Anti-aging composition for increasing corium layer collagen and preparation method of anti-aging composition

Abstract

The invention relates to the technical field of compositions, in particular to an anti-aging composition for increasing corium layer collagen and a preparation method of the anti-aging composition. Comprising the following steps: preparing sargassum fusiforme polysaccharide; preparing a composite sargassum fusiforme polysaccharide stabilizer; and compounding and preparing the traditional Chinese medicine extract. The anti-aging composition is prepared by compounding the balm extract, the pistacia chinensis extract, the mulberry extract, the collagen tripeptide and the sargassum fusiforme polysaccharide, all the components are properly compounded, the obtained anti-aging composition and oral liquid are not prone to precipitation in the storage process, loss of nutritional ingredients is avoided, and the anti-aging effect is good. According to the present invention, the components have the obvious synergistic effect, such that the collagen secretion can be promoted while the oxidative stress, the collagen degradation and the skin inflammation can be inhibited so as to substantially increase the collagen content of the skin corium layer, and the anti-aging effect on the skin can be effectively achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of compositions, and in particular to an anti-aging composition for increasing dermal collagen and a preparation method thereof. Background Art

[0002] As human demand for health and beauty continues to increase, anti-aging products have gradually become a hot area of ​​research and development. Skin aging is one of the most significant characteristics of human aging, which is mainly manifested in reduced collagen content in the dermis, decreased skin elasticity, and increased wrinkles. These phenomena are mainly due to increased free radicals or lack of antioxidants, which increase cytotoxicity and reduce the body's antioxidant capacity, inducing body aging and causing dry skin, loss of elasticity and wrinkles. Therefore, how to inhibit oxidative stress and inflammatory responses while promoting the secretion and synthesis of collagen has become an important direction for the development of anti-aging products.

[0003] At present, there are several main ways to fight against aging on the skin: subcutaneous injection, use of anti-aging skin care products, and oral administration of pharmaceutical compositions rich in anti-aging ingredients. Subcutaneous injection has the advantage of rapid effect, but there is a risk of skin rejection and allergies. Invasive operations may also cause local inflammation or infection. The active ingredients of anti-aging skin care products are difficult to penetrate the skin barrier and penetrate into the dermis. They mainly provide moisturizing effects. Oral pharmaceutical compositions rich in anti-aging ingredients are divided into solid drugs and oral liquids. Solid drugs are slowly digested and absorbed after entering the body, and are not suitable for people with poor gastrointestinal function. Oral liquids can fully dissolve the active ingredients in the solvent and can be fully absorbed by the human body. However, the active ingredients in the oral liquid are prone to combine with each other during storage, precipitate and degrade, resulting in insufficient nutrient utilization. Summary of the invention

[0004] In order to solve the above technical defects, the present invention has developed a method for preparing an anti-aging composition for increasing collagen in the dermis, and the anti-aging components are reasonably compounded to form an effective synergistic effect between the components. The prepared anti-aging composition is simple to operate when used in oral liquid, and has good stability, so that the components can be fully utilized, while inhibiting oxidative stress, collagen degradation and skin inflammation, it promotes collagen secretion, and effectively achieves the anti-aging effect on the skin.

[0005] A method for preparing an anti-aging composition for increasing dermal collagen comprises the following steps: S1: Preparation of Sargassum fusiformis polysaccharide The method comprises the following steps: desalting, freeze-drying and grinding the Sargassum fusiformis into powder, extracting the powder with ethanol, dispersing the powder with deionized water and adjusting the pH for enzymatic hydrolysis, adding sodium citrate during the enzymatic hydrolysis to obtain enzymatically hydrolyzed Sargassum fusiformis, adding calcium chloride for precipitation, performing low-temperature alcohol precipitation and freeze-drying to obtain Sargassum fusiformis polysaccharide; S2: Preparation of composite Sargassum polysaccharide stabilizer Dissolving Sargassum fusiformis polysaccharide in distilled water at low temperature overnight, and then subjecting it to electric treatment in a pulse current to obtain an electric-treated Sargassum fusiformis polysaccharide solution, dissolving collagen tripeptide in distilled water at low temperature overnight to obtain a collagen tripeptide solution, injecting the collagen tripeptide solution into the electric-treated Sargassum fusiformis polysaccharide solution, and then subjecting it to magnetic stirring to obtain a composite Sargassum fusiformis polysaccharide stabilizer; S3: Compound preparation of Chinese herbal medicine extracts The reddened Pistacia flowers are picked, the corrupt and old parts are removed, and then they are dried and crushed. Then, ultrasonic extraction is carried out in ethanol. After drying, they are dispersed with pure water, and then organic solvent extraction is carried out. The water layer is retained and dried to obtain the Pistacia flower extract. The lemon balm extract, the Pistacia flower extract and the mulberry extract are added to the composite Sargassum fusiformis polysaccharide stabilizer and stirred evenly. After sterilization, the anti-aging composition is canned and sealed.

