Traditional Chinese medicine mouthwash for relieving gingivitis and preparation method thereof

Abstract

The traditional Chinese medicine mouthwash comprises a traditional Chinese medicine compound and a mouthwash matrix, and the traditional Chinese medicine compound comprises the following components in parts by weight: 10-20 parts of golden cypress, 15-30 parts of angelica sinensis, 15-30 parts of honeysuckle, 15-30 parts of chrysanthemum and 15-30 parts of liquorice. The invention also provides a preparation method of the traditional Chinese medicine mouthwash for relieving gingivitis. The traditional Chinese medicine mouthwash for relieving gingivitis can promote proliferation of gingival fibroblasts, promote secretion of gingival fibroblast collagen and effectively inhibit proliferation of oral anaerobic bacteria, thereby relieving gingivitis.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine mouthwash for relieving gingivitis and a preparation method thereof. BACKGROUND

[0002] Gingivitis is a common inflammation of the gums in the mouth, usually caused by the accumulation of plaque, a sticky film of bacteria, on the teeth. As plaque builds up on the teeth, the toxins released by the bacteria can irritate the gums, leading to redness, swelling, bleeding, and pain. Symptoms of gingivitis often include bleeding gums when brushing, bad breath, and swollen gums. Although gingivitis is reversible, and good oral hygiene practices such as proper brushing and flossing can usually restore health, if left untreated, gingivitis can progress to more serious periodontal disease.

[0003] The harm of gingivitis is not limited to local discomfort, but can also trigger a series of oral problems. Long-term untreated gingivitis can cause gradual damage to the periodontal tissue, leading to periodontitis, and eventually causing tooth loosening or even loss. In addition, the bacterial infection of gingivitis can also spread through the blood, causing systemic health problems such as heart disease, diabetes, and respiratory diseases. Therefore, gingivitis not only affects oral health, but also poses a potential threat to systemic health.

[0004] Gingival fibroblasts (GFs) play an important role in the occurrence and development of gingivitis. Gingival fibroblasts are the main cell type in the gingival tissue, mainly responsible for the structure and repair function of the gingival tissue. They maintain the elasticity and structural integrity of the gums by synthesizing and secreting extracellular matrix components such as collagen and elastin. Gingival fibroblasts not only bear structural functions in gingivitis, but also participate in immune responses. When bacterial infection in the oral cavity causes inflammation, gingival fibroblasts are activated by inflammatory factors such as cytokines and chemokines, promoting the persistence of inflammatory reactions. They secrete some pro-inflammatory factors such as IL-1β and TNF-α, exacerbating local inflammatory reactions, leading to symptoms such as gum pain, swelling, redness, and bleeding. Therefore, slowing down the inflammatory response of gingival fibroblasts is an important part of preventing and treating gingivitis.

[0005] Using non-steroidal anti-inflammatory drugs (NSAIDs) or glucocorticoids directly on the gums can inhibit the release of inflammatory factors such as prostaglandins, IL-6, and TNF-α, reducing redness and pain, however, this can only alleviate symptoms and cannot eradicate pathogenic bacteria or plaque, and is prone to recurrence after stopping medication. Long-term use of glucocorticoids can cause gingival atrophy, mucosal thinning, or secondary fungal infection, and NSAIDs can stimulate the gastrointestinal tract or cause allergies.

[0006] Traditional Chinese medicine compound is usually composed of multiple herbs, and each herb can exert anti-inflammatory effect through different pathways. Compared with western medicine, traditional Chinese medicine compound usually has less side effects and has unique advantages. Many western medicines may inhibit immune system or cause other adverse reactions, while traditional Chinese medicine compound generally improves local problems by adjusting the whole body environment, has less side effects and is relatively safe. SUMMARY

[0007] The purpose of the present application is to provide a traditional Chinese medicine capable of effectively relieving gingivitis in order to solve the above technical problems.

[0008] In order to achieve the above purpose, the present application provides a traditional Chinese medicine for relieving gingivitis, which comprises a traditional Chinese medicine compound and a mouthwash base, wherein the traditional Chinese medicine compound comprises, by weight, 10-20 parts of Cortex Phellodendri, 15-30 parts of Angelica sinensis, 15-30 parts of Lonicera japonica, 15-30 parts of Chrysanthemum morifolium and 15-30 parts of Glycyrrhiza uralensis. More preferably, the traditional Chinese medicine compound consists of, by weight, 10-20 parts of Cortex Phellodendri, 15-30 parts of Angelica sinensis, 15-30 parts of Lonicera japonica, 15-30 parts of Chrysanthemum morifolium and 15-30 parts of Glycyrrhiza uralensis.

