Hypoglycemic health-care product rich in bitter gourd polypeptide and application of hypoglycemic health-care product

Abstract

The invention relates to the technical field of health-care products, in particular to a health-care product rich in momordica charantia polypeptide and capable of regulating blood sugar metabolism and promoting insulin secretion, momordica charantia polypeptide can stimulate pancreatic beta cells to secrete insulin, and insulin is the only hormone capable of reducing blood sugar in a human body. The balsam pear polypeptide component in the balsam pear extract is specially used for reducing the blood sugar level and improving related health problems, the application of the balsam pear polypeptide in the health care product can effectively regulate the blood sugar metabolism of a human body and promote the secretion of insulin, and the balsam pear polypeptide stimulates pancreas islet beta cells to promote the secretion of insulin. In addition, the balsam pear polypeptide can improve the insulin resistance, increase the number of insulin receptors or enhance the activity of the insulin receptors, so that the body cells are more sensitive to the insulin, the uptake and utilization of glucose are promoted, and the abnormal increase of the blood glucose level is avoided.

Description

Technical Field

[0001] The invention relates to the technical field of health care products, and in particular to a blood sugar lowering health care product rich in bitter melon polypeptide and application thereof. Background Art

[0002] Bitter melon (Momord i cacharantia L.) is a plant of the Cucurbitaceae family. The whole plant, including the fruit, has a bitter taste. The leaf margins are serrated, the fruit is rectangular, the young fruit is emerald green, and it turns orange-yellow when mature. It grows in subtropical areas and is widely planted worldwide because it can be used as both medicine and vegetable. The fruit is bitter and cold in taste. It has the effects of clearing away summer heat, clearing away heat, improving eyesight and detoxifying. It can be applied topically to treat trauma, and taken orally for anthelmintic, regulating menstruation, antiviral, and treating measles and hepatitis. Pharmacological experiments have confirmed that it has the effects of treating diabetes, antiviral, anticancer, antibacterial and insecticidal. A Chinese patent discloses a bitter melon polypeptide and its preparation method and application (authorization announcement number CN101985644B), which discloses a bitter melon polypeptide and its preparation method and application. The bitter melon polypeptide is prepared according to a method comprising the following steps: using papain to enzymolyze bitter melon powder to obtain the bitter melon polypeptide. Its relative molecular mass is mainly between 130 and 1000Da, and it is composed of 2 to 9 amino acids. It is very easy for the human body to absorb, can be quickly utilized by tissues, quickly balance blood sugar, and can be taken for a long time. The bitter melon polypeptide provided by the present invention can be used as a pharmaceutical raw material and a food additive for the preparation of products for the prevention and / or treatment of diabetes, such as medicines, health products, foods, etc. The method for preparing bitter melon polypeptides of the invention has a simple process, low investment, quick effect, high added value, and is suitable for industrial production. The patented technology solves the problem that at present, both domestic and foreign countries are keen on using bitter melon to treat diabetes, and many products such as "bitter melon extract", "bitter melon total saponin", "bitter melon tea", "bitter melon powder" and so on have been successively developed, but the relative molecular mass of the effective substances in these products is large, and it is not easy for the human body to absorb and utilize them. Products like "bitter melon powder", "bitter melon tea" and "bitter melon extract" are not suitable for people with cold constitutions or should not eat more or eat them often, according to the theory of traditional Chinese medicine.

[0003] However, the prior art lacks the application of bitter melon polypeptides in blood pressure lowering health products, and it is necessary to solve the problem that bitter melon polypeptides rich in the prior art can be used in blood sugar lowering health products.

[0004] Therefore, those skilled in the art provide a blood sugar lowering health care product rich in bitter melon polypeptide and its application to solve the problems raised in the above background technology. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides the application of bitter melon extract rich in bitter melon polypeptides to blood sugar lowering health care products, that is, the application of bitter melon polypeptides in blood pressure lowering health care products;

[0006] The blood sugar lowering health care product rich in bitter melon polypeptide provided by the present invention has the effects of regulating blood sugar metabolism and promoting insulin secretion. Bitter melon polypeptide can stimulate pancreatic β cells to secrete insulin. Insulin is the only hormone in the human body that can lower blood sugar. It can promote the uptake and utilization of glucose by cells, thereby lowering blood sugar levels and allowing glucose to smoothly enter cells and be utilized or stored by cells, rather than accumulating in the blood to cause blood sugar to rise.

