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Enzymatic preparation method for antihypertensive peptides from jellyfish

A technology of enzymatic preparation and antihypertensive peptide, applied in fermentation and other directions, can solve problems such as jellyfish antihypertensive peptides that have not yet been seen, and achieve the effects of controllable product quality, strong operability and high activity

Active Publication Date: 2011-09-21
SHANDONG HOMEY AQUATIC DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the preparation of jellyfish antihypertensive peptide by double-enzyme hydrolysis

Method used

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  • Enzymatic preparation method for antihypertensive peptides from jellyfish

Examples

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Comparison scheme
Effect test

Embodiment 1

[0020] (a) Processing of raw materials Weigh 500kg of fresh jellyfish as raw material, soak in clear water to desalinate until the salt content is 3%, after cleaning, use a colloid mill to grind, and the particle size of the obtained jellyfish pulp is controlled at 3.5um;

[0021] (b) The first step of enzymatic hydrolysis to measure the moisture and protein content of the jellyfish pulp obtained in step (a), according to the measurement results, add water to adjust the protein mass concentration to 2%; add acid to adjust the pH value to 3.26, and control the temperature to 37°C , add pepsin, the dosage of pepsin is to add 3000u pepsin per gram of protein, keep warm for 4 hours, and get jellyfish pepsin hydrolyzate;

[0022] (c) In the second step of enzymatic hydrolysis, the pH value of the jellyfish pepsin hydrolyzate obtained in step (b) is adjusted to 5.02 with an alkaline solution, the temperature is raised to 50° C., and papain is added in an amount of 4000 u per gram of ...

Embodiment 2

[0027] (a) Processing of raw materials Weigh 500kg of alum-made jellyfish as raw material, soak in clean water, rehydrate, desalinate until the salt content is less than 5%, clean it with running water, and then use a colloid mill to grind it. The particle size of the obtained jellyfish pulp is controlled at 2um ;

[0028] (b) The first step of enzymatic hydrolysis to measure the moisture and protein content of the jellyfish pulp obtained in step (a), according to the measurement results, add water to adjust the protein mass concentration to 3%; add acid to adjust the pH value to 2, and control the temperature to 35°C , add pepsin, the dosage of pepsin is to add 4000u of pepsin per gram of protein, heat preservation enzymolysis for 5h, get jellyfish pepsin hydrolyzate;

[0029] (c) The second step of enzymatic hydrolysis Adjust the pH of the jellyfish pepsin hydrolyzate obtained in step (b) to 6 with an alkaline solution, control the temperature at 55°C, add papain, the amount...

Embodiment 3

[0034] (a) Processing of raw materials Weigh 300kg of jellyfish made from alum as raw material, soak in clear water, rehydrate, desalinate until the salt content is less than 4%. After washing with running water, use a colloid mill for grinding treatment, and the particle size of the obtained jellyfish pulp is controlled at 5um;

[0035](b) The first step of enzymatic hydrolysis determination of the moisture and protein content of the jellyfish pulp obtained in step (a), adding water to adjust the protein mass concentration to 1%; adding acid to adjust the pH value to 3, controlling the temperature to 40°C, adding pepsin, The dosage of pepsin is to add 2000u of pepsin per gram of protein, and keep it warm for 6 hours to get jellyfish pepsin hydrolyzate;

[0036] (c) The second step of enzymatic hydrolysis Adjust the pH value of the jellyfish pepsin hydrolyzate obtained in step (b) to 5.5 with an alkaline solution, control the temperature at 52 ° C, add papain, and the amount of...

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Abstract

The invention relates to an enzymatic preparation method for antihypertensive peptides from jellyfish, which comprises: selecting fresh jellyfish or jellyfish processed with vitriol as raw materials, desalting, washing and grinding; regulating the protein concentration of jellyfish pulp to 1 to 3 percent; regulating pH value to 2 to 3.5, controlling the temperature to be 35 to 45 DEG C, adding pepsase, and keeping temperature to perform enzymolysis for 4 to 6 hours, wherein the added amount of the pepsase per gram of protein is 2,000 to 4,000um; regulating the pH value of the pepsase hydrolysate of jellyfish to 5 to 6, keeping the temperature between 50 and 55 DEG C, and adding papain to perform enzymolysis for 1 to 3 hours, wherein the added amount of papain per gram of protein is 2,000 to 4,000mu; and deactivating enzymes; and filtering with a membrane for removing impurities, desalinizing by a nanofiltration membrane, concentrating, spray-drying or freeze-drying to obtain powder of antihypertensive peptides from jellyfish. The antihypertensive peptides from jellyfish, which are prepared by the method, are proved in spontaneously hypertensive (SHR) rat oral taking experiments to have remarkable blood pressure reduction assisting effect. In the invention, the production process is advanced, the operability is high, the enzymolysis of the protein is complete, and the product quality is controllable.

Description

technical field [0001] The invention relates to a general preparation method of peptides, in particular to an enzymatic preparation method of jellyfish antihypertensive peptide obtained by enzymatically hydrolyzing jellyfish collagen peptide liquid, and then separating, concentrating and drying to obtain jellyfish antihypertensive peptide powder. Background technique [0002] Jellyfish, also known as jellyfish, is a marine coelomate. It not only has many types and large numbers, but also has a wide distribution. It is an important part of marine zooplankton communities. Jellyfish is extremely nutritious, and its body contains high protein and inorganic salts such as calcium, phosphorus, iodine, iron, etc., and is known as the "treasure of aquatic products". Jellyfish is not only edible, but also a good medicine for curing diseases blindly. According to "Compendium of Materia Medica": jellyfish has the functions of clearing away heat and detoxification, reducing phlegm and s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 张绵松刘昌衡唐聚德孟秀梅刘新张永刚袁文鹏王小军孙永军胡炜贾爱荣夏雪奎
Owner SHANDONG HOMEY AQUATIC DEV
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