[0006] Furthermore, step S1 of preparing Sargassum fusiformis polysaccharide comprises the following steps: S1.1: 1-1.5 parts of Sargassum fusiformis are washed and desalted, freeze-dried, ground into powder, and extracted with an ethanol solution having a concentration of 92-95%, the extraction is repeated three times, and then dried to obtain pretreated Sargassum fusiformis powder, the pretreated Sargassum fusiformis powder is dispersed with 10-12 parts of deionized water, the pH is adjusted to 4-4.5, 0.5-0.6 wt % of cellulase and 0.1-0.2 wt % of sodium citrate are added, the mixture is shaken and extracted at 50-55° C. for 18-20 hours, and then boiled in hot water at 95-98° C. for 12-15 minutes to obtain enzymolyzed Sargassum fusiformis; S1.2: Add 0.02-0.03 parts of calcium chloride to the enzymolyzed Sargassum obtained in step S2.1, stir and precipitate, centrifuge and separate after no precipitate is generated, collect the supernatant, adjust the pH to neutral, and then concentrate to 1 / 4-1 / 3 of the original volume, add 4-5 times the volume of ethanol, and let stand at 3-4°C for 8-10 hours. Collect the precipitate by centrifugation and freeze-dry it to obtain Sargassum polysaccharide.

[0007] Further, step S2 prepares the composite Sargassum fusiformis polysaccharide stabilizer, comprising the following steps: S2.1: Stir the Sargassum fusiformis polysaccharide in distilled water at 65-70°C until it is completely dissolved, and keep it at 3-4°C overnight to prepare a Sargassum fusiformis polysaccharide aqueous solution with a concentration of 6.5-8%, take 8-10 portions of the Sargassum fusiformis polysaccharide aqueous solution and place it in a rectangular container equipped with parallel electrodes, the parallel electrodes are spaced 7.5-9 cm apart, and both ends of the electrodes are connected to the positive and negative poles of an AC power supply, so that the Sargassum fusiformis polysaccharide aqueous solution immerses the two electrodes and then powers on, first at a voltage of 4-6V for 3-4 minutes, then at a voltage of 8-10V for 4-5 minutes, the voltage is selected in a pulse mode, the power is on for 10-15 seconds, and then the power is turned off for 10-15 seconds, to obtain an electrically treated Sargassum fusiformis polysaccharide solution; S2.2: Place 1.2-1.6 parts of collagen tripeptide in 8-10 parts of distilled water at 40-45°C, stir at a stirring speed of 800-1000rpm for 1-1.5 hours, and keep at 3-4°C overnight to obtain a collagen tripeptide solution. Add the collagen tripeptide solution into a syringe and inject it into the electro-treated Sargassum fusiformis polysaccharide solution obtained in step S2.1 at a temperature of 60-65°C, and then magnetically stir at a stirring speed of 1000-1200rpm for 10-15 minutes to obtain a composite Sargassum fusiformis polysaccharide stabilizer.

[0008] Furthermore, the compound preparation of the Chinese medicine extract in step S3 comprises the following steps: S3.1: Pick the reddish Pistacia chinensis flowers, select and remove the corrupt and old parts, then dry them in a clean and dust-free environment, crush them and pass them through an 80-90 mesh sieve to obtain Pistacia chinensis flower powder, put the Pistacia chinensis flower powder in a container, add 70-75% ethanol solution at a solid-liquid ratio of 1: (18-20), heat it to 55-60°C, and then ultrasonicate it at a power of 250-300W for 40-50 minutes, and obtain an extract after drying, disperse the extract with 15-20 times the volume of pure water, and then add an equal volume of organic solvent for extraction, filter to remove the precipitate, retain the water layer and dry it to obtain Pistacia chinensis flower extract; S3.2: Mix 0.6-1.2 parts of lemon balm extract, 1.2-1.6 parts of Pistacia flower extract and 0.4-0.8 parts of mulberry extract, and then add them to the composite Sargassum fusiformis polysaccharide stabilizer prepared in step S2.2, add 0.4-0.5wt% of phenoxyethanol, stir at a stirring speed of 1000-1200rpm for 20-30 minutes, then sterilize by ultra-high temperature instantaneous sterilization technology, and store in sealed cans to obtain an anti-aging composition.

[0009] Furthermore, the diameter of the syringe needle in step S2.2 is 0.6-0.8 mm.

[0010] Furthermore, the organic solvent in step S3.1 is one of ethyl acetate, petroleum ether or n-butanol.

[0011] An anti-aging composition for increasing collagen in the dermis is prepared by the above-mentioned method for preparing an anti-aging composition for increasing collagen in the dermis.