[0007] The blood sugar lowering health care product rich in bitter melon polypeptide provided in the present invention has the effect of improving insulin resistance. Insulin resistance refers to the reduced sensitivity of body cells to insulin. Bitter melon polypeptide can increase the number of insulin receptors or enhance the activity of receptors, so that insulin can play a better role, making cells more receptive to insulin, facilitating the uptake of glucose, and making the blood sugar regulation process smoother.

[0008] The bitter melon polypeptide provided in the present invention has an antioxidant effect on the blood sugar lowering health care product. The bitter melon polypeptide contains rich antioxidant ingredients and can remove free radicals in the body. Free radicals are unstable molecules that can damage cells and tissues and affect the normal function of cells, including insulin-secreting cells. Excessive free radicals will attack pancreatic islet cells and interfere with the synthesis and secretion of insulin. The bitter melon polypeptide protects the integrity and function of pancreatic islet cells by removing these free radicals, thereby helping to maintain normal blood sugar levels; inhibit sugar absorption. In the gastrointestinal tract, the bitter melon polypeptide can inhibit the activity of some carbohydrate hydrolases, such as α-glucosidase, which breaks down carbohydrates into glucose, which is absorbed by the human body. By inhibiting these enzymes, the bitter melon polypeptide can reduce the generation and absorption of glucose and reduce the increase in blood sugar after a meal.

[0009] Bitter melon peptides can regulate the function of the immune system. They can promote the proliferation and activity of immune cells (such as lymphocytes, macrophages, etc.) and enhance the body's immune defense capabilities. At the same time, bitter melon peptides can also regulate the secretion of immunoglobulins, helping the body to better identify and eliminate foreign pathogens, such as bacteria and viruses;

[0010] Bitter melon polypeptide can promote the secretion of digestive enzymes in the gastrointestinal tract, such as protease and amylase, which help to decompose and digest food. In addition, bitter melon polypeptide also has a certain protective effect on the gastrointestinal mucosa, which can prevent excessive damage to the gastric mucosa by gastric acid and pepsin;

[0011] Bitter melon peptides may play an auxiliary role in anti-tumor through multiple pathways. It can induce apoptosis of tumor cells, that is, it can cause tumor cells to die according to their own program. At the same time, bitter melon peptides can also inhibit the proliferation and metastasis of tumor cells, and interfere with the growth cycle of tumor cells by regulating cell signaling pathways. In addition, bitter melon peptides may also enhance the body's immune surveillance function, allowing the immune system to better identify and attack.

[0012] Technical effects and advantages of the present invention:

[0013] The present invention utilizes the bitter melon polypeptide component in the bitter melon extract, specifically for lowering blood sugar levels and improving related health problems. The application of the bitter melon polypeptide in this health product can not only effectively regulate the human body's blood sugar metabolism, but also promote the secretion of insulin. The bitter melon polypeptide stimulates pancreatic beta cells to help these cells release more insulin, thereby lowering the blood sugar level. In addition, the bitter melon polypeptide can also improve insulin resistance, increase the number of insulin receptors or enhance their activity, make body cells more sensitive to insulin, thereby promoting the uptake and utilization of glucose and avoiding abnormal increases in blood sugar levels.

[0014] In terms of anti-oxidation, the bitter melon polypeptide of the present invention is rich in antioxidant ingredients, which can remove free radicals in the body, protect pancreatic cells from damage, and maintain their normal functions. The bitter melon polypeptide can also inhibit the activity of certain carbohydrate hydrolases in the gastrointestinal tract, such as α-glucosidase, reduce the production and absorption of glucose, and thus reduce the increase in postprandial blood sugar. In terms of the immune system, the bitter melon polypeptide can promote the proliferation and activity of immune cells, enhance the body's immune defense capabilities, and regulate the secretion of immunoglobulins, helping the body to more effectively identify and eliminate foreign pathogens.

