128 results about "Blood coagulation time" patented technology

A device for measuring blood coagulation time is formed from a first substrate; a second substrate; a spacer layer disposed between the first and second substrates, said spacer layer having an opening formed therein defining a sample receiving chamber, a vented sink chamber, and an elongated reservoir forming a conduit for liquid movement between the sample receiving chamber and the sink chamber; a first electrode disposed on the first substrate, said first electrode being exposed in the reservoir portion through a first opening in the spacer layer; and a second electrode disposed on the second substrate, said second electrode being exposed in the reservoir portion through a second opening in the spacer layer. The device of the invention is used in combination with an apparatus that is connected to the first and second electrodes for measuring current flow between the first and second electrodes. Changes in observed current are indicative of flow through the device, and a cessation of flow indicates coagulation.

The present inventors produced a variety of bispecific antibodies that specifically bind to both F. IX / F. IXa and F. X, and functionally substitute for F. VIIIa, i.e., have a cofactor function to promote F. X activation via F. IXa. Among these antibodies, the antibody A44 / B26 reduced coagulation time by 50 seconds or more as compared to that observed when the antibody was not added. The present inventors produced a commonly shared L chain antibody from this antibody using L chains of A44, and showed that A44L can be used as commonly shared L chains, although the activity of the resulting antibody is reduced compared to the original antibody (A44HL-B26HL). Further, with appropriate CDR shuffling, the present inventors successfully produced highly active multispecific antibodies that functionally substitute for coagulation factor VIII.

A high-throughput automatic analyzer integrates a biochemical analysis section and a blood coagulation analysis section. The analyzer is capable of achieving a reduction in size, system cost, and lifecycle cost. The automatic analyzer includes: a reaction disk; a first reagent dispensing mechanism that dispenses a reagent to reaction cells on the reaction disk; a photometer that irradiates a reaction solution in the reaction cell with light; a reaction cell cleaning mechanism; a reaction vessel supply unit that supplies a disposable reaction vessel for mixing and reacting a sample and a reagent with each other; a second reagent dispensing mechanism that dispenses a reagent to the disposable reaction vessel; a blood coagulation time measuring section that irradiates a reaction solution in the disposable reaction vessel with light to detect transmitted or scattered light; and a sample dispensing mechanism that dispenses a sample to the reaction cell and the disposable reaction vessel.

In order to improve accuracy of determination of a blood coagulation time without requiring complicated work, a measurement unit 2 of a blood coagulation analyzer 1 irradiates a measurement specimen prepared by mixing a blood specimen and a reagent together, with lights of a plurality of wavelengths including light of a wavelength λ1 and light of a wavelength λ2, obtains information regarding an amount of transmitted light that transmits through the measurement specimen, and transmits the obtained information to a control device 4. The control device 4 calculates a blood coagulation time of the blood specimen based on information regarding a transmitted light amount based on light of the wavelength λ1. Moreover, the control device 4 determines whether a blood coagulation time can be appropriately obtained through the measurement, based on information regarding transmitted light amounts based on light of wavelength λ1 and light of wavelength λ2.

A blood coagulation analyzer and analyzing method perform following: (a) preparing a measurement specimen by dispensing a blood specimen and a reagent into a reaction container; (b) emitting light of a plurality of wavelengths to the measurement specimen in the reaction container, the wavelengths comprising a first wavelength for use in a measurement by a blood coagulation time method, and at least one of a second wavelength for use in a measurement by a synthetic substrate method and a third wavelength for use in a measurement by an immunoturbidimetric method; (c) detecting light of a plurality of wavelengths corresponding to the light emitted in (b), from the measurement specimen, by a light receiving element, and acquiring data corresponding to each wavelength; and (d) conducting an analysis based on the data corresponding to one of the wavelengths among the acquired data, and acquiring a result of the analysis.

