55 results about "Collagen secretion" patented technology

The invention provides recombinant IV type collagen and application thereof, and relates to the technical field of protein engineering. In the invention, an amino acid residue repetitive unit with good biological activity and stability in the human-derived IV-type collagen is found, and the amino acid residue repetitive unit is used as a core for repeating and constructing the recombinant IV-type collagen, so that the novel human-derived recombinant IV-type collagen with biological activity is obtained. The human-derived recombinant IV-type collagen provided by the invention is a small-molecular-weight protein, is easy to absorb by a human body, is good in safety, can remarkably promote secretion of collagen, also has the effects of promoting cell adhesion and cell migration, and has a good application prospect in the aspects of basement membrane repair and remodeling. A new functional component is provided for products for repairing and tightening the skin, resisting aging, resisting wrinkles, preserving moisture and the like.

The invention discloses an extracellular matrix fusion protein as well as a preparation method and application thereof, relates to the technical field of recombinant proteins, and aims to solve the technical problems that protein cosmetics in the market are relatively single in effect and difficult to meet application requirements. According to the extracellular matrix fusion protein, the nucleotide sequence of the extracellular matrix fusion protein is as shown in SEQ ID No.7. According to the extracellular matrix fusion protein with the nucleotide sequence shown as SEQ ID No.7, an in-vitro cell test proves that the fusion protein can remarkably promote cell adhesion, proliferation and cell migration, can act on a corium layer in a transdermal mode and remarkably promote collagen secretion, and due to the fact that recombinant protein belongs to biological protein, the fusion protein can be used for preparing the extracellular matrix fusion protein with the nucleotide sequence shown as SEQ ID No.7. Compared with common chemical cosmetics and hormone drugs, the composition has the advantages of easiness in decomposition, no residue and the like, and has a wider application prospect.

The invention discloses a recombinant VII type collagen as well as a preparation method and application thereof. The recombinant VII type collagen is obtained by repeating a short amino acid sequence GLPGAKGEKGAP for a plurality of times. The secretion yield of the recombinant VII type collagen in a commercial pichia pastoris expression system can be up to about 17.3 g / L, the extracellular secreted protein is basically not degraded in the fermentation process, the purity can reach 90% or above through simple ultrafiltration and decoloration, and industrial mass production can be achieved; and the recombinant VII type collagen has better biological activities of promoting autophagy, improving an endoplasmic reticulum stress state, promoting collagen secretion and inhibiting matrix metalloproteinase from degrading collagen, and shows good tightening and anti-wrinkle effects.

The invention relates to an anti-aging composition and application thereof. The anti-aging composition is prepared from the following raw materials: dendrobium officinale, camellia japonica and polygonatum kingianum. Dendrobium officinale, camellia japonica and polygonatum kingianum cooperate with one another to achieve a synergistic effect, secretion of type I collagen (COL-1) can be promoted, secretion of matrix metalloproteinase-1 (MMP-1) can be inhibited, and decomposition of collagen in extracellular matrixes can be slowed down. The anti-aging composition can increase skin elasticity, improve the skin barrier function, reduce the percutaneous water loss rate and delay skin aging.

The invention belongs to the field of daily chemicals, and discloses a PDRN-containing composition with firming and anti-wrinkle effects, and the composition comprises PDRN, saururus chinensis extract, vetiver root oil, rusty rose seed oil and ginseng seed oil. According to the composition provided by the invention, the PDRN, the saururus chinensis extract, the vetiver root oil, the rusty rose seed oil and the ginseng seed oil are compounded for use, and the components are mutually matched and synergistically promoted to obtain a synergistic effect in the aspect of promoting the secretion of type I collagen, so that the composition has remarkable tightening and anti-wrinkle effects. In addition, the composition provided by the invention also has a certain barrier repairing effect, can significantly reduce the TEWL value of skin, is mild and non-irritant, and is suitable for people with sensitive skin.

