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Preparation method of model for animals with living hypertension due to drinking

An animal model, high blood pressure technology, applied in the field of medicine, can solve problems such as no animal model

Inactive Publication Date: 2013-11-06
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the common hypertensive rat models in the laboratory include spontaneously hypertensive rats, two-kidney-one-clip hypertensive rat models, etc., but no animal models close to the pathogenesis of human hypertension have been seen. The present invention is based on daily life , the cause of human hypertension, such as long-term alcohol consumption of a certain amount of alcohol, established an animal model that can be used for screening, pharmacological research and drug efficacy evaluation of drugs for the treatment of living hypertension

Method used

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  • Preparation method of model for animals with living hypertension due to drinking
  • Preparation method of model for animals with living hypertension due to drinking
  • Preparation method of model for animals with living hypertension due to drinking

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Embodiment 1

[0007] 1 Experimental materials and instruments

[0008] 1.1 Animals SD rats, half male and half male, weighing 180-220 g, 20 rats. License No. SCXK (Zhejiang) 20080033, provided by Zhejiang Experimental Animal Center.

[0009] instrument ALC-NIBP non-invasive blood pressure measurement and analysis system (Shanghai Alcott Biotechnology Co., Ltd.); Holland Ritu SELECTRA-E automatic biochemical analyzer (Netherlands Ritu company); Powerwave340 microplate reader (US Bio-TEK company); DH -500K / Na / Cl / Ca / pH electrolyte analyzer (Shenzhen Baikangli Technology Co., Ltd.).

[0010] Reagent Alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), uric acid (UA), creatinine (Cr) (Shanghai Fosun Long March Medical Science Co., Ltd.); A Calibration / Washing Solution, B Slope Calibration Solution, Protein-Removing Cleaning Solution, manufactured by Wuhan Zhongda Biosensing Technology Co., Ltd.

[0011] exp...

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Abstract

The invention belongs to the technical field of medicine, and particularly relates to a preparation method of a model for animals with living hypertension due to drinking. The model can be used for the screening, the pharmacological research and the pesticide effect evaluation of the medicine for treating the living hypertension. Through the adoption of the method that on the basis of a normal SD rat, the rat freely drinks alcohol everyday, (at first, drinking the alcohol with the volume concentration of 5 to 10 % for 3 to 7 days casually, then drinking more 2 to 5 % every one week until the volume concentration is 15 to 20 %; then drinking more 1 to 3 % every one week until the volume concentration is 21 to 25 %; then drinking by lasting the same concentration) for 8 to 17 weeks, the model for the animals with the living hypertension is created. The model screening indexes are mainly systolic pressure, diastolic pressure and mean pressure, and the systolic pressure is taken as a primary one; meanwhile the indexes in blood serum and urine are taken as supplements to be used for monitoring the influence of the long-term free drinking of the gradient concentration alcohol on the rat blood pressure and the functions of visceral organs such as a liver and a kidney.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for preparing an animal model of alcohol-induced active hypertension. The model can be used for screening, pharmacological research and drug effect evaluation of drugs for treating active hypertension. Background technique [0002] There are many causes of high blood pressure in the human body. Studies have shown that high-concentration alcohol can cause arteriosclerosis, produce and aggravate high blood pressure, and damage cardiovascular and cerebrovascular. Excessive alcohol consumption is an independent risk factor for cardiovascular disease, and alcohol consumption can lead to kidney damage. The kidney is an important organ of the human body, and directly participates in the occurrence of essential hypertension through the regulation of body fluids and the production of vasoactive substances. Moreover, high blood pressure is an important independent ri...

Claims

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Application Information

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IPC IPC(8): A01K67/027
Inventor 吕圭源陈素红苏洁夏超群
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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