100 .mu.m thick. Then a polymeric porous fibrous layer is applied during gelling of the collagen or gelatin, and the resultant material is dried. The polymeric porous fibrous layer may be made of collagen or a polysaccharide, and have a density of not more than 75 mg / cm.sup.2, a pore size from 30 .mu.m to 300 .mu.m and a thickness of 0.2 cm to 1.5 cm.

Disclosed are nutritional compositions including human milk oligosaccharides that can be administered to individuals including preterm infants, infants, toddlers, and children for improving gastrointestinal function and tolerance, as well as the growth of beneficial bacteria. Additional suitable methods of using the nutritional compositions including the human milk oligosaccharides are also disclosed.

A bicomposite material based on collagen is prepared which has two closely bound layers and is biocompatible, non-toxic, hemostatic and biodegradable in less than a month, and can be used in surgery to achieve hemostasis and prevent post-surgical adhesion. To prepare the material, a solution of collagen or gelatin, which may contain glycerine and a hydrophilic additive such as polyethylene glycol or a polysaccharide, is poured onto an inert support to form a layer 30 μm to less than 100 μm thick. Then a polymeric porous fibrous layer is applied during gelling of the collagen or gelatin, and the resultant material is dried. The polymeric porous fibrous layer may be made of collagen or a polysaccharide, and have a density of not more than 75 mg / cm2, a pore size from 30 μm to 300 μm and a thickness of 0.2 cm to 1.5 cm.

The invention provides a lactobacillus helveticus microcapsule as well as the preparation and application thereof, pertaining to the embedding technology of lactobacillus helveticus and aiming at microencapsulating the lactobacillus helveticus so as to solve the problems of low embedding efficiency and embedding yield in single-layer or double-layer embedment. The technical proposal adopted by the invention is that isolated soy protein, microporous starch and sodium alginate are respectively taken as the first, the second and the third layers of wall materials for microencapsulation; the three-layer embedment is carried out to the lactobacillus helveticus for the first time, wherein, in the process of preparing bacterial suspension, an orthogonal optimization protective agent is adopted to improve the livability after freeze drying. The microcapsule of the invention has strong heat resistance and acid resistance, solves the inactivation problem caused by gastric acid, digestive enzyme and antibiotic, etc. to active bacillus, is capable of releasing when reaching large intestine, and balances intestinal flora. The reusability of fermented milk is good. The lactobacillus helveticus used by the invention enriches the variety of probiotic bacteria microcapsule and is a good dietary supplement.

The invention relates to a method for associating plants and microorganisms to accumulate heavy metal cadmium in soil. The method comprises the following steps: firstly planting cadmium accumulated plants in cadmium polluted soil; then inoculating cadmium-resistant bacteria in rhizosphere soil of the cadmium accumulated plants; conventionally culturing, watering and moisturizing; enriching cadmium in cadmium accumulated soil by utilizing the absorption effect of the cadmium accumulated plants to the cadmium and reinforcing effect of the cadmium-resistant bacteria; repeating the process so that the purpose of repairing the cadmium polluted soil can be achieved. The soil repairing method is good in effect, low in cost, easy to manage and operate, and free of secondary pollution, and has a wide application prospect.

The invention discloses a preparation method of an embedding microbial agent for in-situ purification of a water body, which compounds a plurality of microbial strains suitable for the sewage treatment with enzyme preparation, and adds nutritive salt and growth factors needed by the microbial growth in an embedding carrier so as to be more suitable for the microbial growth, so that the defects of deficient nutrition of the water body and low sewage treatment efficiency are overcome. Active carbons with strong adsorption capacity and diatomite with greater specific gravity are added in the embedding carrier; polyglutamic acid is added in the embedding carrier; the polyglutamic acid reinforces the strength of embedding particles; meanwhile, glutamic acid obtained by degrading the polyglutamic acid can be used as nutritional ingredients for the strain growth, so that preparation can be preferably colonized in a river channel without easily losing. After the embedding microbial agent is used for treating sewage for three weeks, the ammonium-nitrogen removal rate reaches 85-90%, and the COD (Chemical Oxygen Demand) removal rate reaches 90-95%.