[0009] Cortex Phellodendri (dried bark of Phellodendron amurense Rupr. Phellodendron chinense Schneid.) is cold in nature and bitter in taste, and is related to kidney and bladder meridians, and can clear heat and dry dampness, purge fire and detoxify; Lonicera japonica (Thunb.) is cold in nature and can dispel wind-heat, cool blood and detoxify; Chrysanthemum morifolium (dried capitulum of Chrysanthemum morifolium Ramat.) can clear liver and eyesight and dispel wind-heat, and the three can synergistically enhance the effect of clearing and resolving heat and toxin in upper jiao, and relieve gingival inflammation. Angelica sinensis (dried root of Angelica sinensis (Oliv.) Diels) can tonify blood and activate blood, and its warm nature can regulate the cold nature of Cortex Phellodendri and Lonicera japonica, so as to avoid over-coldness damaging stomach; at the same time, it can improve local blood circulation and promote inflammation dissipation. Glycyrrhiza uralensis (dried root and rhizome of Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L.) can regulate various drugs, clear heat and detoxify, relieve pain and protect the stomach from stimulation by cold drugs. The present traditional Chinese medicine compound is suitable for the following types of syndromes: stomach fire being in excess (gingival redness and swelling, bleeding and constipation) or wet heat invading upward (gingival pus, yellow and greasy tongue coating). Lonicera japonica Chrysanthemum morifolium Angelica sinensis Glycyrrhiza uralensis Glycyrrhiza inflata Glycyrrhiza glabra

[0010] As a preferred embodiment, the traditional Chinese medicine compound comprises, by weight, 15 parts of Cortex Phellodendri, 20 parts of Angelica sinensis, 20 parts of Lonicera japonica, 20 parts of Chrysanthemum morifolium and 20 parts of Glycyrrhiza uralensis. More preferably, the traditional Chinese medicine compound consists of, by weight, 15 parts of Cortex Phellodendri, 20 parts of Angelica sinensis, 20 parts of Lonicera japonica, 20 parts of Chrysanthemum morifolium and 20 parts of Glycyrrhiza uralensis. ​​​​​​

[0011] As a preferred embodiment, the weight ratio of the Chinese medicine compound component to the mouthwash base is 0.5-1.5:10.

[0012] As a preferred embodiment, the mouthwash base comprises a humectant, a preservative, a surfactant, a chelating agent, a conditioning agent, and water.

[0013] As a preferred embodiment, the mouthwash base consists of 6.5-19% humectant, 0.21-0.52% preservative, 0.2-0.5% surfactant, 0.01-0.05% chelating agent, 0.1-0.5% conditioning agent, and the balance of water, based on the total weight of the mouthwash base.

[0014] As a preferred embodiment, the humectant is any one or a combination of sorbitol, erythritol, or xylitol. More preferably, the humectant comprises 5-15% sorbitol, 1-3% erythritol, and 0.5-1% xylitol, based on the total weight of the mouthwash base.

[0015] As a preferred embodiment, the preservative is potassium sorbate, cetylpyridinium chloride, or a combination thereof, more preferably a combination of potassium sorbate and cetylpyridinium chloride, more preferably comprising 0.2-0.5% potassium sorbate and 0.01-0.02% cetylpyridinium chloride.

[0016] As a preferred embodiment, the surfactant is polysorbate-20.

[0017] As a preferred embodiment, the chelating agent is disodium EDTA.

[0018] As a preferred embodiment, the conditioning agent is dipotassium glycyrrhizinate.

[0019] In another aspect, the present application also provides a preparation method of the Chinese medicine mouthwash for relieving gingivitis, which comprises the following steps: (1) Quantitatively weighing each component of the Chinese medicine compound; (2) Adding ethanol with a concentration of 75% to 95% v / v to the mixture of Cortex Phellodendri, Angelica sinensis, Lonicera japonica, and Glycyrrhiza uralensis, and performing heating reflux extraction, then performing filtration separation to remove impurities, to obtain an extract, which is concentrated and dried at 60°C under reduced pressure to obtain an ethanol extract of Cortex Phellodendri, Angelica sinensis, Lonicera japonica, and Glycyrrhiza uralensis, and the weight-to-volume ratio of the mixture of Cortex Phellodendri, Angelica sinensis, Lonicera japonica, and Glycyrrhiza uralensis to ethanol is 1:8.