[0015] The bitter melon polypeptide in the present invention promotes the secretion of digestive enzymes in the gastrointestinal tract, such as protease, amylase, etc., which helps to decompose and digest food. It also has the function of protecting the gastrointestinal mucosa and preventing excessive damage to the gastric mucosa by gastric acid and pepsin. In terms of anti-tumor, the bitter melon polypeptide may play a role through multiple mechanisms, including inducing tumor cell apoptosis, inhibiting tumor cell proliferation and metastasis, and enhancing the body's immune surveillance function, helping the immune system to more effectively identify and attack tumor cells. DETAILED DESCRIPTION

[0016] The present invention is further described in detail below in conjunction with specific embodiments. The examples of the present invention are provided for the purpose of illustration and description, and are not intended to be exhaustive or to limit the present invention to the disclosed forms. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments are selected and described in order to better illustrate the principles and practical applications of the present invention, and to enable those of ordinary skill in the art to understand the present invention and thereby design various embodiments with various modifications suitable for specific uses.

[0017] Embodiment 1: In this embodiment, a blood sugar lowering health care product rich in bitter melon polypeptide and its application are provided. The blood sugar lowering health care product rich in bitter melon polypeptide involved in the present invention has the function of regulating blood sugar metabolism and can promote the secretion of insulin. Bitter melon polypeptide can stimulate pancreatic β cells to secrete insulin, and insulin, as the only hormone in the human body that can lower blood sugar, can promote the cell's uptake and utilization of glucose, thereby lowering blood sugar levels, ensuring that glucose can smoothly enter the cell for cell use or storage, and avoiding its accumulation in the blood, which leads to increased blood sugar;

[0018] The blood sugar lowering health care product rich in bitter melon polypeptides involved in the present invention also has the function of improving insulin resistance. Insulin resistance refers to the reduced sensitivity of body cells to insulin. Bitter melon polypeptides can increase the number of insulin receptors or enhance the activity of receptors, so that insulin can work more effectively, make cells more receptive to insulin, promote glucose uptake, and thus make the blood sugar regulation process smoother;

[0019] The blood sugar lowering health care product rich in bitter melon polypeptides involved in the present invention also has an antioxidant effect. Bitter melon polypeptides are rich in antioxidant ingredients and can remove free radicals in the body. Free radicals are unstable molecules that can damage cells and tissues and affect the normal function of cells, including insulin-secreting cells. Excessive free radicals can attack pancreatic islet cells and interfere with the synthesis and secretion of insulin. Bitter melon polypeptides protect the integrity and function of pancreatic islet cells by removing these free radicals, thereby helping to maintain normal blood sugar levels; inhibit sugar absorption. In the gastrointestinal tract, bitter melon polypeptides can inhibit the activity of some carbohydrate hydrolases, such as α-glucosidase, which breaks down carbohydrates into glucose, which is then absorbed by the human body. By inhibiting these enzymes, bitter melon polypeptides can reduce the production and absorption of glucose and reduce the increase in blood sugar after a meal.

[0020] Bitter melon polypeptide can regulate the function of the immune system. It can promote the proliferation and activity of immune cells (such as lymphocytes, macrophages, etc.) and enhance the body's immune defense capabilities. At the same time, bitter melon polypeptide can also regulate the secretion of immunoglobulins, helping the body to better identify and eliminate foreign pathogens, such as bacteria and viruses;

[0021] Bitter melon polypeptide can promote the secretion of digestive enzymes in the gastrointestinal tract, such as protease and amylase, which help to decompose and digest food. In addition, bitter melon polypeptide also has a certain protective effect on the gastrointestinal mucosa, which can prevent excessive damage to the gastric mucosa by gastric acid and pepsin;

[0022] Bitter melon peptides may play an auxiliary role in anti-tumor through multiple pathways. It can induce apoptosis of tumor cells, that is, it can cause tumor cells to die according to their own program. At the same time, bitter melon peptides can also inhibit the proliferation and metastasis of tumor cells, and interfere with the growth cycle of tumor cells by regulating cell signaling pathways. In addition, bitter melon peptides may also enhance the body's immune surveillance function, allowing the immune system to better identify and attack.