The present invention discloses a watermelon juice probiotic fermented beverage and a preparation method thereof. The beverage preparation method uses watermelons as raw materials, scientifically blends prebiotic factors, does not add any essences and coloring matters, mainly uses low-temperature processing technology such as ultrasonic wave cleaning, high-pressure pulsed electric field treatment, biological enzymolysis, vacuum degassing, etc. in the whole process, replaces the existing heat sterilization and enzyme blunting technology, maintains the color and luster, flavor and mouth feel of the natural watermelon juice to a maximum degree, enhances the non-biological stability and biological stability of the watermelon juice, reduces the addition of chemical additives, increases the juice squeezing rate and food safety of the watermelon juice, and has a probiotic live bacteria content of 7 multiplied by 10^11 to 8 multiplied by 10^11 cfu / ml. The watermelon juice probiotic fermented beverage is environmentally friendly, can be processed into probiotic solid beverage and quantitatively packaged beverage which have natural taste, are nutritious and health-care, are convenient to drink, and have longer shelf lives, opens up a new way for watermelon deep processing and industrial development, and can achieve better economic and social benefits.

An improved filamentous fungi is disclosed that has an enhanced nutritional profile by utilizing pulsed ultraviolet irradiation. According to the invention, the vitamin D component of mushrooms and other filamentous fungi may be drastically increased with no deleterious affects on appearance with the use of pulsed UV radiation. Mushrooms so treated had up to 1800% DV in one serving of fresh mushrooms.

The invention relates to a stent vessel for interventional therapy and a dedicated traction tool for implanting the stent vessel into a human body. The stent vessel comprises an artificial or animal vessel and support rings, wherein the support rings in three structures are used for fixation and respectively arranged at the outlet end and the inlet end of the artificial or animal vessel, and in the support rings, a detachable support ring with radial resilience force is further provided. The dedicated traction tool is especially suitable for dragging a Y-shaped stent into circuitous or narrow parts of the human body. The stent vessel provided by the invention is extremely flexible and easy to be implanted into a branch vessel until to the circuitous part. Because the main part and the branch of the stent vessel are integrated, the stent vessel is easy to position and safe and reliable without leakage after being implanted into the human body; and as the structure is simple and the cost is low, stent vessels in various diameters, variable diameters and lengths are be made, and the application range is wide because of no limit to lengths.

The present invention relates to a preparation method of a lactic acid bacterium microcapsule. The preparation method comprises the following steps: firstly, lactic acid bacteria and prebiotics are used to obtain a synbiotic solution; secondly, sodium alginate and pectin are dissolved in distilled water and stirred at room temperature to obtain a thick mixed solution, then CaCO<3> powder is uniformly dispersed in the mixed solution to obtain a microcapsule wall material, and the microcapsule wall material and the synbiotic solution are evenly mixed to obtain a mixed bacteria and pectin solution; and thirdly, the microcapsule is prepared. The prepared lactic acid bacterium microcapsule can tolerate effects of stomach acid and bile salt and thus better colonize intestines, also exerts probiotic effects of many lactic acid bacteria in regulating immunity, improving digestion, preventing tumors and mutation, adjusting acid-base balance, at the same time is even in particles, and has a particle size of about 100 [mu]m.

