Building method of stagnant heat interties animal model

An animal model, the technology of blood stasis and heat intertwining, applied in the direction of pharmaceutical formulas, preparations for in vivo experiments, etc., can solve problems such as direct evidence, multiple deaths, and large individual differences in endotoxin reactions

Inactive Publication Date: 2015-07-22
邓家刚
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Problems solved by technology

[0004] First of all, after using model animals to be infected by bacteria, although the dose or route of attacking the virus is changed, it is often still difficult to control the process of the disease, and eventually many people die, and the mortality rate is relatively high. the result of
Moreover, it is difficult to accurately control the dosage of bacteria as attacking materials.
[0005] The second is to use endotoxin as the attacking material. The condition and pathological process of modeled animals can be easily controlled by changing the dosage or the time of medication. Changes in indicators cannot be used as direct evidence of blood stasis, such as low coagulation, low poly, and other phenomena that do not directly conform to the characteristics of blood stasis
[0006] Thirdly, the individual differences in the response of rats to endotoxin are large, and the stability of the model is poor

Method used

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  • Building method of stagnant heat interties animal model
  • Building method of stagnant heat interties animal model
  • Building method of stagnant heat interties animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Select 24 SD rats, SPF grade, and randomly divide them into normal group and model group, 12 rats in each group, kept in a clean animal room, room temperature 20±2°C, intraperitoneal injection of 10mg / mL carrageenan in model group rats, continuous 3 days, 24 hours after the last injection of carrageenan, the rats were subcutaneously injected with 20% dry yeast solution on the back, and the anal temperature of the rats was measured 6 hours later. Afterwards, they were anesthetized with 10% chloral hydrate and placed in a cold light source shooting box. Collect images of mouse fur, auricles, limbs, tail, and tongue, and measure and record the total length of the tail and the length of the thrombus. Rheological determination.

[0015] Experimental results:

[0016] 1. Overall signs of TCM

[0017] Rats in the model group had reddish-purple tongue, red auricles, thickened sublingual veins, bruised nails, obvious thrombus in the tail, and elevated body temperature.

[001...

Embodiment 2

[0030] Select 36 SD rats, SPF grade, and randomly divide them into normal group, model group, and Danshen group, with 12 rats in each group, and raise them in a clean animal room at a room temperature of 20±2°C; 2 times / day, for 7 consecutive days, the normal group and the model group were given the same volume of distilled water; on the 5th day of intragastric administration, the rats in the model group and the Danshen group were intraperitoneally injected with carrageenan at a concentration of 10 mg / mL for 3 consecutive days, and the last injection After 24 hours of carrageenan, the rats were subcutaneously injected with 20% dry yeast solution on the back, and the anal temperature of the rats was measured 6 hours later. After that, they were anesthetized with 10% chloral hydrate and placed in a cold light source shooting box. , Limbs, Tail, Tongue for image collection, and measure and record the length of the tail and the length of the thrombus, and then put it in a microcirc...

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Abstract

The invention relates to a building method of a stagnant heat interties animal model for screening medicaments for treating stagnant heat interties, evaluating the traditional Chinese medicine property and researching the traditional Chinese medicine theory. The method comprises the following steps of: selecting a proper rat, injecting carrageenin into the abdominal cavity of the rat for 3 days continuously, and 24 hours later, perform hypodermic injection of dry yeast solution into the back part of the rat. The symptoms of a rat model built by the invention are similar to clinical symptoms, namely, the mouse has the symptoms of purpureal tongue, longer and thicker tongue bottom venation, purpureal onyxes, obvious thrombus on the tail part, body temperature increasing and the like, at the same time, hemorheology and microcirculation detection show that the rat blood viscosity is obviously increased and the blood flow speed is obviously reduced. The model has the advantages of good repetitiveness and stability, and capability of withstanding a test with medicaments.

Description

technical field [0001] The invention belongs to the technical field of medical evaluation and theoretical research of traditional Chinese medicine, and in particular relates to an animal model and a method for establishing the same for screening and treating drugs for stasis-heat syndrome, evaluating the properties of traditional Chinese medicine and studying the theory of traditional Chinese medicine. Background technique [0002] The so-called stasis-heat syndrome refers to that at a certain stage of the development of acute exogenous febrile diseases or internal injuries and miscellaneous diseases, fire-heat toxins or phlegm-dampness are blocked in the blood, and the blood is blood stasis, causing blood heat and blood stasis. A syndrome formed by pathological factors combining with each other and causing trouble (Zhou Zhongying, On Stasis Heat, Journal of Nanjing University of Traditional Chinese Medicine, 2006, 22(5): 273-276.). Based on TCM theory and many years of clin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00
Inventor 邓家刚杨柯郝二伟杜正彩包传红冯旭
Owner 邓家刚
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