[0020] (3) Adding water to Chrysanthemum morifolium, performing heating reflux extraction, and then performing alcohol precipitation with ethanol with a concentration of 75% v / v to 95% v / v, then performing filtration separation to remove impurities, to obtain an extract, which is concentrated and dried at 60°C under reduced pressure to obtain a Chrysanthemum morifolium extract, and the weight-to-volume ratio of Chrysanthemum morifolium to water is 1:10. (4) mixing the ethanol extracts of Cortex Phellodendri, Angelica sinensis, Lonicera japonica and Glycyrrhiza uralensis with the extract of Chrysanthemum morifolium to obtain a traditional Chinese medicine compound; (5) mixing the traditional Chinese medicine compound and a mouthwash base, stirring uniformly to obtain the traditional Chinese medicine mouthwash.

[0021] Preferably, in the step (2), the concentration of ethanol is 75% v / v.

[0022] Preferably, in the step (3), the concentration of ethanol is 75% v / v.

[0023] Preferably, the reflux extraction temperature in each step is 100℃.

[0024] Preferably, the reflux extraction time in each step is 2 hours.

[0025] Preferably, the temperature of the reduced pressure drying is 60℃.

[0026] The traditional Chinese medicine mouthwash for relieving gingivitis of the present application can promote the proliferation of gingival fibroblasts, promote the secretion of collagen of gingival fibroblasts, effectively inhibit the proliferation of oral anaerobic bacteria, and thus relieve gingivitis. DETAILED DESCRIPTION

[0027] The present application will be further described below in conjunction with specific examples. It should be understood that the following examples are only used to illustrate the present application, and are not used to limit the scope of the present application.

[0028] Example 1 A traditional Chinese medicine mouthwash for relieving gingivitis, which is composed of a traditional Chinese medicine compound and a mouthwash base.

[0029] The components of the traditional Chinese medicine compound are as follows: Cortex Phellodendri 15g, Angelica sinensis 20g, Lonicera japonica 20g, Glycyrrhiza uralensis 20g, and Chrysanthemum morifolium 20g.

[0030] The components of the mouthwash base are as follows (by weight): Humectant: 5% sorbitol, 1% erythritol, 0.5% xylitol; Preservative: 0.2% potassium sorbate, 0.01% cetylpyridinium chloride; Surfactant: 0.2% polysorbate-20; Chelating agent: 0.01% disodium EDTA; Conditioning agent: 0.1% dipotassium glycyrrhizinate; the rest is supplemented with water to 100%.

[0031] The specific steps of the preparation method are as follows: (1) quantitatively weighing the components of the traditional Chinese medicine compound; (2) The cortex phellodendri, angelica, honeysuckle and licorice are added with 8 times weight multiple volume of 75% v / v concentration ethanol, and extracted by reflux extraction method, the reflux temperature is kept at 100°C, the extraction time is 2 hours, then the extraction liquid is separated by filtration to remove impurities, and the extraction liquid is concentrated and dried at 60°C under reduced pressure to obtain the ethanol extract of cortex phellodendri, angelica, honeysuckle and licorice.

[0032] (3) The chrysanthemum is added with 10 times weight multiple volume of softened water, and extracted by heating reflux, the reflux temperature is also 100°C, the extraction time is 2 hours, the extraction liquid is alcohol precipitated by 75% v / v concentration ethanol, then the extraction liquid is separated by filtration to remove impurities, and the extraction liquid is concentrated and dried at 60°C under reduced pressure to obtain the chrysanthemum extract.

[0033] (4) The ethanol extract of cortex phellodendri, angelica, honeysuckle and licorice is mixed with the chrysanthemum extract to obtain the traditional Chinese medicine compound.

[0034] (5) The traditional Chinese medicine compound is added into the mouthwash base (weight ratio 1:10), and stirred uniformly to prepare the traditional Chinese medicine mouthwash for relieving gingivitis.

