7 results about "Essential hypertension" patented technology

The application provides a marker for diagnosing primary aldosteronism, a model and application thereof, and combines a univariate analysis and a logistic regression analysis method, analyzes primary aldosteronism samples and primary hypertension samples in a national multicenter cohort, screens out a marker that can be used for predicting whether an individual has primary aldosteronism, and further utilizes a decision tree algorithm to construct a prediction model, realizes intelligent, rapid and simple prediction and diagnosis of primary aldosteronism based on LC-MS / MS technology and the prediction model, and meets clinical requirements.

This application belongs to the field of immunoassay technology, and discloses an aldosterone sandwich antibody mAb5 or its antigen-binding fragment, its preparation method, and its applications. The aldosterone sandwich antibody mAb5 or its antigen-binding fragment includes a light chain variable region (VL) and a heavy chain variable region (VH). The light chain variable region (VL) includes complementarity-determining regions (LCDR1, LCDR2, and LCDR3), whose amino acid sequences are shown in SEQ ID NO. 1-3, respectively. The heavy chain variable region (VH) includes complementarity-determining regions (HCDR1, HCDR2, and HCDR3), whose amino acid sequences are shown in SEQ ID NO. 4-6, respectively. Using the aldosterone sandwich antibody mAb5 of this application to detect aldosterone standard antigens, the detection sensitivity is less than 7 pg / mL. Magnetochemiluminescence immunoassay of clinical samples shows good correlation with clinical results within the sample range of 0-1000 pg / mL, which is of great significance in the diagnosis and treatment of essential hypertension and other aldosterone-related diseases.

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition for reducing blood pressure and a preparation method thereof. The product provided by the invention is suitable for primary hypertension patients, especially patients who have poor tolerance to chemical antihypertensive drugs and worry about adverse reactions or prefer to traditional Chinese medicine treatment. Patients orally take the traditional Chinese medicine composition according to recommended doses every day, and the effect of reducing blood pressure is achieved through the synergistic effect of all the components in the traditional Chinese medicine composition (for example, the chrysanthemum bud has the effect of clearing heat and calming the liver, the radix puerariae has the effect of clearing and activating the channels and collaterals, the earthworm has the effect of clearing heat and arresting convulsion, the eucommia ulmoides has the effect of tonifying liver and kidney and strengthening muscles and bones, and the radix paeoniae alba has the effect of nourishing blood and liver).

The invention belongs to the technical field of immunodetection, and discloses an aldosterone sandwich method antibody mAb7 or an antigen binding fragment thereof, and a preparation method and application thereof. The aldosterone sandwich method antibody mAb7 or the antigen binding fragment thereof comprises a light chain variable region VL and a heavy chain variable region VH, the light chain variable region VL comprises complementary determining regions LCDR1, LCDR2 and LCDR3, the amino acid sequences of the light chain variable region VL and the heavy chain variable region VH are respectively shown as SEQ ID NO.1-3, the heavy chain variable region VH comprises complementary determining regions HCDR1, HCDR2 and HCDR3, and the amino acid sequences of the heavy chain variable region VH are respectively shown as SEQ ID NO.4-6. When the aldosterone sandwich method antibody mAb7 is used for detecting aldosterone standard antigens, the detection sensitivity is lower than 10 pg / mL, a clinical sample is detected through a magnetochemiluminescence method, the correlation with R clinical comparison is good in the sample range of 0-1000 pg / mL, and the aldosterone sandwich method antibody mAb7 has important significance in the field of diagnosis and treatment of primary hypertension and other aldosterone abnormality related diseases.

The present invention belongs to the field of molecular diagnosis of medical diseases and their treatment. More specifically, the present invention provides methods and means for detecting hypertension, more specifically essential primary hypertension, and even more specifically NOX5-dependent hypertension. The present invention also provides methods for treating hypertension, more specifically essential primary hypertension, and more specifically NOX5-dependent hypertension. The present invention also provides theragnostics combining therapy with diagnosis, more specifically, determining the level of NOX5 in a sample from a subject, and treating the subject with a NOX5 inhibitor or a compound that reduces the level of NOX5 if the NOX5 level exceeds a certain threshold. Furthermore, the present invention also provides theragnostics combining diagnosis with therapy, more specifically, determining the level of NOX5 (plasma) in a sample from a subject, and treating the subject with a NOX5 inhibitor or a compound that reverses the consequences of NOX5 activity if the determined NOX5 level exceeds a predetermined threshold. Finally, the present invention relates to animal models suitable for developing diagnostic methods and therapeutic treatments for NOX5-dependent hypertension.

The disclosure relates to a combination of biomarkers comprising at least: (i-a) one biomarker selected in each of the following group of biomarkers: Patient's age, Plasma steroids, and Urinary steroids, and at least one biomarker selected in at least one of the group of biomarkers: O-methylated catecholamines, Small metabolites, and miRNA; or (i-b) one biomarker selected in each of the following group of biomarkers: Plasma steroids, Urinary steroids, and Small metabolites, and at least one biomarker selected in at least one of the group of biomarkers: Patient's age, O-methylated catecholamines, and miRNA. The combinations of biomarkers may be used for stratifying a hypertensive patient among different hypertensive diseases comprising Endocrine Hypertension (EHT), Primary Aldosteronism (PA), Pheochromocytoma / Functional Paraganglioma (PPGL), Cushing's Syndrome (CS), and Primary Hypertension (PHT).

The invention discloses a losartan potassium pharmaceutical composition as well as a preparation method and application thereof. The losartan potassium pharmaceutical composition comprises a eutectic compound formed by losartan potassium and arginine and polyethylene glycol with the molecular weight of 4000. Through the synergistic effect of a co-crystallization technology and specific auxiliary materials, the solubility and stability of losartan potassium are remarkably improved, and the dissolution rate of losartan potassium in a hydrochloric acid medium with the pH value of 1.2 for 15 min reaches up to 85% or above and is far superior to that of commercially available common tablets. Animal experiments show that the relative bioavailability of the pharmaceutical composition can reach more than 200% of that of a commercially available reference preparation, so that low-dose efficient treatment is realized, and adverse reactions are reduced. The composition has excellent stability under high-temperature and high-humidity conditions, is suitable for preparing medicines for treating primary hypertension and related cardiovascular diseases, and has important clinical value.