4results about How to "Enhance electrostatic interaction" patented technology

This invention discloses a method for applying hydrothermally loaded carbon oxalate ore to heavy metal adsorption. This method is the first to discover the role of hydrothermally loaded carbon oxalate ore in the adsorption of arsenic and cadmium, and particularly reveals its synergistic adsorption effect on arsenic and cadmium. Specifically, the presence of arsenic promotes the adsorption of cadmium. This invention avoids competitive adsorption between cadmium and arsenic, exhibiting high adsorption capacity for both, providing a new approach for the rapid and efficient simultaneous treatment of arsenic and cadmium in water.

The invention discloses a thermal response type environment-friendly anti-collapse agent and a preparation method thereof, and relates to the technical field of oilfield chemistry. The method comprises the following steps: taking carboxylated thermal expansion graphite, benzene ring-containing amino acid and cationic amino acid, uniformly mixing, carrying out high-temperature melt polycondensation reaction under the action of alkali, adding long-chain fatty amine after the reaction, continuing the reaction, and cooling and crushing the reaction product. The anti-collapse agent disclosed by the invention is extremely weak in adhesion effect at normal temperature and does not play a role, and can be gradually softened in a high-temperature environment, so that the adhesion performance is remarkably enhanced, and the anti-collapse agent has the characteristic of temperature response; when being used as an anti-collapse agent, the anti-collapse agent has the advantages of good adhesiveness, strong wall fixing property, good compatibility, high temperature resistance and the like.

This invention discloses an engineered mutant CpAgo_PM based on a novel PAZ nucleic acid binding pocket and its applications, belonging to the fields of molecular biology and genetic engineering. The amino acid sequence of the mutant CpAgo_PM is shown in SEQ ID NO.1. This invention rationally designs the PAZ nucleic acid pocket, mutating some normally non-positively charged amino acid residues in the pocket to positively charged amino acids (CpAgo_PM). In in vitro cleavage experiments, four different target sites (TET2-1 / 2 / 3 / 4) on the human TET2 gene transcript were tested. Compared with CpAgo_WT, CpAgo_PM significantly improves the cleavage efficiency at three sites: TET2-1, TET2-2, and TET2-4, indicating that it partially overcomes the sequence cleavage bias of the wild type. Furthermore, its gene editing function in a eukaryotic cell environment has been clearly demonstrated.

The application belongs to the field of engineering technology and provides a preparation method of a nucleic acid separation device. The preparation method of the application prepares a hydrogel nucleic acid adsorption device through a photo-initiated radical polymerization reaction, avoiding the cumbersome limitations of traditional polymerization reactions which need to be carried out under anaerobic conditions. The preparation method is simple and fast, and does not depend on professional and precise instruments. The raw materials are cheap and easy to obtain. The method can efficiently load silica particles, effectively combine the plasticity of the hydrogel with the high specific surface area of the silica particles and the strong binding capacity of the nucleic acid, and further obtain a handheld nucleic acid extraction device which can change shape, size and quantity at any time according to application requirements, has a high specific surface area and excellent nucleic acid extraction performance. Compared with traditional processes, the preparation process is simpler, the application range is wider, and the application requirements of low resource environment are greatly met, and the application prospect in the field of outdoor and clinical nucleic acid extraction technology is broad.