33 results about "Rat blood" patented technology

The invention belongs to the field of biomedicine, and relates to a novel medicinal purpose of a traditional Chinese medicine effective component icariin. The invention specifically relates to a purpose of icariin in the preparation of medicines used for treating bronchial asthma. Test results show that, icariin assists in ameliorating asthma rat lung tissue inflammations, inhibiting inflammatory in asthma rat blood serum, inhibiting inflammatory in asthma rat alveolar lavage fluid, regulating expression unbalance of asthma rat lung tissue Th1 / Th2 relevant cytokine, and regulating expression unbalance of asthma rat lung tissue Th1 / Th2 relevant transcription factor (T-bet / GATA-3). The traditional Chinese medicine effective component icariin provided by the invention can be adopted as an active component, and can be prepared with pharmaceutically acceptable carriers into medicine compositions used for bronchial asthma.

The invention discloses a construction method of a diabetic cardiomyopathy animal model. An SD (Sprague Dawley) rat under high-sugar and high-fat feeding is subjected to multiple abdominal injection with STZ (streptozocin); after the diabetics diagnosis standard is reached, subcutaneous injection with isoprenaline is performed; after 2 weeks of continued feeding, left ventricular cannulation and three-lead electrocardiogram test of ventricular hemodynamic parameters and electrocardiogram parameters shows that the rat experiences cardiac dysfunction; heart weight index and left ventricular index show that the rat experiences evident ventricular hypertrophy; biochemical analysis on rat blood shows that the rat has evident myocardial damage; HE (hematoxylin-eosin) pathological staining satisfies pathological changes in diabetic cardiomyopathy; Masson collagen staining satisfies myocardial connective tissue proliferation of diabetic cardiomyopathy. The construction method has the advantages of good operational simplicity, short construction time, low mortality in construction phase, high construction success rate and the like, and the pathological process of myocardial damage due to human type 2 diabetes mellitus can be well simulated.

The invention discloses a Helichrysum arenarium Moench extractive as well as a preparation method and an application thereof. By a great quantity of animal experiments, the invention finds that the Helichrysum arenarium Moench extractive can effectively lower neutral fat content in animal hepatocyte, can obviously lower the content of total cholesterol and triglyceride in high blood fat rat blood and has the efficacy on effectively lowering blood fat. In addition, the Helichrysum arenarium Moench has the action on resisting atherosclerosis. The pharmacodynamics experiment shows that the Helichrysum arenarium Moench extractive can be used for the adjuvant therapy of obesity, high blood fat, atherosclerosis and relevant complications. The Helichrysum arenarium Moench extractive of the invention is characterized in that alcohols for Helichrysum arenarium Moench are heated and reflowed to extract and obtain an alcohol extractive; and after various auxiliary materials required by preparing different dosage forms and pharmaceutically acceptable carriers are added, the mixture is prepared into any one suitable clinic preparation with a conventional Chinese material medical preparation method.

The invention discloses a method for synchronously detecting in-vitro long-term exposed hemoglobin adduct for evaluating dietary acrylamide. The method comprises the chromatographic conditions and mass spectrum conditions of a blood (rat blood and human body blood) pretreatment method and a detection method. The method has the advantages that a UHPLC-MS / MS (ultra performance liquid chromatography-tandem mass spectrometry) method of isotope dilution is adopted, two types of hemoglobin adducts of the acrylamide are quantified, and the analysis time is greatly shortened, and AAVal-PTH and two types of space isomers of GAVal-PTH can be well separated at the same time; the synchronous detection is realized, and the internal exposure amount of the acrylamide can be more comprehensively evaluated.

The invention provides a preparation method of 9-bit-substituted double functional group berberine derivatives and belongs to the field of chemical synthesis. A pharmacological experiment proves that the derivatives have drug activity with a plurality of values and especially, the derivatives have the easy absorption performance which does not belong to a single function group drug. An animal experiment proves that the derivatives have excellent effects of adjusting hypertension rat blood pressure. The 9-bit-substituted double functional group berberine derivatives can prevent bonding of angiotensins II obtained through various approaches and their special acceptors so that blood pressure increasing effects are reduced or inhibited.