[0035] Example 2 A traditional Chinese medicine mouthwash for relieving gingivitis is composed of a traditional Chinese medicine compound and a mouthwash base.

[0036] The components of the traditional Chinese medicine compound are as follows: cortex phellodendri 10g, angelica 15g, honeysuckle 15g, licorice 15g, and chrysanthemum 15g.

[0037] The components of the mouthwash base are as follows (by weight): Humectant: sorbitol (10%), erythritol (2%), and xylitol (1%); Preservative: 0.3% potassium sorbate and 0.02% cetylpyridinium chloride; Surfactant: 0.3% polysorbate-20; Chelating agent: 0.03% disodium EDTA; Conditioning agent: 0.2% dipotassium glycyrrhizinate; and the rest is supplemented with water to 100%.

[0038] The specific steps of the preparation method are as follows: (1) The traditional Chinese medicine compound components are weighed quantitatively; (2) The cortex phellodendri, angelica, honeysuckle and licorice are added with 8 times weight multiple volume of 80% v / v concentration ethanol, and extracted by reflux extraction method, the reflux temperature is kept at 100°C, the extraction time is 2 hours, then the extraction liquid is separated by filtration to remove impurities, and the extraction liquid is concentrated and dried at 60°C under reduced pressure to obtain the ethanol extract of cortex phellodendri, angelica, honeysuckle and licorice.

[0039] (3) The chrysanthemum is added to 10 times the weight multiple volume of soft water, heated to reflux extraction, the reflux temperature is also 100°C, the extraction time is 2 hours, the extract is alcohol precipitated by 85% v / v ethanol, then filtered and separated to remove impurities, the extract is concentrated and dried at 60°C under reduced pressure to obtain chrysanthemum extract.

[0040] (4) The ethanol extract of cortex phellodendri, angelica sinensis, honeysuckle and licorice is mixed with the chrysanthemum extract to obtain a traditional Chinese medicine compound.

[0041] (5) The traditional Chinese medicine compound is added to the mouthwash base (weight ratio 1.5:10), stirred uniformly, and a traditional Chinese medicine mouthwash for relieving gingivitis is prepared.

[0042] Example 3 A traditional Chinese medicine mouthwash for relieving gingivitis is composed of a traditional Chinese medicine compound and a mouthwash base.

[0043] The components of the traditional Chinese medicine compound are as follows: cortex phellodendri 20g, angelica sinensis 30g, honeysuckle 30g, and licorice 30g.

[0044] The components of the mouthwash base are as follows (by weight): Humectant: sorbitol (15%), erythritol (3%), and xylitol (1%); Preservative: 0.5% potassium sorbate and 0.02% cetylpyridinium chloride; Surfactant: 0.5% polysorbate-20; Chelating agent: 0.05% disodium EDTA; Conditioning agent: 0.5% dipotassium glycyrrhizinate, and the rest is supplemented with water to 100%.

[0045] The specific steps of the preparation method are as follows: (1) The components of the traditional Chinese medicine compound are weighed quantitatively; (2) 8 times the weight multiple volume of 95% v / v ethanol is added to cortex phellodendri, angelica sinensis, honeysuckle, and licorice, and reflux extraction is performed, the reflux temperature is maintained at 100°C, the extraction time is 2 hours, then filtration and separation are performed to remove impurities, the extract is concentrated and dried at 60°C under reduced pressure to obtain the ethanol extract of cortex phellodendri, angelica sinensis, honeysuckle, and licorice.

[0046] (3) The chrysanthemum is added to 10 times the weight multiple volume of soft water, heated to reflux extraction, the reflux temperature is also 100°C, the extraction time is 2 hours, the extract is alcohol precipitated by 95% v / v ethanol, then filtered and separated to remove impurities, the extract is concentrated and dried at 60°C under reduced pressure to obtain chrysanthemum extract.

[0047] (4) mixing the ethanol extract of Cortex Phellodendri, Angelica sinensis, Lonicera japonica and Glycyrrhiza uralensis with the extract of Chrysanthemum morifolium to obtain a traditional Chinese medicine compound.

[0048] (5) adding the traditional Chinese medicine compound into a mouthwash base (weight ratio 0.5:10), stirring uniformly to prepare a traditional Chinese medicine mouthwash for relieving gingivitis.