[0012] The beneficial effects are: 1. The present invention obtains an anti-aging composition by compounding lemon balm extract, Pistacia flower extract, mulberry extract, collagen tripeptide and Sargassum fusiformis polysaccharide. The components are properly compounded. When used in an oral liquid, it only needs to add appropriate flavoring agents and stir evenly. The method of use is simple. The obtained anti-aging composition and oral liquid are not only not easy to precipitate during the storage process, avoiding the loss of nutrients, but also have obvious synergistic effects between the components, so that while inhibiting oxidative stress, collagen degradation and skin inflammation, it can promote collagen secretion, greatly increase the collagen content in the dermis of the skin, and effectively achieve the anti-aging effect on the skin.

[0013] 2. The present invention prepares Sargassum fusiformis polysaccharide into an aqueous solution of Sargassum fusiformis polysaccharide with a concentration of 6.5-8%, and then places it in a container equipped with parallel electrodes, sets a distance between the parallel electrodes, connects the positive and negative electrodes of an AC power supply at both ends of the electrodes, and immerses the two electrodes in the aqueous solution of Sargassum fusiformis polysaccharide, and then powers on, first at a voltage of 4-6V, and then at a voltage of 8-10V, the voltage selects a pulse mode, powers on for 10-15 seconds, and then powers off for 10-15 seconds, thereby increasing the charge excitation degree on the surface of Sargassum fusiformis polysaccharide, and when the collagen tripeptide solution is subsequently injected, the electrostatic interaction between Sargassum fusiformis polysaccharide and protein is enhanced, thereby neutralizing the surface charge of the protein, reducing the aggregation tendency between the proteins, effectively reducing the occurrence of protein precipitation, and improving the stability of the system, so as to ensure that the effective ingredients of the anti-aging composition prepared later are not easily precipitated and degraded when it is made into an oral liquid, thereby avoiding insufficient nutrient utilization.

[0014] 3. The present invention washes and desalts the Sargassum fusiformis, freeze-dries it, grinds it into powder, extracts it with an ethanol solution, repeats this process three times, and then dries it to obtain pretreated Sargassum fusiformis powder. The pretreated Sargassum fusiformis powder is dispersed with deionized water, the pH is adjusted, cellulase and sodium citrate are added, and the powder is extracted at high temperature by shaking. The powder is then boiled in hot water at 95-98°C for 12-15 minutes to obtain the pretreated Sargassum fusiformis powder. Adding sodium citrate during the enzymatic hydrolysis of the pretreated Sargassum fusiformis powder can not only capture metal ions (calcium ions, magnesium ions) that inhibit enzyme activity in the enzymatic hydrolysis system, thereby protecting enzyme activity, but also enhance the ionic strength in the enzymatic hydrolysis system, thereby reducing the aggregation phenomenon between Sargassum fusiformis polysaccharides, thereby improving the enzymatic hydrolysis efficiency and product yield. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1This is a flow chart of the method for preparing the anti-aging composition for increasing dermal collagen used in an embodiment of the present invention. DETAILED DESCRIPTION

[0016] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0017] Example 1: An anti-aging composition for increasing dermal collagen and a preparation method thereof, such as Figure 1 As shown, the following steps are included: S1: Preparation of Sargassum fusiformis polysaccharide S1.1: Wash 1 portion of Sargassum fusiformis with water to remove salt, freeze-dry, grind into powder and extract with 92% ethanol solution, repeat three times and dry to obtain pretreated Sargassum fusiformis powder, disperse the pretreated Sargassum fusiformis powder with 10 portions of deionized water, adjust the pH to 4, add 0.5wt% of cellulase and 0.1wt% of sodium citrate, extract at 50°C with shaking for 18 hours, and boil in hot water at 95°C for 12 minutes to obtain enzymatically hydrolyzed Sargassum fusiformis; S1.2: Add 0.02 parts of calcium chloride to the enzymatically hydrolyzed Sargassum obtained in step S2.1, stir and precipitate, centrifuge and separate after no precipitate is generated, collect the supernatant, adjust the pH to neutral, and then concentrate to 1 / 4 of the original volume, add 4 times the volume of ethanol, and let stand at 3°C ​​for 8 hours. Collect the precipitate by centrifugation and freeze-dry it to obtain Sargassum polysaccharide.