The invention discloses plant growth promoting bio-organic fertilizer for saline-alkali land, and belongs to the technology of soil improvement. The bio-organic fertilizer produced by fermenting agricultural organic solid wastes through microbial strains can increase the survival rate and promote growth of salt-tolerant plants planted in mud-flat soil and increases a soil fertility improvement speed and speeds up an improvement progress. Compost is subjected to secondary fermentation by trichoderma asperellum 12 and bacillus subtilis D9, wherein the content of trichoderma asperellum 12 in a product is larger than or equal to 0.2*10<8> cfu (colony forming unit) g<-1>, the content of bacillus subtilis D9 in the product is larger than or equal to 0.5*10<8> cuf g<-1>, a pH value is 5.5, the mass ratio of the water content is smaller than or equal to 30% and the mass ratio of organic matter content is larger than or equal to 45%. An experiment indicates that compared with treatment without application of the bio-organic fertilizer, the biomass of salt-tolerant plants including suaeda galuce, cyperus malaccensis and kochia scoparia planted in soil is increased by 49.1%, 56.3% and 52.2 respectively after the bio-organic fertilizer is applied to the mud-flat saline-alkali soil.

The invention relates to a preparation method of a porcine vascular cell-free stent with the combination of chemistry and physics, which mainly includes the steps of material preparation, membrane rupture of superficial cells, removal of superficial cells, removal of deep cells and so on. Surfactant is used for the membrane rupture of deep cells at the same time of completing the removal task of superficial cells, so the incubation and oscillation only need to be carried out for about 1 hour in the surfactant solution with appropriate concentration, and the removal task of the deep cells can be completed by the ultrasonic waves with lower power in a shorter time; the mutual cooperation can allow the porcine vascular cell-free stent to have the advantages of lower equipment cost, shorter preparation time, thorough removal of the cells, easy clean of the surfactant, complete preservation of extracellular matrix (stent), good biocompatibility, appropriate degree of biodegradation rate, high mechanical strength, easy suture and fixation and so on; furthermore, the stent is also conductive to the colonization and growth of human cells and can be gradually degraded and absorbed along with the regeneration of real blood vessel.

The invention relates to a biological control agent, a bioorganic fertilizer, and applications thereof. The biological control agent comprises Bacillus velezensis, Bacillus subtilis, Paenibacillus polymyxa, and Bacillus amyloliquefaciens. The provided biological control agent and bioorganic fertilizer can continuously inhibit fusarium oxysporum for a long term, and has a long-lasting control effect on plant blight, in particular, banana blight.

The invention provides a new technological method for preparing and industrially culturing aweto strains. The method comprises the following steps of: using young silkworms of 2 years as a host of an aweto culture medium, preparing strains by separating the tissue of wild awetos, dying the young silkworms, providing biological conditions suitable for aweto growth, and culturing aweto products with the shapes of wild awetos and the similar contents of various food mass. The invention has simple and convenient operation process, high yield rate and low culture cost.

The invention provides Bacillus velezensis K01 and application thereof. The Bacillus velezensis K01 has the growth promotion functions of dissolving phosphorus and generating indoleacetic acid, cellulase, protease and the like, also can generate anti-bacteria active substances and volatile anti-bacteria substances, can inhibit growth of various pathogenic bacteria, has a good biocontrol function, also can favorably realize colonization on the rhizosphere of a plant and in the plant, can be applied to plant growth promotion and biological control of plant diseases, and has a good application prospect.

A method for assisting in tailings restoration through ryegrass and planting the ryegrass on restored land includes the following steps that tailings is turned over by 18-22 cm and leveled; fresh ryegrass growing to the florescence are shorn into broken segments being 3-5 cm long so that ryegrass green manure can be formed; the ryegrass green manure is spread on the tailings land which is turned over and leveled, and the ryegrass green manure and the tailings are fully mixed evenly so that smooth planting land can be formed; ryegrass seeds are sown on the planting land. The planting method includes the steps that the seeds are processed and sown; the ryegrass is harvested regularly while stubble is left and the harvested ryegrass is returned to the field, 3-5 years after the ryegrass is planted, the whole ryegrass plants are recycled, the land is turned over, then, ridges are reconstructed for planting, and the plating method is repeated in this way. Due to the virtuous cycle that the planted ryegrass is returned to the field, the ecological environment can be continuously improved, and the landscape phenomenon of the tailings can be improved. The method has the advantages that operability is high and ecologically friendly and restoration cost is substantially reduced and the method is an important measure for ecological restoration of the tailings.

