26 results about "Collagen secretion" patented technology

The invention provides recombinant IV type collagen and application thereof, and relates to the technical field of protein engineering. In the invention, an amino acid residue repetitive unit with good biological activity and stability in the human-derived IV-type collagen is found, and the amino acid residue repetitive unit is used as a core for repeating and constructing the recombinant IV-type collagen, so that the novel human-derived recombinant IV-type collagen with biological activity is obtained. The human-derived recombinant IV-type collagen provided by the invention is a small-molecular-weight protein, is easy to absorb by a human body, is good in safety, can remarkably promote secretion of collagen, also has the effects of promoting cell adhesion and cell migration, and has a good application prospect in the aspects of basement membrane repair and remodeling. A new functional component is provided for products for repairing and tightening the skin, resisting aging, resisting wrinkles, preserving moisture and the like.

The invention belongs to the field of daily chemicals, and discloses a PDRN-containing composition with firming and anti-wrinkle effects, and the composition comprises PDRN, saururus chinensis extract, vetiver root oil, rusty rose seed oil and ginseng seed oil. According to the composition provided by the invention, the PDRN, the saururus chinensis extract, the vetiver root oil, the rusty rose seed oil and the ginseng seed oil are compounded for use, and the components are mutually matched and synergistically promoted to obtain a synergistic effect in the aspect of promoting the secretion of type I collagen, so that the composition has remarkable tightening and anti-wrinkle effects. In addition, the composition provided by the invention also has a certain barrier repairing effect, can significantly reduce the TEWL value of skin, is mild and non-irritant, and is suitable for people with sensitive skin.

The invention discloses a recombinant III-type collagen and PDRN compound composition and a preparation method thereof, and relates to the technical field of biological medicines. The composition is formed by compounding recombinant human III type collagen and polydeoxyribonucleotide according to the mass ratio of 1: 10-10: 1, the weight-average molecular weight of the recombinant human III type collagen and the weight-average molecular weight of the polydeoxyribonucleotide are 50-100 kDa, the ratio can generate synergistic interaction, fibroblast proliferation, migration and collagen secretion are remarkably promoted, and the activity of the composition is superior to that of a single component and commercially available products. The composition can be used for preparing medical instruments, cosmetics or water-light injections for skin repair, rejuvenation or skin improvement. The invention also provides a water-light injection containing the compound composition, and the obtained preparation is stable, clear, transparent, safe and non-irritant.

This invention provides an anti-aging and repair dressing containing four complex active factors and its preparation method. The dressing comprises 75-90 parts of a matrix material, 5-15 parts of four complex active factors, and 3-8 parts of a moisturizing auxiliary agent. The four complex active factors are composed of recombinant human type III collagen, acetyl hexapeptide-8, ectoine, and dipotassium glycyrrhizate in a ratio of (3-6):(1-3):(2-4):(1-2). The matrix is ​​a hydrophilic nonwoven fabric or bio-cellulose membrane material that has undergone low-temperature plasma surface grafting activation treatment. The preparation process employs nitrogen combined with vacuum pulse freeze-drying technology, and enhances the activity stability through an ectoine complexation step. This dressing can promote HaCaT cell proliferation, increase collagen secretion, and inhibit inflammatory factors, significantly improving skin barrier function. It has good biocompatibility and can be used to prepare products that improve skin aging and repair.

This invention discloses recombinant type III collagen, its preparation method, and its applications. The amino acid sequence of the recombinant type III collagen is shown in SEQ ID NO: 9. The recombinant type III collagen integrates the RGDV highly active motif and the EKEK hydrophilic stable domain with the core structure of type III collagen to achieve an integrated design of "high activity and long-lasting effect". The in vitro cell migration and collagen secretion effects are improved by more than 50% compared with wild type. Through anti-enzyme cleavage site modification and hydrophilic modification, the in vivo residence half-life is significantly prolonged to meet the long-term repair needs of the dermis.