[0018] S2: Preparation of composite Sargassum polysaccharide stabilizer S2.1: Stir the Sargassum fusiformis polysaccharide in distilled water at 65°C until it is completely dissolved, and keep it at 3°C ​​overnight to prepare a 6.5% Sargassum fusiformis polysaccharide aqueous solution, take 8 portions of the Sargassum fusiformis polysaccharide aqueous solution and place them in a rectangular container equipped with parallel electrodes, the parallel electrodes are spaced 7.5 cm apart, and both ends of the electrodes are connected to the positive and negative poles of an AC power supply, so that the Sargassum fusiformis polysaccharide aqueous solution immerses the two electrodes and then powers on, first at a voltage of 4V for 3 minutes, then at a voltage of 8V for 4 minutes, the voltage is selected in a pulse mode, the power is on for 10 seconds, and then the power is turned off for 10 seconds, to obtain an electrically treated Sargassum fusiformis polysaccharide solution; S2.2: Place 1.2 parts of collagen tripeptide in 8 parts of 40°C distilled water, stir at a stirring speed of 800 rpm for 1 hour, and keep at 3°C ​​overnight to obtain a collagen tripeptide solution. Add the collagen tripeptide solution into a syringe with a needle diameter of 0.6 mm, and inject it into the electro-treated Sargassum fusiformis polysaccharide solution obtained in step S2.1 at a temperature of 60°C. Then, perform magnetic stirring at a stirring speed of 1000 rpm for 10 minutes to obtain a composite Sargassum fusiformis polysaccharide stabilizer.

[0019] S3: Compound preparation of Chinese herbal medicine extracts S3.1: Pick the reddish Pistacia chinensis flowers, select and remove the corrupt and old parts, then dry them in a clean and dust-free environment, crush them and pass them through an 80-mesh sieve to obtain Pistacia chinensis flower powder, put the Pistacia chinensis flower powder in a container, add 70% ethanol solution at a solid-liquid ratio of 1:18, heat it to 55°C, and then ultrasonicate it at a power of 250W for 40 minutes, and obtain an extract after drying, disperse the extract with 15 times the volume of pure water, and then add an equal volume of petroleum ether for extraction, filter and remove the precipitate, retain the water layer and dry it, and obtain Pistacia chinensis flower extract; S3.2: Mix 0.6 parts of lemon balm extract, 1.2 parts of Pistacia flower extract and 0.4 parts of mulberry extract, and then add them to the composite Sargassum fusiformis polysaccharide stabilizer prepared in step S2.2, add 0.4wt% of phenoxyethanol, stir at a stirring speed of 1000 rpm for 20 minutes, then sterilize using ultra-high temperature instantaneous sterilization technology, and store in sealed cans to obtain an anti-aging composition.

[0020] Example 2: An anti-aging composition for increasing dermal collagen and a preparation method thereof, such as Figure 1 As shown, the following steps are included: S1: Preparation of Sargassum fusiformis polysaccharide S1.1: 1.5 parts of Sargassum fusiformis were washed and desalted, freeze-dried, ground into powder, and extracted with 92% ethanol solution, repeated three times, and dried to obtain pretreated Sargassum fusiformis powder, the pretreated Sargassum fusiformis powder was dispersed with 12 parts of deionized water, the pH was adjusted to 4, 0.6 wt % of cellulase and 0.2 wt % of sodium citrate were added, and the mixture was shaken and extracted at 50° C. for 18 hours, and then boiled in hot water at 95° C. for 12 minutes to obtain enzymatically hydrolyzed Sargassum fusiformis; S1.2: Add 0.03 parts of calcium chloride to the enzymatically hydrolyzed Sargassum obtained in step S2.1, stir and precipitate, centrifuge and separate after no precipitate is generated, collect the supernatant, adjust the pH to neutral, and then concentrate to 1 / 4 of the original volume, add 4 times the volume of ethanol, and let stand at 3°C ​​for 8 hours. Collect the precipitate by centrifugation and freeze-dry it to obtain Sargassum polysaccharide.

[0021] S2: Preparation of composite Sargassum polysaccharide stabilizer S2.1: Stir the Sargassum fusiformis polysaccharide in distilled water at 65°C until it is completely dissolved, and keep it at 3°C ​​overnight to prepare a 6.5% Sargassum fusiformis polysaccharide aqueous solution, take 10 portions of the Sargassum fusiformis polysaccharide aqueous solution and place them in a rectangular container equipped with parallel electrodes, the parallel electrodes are spaced 7.5 cm apart, and both ends of the electrodes are connected to the positive and negative poles of an AC power supply, so that the Sargassum fusiformis polysaccharide aqueous solution immerses the two electrodes and then powers on, first at a voltage of 4V for 3 minutes, then at a voltage of 8V for 4 minutes, the voltage is selected in a pulse mode, the power is on for 10 seconds, and then the power is turned off for 10 seconds, to obtain an electrically treated Sargassum fusiformis polysaccharide solution; S2.2: Place 1.6 parts of collagen tripeptide in 10 parts of distilled water at 40°C, stir at a stirring speed of 800 rpm for 1 hour, and keep at 3°C ​​overnight to obtain a collagen tripeptide solution. Add the collagen tripeptide solution into a syringe with a needle diameter of 0.6 mm, and inject it into the electro-treated Sargassum fusiformis polysaccharide solution obtained in step S2.1 at a temperature of 60°C. Then, perform magnetic stirring at a stirring speed of 1000 rpm for 10 minutes to obtain a composite Sargassum fusiformis polysaccharide stabilizer.

