3 results about "Alveolus pulmonis" patented technology

The application provides a method for constructing a mouse emphysema model and application, and the method comprises specifically knocking out a PHLDA1 gene in type II alveolar epithelial cells in lung tissue of an animal. Compared with a traditional method for establishing an animal mouse emphysema model by exposing to cigarette smoke, the mouse emphysema model has good stability, can be bred, has a short modeling cycle, and better simulates the lung tissue pathological changes and pathophysiological changes of emphysema patients, and has high consistency with the patients.

The application discloses a kind of Sargassum fusiforme low molecular weight fucoidan, and its preparation method is as follows: S1, defatting treatment;S2, preparation of crude polysaccharide;S3, degradation of crude polysaccharide: Sargassum fusiforme fucoidan crude product is dissolved into polysaccharide solution with water, H2O2 is added, and degradation is carried out under ultraviolet light, to obtain Sargassum fusiforme low molecular weight fucoidan DSFF;S4, purification: Sargassum fusiforme low molecular weight fucoidan DSFF is dissolved into polysaccharide solution with water, loaded on DEAE-52 cellulose anion exchange column and eluted with NaCl solution, the eluate is concentrated and dialyzed, and freeze-drying is obtained.The application of the Sargassum fusiforme low molecular weight fucoidan in the preparation of drugs with anti-PM2.5 lung injury effect.The Sargassum fusiforme low molecular weight fucoidan of the application has low molecular weight, is easy to absorb, and has high bioavailability.The research of the application lays a foundation for the development of Sargassum fusiforme fucoidan related lung alveolus protection products.

The application belongs to the technical field of new application of medicines, and particularly relates to hydroxycinnamic acid derivatives, a preparation method and application thereof. The ARDS model is induced by LPS tracheal instillation combined with hydroxycinnamic acid derivatives, the alveolar lavage fluid cell count is determined, and the pathological characteristics and the expression level of inflammatory genes of lung tissues are detected; the results show that the hydroxycinnamic acid derivatives can significantly reduce lung cell infiltration, alleviate lung tissue damage and reduce the expression level of inflammatory factors.