The invention discloses a blood coagulation time measuring device, which comprises a light source, an optical receiver element, a test glass in which a detected sample is placed and a steel ball in the test glass, the test glass is arranged between the light source and the optical receiver element, and the three parts are all positioned on the same axial line, the steel ball can be vibrated to and fro in the detected sample in the test glass along the direction vertical to the axial line, and measuring light sent by the light source reaches the optical receiver element after passing through the detected sample and the steel ball in the test glass; and the invention also discloses two methods of an optical method and a mechanical method for measurement by applying the measuring device. Thedevice and methods have the advantages of simple structure, low costs, realizable two test functions of the optical method and the mechanical method.

In order to improve accuracy of determination of a blood coagulation time without requiring complicated work, a measurement unit 2 of a blood coagulation analyzer 1 irradiates a measurement specimen prepared by mixing a blood specimen and a reagent together, with lights of a plurality of wavelengths including light of a wavelength »1 and light of a wavelength »2, obtains information regarding an amount of transmitted light that transmits through the measurement specimen, and transmits the obtained information to a control device 4. The control device 4 calculates a blood coagulation time of the blood specimen based on information regarding a transmitted light amount based on light of the wavelength »1. Moreover, the control device 4 determines whether a blood coagulation time can be appropriately obtained through the measurement, based on information regarding transmitted light amounts based on light of wavelength »1 and light of wavelength »2.

The invention relates to a blood coagulation time test and analysis device which is characterized by comprising a test piece (22) and a portable main machine, wherein a sample hole (1) for containing a blood sample, a reagent hole (2) for containing a reagent and a capillary tube (3) with a conducting layer (5) are formed in the test piece (22); the sample hole (1), the reagent hole (2) and the capillary tube (3) are communicated with one another in sequence; an air hole (4) is formed in the tail end of the test piece (22), so that liquid can flow in the capillary tube (3); the conducting layer (5) is connected with an electrode interface (7); the electrode interface (7) is accessed into the portable main machine; when the blood sample is mixed with the reagent, the mixed liquid flows along the capillary tube (3); along with the flowing of the mixed liquid, the resistance of the conducting layer (5) changes; the portable main machine is used for measuring the time required by blood coagulation through obtaining a resistance change stop time point.

The invention discloses a piezoelectric thin-film resonator for blood coagulation detection. The piezoelectric thin-film resonator comprises a piezoelectric film layer and an annular groove. In addition, the invention also discloses a method for manufacturing the piezoelectric thin-film resonator for blood coagulation detection. The method includes: the inner surface of an annular groove is coated with a hydrophilic polymer material; oxygen plasma treatment or ultraviolet light irradiation is carried out on the inner surface of the annular groove; a piezoelectric thin-film resonator is placed in a sodium carbonate solution and a calcium chloride solution is added into the solution; and the piezoelectric thin-film resonator is baked until the piezoelectric thin-film resonator is dried. Besides, the invention also discloses a method for blood coagulation detection by using a piezoelectric thin-film resonator. The method for blood coagulation detection comprises: dripping mixed liquid having a blood sample and a blood coagulation testing regent into the center of an annular groove; measuring the resonant frequency of a piezoelectric thin film resonator; and according to the recorded stabilized resonant frequency, calculating a second derivative of the resonant frequency to a time change and taking a maximum value of the second derivative as blood coagulation time. The method for blood coagulation detection by using a piezoelectric thin-film resonator is suitable for household application.

The invention provides compound micro-porous cross-linked starch styptic powder which is prepared by emulsification and cross-linking of one of purified water, potato starch, sodium alginate and sodium carboxymethyl cellulose, an emulsifying agent, a cross-linking agent and a dispersing agent as raw materials, wherein the emulsifying agent is selected from Span and Tween; the cross-linking agent is selected from glutaric dialdehyde, sodium trimetaphosphate, formaldehyde and epoxy chloropropane; and the dispersing agent is selected from paraffin and vegetable oil. The compound micro-porous cross-linked starch styptic powder has the following technical effects: micro-porous starch styptic materials are spherical particles extracted from plant starch and subjected to micro-porous treatment; when micro-porous polysaccharide styptic particles are put on a bleeding wound, the molecules of the particles absorb moisture in blood, and visible components in the blood are aggregated on the surfaces of the particles so as to form a gelatinous mixture, thereby achieving the effect of stopping bleeding in time; and meanwhile, activation of endogenous blood coagulation factors is accelerated, the blood coagulation time is shortened, a local clot is formed, and the styptic effect is finished within tens of seconds.