[0022] S3: Compound preparation of Chinese herbal medicine extracts S3.1: Pick the reddish Pistacia chinensis flowers, select and remove the corrupt and old parts, then dry them in a clean and dust-free environment, crush them and pass them through an 80-mesh sieve to obtain Pistacia chinensis flower powder, put the Pistacia chinensis flower powder in a container, add a 70% ethanol solution at a solid-liquid ratio of 1:20, heat it to 55°C, and then ultrasonicate it at a power of 250W for 40 minutes, and obtain an extract after drying, disperse the extract with 15 times the volume of pure water, and then add an equal volume of ethyl acetate for extraction, filter to remove the precipitate, and retain the water layer to dry, so as to obtain Pistacia chinensis flower extract; S3.2: Mix 1.2 parts of lemon balm extract, 1.6 parts of Pistacia flower extract and 0.8 parts of mulberry extract, and then add them to the composite Sargassum fusiformis polysaccharide stabilizer prepared in step S2.2, add 0.5wt% of phenoxyethanol, stir at a stirring speed of 1000 rpm for 20 minutes, then sterilize using ultra-high temperature instantaneous sterilization technology, and store in sealed cans to obtain an anti-aging composition.

[0023] Example 3: An anti-aging composition for increasing dermal collagen and a preparation method thereof, such as Figure 1 As shown, the following steps are included: S1: Preparation of Sargassum fusiformis polysaccharide S1.1: Wash 1 portion of Sargassum fusiformis with water to remove salt, freeze-dry, grind into powder and extract with 95% ethanol solution, repeat three times and dry to obtain pretreated Sargassum fusiformis powder, disperse the pretreated Sargassum fusiformis powder with 10 portions of deionized water, adjust the pH to 4.5, add 0.5wt% of cellulase and 0.1wt% of sodium citrate, extract at 55°C with shaking for 20 hours, and boil in hot water at 98°C for 15 minutes to obtain enzymatically hydrolyzed Sargassum fusiformis; S1.2: Add 0.03 parts of calcium chloride to the enzymatically hydrolyzed Sargassum obtained in step S2.1, stir and precipitate, centrifuge and separate after no precipitate is generated, collect the supernatant, adjust the pH to neutral, and then concentrate to 1 / 3 of the original volume, add 5 times the volume of ethanol, and let stand at 4°C for 10 hours. Collect the precipitate by centrifugation and freeze-dry it to obtain Sargassum polysaccharide.

[0024] S2: Preparation of composite Sargassum polysaccharide stabilizer S2.1: Stir the Sargassum fusiformis polysaccharide in distilled water at 70°C until it is completely dissolved, and keep it at 3°C ​​overnight to prepare an 8% Sargassum fusiformis polysaccharide aqueous solution, take 8 portions of the Sargassum fusiformis polysaccharide aqueous solution and place them in a rectangular container equipped with parallel electrodes, the parallel electrodes are spaced 9 cm apart, and both ends of the electrodes are connected to the positive and negative poles of an AC power supply, so that the Sargassum fusiformis polysaccharide aqueous solution immerses the two electrodes and then powers on, first at a voltage of 6V for 4 minutes, then at a voltage of 8V for 5 minutes, the voltage is selected in a pulse mode, the power is on for 15 seconds, and then the power is turned off for 15 seconds, to obtain an electrically treated Sargassum fusiformis polysaccharide solution; S2.2: Place 1.2 parts of collagen tripeptide in 8 parts of distilled water at 45°C, stir at a stirring speed of 1000 rpm for 1.5 hours, and keep at 4°C overnight to obtain a collagen tripeptide solution. Add the collagen tripeptide solution into a syringe with a needle diameter of 0.8 mm, and inject it into the electro-treated Sargassum fusiformis polysaccharide solution obtained in step S2.1 at a temperature of 65°C. Then, perform magnetic stirring at a stirring speed of 1200 rpm for 15 minutes to obtain a composite Sargassum fusiformis polysaccharide stabilizer.

[0025] S3: Compound preparation of Chinese herbal medicine extracts S3.1: Pick the reddish Pistacia chinensis flowers, select and remove the corrupt and old parts, then dry them in a clean and dust-free environment, crush them and pass them through a 90-mesh sieve to obtain Pistacia chinensis flower powder, take 5 parts of Pistacia chinensis flower powder and put them in a container, add 75% ethanol solution at a solid-liquid ratio of 1:20, heat it to 60°C, and then ultrasonicate it at a power of 300W for 50 minutes, and obtain an extract after drying, disperse the extract with 20 times the volume of pure water, and then add an equal volume of n-butanol for extraction, filter to remove the precipitate, retain the water layer and dry it to obtain Pistacia chinensis flower extract; S3.2: Mix 0.6 parts of lemon balm extract, 1.2 parts of Pistacia flower extract and 0.4 parts of mulberry extract, and then add them to the composite Sargassum fusiformis polysaccharide stabilizer prepared in step S2.2, add 0.4wt% of phenoxyethanol, stir at a stirring speed of 1200 rpm for 30 minutes, then sterilize using ultra-high temperature instantaneous sterilization technology, and store in sealed cans to obtain an anti-aging composition.