The invention belongs to the technical field of medicine, and particularly relates to a preparation method of a model for animals with living hypertension due to drinking. The model can be used for the screening, the pharmacological research and the pesticide effect evaluation of the medicine for treating the living hypertension. Through the adoption of the method that on the basis of a normal SD rat, the rat freely drinks alcohol everyday, (at first, drinking the alcohol with the volume concentration of 5 to 10 % for 3 to 7 days casually, then drinking more 2 to 5 % every one week until the volume concentration is 15 to 20 %; then drinking more 1 to 3 % every one week until the volume concentration is 21 to 25 %; then drinking by lasting the same concentration) for 8 to 17 weeks, the model for the animals with the living hypertension is created. The model screening indexes are mainly systolic pressure, diastolic pressure and mean pressure, and the systolic pressure is taken as a primary one; meanwhile the indexes in blood serum and urine are taken as supplements to be used for monitoring the influence of the long-term free drinking of the gradient concentration alcohol on the rat blood pressure and the functions of visceral organs such as a liver and a kidney.

The invention provides an LCMS / MS determination method of aloesin in rat blood plasma, which comprises the following steps: (1) blood plasma sample treatment: taking blood plasma to be detected containing aloesin, adding a methanol solution containing internal standard aloin D, carrying out vortex precipitation, centrifuging the mixture, and collecting the supernatant as a sample to be detected; (2) setting liquid chromatography conditions as follows: a mobile phase A and a mobile phase B are adopted for gradient elution, the mobile phase A is an aqueous solution containing 0.01-1.0% o (v / v) of formic acid, and the mobile phase B is methanol; and (3) setting mass spectrum conditions as follows: an electrospray ion source, negative ion detection, spray voltage of 4500V, spray temperature of550 DEG C, a multi-reaction ion monitoring scanning mode, an ion pair for quantitative analysis of aloesin of m / z 393.1 -> 272.9, and an internal standard compound aloin D ion pair of m / z 555.3 -> 144.9. The method provided by the invention can be used for detecting aloesin in blood plasma more quickly, sensitively and accurately.

The invention relates to an oral medicament for treating cerebral infarction and relieving limb spasm, and the oral medicament is prepared from the following traditional Chinese medicine active ingredients in parts by weight: 12-40 parts of membranous milkvetch root, 12-40 parts of pachyrhizua angulatus, 10-25 parts of red paeony root, 10-25 parts of szechuan lovage rhizome, 10-20 parts of peach seed, 10-25 parts of suberect spatholobus stem, 10-25 parts of common clubmoss herb, 10-25 parts of glandularstalk st.paulswort herb, 6-20 parts of twotooth achyranthes root and 3-10 parts of earthworm. Efficacy tests prove that the medicament can obviously inhibit the in-vivo blood vessel thrombosis of an animal, reduce the weight of in-vivo arterial and venous thrombus, inhibit the thrombosis and alleviate the clinical symptoms caused by the thrombus; and the medicament further has certain effect of improving coagulation function of rats, and can reduce the platelet aggregation rate, prolong the coagulation time, the PT (prothrombin time) and the APTT (activated partial thromboplastin time), have an obvious effect of improving blood rheology indicators, and can be used for significantly reducing blood viscosity of a rat blood stasis model caused by polymer dextran, improving the blood circulation, and avoiding the potential risk of the thrombosis.

The invention relates to health-care liquor with a uric acid reducing function and a preparation method thereof. The main effective constituent of the health-care liquor is phenylalanine lactic acid ester, and ingredients of the health-care liquor are liquorice and honey. The preparation method mainly comprises the steps that after the liquorice is added to liquor, liquorice liquor is obtained, the phenylalanine lactic acid ester and the honey are added to the liquorice liquor, the mixture is mixed and stirred, and the health-care liquor with the uric acid reducing function is obtained after filtering is conducted. The health-care liquor can increase the solubility of uric acid and is obvious in uric acid reducing effect. According to animal experiments in rats, the health-care liquor can obviously reduce the uric acid level of rat blood serum and has a certain kidney protection function; according to primary human feeding, the health-care liquor can obviously reduce the blood uric acid value of a patient suffering from hyperuricemia.

The invention discloses a human intron derived 27 base microRNA and an application thereof to blood pressure regulation. The human intron derived 27 base microRNA is generated by four-time duplicationof a fourth intron 27 base duplicon of eNOS (endothelial nitric oxide synthase), and the nucleotide sequence of the human intron derived 27 base microRNA is as shown in SEQ ID NO. 1. A high-expression plasmid is prepared according to the sequence, the high-expression plasmid and lipidosome are mixed according to the weight ratio of 3:1 and diluted by normal saline, and mixture is injected into the body of an SD (sprague-daw) rat through rat caudal veins to prove that the human intron derived 27 base microRNA has a regulating effect on rat blood pressure, can be used for researching hypertension diseases, finally can be used for screening some antihypertensive drugs and preparing a hypertension early warning chip and has a potential application prospect.