The invention discloses a skin tissue injury repairing composition containing an umbilical cord extract as well as a preparation method and application of the skin tissue injury repairing composition. The composition provided by the invention comprises the umbilical cord extract, the silk fibroin, the gamma-PGA and the antioxidant, has a synergistic effect by accurately controlling the component proportion, can promote cell proliferation and increase collagen secretion, and has a remarkable skin tissue injury repair effect; furthermore, an enzymolysis process of active ingredients of the composition is optimized, so that the yield and the purity of the umbilical cord extract are improved, and the repairing effect of the composition is also improved; meanwhile, by preparing the PLGA nano drug-loaded particles, the stability of the composition is improved, stable delivery and long-acting release of the drug are achieved, efficient transdermal absorption can be achieved, a better skin injury repairing effect is achieved, the advantages of being simple in preparation process, high in stability, remarkable in repairing effect and the like are achieved, and the PLGA nano drug-loaded particles can be used for preparing more efficient drugs; the ointment is suitable for treating various skin injuries such as burns, wounds, ulcers and the like.

The invention provides an anti-aging repairing composition containing algae and application of the anti-aging repairing composition, the anti-aging repairing composition comprises blue-green algae, tripeptide and a bacillus fermentation product, and the mass ratio of the blue-green algae to the tripeptide to the bacillus fermentation product is (0.01-0.5): (0.1-1): (0.2-2). The composition obtained by compounding the blue-green algae, the tripeptide and the bacillus fermentation product has a synergistic effect in the aspect of resisting senility, especially in the aspect of promoting the secretion amount of collagen, and when the blue-green algae is replaced with other algae or the bacillus fermentation product is replaced with other fungus fermentation products, the effect of resisting senility is achieved. The effects of the composition disclosed by the invention cannot be achieved. Meanwhile, the composition provided by the invention also has multiple effects of repairing a barrier, resisting oxidation, resisting inflammation, promoting intercellular interaction, promoting collagen synthesis, inhibiting collagen degradation and the like.

The invention discloses a preparation method of a three-dimensional umbilical cord mesenchymal stem cell exosome and application of the three-dimensional umbilical cord mesenchymal stem cell exosome in the fields of medicines and medical beauty. The preparation method comprises the step of amplifying umbilical cord mesenchymal stem cells (UC-MSCs) by adopting a three-dimensional culture system. Experiments show that IGF-1, EGF, VEGF, TGF-beta, PDGF, FGF and the like in the exosome subjected to three-dimensional culture are beneficial to skin repair and oxidation resistance, and the content of cell active factors for promoting collagen secretion is remarkably increased. The umbilical cord mesenchymal stem cell exosome significantly improves the fibroblast proliferation activity, and UV-induced skin fibroblast damage model construction and function verification experiments also prove that the exosome can promote the HA level recovery of damaged skin fibroblasts. The invention provides an efficient and safe biological material for tissue repair.

The invention discloses a recombinant III-type collagen and PDRN compound composition and a preparation method thereof, and relates to the technical field of biological medicines. The composition is formed by compounding recombinant human III type collagen and polydeoxyribonucleotide according to the mass ratio of 1: 10-10: 1, the weight-average molecular weight of the recombinant human III type collagen and the weight-average molecular weight of the polydeoxyribonucleotide are 50-100 kDa, the ratio can generate synergistic interaction, fibroblast proliferation, migration and collagen secretion are remarkably promoted, and the activity of the composition is superior to that of a single component and commercially available products. The composition can be used for preparing medical instruments, cosmetics or water-light injections for skin repair, rejuvenation or skin improvement. The invention also provides a water-light injection containing the compound composition, and the obtained preparation is stable, clear, transparent, safe and non-irritant.