[0026] Comparative Example 1: The difference between Comparative Example 1 and Example 1 is that in Comparative Example 1, the Pistacia chinensis flower extract in step S3.2 is replaced with an equal amount of honeysuckle extract, and the remaining steps are the same as Example 1 to prepare an anti-aging composition, which is recorded as Comparative Example 1.

[0027] Comparative Example 2: The difference between Comparative Example 2 and Example 1 is that in Comparative Example 2, the lemon balm extract in step S3.2 is replaced with an equal amount of Centella asiatica extract, and the remaining steps are the same as Example 1 to prepare an anti-aging composition, which is recorded as Comparative Example 2.

[0028] Comparative Example 3: The difference between Comparative Example 3 and Example 1 is that in Comparative Example 3, the mulberry extract in step S3.2 is replaced with an equal amount of grape seed extract, and the remaining steps are the same as those in Example 1 to prepare an anti-aging composition, which is recorded as Comparative Example 3.

[0029] Comparative Example 4: The difference between Comparative Example 4 and Example 1 is that in Comparative Example 4, the collagen tripeptide in step S2.2 is replaced with an equal amount of collagen peptide, and the remaining steps are the same as Example 1 to prepare an anti-aging composition, which is recorded as Comparative Example 4.

[0030] Comparative Example 5: The difference between Comparative Example 5 and Example 1 is that, in Comparative Example 5, the Sargassum polysaccharide in step S2 is replaced by an equal amount of chitosan to obtain a composite chitosan stabilizer, and the composite Sargassum polysaccharide stabilizer in step S3.2 is replaced by a composite chitosan stabilizer, and the remaining steps are the same as in Example 1 to obtain an anti-aging composition, which is recorded as Comparative Example 5.

[0031] Comparative Example 6: The difference between Comparative Example 6 and Example 1 is that Comparative Example 6 removes step S2.1, stirs the Sargassum fusiformis polysaccharide in distilled water at 70°C until it is completely dissolved, and keeps it at 3°C ​​overnight to prepare an 8% Sargassum fusiformis polysaccharide aqueous solution, and replaces the electro-treated Sargassum fusiformis polysaccharide solution in step S2.2 with an equal amount of Sargassum fusiformis polysaccharide aqueous solution to prepare a composite Sargassum fusiformis polysaccharide stabilizer. The remaining steps are the same as in Example 1 to prepare an anti-aging composition, which is recorded as Comparative Example 6.

[0032] Experiment 1: Take equal masses of the anti-aging compositions prepared in Examples 1-3 and Comparative Examples 1-5, mix them evenly with the DPPH standard solution at a mass ratio of 1:3 at room temperature, let stand for 35 minutes, repeat the parallel experiment, and record them as the first experimental group and the second experimental group, respectively. Mix deionized water and the DPPH standard solution at a mass ratio of 1:3, let stand for 35 minutes, as the blank group, read the absorbance at 516nm of the ultraviolet spectrophotometer, and calculate the DPPH free radical scavenging rate, DPPH free radical scavenging rate = 1-absorbance of the experimental group / absorbance of the blank group × 100%, record the data and make a table, as shown in Table 1.

[0033] Table 1: DPPH free radical scavenging rate of anti-aging composition

[0034] It can be seen from the data of Examples 1-3 in Table 1 that the anti-aging compositions prepared using lemon balm extract, Pistacia flower extract, mulberry extract, collagen tripeptide and Sargassum fusiformis polysaccharide all have excellent free radical scavenging effects within the formula composition, and it can be seen from the data of Comparative Examples 1-5 that when one of the components is replaced with an equal amount of an extract with similar properties, the free radical scavenging effect of the anti-aging composition is reduced, proving that there is a synergistic effect between the components of the anti-aging composition provided in this embodiment, which can effectively scavenge free radicals and inhibit oxidative stress.

[0035] Experiment 2: Take equal masses of the anti-aging compositions prepared in Examples 1-3 and Comparative Examples 1-5, add 5% white sugar flavoring, stir well to prepare samples, take 54 SD rats, half male and half female, weighing 200±20 g, and divide them into 9 groups, 6 rats in each group, one group as a blank group, and the remaining groups as experimental groups are respectively administered by gavage with samples prepared from the anti-aging compositions of Examples 1-3 and one of the samples prepared from Comparative Examples 1-5, the dosage of which is 40 mg / kg based on the Pistacia chinensis flower extract, once a day for 30 days.