This invention provides a method for obtaining ingredients from Dulong Paris polyphylla (… Paris dulongensis A method for preparing parisyunnanoside G (PFE55) in H. Li & Kurita and its application in cosmetics and pharmaceuticals. This research belongs to the field of cosmetics and pharmaceutical technology. PFE55 is used at extremely low concentrations (0.1... μ At a concentration of g / mL, it exhibits activity in promoting collagen secretion in adult dermal fibroblasts (HDFa) and is non-cytotoxic at this concentration, making it suitable for the preparation of anti-aging drugs or skincare products.

This invention belongs to the field of biomedical technology, specifically relating to the application of mesenchymal stem cells in the preparation of anti-aging drugs. The mesenchymal stem cells are human adipose-derived mesenchymal stem cells (hAMSCs) or mouse bone marrow mesenchymal stem cells (BM-MSCs). This invention utilizes mesenchymal stem cells to regulate senescent stromal cells and the spleen's ECM, improving the thinning stromal state in the tissue microenvironment through multiple targets. Specifically, it improves the aging-related secretory phenotype of stromal cells, enhances mitochondrial activity, strengthens their proliferation capacity, and delays the reduction of collagen secretion. This, in turn, delays the disordered distribution and functional decline of immune cells caused by aging and enhances tissue regeneration capacity, indirectly affecting various other cells in the tissue microenvironment, thus exhibiting an antagonistic effect against systemic organ aging.

The traditional Chinese medicine mouthwash comprises a traditional Chinese medicine compound and a mouthwash matrix, and the traditional Chinese medicine compound comprises the following components in parts by weight: 10-20 parts of golden cypress, 15-30 parts of angelica sinensis, 15-30 parts of honeysuckle, 15-30 parts of chrysanthemum and 15-30 parts of liquorice. The invention also provides a preparation method of the traditional Chinese medicine mouthwash for relieving gingivitis. The traditional Chinese medicine mouthwash for relieving gingivitis can promote proliferation of gingival fibroblasts, promote secretion of gingival fibroblast collagen and effectively inhibit proliferation of oral anaerobic bacteria, thereby relieving gingivitis.

The application relates to a recombinant type I collagen and application thereof, which utilizes the characteristics of Pichia pastoris capable of secreting and expressing recombinant proteins, secretes the recombinant type I collagen on the culture medium supernatant, and reduces the difficulty of protein purification. In addition, the integrin site RGD is inserted into the collagen to form a recombinant type I collagen with high stability and high expression. According to the relative proliferation of cells and the relative adhesion experiment of cells, it can be seen that the recombinant type I collagen has the effects of promoting the proliferation of skin fibroblasts, improving the skin, promoting wound healing, promoting the adhesion of fibroblasts in the skin layer and the like. Therefore, the recombinant type I collagen has great potential applications in the fields of skin care and beauty, medical treatment and the like.

The invention discloses application of recombinant human glaze matrix protein in skin repair dressing, and belongs to the technical field of biomedicine. The skin repair dressing comprises recombinant human glaze matrix protein, sodium hyaluronate, chondroitin sulfate, osa starch and propylgallate wrapped by lipidosome, and the repair effect is improved through a multiple synergistic mechanism. The preparation method of the dressing comprises the following steps: mixing the recombinant human glaze matrix protein with the stabilizer, and then carrying out homogeneous binding with the sodium hyaluronate, the osa starch, the liposome propyl gallate and the hydrogel matrix. Experiments prove that after the dressing is used, the wound healing speed is increased, the III type collagen secretion amount is increased, the TGF-beta1 expression is reduced, the scar index is reduced, and the dressing is suitable for burn and surgical incision repair.

The invention relates to the technical field of protein engineering or genetic engineering, and particularly discloses a method for improving the secretory expression efficiency of recombinant III-type collagen (Col3) in pichia pastoris in a fermentation tank. The invention discloses pichia pastoris subjected to fermentation culture. The pichia pastoris simultaneously expresses a type III collagen gene, a secretion signal peptide gene and a gene expression regulation element. The pichia pastoris is subjected to four-stage fermentation culture (not traditional three-stage fermentation culture), high expression of recombinant III type collagen in a fermentation tank can be achieved, and the yield is at least 10 g / L or above. The technical scheme provided by the invention has the effect of greatly improving the fermentation yield of the recombinant III type collagen.