[0023] Example 2: In this example, a method for extracting and preparing bitter melon polypeptides from bitter melon in a blood sugar-lowering health product rich in bitter melon polypeptides is provided;

[0024] Extraction and preparation method of bitter melon polypeptides from bitter melon in blood sugar-lowering health products:

[0025] Step 1: Select fresh and intact bitter melon;

[0026] Step 2: After removing the roots and stems of the bitter melon selected in step 1, place it in a cleaning machine to clean the dirt residue on the surface, and then drain the remaining water stains;

[0027] Step 3: placing the bitter melon after draining the water in step 2 into a freeze dryer to pre-freeze the bitter melon, the pre-freezing time is 2 to 3 hours, the temperature is -15°C to -20°C, the pre-frozen bitter melon can reduce the deformation of the bitter melon during vacuum sublimation drying, and the post-freeze drying freezing time is 2 to 4 hours at a temperature of -30°C to -40°C;

[0028] The vacuum degree in the post-freeze dryer is 10 to 100 Pascals, the temperature is -20°C to -10°C, and after 12 to 24 hours of freezing processing, the moisture content of the bitter melon is reduced to below 10%;

[0029] In the drying stage, the temperature of the freeze dryer rises to 0℃~10℃ to remove the residual moisture adsorbed in the bitter melon. The drying time is 2~4h. As the temperature rises, the activity of water molecules in the material increases, and the residual moisture in the bitter melon is removed, and the moisture content of the bitter melon is reduced to an extremely low level of 3%~5% or less.

[0030] Step 4: placing the bitter melon after removing the water in step 3 in a grinder to grind into bitter melon powder, and sieving the bitter melon powder to a mesh size of 80 to 100;

[0031] Step 5: placing the bitter melon powder prepared in step 4 into an enzymolysis tank, adding papain, and heating the temperature to 40-50° C. for 45-55 minutes, so that the bitter melon powder undergoes enzymolysis reaction in the heat-insulating enzymolysis tank with papain, and the bitter melon protein is degraded into polypeptides;

[0032] Step 6: Degrading the bitter melon polypeptide solution formed in step 5 is placed in a cold storage at a temperature of -2 to 0°C for a precipitation time of 120 to 160 hours. During the precipitation process, oxalic acid and metal ions are conducive to forming insoluble salts and separating from the bitter melon polypeptide solution;

[0033] The precipitated bitter melon polypeptide solution is filtered using a filtering device to remove insoluble matter and impurities, and the filtration is performed using a 0.5 μm to 0.2 μm filter membrane, and the filtration is repeated until the bitter melon polypeptide solution is clear;

[0034] Step 7: The clear bitter melon polypeptide solution in step 6 is treated by enzyme inactivation in a water bath at a temperature of 85°C to 90°C for 1 to 1.5 hours. The bitter melon polypeptide solution after inactivation is sterilized by high temperature at 100°C.

[0035] Step 8: concentrating the bitter melon polypeptide solution disinfected in step 7, and then drying the concentrated bitter melon polypeptide solution into powder using a spray dryer, and then crushing and sieving to obtain bitter melon polypeptide powder that is beneficial to lowering blood sugar;

[0036] Step nine: The bitter melon polypeptide powder obtained in step eight is packaged in capsules to obtain a health product that is beneficial for lowering blood sugar.

[0037] Example 3: In this example, a blood sugar lowering health product rich in bitter melon polypeptide is provided for use in the preparation of health products;

[0038] The bitter melon polypeptide powder is mixed with dried ginger powder before packaging, and the ratio of bitter melon polypeptide powder to dried ginger powder is 10:1 gram;

[0039] The bitter melon polypeptide powder is mixed with cinnamon powder before packaging, and the adding ratio is 10:0.1 grams.