[0036] On the 31st day, the back skin of each group of mice was carefully depilated and cleaned, and then the mice were killed by cervical dislocation. 100 mg of skin tissue was accurately weighed from each mouse in each group, and the skin tissues obtained from each group of mice were mixed and cut into minced meat to obtain meat tissue. Three portions of 100 mg of meat tissue were taken from each group. The meat tissues were processed according to GB / T9695.23-2008 and the hydroxyproline content therein was determined and calculated. Hydroxyproline is a unique component of collagen, accounting for about 13.4% of the total amino acids in collagen. The higher the hydroxyproline content, the higher the collagen content. The collagen content was calculated as hydroxyproline content / 13.4%. The data were recorded and tabulated, as shown in Table 2.

[0037] Table 2: Collagen content in mouse skin after feeding different samples for 30 days

[0038] Compared with the blank group, it can be seen that the anti-aging composition prepared in the embodiment can significantly increase the collagen content in the mouse skin, which can prove that the anti-aging composition prepared using lemon balm extract, Pistacia flower extract, mulberry extract, collagen tripeptide and Sargassum fusiformis polysaccharide in the formula composition can increase the collagen content in the dermis, and it can be seen from the data of comparative examples 1-5 that when one of the components is replaced with an equal amount of an extract with similar properties, the hydroxyproline content in the mouse skin is reduced, which proves that there is a synergistic effect between the components of the anti-aging composition provided in this embodiment, which can better promote the secretion of collagen in the skin.

[0039] Experiment 3: Take equal masses of the anti-aging composition prepared in Example 1 and comparative examples 5-6, add 5% white sugar flavoring, stir evenly to prepare sample 1-sample 3 and comparative sample 1-comparative sample 5, place them in a stabilization box at a temperature of 37°C ± 2°C and a relative humidity of 70% ± 5% for 3 months, take samples at the first, second and third months, detect the content of the active ingredient in the sample solution, record the data and make a table, as shown in Table 3.

[0040] Table 3: Content of active ingredients in anti-aging composition samples during 3-month storage

[0041] It can be seen that the content of active ingredients in the anti-aging composition prepared in Example 1 hardly changes within three months, and from the data of Comparative Examples 5-6, it can be seen that replacing the Sargassum fusiformis polysaccharide with chitosan and not conducting electrical treatment will reduce the stability of the system, thereby reducing the content of active ingredients. It can be proved that the use of Sargassum fusiformis polysaccharide and electrical treatment can both improve the stability of the system, ensuring that the active ingredients of the subsequently prepared anti-aging composition are not easily precipitated and degraded when made into an oral liquid, thereby avoiding insufficient nutrient utilization.

[0042] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A method for preparing an anti-aging composition for increasing dermal collagen, characterized in that: The following steps are involved: S1: Preparation of Sargassum fusiformis polysaccharide The method comprises the following steps: desalting, freeze-drying and grinding the Sargassum fusiformis into powder, extracting the powder with ethanol, dispersing the powder with deionized water and adjusting the pH for enzymatic hydrolysis, adding sodium citrate during the enzymatic hydrolysis to obtain enzymatically hydrolyzed Sargassum fusiformis, adding calcium chloride for precipitation, performing low-temperature alcohol precipitation and freeze-drying to obtain Sargassum fusiformis polysaccharide; S2: Preparation of composite Sargassum polysaccharide stabilizer Dissolving Sargassum fusiformis polysaccharide in distilled water at low temperature overnight, and then subjecting it to electric treatment in a pulse current to obtain an electric-treated Sargassum fusiformis polysaccharide solution, dissolving collagen tripeptide in distilled water at low temperature overnight to obtain a collagen tripeptide solution, injecting the collagen tripeptide solution into the electric-treated Sargassum fusiformis polysaccharide solution, and then subjecting it to magnetic stirring to obtain a composite Sargassum fusiformis polysaccharide stabilizer; S3: Compound preparation of Chinese herbal medicine extracts The reddened Pistacia flowers are picked, the corrupt and old parts are removed, and then they are dried and crushed. Then, ultrasonic extraction is carried out in ethanol. After drying, they are dispersed with pure water, and then organic solvent extraction is carried out. The water layer is retained and dried to obtain the Pistacia flower extract. The lemon balm extract, the Pistacia flower extract and the mulberry extract are added to the composite Sargassum fusiformis polysaccharide stabilizer and stirred evenly. After sterilization, the anti-aging composition is canned and sealed.