The present invention relates to an RFID-based bio sensor measurement device and a measurement method using same allowing element measurement in conjunction with a wireless communication device such as a smart phone. The NFC or RFID-based bio sensor measurement device according to the present invention includes a bio sensor portion (100) where a reaction reagent is embedded; and an NFC or RFID portion (200) that is electrically connected to the bio sensor portion (100), wirelessly receives electric power from an external electronic device, and measures a measurement target value by using the bio sensor portion (100) and then wirelessly transmits the result to the external electronic device. With the NFC or RFID-based bio sensor measurement device according to the present invention, no additional measuring device has to be purchased and strips can be purchased one by one for economic advantages. Also, the present invention uses a portable smart device which is a personal necessity of modern people, and thus blood sugar, anemia, blood coagulation time, and the like can be diagnosed on the site even in the absence of a measuring system during a trip or the like.

The invention discloses an automatic artery compression haemostat and a hemostasis method. The haemostat comprises a tourniquet. An airbag is arranged on the tourniquet and is connected with an air pump, a deflation valve and a pressure sensor. A pre-amplification device is connected with the output end of the pressure sensor. An output signal of the pre-amplification device is divided into two channels, one channel is directly sent to a port AD0 of the AD (analog to digital) conversion end of a single microcomputer chip, and the other channel is connected with a port AD1 of the AD conversion end of the single microcomputer chip through a pulse signal processing circuit. The single microcomputer chip controls movements of the air pump and the deflation valve through connection. The artery hemostasis with pressure measuring and timing functions is used for hemostasis, pressure as needed by compression of artery puncture points is set according to individual blood pressure values of patients, and compression time as needed by radial artery puncture points is set according to bleeding and blood coagulation time of the patients and half-life periods of anticoagulants used in operations, so that hemostasis effect of the artery puncture points is improved, comfort level of the patients is also improved, complications caused by ultrahigh or ultralow pressure are avoided, and working efficiency of medical workers is improved.

A device for measuring blood coagulation time is formed from a first substrate; a second substrate; a spacer layer disposed between the first and second substrates, said spacer layer having an opening formed therein defining a sample receiving chamber, a vented sink chamber, and an elongated reservoir forming a conduit for liquid movement between the sample receiving chamber and the sink chamber; a first electrode disposed on the first substrate, said first electrode being exposed in the reservoir portion through a first opening in the spacer layer; and a second electrode disposed on the second substrate, said second electrode being exposed in the reservoir portion through a second opening in the spacer layer. The device of the invention is used in combination with an apparatus that is connected to the first and second electrodes for measuring current flow between the first and second electrodes. Changes in observed current are indicative of flow through the device, and a cessation of flow indicates coagulation.

Provided is a method of measuring blood coagulation time which makes it possible to detect lupus anticoagulants more easily and with a higher degree of sensitivity in comparison to the method recommended by the International Society on Thrombosis and Haemostasis (ISTH), and which is not affected by blood-coagulation-factor deficiencies, even in blood samples from patients on warfarin, patients with vitamin K deficiencies and patients with hepatic insufficiency. The method for measuring blood coagulation time to detect lupus anticoagulants is characterized in that a buffering solution composition containing blood coagulation factors is added to a blood sample both before and during the measurement of the blood coagulation time, and the blood coagulation time is measured.

A signal reference value (SLocal) is set at which a blood coagulation reaction time (T) of a blood coagulation time reference sample measured on the basis of the result of comparing a signal value (amount of transmitted light, amount of scattered light, amount of fluorescence, or turbidity) pertaining to blood coagulation time that varies temporally according to the mixing and reaction of the blood coagulation time reference sample and a reagent and a signal reference value (S) corresponds to an expected value (Te) for the blood coagulation reaction time that has been set beforehand so as to correspond to the blood coagulation time reference sample. As a result, it is possible to use the blood coagulation time reference sample to determine the state of the reagent and enhance the reliability of measurement results by setting a unique signal reference value for each reagent container.