The invention provides a composite bacillus preparation containing three strains, a preparation method of the composite bacillus preparation and application of the composite bacillus preparation to ecological breeding, and belongs to the technical field of microorganism and feed additives. Bacillus coagulans, butyric acid clostridia and bacillus megaterium are used as fermentative strains respectively, and large-scale, low-cost and high-density fermentation production of spores is conducted through step-by-step magnification; three live bacterium preparations are prepared by mixing bacterial sludge with the spores and dry starch; the three live bacterium preparations are matched according to the equal weight ratio, the composite bacillus preparation is obtained, and the concentration of the spores reaches more than 1*108-109 cfu / g; the composite bacillus preparation serves as a microorganism and feed additive to be added to farmed animal feed according to the mass ratio ranging from 0.02% to 0.5%. The composite bacillus preparation is simple and stable in process, low in cost, high in spore content and environment tolerance, easy to store and high in survival rate; the purposes of composition and synergism are achieved through the collaborative supplementary effects of different bacilli in the aspect of micro-ecology adjustment, growth of farmed animals is promoted, the feed utilization rate and feed rewards are increased, diarrhea is prevented and reduced, and the breeding ecological environment is improved.

The present invention discloses lactobacillus rhamnosus, named lactobacillus rhamnosus HCS01-003. The strain is preserved on August 14, 2018 in the "China General Microbiological Culture Collection Center", and the preservation number is CGMCC No.16257. The invention also discloses an application of the strain used as an additive lactobacillus rhamnosus lyophilized powder which has strong anti-digestive tract anti-environment ability and good bacteriostatic property in preparing foods, health foods or pharmaceutical compositions. The invention further discloses an application of the strain used as a food additive lactobacillus rhamnosus lyophilized powder which has strong anti-digestive tract anti-environment ability and good bacteriostatic property in preparing infant foods.

The invention discloses a probiotic liquid preparation based on a double-emulsion structure and a preparation method thereof. The method comprises the following steps that S1, a probiotic bacterium suspension, a thickener and an intestinal tract auxiliary release material (namely a material capable of assisting in the release of probiotics in an intestinal tract) are mixed, and induction is carried out with bivalent salt ions to form a gel-like inner water phase W1; S2, an oil-soluble emulsifier is dissolved in edible oil to form an oil phase O, and the oil phase O and the inner water phase W1are subjected to high-speed shearing and mixing so as to obtain a water-in-oil type primary emulsion W1 / O; and S3, a suspension of colloidal particles is served as an outer water phase W2, the outerwater phase W2 is added into the primary emulsion W1 / O, and intermediate-speed shearing and mixing are carried out so as to obtain the probiotic emulsion preparation based on the W1 / O / W2 type double-emulsion structure. According to the probiotic liquid preparation and the preparation method thereof, protective embedding is carried out on probiotics by adopting a Pickering double-emulsion system, so that the probiotic effect is fully guaranteed, meanwhile, the probiotics can be prevented from being damaged by oxygen and the environment of a gastrointestinal tract, and therefore the action of gastric acid and cholate can be resisted, in addition, colonization can be better carried out in the intestinal tract, and a wide application prospect is achieved.

The invention discloses feed for weaned piglets. The feed comprises the following raw materials in parts by weight: 45-55 parts of corn, 10-20 parts of wheat, 5-10 parts of extruded soybean, 10-20 parts of bean pulp, 3-7 parts of wheat middling, 1-4 parts of soybean oil, 1-4 parts of steamed fish powder, 2-5 parts of glucose, 0.2-0.4 part of acidifying agent, 1.2-1.5 parts of calcium hydrophosphate, 0.7-1.0 part of stone powder, 0.1-0.3 part of salt, 0.3-0.5 part of compound amino acid, 0.1-0.3 part of compound vitamin, 0.1-0.3 part of compound trace element, 0.05-0.15 part of enzyme preparation and 0.05-0.15 part of microecological preparation. The feed is used for the weaned piglets with the age of 6-10 weeks, and has the beneficial effects that the feed intake of the weaned piglets can be increased, the growth can be enhanced, the feed reward can be improved, the diarrhea rate and the feed-changing stress of the weaned piglets can be reduced and the use of antibiotics and bad results caused by the antibiotics are reduced.

