34 results about "Batroxobin" patented technology

The present invention relates to a platelet aggregation function detection kit, and a method using the kit to detect platelet aggregation function. The detection kit mainly includes the following detection reagents: a sodium citrate whole blood activator, a fibrin activator and a platelet activator. The fibrin activator comprises: recombinant batroxobin and activated clotting factor. By using the kit of the invention to detect, blood sample processing requirements are low, repeat testing variability is small, accuracy and stability are high, use and storage are convenient, detection cost is low, and promotion is easy.

The present invention provides a new type balloon dilation catheter which includes ballon and medication material coated on stent. Said medication material comes from one or two and more than two mixtures of heparin sodium, fiber degrading enzyme, serine proteinase, batroxobin, aspirin, genistein, hirudin and its recombined product, colchicine, sirolimus, biolimus, zotarolimus, tracrolimus, pimecrolimus, simvastatin, atorvastatin, pravastatin, ciclosporin, Anti-CD34, dexamethasone, bleomycin, plicamycin, daunomycin, mitomycin C, actinomycin D, taxol, celastrol, methopterin, 5-fluorouracil, cytarabine and 6-purinethol. The balloon is made of macromolecule nylon material, and the stimulation to blood vessel is far lower than the stent with metal structure.

The present invention provides a recombined-batroxobin, having following characteristics: (a) the molecular weight is 29-32Kda; (b) at least 90% batroxobin in the recombined-batroxobin has correct 6 pairs of disulfide bonding; Cys7-Cys139, Cys26-Cys42, Cys74-Cys230, Cys118-Cys184, Cys150-Cys163 and Cys174-Cys199; (c) the 146 position and the 225 position in the SEQ ID NO:1 are suffered from glycosylation; (d) the specific activity of the batroxobin is more than 1500KU/mg.

The fibrinogen plate method for detecting thrombase, batroxobin and thrombase inhibitor includes preparing fibrinogen plate with agarose and fibrinogen and quantitative detection via the diffusion reaction of standard sample and detected sample on the plate. The detection method of the present invention has simple operation, high sensitivity, being intuitive and stable, no interference of the turbidity and color of the detected sample and low cost. The detection method may be realized simply in lab with thermotank.

The invention relates to a site-directed mutagenesis genetic engineering batroxobin is capable of clotting animal plasma containing an anti-coagulant agent. The site-directed mutagenesis genetic engineering batroxobin is characterized in that: compared with the amino acid sequence of a natural batroxobin, the amino acid sequence of the site-directed mutagenesis genetic engineering batroxobin has a mutation from Arg to Lys at the 45th digit. The site-directed mutagenesis genetic engineering batroxobin having higher bioactivity can be produced in a large scale through a secreted expression mode.

A recombinant thrombin-like enzyme batroxobin expressed by yeast, a production method thereof, and use of the batroxobin as a hemostatic agent or antithrombotic agent is disclosed. In order to express recombinant thrombin-like enzyme, an expression vector is prepared by inserting cDNA encoding the enzyme, the transformed cell is prepared by introducing the expression vector into the cell, the transformed cell then is incubated and the recombinant thrombin-like enzyme is obtained from the cell. The recombinant thrombin-like enzyme may be usefully used as a hemostatic agent because the enzyme effectively decrease bleeding time and blood coagulation time and does not affect various blood coagulation factors. The recombinant thrombin-like enzyme is also useful for a hemostatic agent or antithrombotic agent comprising the recombinant thrombin-like enzyme as an active component

It' s a method for extracting Batroxobin for defibrination and thrombolysis from venom of snakes, which relates to medicine technologies. The method comprises the steps of soaking, dissolving and centrifuging the venom of the snake; chromatography of the supernatant by an affinity column; chromatography of the eluent that has been desalinated by flowing it through polydextran gel G25; entrapment and concentration of the eluent that containing effective compositions and chromatography of the concentrated sample by a molecular sieve so as to obtain the Batroxobin with sole composition. The method features convenient operation, short production cycle, high purity and is suitable for production in large scale.

The present invention discloses batroxobin and its preparation process and specific coding gene. The batroxobin coding gene possesses one of the following nucleotide sequence: 1. the sequence 2 in the sequence list; 2. the sequence 2 in the sequence list with 1-3 mutant nucleotides; and 3. the nucleotide sequence obtained through connecting IL-2 signal peptide gene sequence to the 5' end of the said sequence. During preparation process of batroxobin, any one coding gene of the nucleotide sequence 1-3 is introduced through eukaryon expression vector into yeast or mammal cell line for expression to obtain batroxobin protein. The process of the present invention expresses batroxobin protein in high bioactivity successfully, and obtains purified yeast yield of 10 mcg/ml or 14 BU/ml and in specific activity of 1400 BU/mg. The present invention has high yield and is suitable for industrial production.