The invention relates to the technical field of medical materials and particularly relates to rapid hemostatic powder for an injury and a preparation method of the rapid hemostatic powder. The rapid hemostatic powder for the injury comprises the following raw materials in parts by weight: 40-56 parts of chitosan quaternary ammonium salt, 28-36 parts of puffball extracts, 8-12 parts of sodium alginate, 1-5 parts of ramie root extracts, 1-5 parts of cirsium setosum extracts, 1-5 parts of madder extracts and 0.5-1.5 parts of carbonized hair extracts. The rapid hemostatic powder for the injury can take a hemostasis effect through various approaches and remarkably shorten the blood coagulation time, also has the effects of inhibiting and resisting bacteria, relieving pain and promoting wound healing and can be widely applied to rapid hemostasis, emergency treatment and treatment of skin scratch, injury and haemorrhoids and relief of scar formation.

Provided is a blood coagulation analyzer whereby both of an adequate dynamic range and a high sensitivity can be achieved in analyzing blood coagulation, without using any complicated device, by selecting an appropriate detection angle depending on the scattering light intensity of each specimen. The blood coagulation analyzer comprises: a reactor (101) in which a sample and a reagent are mixed together and reacted; a light source (102) for irradiating the reactor (101) with light; multiple detectors (103a, 103b), which are positioned around the reactor (101) at different angles to the light axis of the light from the light source (102), for detecting scattering light generated from the liquid mixture of the sample and the reagent; a memory unit (107) for inputting and memorizing multiple light intensity change data over time obtained from the detectors (103a, 103b); a determination unit (106) for selecting, depending on the amount of light intensity change, light intensity change data to be used in calculating blood coagulation time from among various light intensity change data stored in the memory unit (107); and a calculation unit (108) for calculating the blood coagulation time based on the light intensity change data selected by the determination unit (106).

The invention discloses a scopoletin derivative as well as a preparation method and an application thereof. The general formula of the scopoletin derivative is formula (I), wherein R is selected from C1-4 alkyl and X is selected from a halo substituent. The scopoletin derivative has a remarkable anticoagulation effect and can remarkably prolong the in vitro blood coagulation time. The scopoletin derivative orally taken can quickly enter into blood to have the anticoagulation effect. The onset time and the curative effect of the scopoletin derivative for treating venous thromboembolic diseases are equivalent to those of warfarin and the scopoletin derivative is smaller in dosage and easy to control. The scopoletin derivative has novel medical use of preventing and treating venous thromboembolic diseases and meanwhile provides a novel treatment candidate medicine for patients with venous thromboembolic diseases. The invention further discloses the preparation method of the scopoletin derivative and the application of the scopoletin derivative in preparing anticoagulation medicines.

A recombinant thrombin-like enzyme batroxobin expressed by yeast, a production method thereof, and use of the batroxobin as a hemostatic agent or antithrombotic agent is disclosed. In order to express recombinant thrombin-like enzyme, an expression vector is prepared by inserting cDNA encoding the enzyme, the transformed cell is prepared by introducing the expression vector into the cell, the transformed cell then is incubated and the recombinant thrombin-like enzyme is obtained from the cell. The recombinant thrombin-like enzyme may be usefully used as a hemostatic agent because the enzyme effectively decrease bleeding time and blood coagulation time and does not affect various blood coagulation factors. The recombinant thrombin-like enzyme is also useful for a hemostatic agent or antithrombotic agent comprising the recombinant thrombin-like enzyme as an active component

Provided is a method of measuring blood coagulation time, the method being capable of LA detection easily and with high sensitivity as compared with the method recommended by the ISTH, without being affected by deficiency of blood coagulation factors even in a blood sample of a warfarin taker, a person who suffers from vitamin K deficiency, or a hepatic failure patient. Disclosed is a method of measuring the blood coagulation time to detect lupus anticoagulant, the method including adding a buffer solution composition containing blood coagulation factors to a blood sample before measurement or at the time of measurement of the blood coagulation time, and measuring the blood coagulation time.