[0040] Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field and related fields without creative work should fall within the scope of protection of the present invention. The structures, devices and operating methods not specifically described and explained in the present invention are implemented according to the conventional means in the field unless otherwise specified and limited.

Claims

1. An application of bitter melon polypeptide-rich product in a blood sugar-lowering health care product, characterized in that: The extraction method of the blood sugar lowering health product rich in bitter melon polypeptides comprises: Extraction and preparation method of bitter melon polypeptides from bitter melon in blood sugar-lowering health products: Step 1: Select fresh and intact bitter melon; Step 2: After removing the roots and stems of the bitter melon selected in step 1, place it in a cleaning machine to clean the dirt residue on the surface, and then drain the remaining water stains; Step 3: placing the bitter melon after draining the water in step 2 into a freeze dryer to pre-freeze the bitter melon, the pre-freezing time is 2 to 3 hours, the temperature is -15°C to -20°C, the pre-frozen bitter melon can reduce the deformation of the bitter melon during vacuum sublimation drying, and the post-freeze drying freezing time is 2 to 4 hours at a temperature of -30°C to -40°C; The vacuum degree in the post-freeze dryer is 10 to 100 Pascals, the temperature is -20°C to -10°C, and after 12 to 24 hours of freezing processing, the moisture content of the bitter melon is reduced to below 10%; In the drying stage, the temperature of the freeze dryer rises to 0℃~10℃ to remove the residual moisture adsorbed in the bitter melon. The drying time is 2~4h. As the temperature rises, the activity of water molecules in the material increases, and the residual moisture in the bitter melon is removed, and the moisture content of the bitter melon is reduced to an extremely low level of 3%~5% or less. Step 4: placing the bitter melon after removing the water in step 3 in a grinder to grind into bitter melon powder, and sieving the bitter melon powder to a mesh size of 80 to 100; Step 5: placing the bitter melon powder prepared in step 4 into an enzymolysis tank, adding papain, and heating the temperature to 40-50° C. for 45-55 minutes, so that the bitter melon powder undergoes enzymolysis reaction in the heat-insulating enzymolysis tank with papain, and the bitter melon protein is degraded into polypeptides; Step 6: Degrading the bitter melon polypeptide solution formed in step 5 is placed in a cold storage at a temperature of -2 to 0°C for a precipitation time of 120 to 160 hours. During the precipitation process, oxalic acid and metal ions are conducive to forming insoluble salts and separating from the bitter melon polypeptide solution; The precipitated bitter melon polypeptide solution is filtered using a filtering device to remove insoluble matter and impurities, and the filtration is performed using a 0.5 μm to 0.2 μm filter membrane, and the filtration is repeated until the bitter melon polypeptide solution is clear; Step 7: The clear bitter melon polypeptide solution in step 6 is treated by enzyme inactivation in a water bath at a temperature of 85°C to 90°C for 1 to 1.5 hours. The bitter melon polypeptide solution after inactivation is sterilized by high temperature at 100°C. Step 8: Concentrating the bitter melon polypeptide solution disinfected in step 7, and then drying the concentrated bitter melon polypeptide solution into powder using a spray dryer, and then crushing and sieving to obtain bitter melon polypeptide powder that is beneficial to lowering blood sugar; Step nine: The bitter melon polypeptide powder obtained in step eight is packaged in capsules to obtain a health product that is beneficial for lowering blood sugar.

2. A health product using the blood sugar lowering health product rich in bitter melon polypeptide according to claim 1, characterized in that: The method comprises mixing the bitter melon polypeptide powder with dried ginger powder before packaging, wherein the ratio of the bitter melon polypeptide powder to the dried ginger powder is 10:1 gram; The bitter melon polypeptide powder is mixed with cinnamon powder before packaging, and the adding ratio is 10:0.1 grams.