[0049] Comparative Example 1 A traditional Chinese medicine mouthwash is composed of a traditional Chinese medicine compound and a mouthwash base.

[0050] The components of the traditional Chinese medicine compound are as follows: Angelica sinensis 20 g, Lonicera japonica 20 g, Glycyrrhiza uralensis 20 g, and Chrysanthemum morifolium 20 g.

[0051] The components of the mouthwash base are as follows: Humectant: 5% sorbitol, 1% erythritol, 0.5% xylitol; Preservative: 0.2% potassium sorbate, 0.01% cetylpyridinium chloride; Surfactant: 0.2% polysorbate-20; Chelating agent: 0.01% disodium EDTA; Conditioning agent: 0.1% dipotassium glycyrrhizinate; the rest is supplemented with water to 100%.

[0052] The specific steps of the preparation method are as follows: (1) weighing the components of the traditional Chinese medicine compound; (2) adding 8 times the weight multiple volume of ethanol with a concentration of 75% v / v to Angelica sinensis, Lonicera japonica, and Glycyrrhiza uralensis, and extracting by reflux extraction, with the reflux temperature maintained at 100°C and the extraction time being 2 hours, then filtering and separating to remove impurities, obtaining the extract, and drying the extract by concentration under reduced pressure at 60°C to obtain the ethanol extract of Angelica sinensis, Lonicera japonica, and Glycyrrhiza uralensis.

[0053] (3) adding Chrysanthemum morifolium to 10 times the weight multiple volume of softened water, and extracting by heating reflux, with the reflux temperature being 100°C and the extraction time being 2 hours, then alcohol precipitation of the extract by 75% v / v ethanol, and filtering and separating to remove impurities, obtaining the extract, and drying the extract by concentration under reduced pressure at 60°C to obtain the extract of Chrysanthemum morifolium.

[0054] (4) mixing the ethanol extract of Angelica sinensis, Lonicera japonica, and Glycyrrhiza uralensis with the extract of Chrysanthemum morifolium to obtain a traditional Chinese medicine compound.

[0055] (5) adding the traditional Chinese medicine compound into a mouthwash base (weight ratio 1:10), stirring uniformly to prepare a traditional Chinese medicine mouthwash.

[0056] Comparative Example 2 A traditional Chinese medicine mouthwash is composed of a traditional Chinese medicine compound and a mouthwash base.

[0057] The components of the traditional Chinese medicine compound are as follows: 15 g of Huangbai, 20 g of Longxu, 20 g of Gancao, and 20 g of Juhua.

[0058] The components of the mouthwash base are as follows: Humectant: 5% sorbitol, 1% erythritol, 0.5% xylitol; Preservative: 0.2% potassium sorbate, 0.01% cetylpyridinium chloride; Surfactant: 0.2% polysorbate-20; Chelating agent: 0.01% disodium EDTA; Conditioning agent: 0.1% dipotassium glycyrrhizinate; the rest is supplemented with water to 100%.

[0059] The specific steps of the preparation method are as follows: (1) Quantitatively weigh the components of the traditional Chinese medicine compound; (2) Add 8 times the weight multiple volume of 75% v / v concentration ethanol to Huangbai, Longxu, and Gancao, and perform extraction by reflux extraction, with the reflux temperature kept at 100°C and the extraction time being 2 hours, then perform filtration separation to remove impurities, obtain the extraction liquid, and perform concentration and drying at 60°C under reduced pressure to obtain the ethanol extract of Huangbai, Longxu, and Gancao.

[0060] (3) Add 10 times the weight multiple volume of softened water to Juhua, perform heating reflux extraction with the reflux temperature being 100°C and the extraction time being 2 hours, perform alcohol precipitation on the extraction liquid by using 75% v / v concentration ethanol, then perform filtration separation to remove impurities, obtain the extraction liquid, and perform concentration and drying at 60°C under reduced pressure to obtain the Juhua extract.

[0061] (4) Mix the ethanol extract of Huangbai, Longxu, and Gancao with the Juhua extract to obtain the traditional Chinese medicine compound.

[0062] (5) Add the traditional Chinese medicine compound to the mouthwash base (weight ratio 1:10), stir uniformly, and prepare the traditional Chinese medicine mouthwash.