The invention discloses an improved synthesis method for dencichine, and belongs to the fields of medicine and chemical engineering. Dencichine is a white solid, and can be used for hemostasis by shortening blood coagulation time and thrombinogen time, thereby being important medicine; while, natural dencichine is low in extraction rate, and low in profit margin. The improved synthesis method is an efficient way to obtain dencichine according to a chemosynthesis method, and is low in cost, suitable for industrial production, and low in reaction cost, thereby providing an important reference for industrialization.

The invention discloses an antithrombotic medicine. The antithrombotic medicine is prepared by evenly mixing, by weight, 0.3-99.7 parts of fructus cannabis extract and 99.7-0.3 part of industrial cannabinoid. Experiments show that the medicine has a remarkable antithrombotic effect, can remarkably suppress formation of thrombus in the bodies of rats, has a remarkable suppressing effect on platelet aggregation of rats, can remarkably delay the blood coagulation time of rats, can reduce the whole blood viscosity, plasma viscosity and packed cell volume of rats, has a better acting effect than cannabidiol, and has good application prospects.

The invention discloses a blood coagulation time measuring method, device and system. The blood coagulation time measuring method comprises the steps of in response to a starting instruction, startingblood coagulation detection timekeeping; obtaining a signal value used for indicating a steel ball amplitude; comparing the steel ball amplitude indicated by the signal value with a steel ball amplitude indicated by a preset signal value; recording the lasting time when the steel ball amplitude indicated by the signal value is smaller than the steel ball amplitude indicated by the preset signal value; if the lasting time is larger than or equal to a first preset time, stopping blood coagulation detection timekeeping. The blood coagulation time measuring method can effectively solve the problem that a blood coagulation time measuring result is smaller than an actual result.

The invention belongs to the technical field of biomedical materials, and particularly relates to breviscapine / chitosan composite hydrogel restraining cicatrization and a preparation method thereof. The hydrogel is formed by compositing breviscapine, chitosan, polyvinyl alcohol and soluble starch. Due to the synergistic effect of the breviscapine and the chitosan and the effective combination of the chitosan with the high molecular weight and the chitosan with the low molecular weight, the composite hydrogel can rapidly heal a wound, has the advantages of achieving hemostasis and bacteriostat and restraining cicatrization from the source, and can shorten the blood coagulation time, accelerate wound healing and reduce cicatrization. The preparation method of the hydrogel is simple, rapid, convenient and high in practicability, the adopted high molecular materials are non-toxic and biodegradable materials, and the obtained composite hydrogel has the advantages of being good in biocompatibility, good in biodegradability, good in absorptivity, safe, non-toxic and the like, and has the good application prospect.

The invention discloses a blood coagulation analysis device. The device comprises a detection bin, an inlet and outlet, an image acquisition module and an image processing module. The inlet and outletis at the same level as the detection bin; the image acquisition module is located below or above the detection bin; and the image acquisition module is in communication connection with the image processing module. The detection bin is used for placing a disposable reaction cup; the inlet and outlet is used for achieving horizontal placing or removing the disposable reaction cup; the image acquisition module is used for continuously photographing the disposable reaction cup in the detection bin and transmitting photographs to the image processing module; the image processing module is used for receiving image information collected by the image acquisition module, and processing and analyzing the image information to determine blood coagulation process and blood coagulation time in the disposable reaction cup. Compared with the prior art, the device has the advantages of compact structure, convenient carrying, wide application range, high measurement accuracy and accurate measurement result.

Disclosed is an analysis method for a blood specimen, including: obtaining a data group including a plurality of data forming a blood coagulation curve or a differential curve thereof; inputting the data group into a deep learning algorithm; and outputting, on the basis of a result obtained from the deep learning algorithm, information regarding a cause of prolongation of blood coagulation time of the blood specimen.