2. The method for preparing an anti-aging composition for increasing dermal collagen according to claim 1, characterized in that: Step S1: Preparation of Sargassum fusiformis polysaccharide, comprising the following steps: S1.1: 1-1.5 parts of Sargassum fusiformis are washed and desalted, freeze-dried, ground into powder, and extracted with an ethanol solution having a concentration of 92-95%, the extraction is repeated three times, and then dried to obtain pretreated Sargassum fusiformis powder, the pretreated Sargassum fusiformis powder is dispersed with 10-12 parts of deionized water, the pH is adjusted to 4-4.5, 0.5-0.6 wt % of cellulase and 0.1-0.2 wt % of sodium citrate are added, the mixture is shaken and extracted at 50-55° C. for 18-20 hours, and then boiled in hot water at 95-98° C. for 12-15 minutes to obtain enzymolyzed Sargassum fusiformis; S1.2: Add 0.02-0.03 parts of calcium chloride to the enzymolyzed Sargassum obtained in step S2.1, stir and precipitate, centrifuge and separate after no precipitate is generated, collect the supernatant, adjust the pH to neutral, and then concentrate to 1 / 4-1 / 3 of the original volume, add 4-5 times the volume of ethanol, and let stand at 3-4°C for 8-10 hours. Collect the precipitate by centrifugation and freeze-dry it to obtain Sargassum polysaccharide.

3. The method for preparing an anti-aging composition for increasing dermal collagen according to claim 2, characterized in that: Step S2 prepares a composite Sargassum fusiformis polysaccharide stabilizer, comprising the following steps: S2.1: Stir the Sargassum fusiformis polysaccharide in distilled water at 65-70°C until it is completely dissolved, and keep it at 3-4°C overnight to prepare a Sargassum fusiformis polysaccharide aqueous solution with a concentration of 6.5-8%, take 8-10 portions of the Sargassum fusiformis polysaccharide aqueous solution and place it in a rectangular container equipped with parallel electrodes, the parallel electrodes are spaced 7.5-9 cm apart, and both ends of the electrodes are connected to the positive and negative poles of an AC power supply, so that the Sargassum fusiformis polysaccharide aqueous solution immerses the two electrodes and then powers on, first at a voltage of 4-6V for 3-4 minutes, then at a voltage of 8-10V for 4-5 minutes, the voltage is selected in a pulse mode, the power is on for 10-15 seconds, and then the power is turned off for 10-15 seconds, to obtain an electrically treated Sargassum fusiformis polysaccharide solution; S2.2: Place 1.2-1.6 parts of collagen tripeptide in 8-10 parts of distilled water at 40-45°C, stir at a stirring speed of 800-1000rpm for 1-1.5 hours, and keep at 3-4°C overnight to obtain a collagen tripeptide solution. Add the collagen tripeptide solution into a syringe and inject it into the electro-treated Sargassum fusiformis polysaccharide solution obtained in step S2.1 at a temperature of 60-65°C, and then magnetically stir at a stirring speed of 1000-1200rpm for 10-15 minutes to obtain a composite Sargassum fusiformis polysaccharide stabilizer.

4. The method for preparing an anti-aging composition for increasing dermal collagen according to claim 3, characterized in that: Step S3: compound preparation of Chinese herbal medicine extracts, comprising the following steps: S3.1: Pick the reddish Pistacia chinensis flowers, select and remove the corrupt and old parts, then dry them in a clean and dust-free environment, crush them and pass them through an 80-90 mesh sieve to obtain Pistacia chinensis flower powder, put the Pistacia chinensis flower powder in a container, add 70-75% ethanol solution at a solid-liquid ratio of 1: (18-20), heat it to 55-60°C, and then ultrasonicate it at a power of 250-300W for 40-50 minutes, and obtain an extract after drying, disperse the extract with 15-20 times the volume of pure water, and then add an equal volume of organic solvent for extraction, filter to remove the precipitate, retain the water layer and dry it to obtain Pistacia chinensis flower extract; S3.2: Mix 0.6-1.2 parts of lemon balm extract, 1.2-1.6 parts of Pistacia flower extract and 0.4-0.8 parts of mulberry extract, and then add them to the composite Sargassum fusiformis polysaccharide stabilizer prepared in step S2.2, add 0.4-0.5wt% of phenoxyethanol, stir at a stirring speed of 1000-1200rpm for 20-30 minutes, then sterilize by ultra-high temperature instantaneous sterilization technology, and store in sealed cans to obtain an anti-aging composition.

5. The method for preparing an anti-aging composition for increasing dermal collagen according to claim 3, characterized in that: The syringe needle in step S2.2 has a diameter of 0.6-0.8 mm.

6. The method for preparing an anti-aging composition for increasing dermal collagen according to claim 4, characterized in that: The organic solvent in step S3.1 is one of petroleum ether, ethyl acetate or n-butanol.

7. An anti-aging composition for increasing collagen in the dermis, characterized in that: The composition is prepared by the method for preparing an anti-aging composition for increasing dermal collagen as described in any one of claims 1 to 6.

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