The invention relates to the technical field of a biological agent, and particularly relates to a cellulose degradation bacteria agent and a raw material strain, and preparation methods and applications of the cellulose degradation bacteria agent and raw material strain. The compound bacteria agent for degrading cellulose mainly comprises a paecilomyces varioti MM2 strain, an aspergillus fumigates MM6 strain, a candida rugosa MJ4 strain, and a bacillus licheniformis MX8 strain. The cellulose can be efficiently degraded and utilized by the compound bacteria agent disclosed by the invention, the compound bacteria agent can be used as a detonating agent and an enhancer of wine sediment compost, the compost process can be accelerated, the compost rotten degree is improved, an organic fertilizer is fabricated, and the cellulose degradation bacteria agent is especially applicable to resourceful treatment of lignocelluloses solid waste and production of the organic fertilizer by using the wine sediment.

The invention discloses antagonistic bacteria for prevention and treatment of tobacco bacterial wilt and application thereof. The antagonistic bacteria are Bacillus cereus QJ-1 and Aspergillus niger Ty-3. Specifically, the preservation unit of QJ-1 is China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, China, the preservation date is July 5, 2012, and the preservation number is: CCTCC, NO:M2012271; and the preservation unit of Ty-3 is China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, China, the preservation date is July 8, 2011, and the preservation number is CCTCC NO:M2011241. The antagonistic bacteria, i.e. the Bacillus cereus QJ-1 and Aspergillus niger Ty-3 provided in the invention can be combined into a microbial agent. The microbial agent and an organic material can be fermented into a microbial organic fertilizer, which can provide effective nutrition and functional bacteria, and realize effective prevention and treatment of tobacco bacterial wilt.

The invention relates to a weight reducing and fat reducing special type clinic nutrition recipe, which comprises 20 to 38 parts of protein, 8 to 12 parts of fat, 40 to 50 parts of carbohydrate, 5 to 8 parts of dietary fiber, 0.3 to 1.5 parts of macro elements, 0.01 to 0.04 part of trace elements, 0.005 to 0.015 part of lipid-soluble vitamin, 0.01 to 0.3 part of water-soluble vitamin, 0.05 to 1 part of dietary essence, 0.1 to 2 parts of medical and edible ingredients, 0.1 to 3 parts of natural plant compounds and 0.005 to 2 parts of new resource food, wherein the medical and edible ingredients are selected from at least one of poria cocos, fructus lycii, lotus leaves, semen cassiae, radix platycodi, rhizoma dioscoreae, fructus crataegi and coix seeds; each ingredient is prepared into powder in advance; the fat and the vitamin are prepared into powder by using a micro-capsule embedding technology. Through the screening and the proportioning of the ingredients, the nutrition recipe provides reasonable and overall nutrition support for patients with adiposity; meanwhile, the medical and edible ingredients, the natural plant compounds and the new resource food with the effects of replenishing qi to invigorate the spleen, eliminating phlegm, removing dampness and reducing weight and fat are added, so that the effects of preventing and treating the adiposity are achieved.

The invention relates to a probiotic solid beverage and a preparation method thereof. The probiotic solid beverage is prepared from, by weight, 25-30 parts of inulin, 5-10 parts of cranberry extractive and 1-5 parts of probiotics; the probiotics comprises bifidobacterium lactis, lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus casei, lactobacillus reuteri, lactobacillus plantarumand bifidobacterium longum. The probiotic solid beverage can reduce the loss of the probiotics in the process of passing through the gastrointestinal tract, improve the survival rate, promote the colonization of the probiotics in the intestinal tract, has the functions of adjusting intestines and stomach, relieving constipation and diarrhea, adjusting body immunity and adjusting the blood glucoselevel, particularly can significantly improve gynecologic inflammation and female blotches, dark skin and dry hair caused by the constipation.