The invention provides a polymer microsphere as well as a preparation method and application thereof. The polymer microspheres contain a first polyester material and a second polyester material, the first polyester material is polyester modified by a hydrophilic substance, and the second polyester material is polyester not modified by the hydrophilic substance; the polymer microsphere has a through pore channel structure, the through pore channel structure forms pores in the surface of the polymer microsphere, and the interior of the polymer microsphere is of a porous honeycomb structure. When the polymer microsphere provided by the invention is used as an organism filling agent, the polymer microsphere has a good adhesion effect on cells, better cell adhesion can be realized, and collagen secretion is improved; meanwhile, the through pore channel structure enables body tissues to grow more uniformly in the microspheres and strengthens the combination of the tissues and the polymer microspheres, so that the polymer microspheres are not easy to displace after being injected and in the later degradation process, and the safety is improved.

The present patent application discloses a kind of recombinant collagen VII and its preparation method and use.The recombinant collagen VII is obtained by multiple repetitions of short amino acid sequence GLPGAKGEKGAP.The secretory yield of the recombinant collagen VII in commercialized pichia expression system can be as high as about 17.3g / L, the extracellular secreted protein is basically not degraded during fermentation process, and more than 90% purity can be achieved by simple ultrafiltration and decolorization, industrial production can be realized;Moreover, the recombinant collagen VII has more optimal biological activities of promoting autophagy, improving endoplasmic reticulum stress state, promoting collagen secretion and inhibiting matrix metalloproteinase degradation of collagen, and shows good firming and anti-wrinkle effects.

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The invention discloses a traditional Chinese medicine mouthwash composition for strengthening teeth and refreshing oral cavity, the traditional Chinese medicine mouthwash composition comprises traditional Chinese medicine active components and a mouthwash matrix, and the traditional Chinese medicine active components comprise, by weight, 500-900 parts of ginseng, 10-20 parts of prunella vulgaris, 10-20 parts of coptis chinensis and 10-30 parts of turmeric. The invention also discloses a preparation method of the traditional Chinese medicine mouthwash composition. According to the traditional Chinese medicine mouthwash composition disclosed by the invention, the inhibition rate of anaerobic bacteria in the oral cavity can reach 42.1%-55.3%, the proliferation rate of gingival fibroblasts can reach up to 140.5%, the secretion amount of type I collagen of the gingival fibroblasts is increased to 24.4 ng / mL, and the effects of strengthening teeth and refreshing the oral cavity are remarkably realized.

The invention discloses porous polycaprolactone microspheres for tissue filling and a preparation method of the porous polycaprolactone microspheres, and relates to the field of biomedical materials. The microsphere is composed of polycaprolactone, the diameter of the microsphere is 40-70 [mu] m, the dispersion coefficient of the microsphere is less than 5%, the porosity of the microsphere is 55-85%, and the surface of the microsphere is provided with a uniform open pore structure formed by tridecane. According to the preparation method, a microfluidic technology is adopted, a tridecane-containing polycaprolactone dichloromethane solution is taken as a dispersion phase, a polyvinyl alcohol aqueous solution is taken as a continuous phase, an oil-in-water emulsion is formed through microchannel shearing, and curing, washing and freeze-drying are performed to obtain the tridecane-containing polycaprolactone / polyvinyl alcohol composite material. The microspheres disclosed by the invention have excellent monodispersity and a porous structure, and can remarkably promote fibroblast proliferation and collagen secretion and induce moderate inflammatory response to activate tissue regeneration. The process is simple and controllable, and the obtained microspheres have injectability and a tissue repair promoting function and have application prospects in the field of soft tissue repair.