The invention discloses a needle door type mousetrap cage, which comprises an outer cage and an inner groove assembly mounted on the inner top surface of the outer cage, wherein the inner groove assembly is hung on the upper top surface of the inner side of the outer cage and the length of the inner groove assembly is smaller than that of the outer cage; the inner groove assembly comprises an inner groove body, a rotary needle door, a rocker plate and a rear hanging door; and the rotary needle door is mounted at an opening at the front end of the inner groove body, an opening is formed in an area, which faces the rotary needle door, of the outer cage, the rocker plate is mounted at the front part of a bottom plate of the inner groove body through a torsion spring device, a linkage device is arranged at the bottom of the rocker plate, the linkage device drives the rotary needle door to open and close, the rear hanging door is hinged to the top and bottom ends of the side surface at therear section of the inner groove body, and a one-way blocking device is arranged on the rear hanging door. The cage has no chemical agent, no electricity, safe mousetrap process, no rat blood stains on the ground or other places, modular design for easy disassembly and mounting, can realize automatic continuous mousetrap, can be used for large-scale industrial production and is convenient to popularize.

The invention relates to a building method of a stagnant heat interties animal model for screening medicaments for treating stagnant heat interties, evaluating the traditional Chinese medicine property and researching the traditional Chinese medicine theory. The method comprises the following steps of: selecting a proper rat, injecting carrageenin into the abdominal cavity of the rat for 3 days continuously, and 24 hours later, perform hypodermic injection of dry yeast solution into the back part of the rat. The symptoms of a rat model built by the invention are similar to clinical symptoms, namely, the mouse has the symptoms of purpureal tongue, longer and thicker tongue bottom venation, purpureal onyxes, obvious thrombus on the tail part, body temperature increasing and the like, at the same time, hemorheology and microcirculation detection show that the rat blood viscosity is obviously increased and the blood flow speed is obviously reduced. The model has the advantages of good repetitiveness and stability, and capability of withstanding a test with medicaments.

The invention discloses an application of equol-based BKCa channel activation. Pharmaceutical activity of equol is detected on the basis of applying a rat focal cerebral ischemia reperfusion model, a tail-cuff method to measure rat blood pressure, a Laser Doppler Flowmetry to observe brain parenchyma and pia mater blood flow, an in-vitro vascular muscle tension tracing and a patch clamp technology, and results show that the equol, through activating beta1 subunit of BKCa channel, can dilate blood vessels, increase cerebral blood flow and protect ischemic reperfusion brain tissue, is beneficial for prevention and treatment of ischemia brain stroke, and can be used for preparation of related medicaments.

The invention belongs to the technical field of medicines, and particularly relates to a traditional Chinese medicine composition with blood enriching effects of qi and blood tonifying and a preparation method thereof. The traditional Chinese medicine composition is prepared from, by mass, 200g of ginseng, 120g of angelica sinensis, 60g of prepared fleece flower roots, 30g of ligusticum wallichiiand 30g of liquorice. The preparation method of the traditional Chinese medicine composition comprises the following steps of (1) treating 120g of the angelica sinensis and 30g of the ligusticum wallichii to extract volatile oil; (2) treating 200g of the ginseng, 60g of the prepared fleece flower roots and 30g of the liquorice, and extracting pure creams; (3) preparing capsules. A large number ofanimal experiments prove that the traditional Chinese medicine composition has remarkable improvement on the blood deficiency condition of a rat blood deficiency model, and has a good effect on clinically treating qi-blood deficiency syndromes.

The invention belongs to the technical field of medicine, and relates to a method for evaluating the pharmacological activity of traditional Chinese medicine in vivo. The specific technical scheme is: after administering the traditional Chinese medicine to experimental animals, evaluate the changing trend of pharmacological indicators, and collect and administer the medicine at the best time for the action of the traditional Chinese medicine. The biological fluids of animals are reinjected into the experimental animals after appropriate treatment, and then the change trend of the pharmacological indicators is evaluated and compared with the data obtained after direct administration of traditional Chinese medicine to evaluate the time-lag effect of traditional Chinese medicine on the pharmacological indicators. The invention combines the whole pharmacokinetics of the traditional Chinese medicine based on the modern analysis technology to evaluate the effective components and the synergistic or antagonistic components in the effective substances of the traditional Chinese medicine.