[0063] Comparative Example 3 A traditional Chinese medicine mouthwash is composed of a traditional Chinese medicine compound and a mouthwash base.

[0064] The components of the traditional Chinese medicine compound are as follows: 15 g of Huangbai, 20 g of Danggui, 20 g of Gancao, and 20 g of Juhua.

[0065] The components of the mouthwash base are as follows: Humectant: 5% sorbitol, 1% erythritol, 0.5% xylitol; Preservative: 0.2% potassium sorbate, 0.01% cetylpyridinium chloride; Surfactant: 0.2% polysorbate-20; Chelating agent: 0.01% disodium EDTA; Conditioning agent: 0.1% dipotassium glycyrrhizate; the rest is made up to 100% with water.

[0066] The specific steps of the preparation method are as follows: (1) Quantitatively weigh the ingredients of the traditional Chinese medicine compound; (2) Add 8 times the weight multiple volume of 75% v / v concentration ethanol to the cortex phellodendri, angelica and licorice, and extract by reflux extraction method, with the reflux temperature kept at 100°C and the extraction time of 2 hours, then filter and separate to remove impurities, obtain the extract, and concentrate and dry at 60°C under reduced pressure to obtain the ethanol extract of cortex phellodendri, angelica and licorice.

[0067] (3) Add the chrysanthemum to 10 times the weight multiple volume of softened water, heat and extract by reflux, with the reflux temperature also being 100°C and the extraction time being 2 hours, then alcoholize the extract with 75% v / v concentration ethanol, filter and separate to remove impurities, obtain the extract, and concentrate and dry at 60°C under reduced pressure to obtain the chrysanthemum extract.

[0068] (4) Mix the ethanol extract of cortex phellodendri, angelica and licorice with the chrysanthemum extract to obtain the traditional Chinese medicine compound.

[0069] (5) Add the traditional Chinese medicine compound to the mouthwash base (weight ratio 1:10), stir uniformly, and prepare the traditional Chinese medicine mouthwash.

[0070] Comparative Example 4 A traditional Chinese medicine mouthwash composed of a traditional Chinese medicine compound and a mouthwash base.

[0071] The components of the traditional Chinese medicine compound are as follows: cortex phellodendri 15g, angelica 20g, honeysuckle 20g, and licorice 20g.

[0072] The components of the mouthwash base are as follows: Humectant: 5% sorbitol, 1% erythritol, 0.5% xylitol; Preservative: 0.2% potassium sorbate, 0.01% cetylpyridinium chloride; Surfactant: 0.2% polysorbate-20; Chelating agent: 0.01% disodium EDTA; Conditioning agent: 0.1% dipotassium glycyrrhizate; the rest is made up to 100% with water.

[0073] The specific steps of the preparation method are as follows: (1) Quantitatively weigh the ingredients of the traditional Chinese medicine compound; (2) Add 8 times the weight of 75% v / v ethanol to Phellodendron bark, Angelica sinensis, Lonicera japonica and Glycyrrhiza uralensis, and extract by reflux extraction. The reflux temperature is maintained at 100℃ and the extraction time is 2 hours. Then filter and separate to remove impurities and obtain the extract. Concentrate and dry under reduced pressure at 60℃ to obtain ethanol extracts of Phellodendron bark, Angelica sinensis, Lonicera japonica and Glycyrrhiza uralensis.

[0074] (3) Add the ethanol extracts of Phellodendron bark, Angelica sinensis, Lonicera japonica and Glycyrrhiza uralensis to the mouthwash base (weight ratio 1:10), stir evenly, and prepare a Chinese medicine mouthwash to relieve gingivitis.

[0075] Efficacy Experiment The efficacy of the mouthwashes in Examples 1-3 and Comparative Examples 1-4 was tested through experiments.

[0076] Positive control: Dexamethasone Modeling reagent: Bacterial lipopolysaccharide (LPS) HGF cells (human gingival fibroblasts) were cultured in DMEM / F12 medium containing 10% FBS and 1% penicillin antibiotics (37°C, 5% CO2), with medium changes every other day, and passaged when 80% confluence was achieved. HGF cells were seeded into 6-well plates (1×10⁶ cells / wells). 5 ( / well), after cell adhesion, the cells were pretreated with serum-free medium for 6 hours. Except for the control group, the other groups were stimulated with LPS (1 μg / mL) for 1 hour, and then treated with Examples 1-3 and Comparative Examples 1-4 or dexamethasone for 24 hours respectively. The cell supernatant was collected, and the concentrations of IL-6, IL-1β, and TNF-α were detected according to the kit instructions.