This invention provides an anti-aging and repair dressing containing four complex active factors and its preparation method. The dressing comprises 75-90 parts of a matrix material, 5-15 parts of four complex active factors, and 3-8 parts of a moisturizing auxiliary agent. The four complex active factors are composed of recombinant human type III collagen, acetyl hexapeptide-8, ectoine, and dipotassium glycyrrhizate in a ratio of (3-6):(1-3):(2-4):(1-2). The matrix is ​​a hydrophilic nonwoven fabric or bio-cellulose membrane material that has undergone low-temperature plasma surface grafting activation treatment. The preparation process employs nitrogen combined with vacuum pulse freeze-drying technology, and enhances the activity stability through an ectoine complexation step. This dressing can promote HaCaT cell proliferation, increase collagen secretion, and inhibit inflammatory factors, significantly improving skin barrier function. It has good biocompatibility and can be used to prepare products that improve skin aging and repair.

This invention discloses recombinant type III collagen, its preparation method, and its applications. The amino acid sequence of the recombinant type III collagen is shown in SEQ ID NO: 9. The recombinant type III collagen integrates the RGDV highly active motif and the EKEK hydrophilic stable domain with the core structure of type III collagen to achieve an integrated design of "high activity and long-lasting effect". The in vitro cell migration and collagen secretion effects are improved by more than 50% compared with wild type. Through anti-enzyme cleavage site modification and hydrophilic modification, the in vivo residence half-life is significantly prolonged to meet the long-term repair needs of the dermis.

The present application provides gastrodia elata (Blume) alkaloids PFT201A, PFT201B and PFT203 and a preparation method thereof, and application thereof in the fields of cosmetics and medicine. Gastrodia elata Gastrodia elata (Blume) alkaloids PFT201A, PFT201B and PFT203 significantly promote the secretion of collagen type II in human dermal fibroblasts-adult (HDFa) at an extremely low concentration (0.1 μ M), and have no cytotoxicity at the concentration. These compounds can be used for preparing anti-aging drugs or skin care products.

This invention provides a method for obtaining ingredients from Dulong Paris polyphylla (… Paris dulongensis A method for preparing parisyunnanoside G (PFE55) in H. Li & Kurita and its application in cosmetics and pharmaceuticals. This research belongs to the field of cosmetics and pharmaceutical technology. PFE55 is used at extremely low concentrations (0.1... μ At a concentration of g / mL, it exhibits activity in promoting collagen secretion in adult dermal fibroblasts (HDFa) and is non-cytotoxic at this concentration, making it suitable for the preparation of anti-aging drugs or skincare products.

The present invention relates to the application of turmeric extracellular vesicles in the field of cosmetics. The present invention discovers for the first time that turmeric extracellular vesicles have anti-aging, repair, and antioxidant effects, and have good water solubility, excellent permeability, and are easily absorbed by the skin; they can (a) promote the proliferation of fibroblasts, (b) promote the collagen secretion of fibroblasts, (c) inhibit the level of MMP1 in fibroblasts, (d) promote the level of TMP1 in fibroblasts, (e) inhibit the level of ROS in fibroblasts, (f) inhibit the level of SA-β-Gal in fibroblasts, (g) promote the proliferation level of keratinocytes, (h) inhibit the level of ROS in keratinocytes, (i) promote the expression of keratinization-related proteins in keratinocytes, and (j) promote the migration level of keratinocytes.

This invention discloses a therapeutic molecule, nanoparticles, and their preparation method for locating and stabilizing atherosclerotic plaques. The therapeutic molecule is synthesized based on cholanonic acid and copper peptide, and the nanoparticles are formed by the self-assembly of the therapeutic molecule. The preparation method involves aldehyde modification of both ends of cholanonic acid and coupling it with copper peptide via a Schiff base reaction to obtain a therapeutic molecule with both diagnostic and therapeutic functions. The copper peptide structure in the therapeutic molecule serves as the hydrophilic segment, and the cholanonic acid structure serves as the hydrophilic segment, allowing it to further self-assemble into nanoparticles in water. These nanoparticles exhibit acid responsiveness and can disintegrate in the slightly acidic environment of atherosclerotic sites. The released copper peptide can stabilize plaques by promoting collagen secretion and inhibiting matrix metalloproteinase expression. Simultaneously, the X-ray imaging characteristics of cholanonic acid can be used to locate and diagnose the distribution of plaques, thus achieving integrated stabilization and localization of atherosclerotic plaques.

