The invention discloses a lung tissue model for biotoxicity detection and a biotoxicity detection method. The lung tissue model comprises a trachea, a blood vessel and an alveolus pulmonis unit, wherein an air passage is defined in the trachea, and an air inlet and an air outlet, which are communicated with the air passage respectively are formed at two ends of the trachea; a blood passage is defined inside the blood vessel, and a liquid inlet and a liquid outlet which are communicated with the blood passage respectively are formed at the two ends of the blood vessel, a cavity is defined in the alveolus pulmonis unit, an elastic ventilate membrane is formed on the wall of the alveolus pulmonis unit, the alveolus pulmonis unit is arranged in the blood passage, and the cavity is communicated with the air passage. By adopting the lung tissue model, an air exchanging function and a breathing strain effect of the lung blood in the body can be simulated; through planting cells in the alveolus pulmonis unit, the structure and function of the alveolus pulmonis unit in a body and inflammation reaction of immune cells can be simulated in the subsequent culture process.