The application provides a recombinant collagen IV and application thereof, and relates to the technical field of protein engineering.Amino acid residue repeating unit with good biological activity and stability in human collagen IV is found in the application, and the repeating unit is repeated and used as a core to construct a recombinant collagen IV, and then a new human recombinant collagen IV with biological activity is obtained.The human recombinant collagen IV provided in the application is a small molecular weight protein, is easy to be absorbed by a human body, has good safety, can significantly promote secretion of self collagen, has the effects of promoting cell adhesion and promoting cell migration, and has a good application prospect in basement membrane repair and remodeling.The application provides a new functional ingredient for products for repairing skin, tightening skin, resisting aging, resisting wrinkles, moisturizing and the like.

The application provides a kind of recombinant human collagen XVII and its preparation method and application, it is related to genetic engineering technical field.The preparation method of recombinant human collagen XVII includes the following steps: selecting the amino acid sequence Xvii-rich of GPP of human collagen XVII triple helix region 1127 to 1149 position, then codon optimization of yeast expression system is carried out, and the recombinant human collagen XVII is prepared according to the sequence of target gene.The molecular weight of recombinant collagen XVII is reduced compared with human collagen XVII, is not easy to degrade, and is 100% homologous with the amino acid sequence of natural collagen XVII, has no immunogenicity;By electrophoresis detection, the proportion of the complete target protein of recombinant human collagen XVII secreted to the outside of host cell is more than 90%, has the advantages of high stability and not easy to degrade.

This invention provides steroidal saponins such as Paris polyphylla saponin A, Paris polyphylla saponin B, Xuefengshan saponin A, Xuefengshan saponin B, and Xuefengshan saponin C, their preparation methods, and their applications in the cosmetics and pharmaceutical fields. It belongs to the field of cosmetics and pharmaceutical technology. Paris polyphylla saponin VII, protodiospin, protodiospin, protodiospin, Xuefengshan saponin B, Xuefengshan saponin C, and methyl protosaponin Pb are prepared at extremely low concentrations (0.1%). m At concentrations of M, it exhibits activity in promoting collagen secretion in adult dermal fibroblasts (HDFa), with an increase in collagen secretion exceeding 50%; furthermore, at this concentration, it is not cytotoxic and can be used to prepare anti-aging drugs or skincare products.

The invention discloses a light-operated cross-linked intelligent gel dressing of ginseng exosome composite self-assembled micelle and application of the light-operated cross-linked intelligent gel dressing, and belongs to the technical field of biology. The intelligent ginseng exosome with cell penetrating peptide modification and endosome escape functions is constructed through an ultrasonic incubation method, and meanwhile, the light-controlled cross-linked intelligent gel dressing capable of forming gel in situ is developed by adopting a light cross-linking technology. According to the dressing, the angiogenesis promoting function of the intelligent ginseng exosome and the collagen secretion promoting characteristic of the asiaticoside-loaded micelle are creatively integrated, efficient cell uptake is achieved through a gridding protein mediated endocytosis pathway, and endosome escape is completed by utilizing an environmental response mechanism. The co-loading system constructed by the invention has dual functions of wound protection and tissue regeneration, and a novel external preparation solution with high efficiency and safety is provided for local treatment of diabetic foot ulcer.

This invention discloses a method for analyzing the anti-aging and skin whitening mechanisms of bergamot extract based on network pharmacology and experimental verification. Belonging to the field of computer-aided drug screening technology, this method uses network pharmacology to predict the core targets of the active ingredients in bergamot and screens the signaling pathways regulated by the bergamot extract. Through DPPH free radical scavenging experiments, mushroom tyrosinase inhibition experiments, B16F10 cell melanin production inhibition experiments, HSF cell type I collagen secretion promotion experiments, and a UVB-induced mouse skin pigmentation model, the method systematically verifies that bergamot extract has multiple effects, including significantly inhibiting melanin synthesis, scavenging free radicals, promoting collagen secretion, and improving skin pigmentation. This invention is the first to combine computational prediction with experimental verification, fully elucidating the whitening and anti-aging potential of bergamot extract and its underlying molecular mechanisms, providing a reliable method and basis for developing novel natural cosmetic raw materials.