This invention belongs to the field of biomedical technology, specifically relating to the application of mesenchymal stem cells in the preparation of anti-aging drugs. The mesenchymal stem cells are human adipose-derived mesenchymal stem cells (hAMSCs) or mouse bone marrow mesenchymal stem cells (BM-MSCs). This invention utilizes mesenchymal stem cells to regulate senescent stromal cells and the spleen's ECM, improving the thinning stromal state in the tissue microenvironment through multiple targets. Specifically, it improves the aging-related secretory phenotype of stromal cells, enhances mitochondrial activity, strengthens their proliferation capacity, and delays the reduction of collagen secretion. This, in turn, delays the disordered distribution and functional decline of immune cells caused by aging and enhances tissue regeneration capacity, indirectly affecting various other cells in the tissue microenvironment, thus exhibiting an antagonistic effect against systemic organ aging.

The traditional Chinese medicine mouthwash comprises a traditional Chinese medicine compound and a mouthwash matrix, and the traditional Chinese medicine compound comprises the following components in parts by weight: 10-20 parts of golden cypress, 15-30 parts of angelica sinensis, 15-30 parts of honeysuckle, 15-30 parts of chrysanthemum and 15-30 parts of liquorice. The invention also provides a preparation method of the traditional Chinese medicine mouthwash for relieving gingivitis. The traditional Chinese medicine mouthwash for relieving gingivitis can promote proliferation of gingival fibroblasts, promote secretion of gingival fibroblast collagen and effectively inhibit proliferation of oral anaerobic bacteria, thereby relieving gingivitis.

The invention provides an application of active compounds PTM39A and PTM40 in pedicels and inflorescences of Gastrodia elata Blume in the preparation of medicines and cosmetics. The active compounds PTM39A and PTM40 in the pedicels and the inflorescences of Gastrodia elata Blume are used for preparing medicines and cosmetics. Belongs to the technical field of cosmetics and medicines. According to the present invention, the compound PTM39A and the compound PTM40 can significantly promote the secretion of the type I collagen in the adult dermal fibroblasts (human dermis fibroblasts, HDFa) at the extremely low concentration (1 [mu] g / mL), and have no cytotoxicity at the concentration, such that the compound PTM39A and the compound PTM40 can significantly promote the secretion of the type I collagen in the human dermis fibroblasts (HDFa), and can significantly promote the secretion of the type I collagen in the human dermis fibroblasts (HDFa), the compound can be used for preparing anti-aging medicines or skin care products.

The application relates to a recombinant type I collagen and application thereof, which utilizes the characteristics of Pichia pastoris capable of secreting and expressing recombinant proteins, secretes the recombinant type I collagen on the culture medium supernatant, and reduces the difficulty of protein purification. In addition, the integrin site RGD is inserted into the collagen to form a recombinant type I collagen with high stability and high expression. According to the relative proliferation of cells and the relative adhesion experiment of cells, it can be seen that the recombinant type I collagen has the effects of promoting the proliferation of skin fibroblasts, improving the skin, promoting wound healing, promoting the adhesion of fibroblasts in the skin layer and the like. Therefore, the recombinant type I collagen has great potential applications in the fields of skin care and beauty, medical treatment and the like.