The present invention includes a contaminant-degrading composition for use in remediation of contaminated soil having a selected contaminant. Such a composition can include a seed for a plant capable of growing in the presence of the selected contaminant, and a contaminant-degrading mycobacteria on the seed. Additionally, the present invention includes a contaminant-degrading system for use in remediation of contaminated soil having a selected contaminant. Such a system can include a plant growing in the contaminated soil, and contaminant-degrading mycobacteria colonized on a root of the plant, wherein the mycobacteria is capable of degrading the selected contaminant. The mycobacteria can be capable of degrading the selected contaminant, such as PAHs, PCPs, MTBEs, and the like. Additionally, the contaminant-degrading mycobacteria can be at least one of M. KMS, M. JLS, or M MCS. Also, the contaminant-degrading mycobacteria can have nid dioxygenase genes, which can further include a nidB-nidA sequence motif.

The invention relates to a manufacturing and cultivating method for a plant growth container, The method comprises the following steps: mixing materials which adopt plant fiber as the main materials and starch, additives and filling materials as auxiliary materials are hot-pressed in mould so as to generate the steam pressure swelling in the mould and a moulding foam container; internal sizing or waterproofing work for the whole surface and part of the surface is carried out; the method is used for culturing the plants in seedling stage, connecting plants, fixing planting, seeding the base and seeding together and different cultivating methods are used according to the degree of drought in the environment so as to realize water-saving and improve the survival rate of the plants; the method of the invention is beneficial to plant plants on a large scale by mechanization or air seeding, etc.

The present invention relates to applicators for applying powders to the vaginal mucosa.

The invention discloses an application of breast milk-derived lactobacillus reuteri in reducing fat and regulating fat metabolism rhythm, and belongs to the fields of microbial technology and food science. The lactobacillus reuteri FN041 can prevent and / or treat lipid metabolism rhythm disorder and has the functions particularly embodied in: (1) significantly reducing weight gain under the influence of high fat; (2) significantly reducing abnormal increase of peritesticular adipose tissue indexes under the influence of high fat; (3) significantly reducing hepatic fatty infiltration and abnormal increase of testicular fat cell area under the influence of high fat; (4) significantly decreasing abnormal increase of the content of TG, TC, LDL-C and HDL-C in serum under the influence of high fat; (5) significantly decreasing abnormal increase of the plasma FD4 content and the content of LPS, LBP and TNF-alpha in serum under the influence of high fat; and (6) restoring the content of triglyceride in serum and the rhythm of the expression of living clock genes Clock, Bmal1 and Per2 in liver under the influence of high fat.

The invention discloses a solid fermentation base applicable to the treatment of human, chicken, pig, cow and sheep excrement, crop straw and other solid organic waste for producing high-efficiency organic manure. Cultured solid bacteria agent is aired in an aseptic ventilating place; according to bacteria mixture ratio in percentage by weight, 32 percent of bacillus subtilis, 22 percent of bacillus licheniformis, 25 percent of bacillus megaterium, 7 percent of aspergillus oryzae and 14 percent of Absidia corymbifera are mixed into the solid fermentation base. As the solid fermentation base is added to livestock-poultry excrement, crop straw and other compost raw materials of which the moisture content and carbon nitrogen ratio are well regulated, aerobic microorganism in the raw materials can grow and reproduce fast, so as to rapidly raise the temperature of compost, shorten the fermentation period of the compost and realize harmless treatment; organic matter is transformed into a large amount of humic acid so as to raise the stability degree of the compost, which is beneficial to soil improvement, raises the crop yield and the quality, and reduces environmental pollution.