The invention discloses a novel purpose of pteris multifida poir general flavone in preparation of medicine for treating hyperlipemia and atherosclerosis. The lipid-lowering and anti-atherosclerosis effects are studied through a rat lipid metabolism disturbance model and an atherosclerosis model; study results prove that the pteris multifida poir general flavone can reduce the contents of TG, CHO and LDL-C in the rat blood serum; the H / L ratio is increased; the atherosclerotic plaque length and area can be reduced; good lipid-lowering and anti-atherosclerosis effects are achieved; the pteris multifida poir general flavone can be prepared into medicine applied to the aspect.

The invention discloses a high-efficiency alcohol effect dispelling and liver protecting composition, and a preparation method and an application thereof. The composition comprises, by weight, 280-390 parts of dehydrated Ampelopsis grossedentata leaves, 210-580 parts of Hovenia acerba Lindl., 140-300 parts bof corn oligopeptide powder and 90-130 parts of Acai instant powder, wherein the dehydrated Ampelopsis grossedentata leaves undergoes ultrasonic extraction, the content of total flavonoids in the obtained extract is not less than 35%, and the content of dihydromyricetins is 25-32%; the Hovenia acerba Lindl. undergoes ethanol refluxing extraction, and the content of total flavonoids in the obtained extract is not less than 8%; and the Acai instant powder is prepared through Acai beating, filtering for residue removal, filtrate concentration, auxiliary material adding, and mixing and drying. A result of test shows that the composition significantly degrades ethanol in rat blood, continuously enhances the activity of ADH in liver of mice, effectively prevents alcohol-induced liver GSH depletion and MDA increase, reduces the TG content, and has good alcoholic liver injury prevention and treatment effects.

The invention discloses a traditional Chinese medicine composition for promoting fracture healing. Traditional Chinese medicine or medicine and food homologous medicines including notopterygium roots,radix angelicae pubescentis, safflower carthamus, Chinese angelica, myrrh, mastic, peach kernel, ground beeltle, pseudo-ginseng, dragon's blood, lumbricus, the bark of himalayan coralbean, scardiniuserythrophthalmus, sinomenium acutum, sinomenia sinensis, vernonia gratiosa, beautiful millettia roots, horsetail, artemisia apiacea, oldenlandia diffusa, gypsum, gastrodia elata, sculellaria barbata,homalomena occulta and the like are used as raw materials; the traditional Chinese medicine composition has good recovery effect on fracture; the traditional Chinese medicine composition does not have toxic and side effects to human bodies, can invigorate the circulation of blood, eliminate addiction, dredge stagnation blood, disperse swelling, settle pain, regulate the flow of vital energy and enlighten the mind, can rapidly relieve swelling of a rat blood part, can promote porosis, and can promote increasing of healing power of bone and tendons at the fracture part; various traditional Chinese medicine raw materials in the traditional Chinese medicine composition in the invention are used in a matched manner; a synergistic effect is achieved; the formation effect of poroma can be effectively improved; formation of poroma can be easily promoted; the fracture healing time is greatly reduced; and thus, the traditional Chinese medicine composition in the invention has wide market application prospect.

The invention concretely relates to a method for simultaneously detecting paracetamol and its eight blood plasma metabolites. An experiment result shows that the method allows the lower quantification limit of the paracetamol and its eight blood plasma metabolites to reach 0.46 ng / mL, and the correlation coefficient of every linear regression equation is 0.998; the intra-day accuracy of all detected substances is 85-111%, and the precision is lower than 9%; and the inter-day accuracy of all the detected substances is 94-108%, and the precision is lower than 9%. The method is fast, sensitive and accurate, and is suitable for quantitatively detecting the paracetamol and its eight blood plasma metabolites in rat blood plasma.

The invention discloses a traditional Chinese veterinary medicine composition or an animal food additive for preventing and / or treating kidney diseases, and belongs to the field of medicines. The traditional Chinese veterinary medicine composition is a preparation prepared from the following raw material medicines in parts by weight: 2.8-3.2 parts of rheum officinale and 6.8-7.2 parts of salvia miltiorrhiza. Compared with salvia miltiorrhiza or rheum officinale with the same dosage, the traditional Chinese veterinary medicine composition has an obvious better effect of reducing creatinine and urea nitrogen in rat blood, so that the traditional Chinese veterinary medicine composition consisting of salvia miltiorrhiza and rheum officinale in a specific ratio plays a synergistic role in preventing acute kidney injury and protecting kidney functions. In addition, the traditional Chinese veterinary medicine composition can effectively reduce the levels of blood creatinine and urea nitrogen of cats with chronic renal failure, and can effectively treat chronic renal failure. The traditional Chinese veterinary medicine composition is composed of salvia miltiorrhiza and rheum officinale according to a specific ratio, the medicines are simple and easy to obtain, the preparation process is simple, and the application prospect is wide.