The invention discloses application of a platelet exosome in preparation of a medicine for improving skin conditions. The invention further discloses the platelet exosome, a pharmaceutical composition containing the platelet exosome and application of the platelet exosome and the pharmaceutical composition in preparation of skin care products or cosmetics. The platelet exosome can promote human fibroblast proliferation, further promote collagen secretion, increase skin thickness and fade wrinkles. Meanwhile, cell senescence caused by UV damage can be reduced, and the proportion of senescent cells is greatly reduced. The blank of the platelet exosome in the aspect of delaying skin aging is filled, and the platelet exosome has a huge application prospect in medical and beauty industries.

The invention discloses application of recombinant human glaze matrix protein in skin repair dressing, and belongs to the technical field of biomedicine. The skin repair dressing comprises recombinant human glaze matrix protein, sodium hyaluronate, chondroitin sulfate, osa starch and propylgallate wrapped by lipidosome, and the repair effect is improved through a multiple synergistic mechanism. The preparation method of the dressing comprises the following steps: mixing the recombinant human glaze matrix protein with the stabilizer, and then carrying out homogeneous binding with the sodium hyaluronate, the osa starch, the liposome propyl gallate and the hydrogel matrix. Experiments prove that after the dressing is used, the wound healing speed is increased, the III type collagen secretion amount is increased, the TGF-beta1 expression is reduced, the scar index is reduced, and the dressing is suitable for burn and surgical incision repair.

The application discloses an extracellular matrix fusion protein and a preparation method and application thereof, relates to the technical field of recombinant proteins, and aims to solve the technical problem that the existing protein cosmetic products on the market have relatively single functions and are difficult to meet application requirements. The extracellular matrix fusion protein has a nucleotide sequence as shown in SEQ ID No. 7. The extracellular matrix fusion protein with the nucleotide sequence as shown in SEQ ID No. 7 can significantly promote cell adhesion and proliferation, promote cell migration, and act on the dermis layer through the skin, and significantly promote collagen secretion, and since the recombinant protein belongs to a biological protein, has the advantages of easy decomposition and no residue, and has a more broad application prospect compared with ordinary chemical cosmetic products and hormone drugs.

The invention provides recombinant human XVII type collagen as well as a preparation method and application thereof, and relates to the technical field of genetic engineering. The preparation method of the recombinant human XVII type collagen comprises the following steps: selecting an amino acid sequence XVII-rich of GPP from the 1127th site to the 1149th site of a triple helix region of the human XVII type collagen, then performing codon optimization of a yeast expression system, and preparing the recombinant human XVII type collagen according to a target gene sequence. Compared with human XVII type collagen, the recombinant XVII type collagen has the advantages that the molecular weight is reduced, the recombinant XVII type collagen is not easy to degrade, the amino acid sequence of the recombinant XVII type collagen is 100% homologous with that of natural XVII type collagen, and the recombinant XVII type collagen has no immunogenicity; through electrophoresis detection, the proportion of complete target protein secreted out of host cells by the recombinant human XVII type collagen is more than 90%, and the recombinant human XVII type collagen has the advantages of high stability and difficulty in degradation.

The invention relates to the technical field of protein engineering or genetic engineering, and particularly discloses a method for improving the secretory expression efficiency of recombinant III-type collagen (Col3) in pichia pastoris in a fermentation tank. The invention discloses pichia pastoris subjected to fermentation culture. The pichia pastoris simultaneously expresses a type III collagen gene, a secretion signal peptide gene and a gene expression regulation element. The pichia pastoris is subjected to four-stage fermentation culture (not traditional three-stage fermentation culture), high expression of recombinant III type collagen in a fermentation tank can be achieved, and the yield is at least 10 g / L or above. The technical scheme provided by the invention has the effect of greatly improving the fermentation yield of the recombinant III type collagen.