The invention provides anti-wrinkle and anti-aging essence containing a rhodiola rosea extract and a preparation method of the anti-wrinkle and anti-aging essence, and relates to the technical field of daily necessities. The skin care product comprises the following components in percentage by mass: 2%-5% of a rhodiola rosea extract, 0.5%-1% of a porphyra yezoensis extract, 1%-2% of a transdermal enhancer, 3%-5% of glycerol, 3%-5% of butanediol, 0.5%-1% of hydroxyethyl cellulose, 0.2%-0.8% of glyceryl stearate, 0.1%-0.5% of phenoxyethanol and the balance of water. The rhodiola rosea extract and the porphyra yezoensis extract are specifically combined for the first time and have a synergistic effect, collagen secretion can be promoted, fine wrinkles can be improved, and good anti-wrinkle and anti-aging effects are achieved.

The present application relates to the technical field of food and cosmetic efficacy evaluation, and particularly relates to application of trimethylamine liver metabolite in evaluation of anti-aging ability of food. The present application firstly induces HSF and HaCaT cells by using trimethylamine liver metabolite to obtain an oxidative damage cell model; then extracts a mother liquor by using extracts of anti-aging functional food, dilutes the mother liquor after sterilization to obtain a diluted mother liquor; pretreats HSF and HaCaT cells by using the diluted mother liquor; after the pretreatment, discards the diluted mother liquor, washes HSF and HaCaT cells by using PBS, then adds a mixed solution of trimethylamine liver metabolite and complete culture medium to act on the cells, and finally evaluates the anti-aging effect of food by detecting cell toxicity, cell antioxidant enzyme activity, cell oxidative stress level and cell inflammatory factor and collagen secretion level, so that a more extensive anti-aging evaluation method is provided.

The invention discloses recombinant human XVII type collagen and application of the recombinant human XVII type collagen and ginsenoside in anti-aging, and belongs to the technical field of biology. The amino acid sequence of the recombinant human XVII type collagen disclosed by the invention is as shown in SEQ ID NO. 2. The recombinant human XVII type collagen provided by the invention or the recombinant human XVII type collagen compounded with ginsenoside Rg5 can synergistically relieve skin pressure, reduce skin pain, promote collagen secretion and improve skin elasticity, thereby achieving an anti-aging effect. Therefore, the recombinant XVII type collagen and the combination of the recombinant XVII type collagen and ginsenoside have better application prospects in the fields of cosmetics and medicines.

The application belongs to the technical field of biological medicine and medical cosmetology materials, and discloses a sodium alginate-based facial filler, which comprises 13-26% of sodium alginate-based material, 40-43% of polylactic acid microspheres and 34-44% of lyophilization protectant in percentage by mass; the sodium alginate-based material comprises sodium alginate and oxidized sodium alginate, the G / M ratio of the sodium alginate and the oxidized sodium alginate is 3:1-1:1, and the mass ratio of the oxidized sodium alginate to the sodium alginate is 2:1-1:2. The sodium alginate-based material with biodegradable stimulation characteristics is used, the aldehyde group of the oxidized sodium alginate can react with the amino group on the surface of the tissue to form a Schiff base, thereby helping to anchor the target site, and the sodium alginate-based material can act on cells together with the polylactic acid microspheres to promote collagen secretion, the facial filler also has the functions of timely filling and gradient degradation. The application also provides a preparation method of the sodium alginate-based facial filler.

The application belongs to the field of daily chemicals, and discloses a composition containing PDRN and having the effect of tightening and anti-wrinkle, wherein the composition comprises PDRN, an extract of Saururus chinensis, a root oil of Phlox subulata, a seed oil of Rosa rubus, and a seed oil of Panax ginseng. The composition provided by the application uses PDRN, the extract of Saururus chinensis, the root oil of Phlox subulata, the seed oil of Rosa rubus, and the seed oil of Panax ginseng in combination, and through mutual cooperation of the components, synergistically promotes the secretion of collagen type I, so that the composition has the effect of tightening and anti-wrinkle. In addition, the composition provided by the application also has the effect of barrier repair, can significantly down-regulate the TEWL value of skin, is mild and non-irritating, and is suitable for people with sensitive skin.