Application of triterpene saponins
A technology of triterpene saponins and compounds, applied in the field of medicine
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Embodiment 1
[0057] Example 1 Ecliptasin A, B, C and D inhibit activity of platelet activating factor receptor (PTAFR)
[0058] 1. Experimental materials:
[0059] 1.1 Cell line: The cell line CHO-K1 stably transfected with PTAFR was constructed by Jiangsu Kangyuan Pharmaceutical Co., Ltd.
[0060] 1.2 Main reagents: F12 medium and fetal bovine serum were purchased from Hyclone Company; Zeocin and HBSS were purchased from Invitrogen Company; calcium flux detection kit was purchased from Molecular Device Company; PAF (C16), WEB2086 were purchased from tocris; HEPES was purchased from Nanjing Shengxing Biotechnology Co., Ltd.; DMSO was purchased from sigma;
[0061] 1.3 Instruments: FLIPR tetra microplate reader, Molecular Devices; pipette, eppendorf; ultra-clean bench, purchased from Shanghai Jiejia Air Purification Technology Co., Ltd.; CDX-K1 inverted microscope, purchased from Olympus; countess The automatic cell counting instrument was purchased from Invitrogen Company; the 3111 cell ...
Embodiment 2
[0080] Embodiment 2Eclipta glucoside A, B, C and D are to the influence of bronchial asthma rat model
[0081] 1. Experimental materials:
[0082] 1.1 Animals: clean grade SD rats, all male, with a body weight of 260±20g, from Qinglongshan Animal Breeding Farm.
[0083] 1.2 Main reagents: Ovalbumin (OVA) was purchased from Sigma Company; Dexamethasone Sodium Phosphate Injection (5 mg / m1) was purchased from Guangzhou Baiyunshan Mingxing Pharmaceutical Co., Ltd.
[0084] 1.3 Instruments: Powerlab / 8sp' physiological signal acquisition system, purchased from Ed Instruments International Trade (Shanghai) Co., Ltd.; LDZ5-2 centrifuge, purchased from Beijing Jingli Centrifuge Co., Ltd.; M2e microplate reader, purchased from MolecularDevices Company; medical ultrasonic atomization inhaler, Shanghai Medical Equipment Factory.
[0085] 2. Experimental method:
[0086] 2.1 Preparation of asthma model: 1ml of 10% OVA, 100mg of aluminum hydroxide dry powder, inactivated Bacillus pertuss...
Embodiment 3
[0096] The effect of embodiment 3 Eclipta glucoside A, B, C and D on the therapeutic effect of asthmatic guinea pig GM-CSF and PAF
[0097] 1. Experimental materials:
[0098] 1.1 Animals: 120 healthy male variegated guinea pigs, body weight 350-450g, Qinglongshan Animal Breeding Farm. 1.2 Main reagents: Ovalbumin (OVA) was purchased from sigma; GM-CSF detection kit was purchased from Shanghai Kexing Biotechnology Company; PAF ELISA detection kit was purchased from Shanghai Meixuan Biotechnology Co., Ltd.; positive control drug Aminophylline tablet, specification: 0.1g / sheet, product of Shanxi Yunpeng Pharmaceutical Co., Ltd.
[0099] 1.3 Instruments: LDZ5-2 centrifuge, purchased from Beijing Jingli Centrifuge Co., Ltd.; M2e microplate reader, purchased from Molecular Devices; medical ultrasonic atomizing inhaler, Shanghai Medical Device Factory.
[0100] 2. Experimental method:
[0101] 2.1 Model replication and drug administration 120 guinea pigs were randomly divided int...
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