The invention provides a polymer microsphere as well as a preparation method and application thereof. The polymer microspheres contain a first polyester material and a second polyester material, the first polyester material is polyester modified by a hydrophilic substance, and the second polyester material is polyester not modified by the hydrophilic substance; the polymer microsphere has a through pore channel structure, the through pore channel structure forms pores in the surface of the polymer microsphere, and the interior of the polymer microsphere is of a porous honeycomb structure. When the polymer microsphere provided by the invention is used as an organism filling agent, the polymer microsphere has a good adhesion effect on cells, better cell adhesion can be realized, and collagen secretion is improved; meanwhile, the through pore channel structure enables body tissues to grow more uniformly in the microspheres and strengthens the combination of the tissues and the polymer microspheres, so that the polymer microspheres are not easy to displace after being injected and in the later degradation process, and the safety is improved.

The present patent application discloses a kind of recombinant collagen VII and its preparation method and use.The recombinant collagen VII is obtained by multiple repetitions of short amino acid sequence GLPGAKGEKGAP.The secretory yield of the recombinant collagen VII in commercialized pichia expression system can be as high as about 17.3g / L, the extracellular secreted protein is basically not degraded during fermentation process, and more than 90% purity can be achieved by simple ultrafiltration and decolorization, industrial production can be realized;Moreover, the recombinant collagen VII has more optimal biological activities of promoting autophagy, improving endoplasmic reticulum stress state, promoting collagen secretion and inhibiting matrix metalloproteinase degradation of collagen, and shows good firming and anti-wrinkle effects.

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The invention provides an extract of flowers and seeds of Mesua ferrea L. capable of promoting collagen activity as well as a preparation method and application of the extract. Belongs to the technical field of cosmetics and medicines. The preparation method comprises the following steps: respectively extracting dried flowers and seeds of the ferrocene by using a solvent to prepare MY135, MY125, MY126 and MY127; the ferrugina extracts MY135, MY125 and MY126 have the activity of promoting collagen secretion, can be used for preparing anti-aging drugs or skin care products, and provide new choices for application of the ferrugina extracts in drugs or cosmetics.

The invention discloses a traditional Chinese medicine mouthwash composition for strengthening teeth and refreshing oral cavity, the traditional Chinese medicine mouthwash composition comprises traditional Chinese medicine active components and a mouthwash matrix, and the traditional Chinese medicine active components comprise, by weight, 500-900 parts of ginseng, 10-20 parts of prunella vulgaris, 10-20 parts of coptis chinensis and 10-30 parts of turmeric. The invention also discloses a preparation method of the traditional Chinese medicine mouthwash composition. According to the traditional Chinese medicine mouthwash composition disclosed by the invention, the inhibition rate of anaerobic bacteria in the oral cavity can reach 42.1%-55.3%, the proliferation rate of gingival fibroblasts can reach up to 140.5%, the secretion amount of type I collagen of the gingival fibroblasts is increased to 24.4 ng / mL, and the effects of strengthening teeth and refreshing the oral cavity are remarkably realized.

The invention discloses porous polycaprolactone microspheres for tissue filling and a preparation method of the porous polycaprolactone microspheres, and relates to the field of biomedical materials. The microsphere is composed of polycaprolactone, the diameter of the microsphere is 40-70 [mu] m, the dispersion coefficient of the microsphere is less than 5%, the porosity of the microsphere is 55-85%, and the surface of the microsphere is provided with a uniform open pore structure formed by tridecane. According to the preparation method, a microfluidic technology is adopted, a tridecane-containing polycaprolactone dichloromethane solution is taken as a dispersion phase, a polyvinyl alcohol aqueous solution is taken as a continuous phase, an oil-in-water emulsion is formed through microchannel shearing, and curing, washing and freeze-drying are performed to obtain the tridecane-containing polycaprolactone / polyvinyl alcohol composite material. The microspheres disclosed by the invention have excellent monodispersity and a porous structure, and can remarkably promote fibroblast proliferation and collagen secretion and induce moderate inflammatory response to activate tissue regeneration. The process is simple and controllable, and the obtained microspheres have injectability and a tissue repair promoting function and have application prospects in the field of soft tissue repair.