The invention relates to the technical field of medicines and in particular relates to application of a triterpenoid saponins compound. The invention provides application of the triterpenoid saponins compound with a structure formula I to preparation of a medicine for treating asthma. An experiment testifies that the triterpenoid saponins compound with the structure formula I has an obvious inhibition effect on a platelet activating factor receptor and can be used for alleviating tachypnea and accelerated frequency, caused by the asthma, and remarkably reducing the amount of Eos (Eosinophile granulocyte) in rat blood and reducing infiltration on trachea by the Eos; meanwhile, the secretion of GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) in BALF (Bronchoalveolar Lavage Fluid) of guinea pigs can be inhibited and a PAF (Platelet-Activating Factor) level of blood serum of the guinea pigs is remarkably reduced, and the activation of inflammatory cells and the releasing of inflammatory transmitters are stopped, so that inflammation of the trachea is alleviated and smooth muscles of bronchus are relaxed; the high responsiveness of the trachea is reduced to treat the asthma, so that the triterpenoid saponins compound has the effect of treating the asthma and application value.

The invention relates to the technical field of biomedicine, in particular to an evaluation method of ADSCs for relieving filial generation rat hypertension and application. The method comprises the following steps of (1) cell culture: putting the ADSCs into a complete culture medium for culture; (2) cell marking: diluting 1mg / ml SPIO by a complete culture medium into 50 mug / mL; meanwhile, diluting polylysine into 1.5ug / ml; then mixing the two kinds of solutions according to a ratio of 1:1, wherein the final dilution concentration of the polylysine is 0.75 mug / mL; after the sufficient and uniform mixing, putting the materials into a cell culture box to be cultured; (3) pregnancy period modeling: building an LPS group, an LPS+ADSCs group and a control group (for the LPS group, injecting LPSinto the abdominal cavity of SD pregnant rats on the 8th day, 10-th day and 12-th day from the pregnancy; for the LPS+ADSCs group, injecting LPS into the abdominal cavity of SD pregnant rats on the 8th day, 10-th day and 12-th day from the pregnancy; meanwhile, injecting the ADSCs prepared in the step 2 into the caudal vein on the 8th day from the pregnancy; for the control group, injecting the equal volume normal saline into the abdominal cavity of SD pregnant rats on the 8th day, 10-th day and 12-th day from the pregnancy); 4, SPIO-ADSCs migration observation under MRI; (5) filial generation rat blood pressure change observation.

The invention relates to detection of drug metabolism in blood plasma, in particular to a method for detecting five ginsenosides in rat blood plasma, which is characterized by comprising the followingsteps of precipitating protein by using methanol, extracting by using dichloromethane, purifying by using a solid-phase extraction column, and detecting Ra1, Rb1, Rh1, Re and Ro in the blood plasma by using gas chromatography. Compared with the prior art, the method has the advantages of high accuracy, high sensitivity and good repeatability, is suitable for simultaneous quantitative detection ofthe five ginsenosides in the blood plasma, and can be used for research on metabolic pathways of the ginsenosides in the blood plasma.

The invention discloses a blood glucose reducing and islet protecting activity test method for a horseradish tree leaf flavonoids extract and application thereof. The blood glucose reducing and islet protecting activity test method for the horseradish tree leaf flavonoids extract comprises the following steps: S1, preparing a flavonoids extract; S2, glucose consumption test: carrying out glucose consumption test according to a glucose consumption activity coefficient K; S3, glucolipid metabolism activity test: calculating a rat blood lipid index, measuring rat serum SOD and MDA contents and calculating an insulin index; and S4, islet protecting activity test: calculating islet cell quantity change rate M and islet beta cell growth rate N. By the method, the glucose consumption activity coefficient K is calculated, the blood glucose change rate of rats is measured, and the rat blood lipid index, the rat serum SOD and MDA contents, the insulin index, the islet cell quantity change rate M and the islet beta cell growth rate N are measured, the blood glucose reducing and islet protecting activity index of the horseradish tree leaf flavonoids extract is judged comprehensively, and therefore, a quantitative standard is provided for utilization and development of horseradish tree leaves.