A compound medicine for arresting bleeding is characterized in that the compound medicine is a mixture of a batroxobin and an interleukin-11; the clinical dosage of the batroxobin is 0.01-10u and that of IL-11 is 0.06-50mg; the batroxobin is the batroxobin from snake venom of Brazilian spearhead adder, white-eyebrow adder or acutus adder, or the batroxobin from recombinant Brazilian spearhead adder, white-eyebrow adder or acutus adder obtained by genetic engineering; the interleukin-11 is produced by human bone-marrow stromal cells (fibroblasts) and interstitial cells, or the recombinant IL-11 by genetic engineering. The medicine for arresting bleeding can not only ensure the curative effect but also decrease the amount of protease so as to reduce the rate of adverse immune response.

A preparation for inhibiting local invasion of malignant tumors is provided which comprises batroxobin and therefore can inhibit local invasion of malignant tumors. A preparation for encapsulating malignant tumor tissues is also provided which comprises batroxobin and therefore can cause or promote formation of capsule-like tissue around malignant tumor tissues.

A purified recombinant batroxobin with high specific activity, which has the following properties: (a) the batroxobin has a molecular weight of 29-32 kDa; (b) at least 90% of the batroxobin have 6 pairs of disulfide bonds which correctly match at Cys⁷-Cys¹³⁹, Cys²⁶-Cys⁴², Cys⁷⁴-Cys²³⁰, Cys¹¹⁸-Cys¹⁸⁴, Cys¹⁵⁰-Cys¹⁶³ and Cysl¹⁷⁴-Cys¹⁹⁹; (c) positions 146 and 225 in SEQ ID NO:1 are modified as N-glycosylation; and (d) the specific activity of the batroxobin is equal to or greater than 1500 KU/mg protein.

A directed mutation gene engineering batroxobin and its use are disclosed. The process is carried out by deleting 10 amino acids at C-end of batroxobin amino-acid sequence and mutating No.133 Tyr to Glu. It has better biological activity.

The invention belongs to the field of medicines, and particularly relates to a medicinal composition for treating acute cerebral ischemic stroke. The medicinal composition is prepared from pharmaceutically acceptable medicinal auxiliary materials, batroxobin, citicoline, ginkgolide and protoporphyrin sodium. The dosage form of the medicinal composition is preferably lyophilized powder for injection. The protoporphyrin sodium in the medicinal composition has a significant effect of enhancing the batroxobin functions of preventing thrombosis and improving cerebral blood perfusion, so that improvement on the cerebral ischemic stroke treating effect of the batroxobin is benefited.

The invention belongs to the field of traditional Chinese medicine, and aims to provide a method for fermenting traditional Chinese medicine with batroxobin yeast. The method can effectively improve a medicinal material use rate and enhance the medicinal effect. For realizing the purposes, an adopted technical solution comprises: placing prepared medicinal materials for external application into a container, adding water for cooking for 3-5h, then naturally cooling to 28-36 DEG C, adding batroxobin and yeast, and preserving temperature and fermenting for 1-3h, wherein a ratio of the batroxobin to the medicinal materials is 500-1000BU to 100g, a ratio of the yeast to the medicinal materials is 2-3g to 100g. The batroxobin can effectively dissolve fibers in the traditional Chinese herbal medicines, cause effective components wrapped in the fibers in the medicinal materials to be released, improve the effective medicinal material use rate and enhance the medicinal effect. The batroxobin has the effects of reducing blood viscosity, dissolving fibrinogen, inhibiting thrombogenesis and dissolving thrombus, and can promote blood of a human body to flow and improve the absorbing ability of the human body to the medicine.

The batroxobin is a hemostatic which is widely used at present. The invention relates to production of recombinant batroxobin by continuous fermentation induction of a mixed carbon source of methanol and glucose. And the recombinant batroxobin in a fermentation supernatant is purified by adopting an ionic membrane filtration and high-recovery-rate chromatography method.

The present invention relates to a novel recombinant batroxobin mixture, a hemostatic composition comprising the same, and a method for preparing the same. According to the present invention, the hemostatic composition of the present invention has an excellent hemostatic effect by suppressing rebleeding, and maintains activity thereof even when prepared in a solid form, and thus, the composition of the present invention can be easily used by being applied as a topical hemostatic agent.

The invention discloses a progressive stroke retardant which is characterized in that the dosage per day comprises the following components of: 100-300mg of bayaspirin, 75-300mg of clopidogrel, 10-20mg of Lipitor, 5-20BU of batroxobin and 15-45 Danshen dropping pills. According to the provided progressive stroke retardant, multiple targets act on multiple progressive parts of stroke, and the progressive stroke retardant has obvious effects of stabilizing, preventing and breaking inflammatory response and pathogenic plaques of offending vessels, reducing the blood fibrin concentration, dilating blood vessels, improving collateral circulation and resisting platelet aggregation and has obvious effects on progressive diseases, disease outcome and prognosis of progressive stroke patients.