[0077] Table 1. Effects of each comparative example on IL-6 expression in HGFs cells (unit: pg / mL)

[0078] Table 2. Effects of each comparative example on IL-1β expression in HGFs cells (unit: ng / mL)

[0079] Table 3. Effects of each comparative example on TNF-α expression in HGFs cells (unit: ng / mL)

[0080] As can be seen from the above results, the components of the mouthwash in Examples 1-3 of the present invention have a synergistic effect, have an anti-inflammatory effect, and can effectively relieve gingivitis.

[0081] The above merely describes the preferred embodiments of the present application, and it should be pointed out that, for those skilled in the art, several improvements and refinements can be made without departing from the principles of the present application, and these improvements and refinements should also be considered as falling within the protection scope of the present application.

Claims

1. A traditional Chinese medicine mouthwash for relieving gingivitis, comprising a traditional Chinese medicine compound and a mouthwash base, characterized in that, The traditional Chinese medicine compound, by weight, includes 10-20 parts of Phellodendron bark, 15-30 parts of Angelica sinensis, 15-30 parts of Lonicera japonica, 15-30 parts of Chrysanthemum morifolium, and 15-30 parts of Glycyrrhiza uralensis.

2. The herbal mouthwash according to claim 1, characterized in that, The herbal compound, by weight, includes 15 parts of Phellodendron bark, 20 parts of Angelica sinensis, 20 parts of Lonicera japonica, 20 parts of Chrysanthemum morifolium, and 20 parts of Glycyrrhiza uralensis.

3. The herbal mouthwash according to claim 1 or 2, characterized in that, The mouthwash base includes humectants, preservatives, surfactants, chelating agents, conditioning agents, and water.

4. The herbal mouthwash according to claim 3, characterized in that, The mouthwash base, by its total weight, consists of 6.5-19% humectant, 0.21-0.52% preservative, 0.2-0.5% surfactant, 0.01-0.05% chelating agent, 0.1-0.5% conditioning agent, and the balance being water.

5. The herbal mouthwash according to claim 3, characterized in that, The humectant is any one or a combination of sorbitol, erythritol, or xylitol.

6. The herbal mouthwash according to claim 3, characterized in that, The preservative is potassium sorbate, cetylpyridinium chloride, or a combination thereof.

7. The method for preparing the herbal mouthwash according to any one of claims 1 to 6, characterized in that... Includes the following steps: (1) Weigh each component of the traditional Chinese medicine compound in quantitative terms; (2) Add ethanol with a concentration of 75% to 95% v / v to the mixture of Phellodendron bark, Angelica sinensis, Lonicera japonica and Glycyrrhiza uralensis, heat and reflux to extract, then filter to separate and remove impurities to obtain the extract, concentrate and dry under reduced pressure at 60°C to obtain ethanol extract of Phellodendron bark, Angelica sinensis, Lonicera japonica and Glycyrrhiza uralensis, wherein the weight-volume ratio of the mixture of Phellodendron bark, Angelica sinensis, Lonicera japonica and Glycyrrhiza uralensis to ethanol is 1:

8. (3) Add water to chrysanthemum and heat and reflux to extract. The extract is precipitated with ethanol at a concentration of 75% v / v to 95% v / v, then filtered to remove impurities and obtain the extract. The extract is concentrated and dried under reduced pressure at 60°C to obtain chrysanthemum extract. The weight-volume ratio of chrysanthemum to water is 1:

10. (4) The ethanol extracts of the above-mentioned Phellodendron bark, Angelica sinensis, Lonicera japonica and Glycyrrhiza uralensis are mixed with the Chrysanthemum extract to obtain a traditional Chinese medicine compound; (5) Mix the Chinese herbal compound and the mouthwash base, stir evenly, and obtain the Chinese herbal mouthwash.

8. The method according to claim 7, characterized in that, The reflux extraction temperature in each step was 100℃.

9. The method according to claim 7, characterized in that, The reflux extraction time in each step is 2 hours.

10. The method according to claim 7, characterized in that, The temperature for vacuum drying is 60℃.

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