28 results about "Gingival fibroblast" patented technology

The invention provides a tantalum coating dental implant which is of an integrated structure and comprises an upper part, a middle part and a lower part, neck threads are arranged on the circumferential surface of the upper part, trapezoidal threads are arranged on the circumferential surfaces of the middle part and the lower part, and a self-tapping groove is further formed in the circumferential surface of the lower part; a thread-shaped surface groove is also formed in the trapezoidal thread; a hexagonal hole is formed in the top of the dental implant, a threaded hole is further formed in the bottom of the hexagonal hole, and the hexagonal hole and the threaded hole form an inner cavity stepped hole. The tantalum coating is applied to the surface of the titanium alloy, so that the mechanical strength of a matrix is guaranteed, physical shielding is formed, and release of toxic elements is prevented; the tantalum coating significantly improves the antibacterial performance and osteogenic activity of the titanium implant, and can promote adhesion, vitality, proliferation and migration of human gingival fibroblasts.

The invention relates to a composition with bacteriostatic, anti-inflammatory and / or oral cavity repairing effects and application of the composition in toothpaste, and belongs to the technical field of daily chemicals. The composition disclosed by the invention is prepared from the following components in parts by weight: 0.04 to 0.06 part of pyrroloquinoline quinone and 0.2 to 0.3 part of carboxymethyl chitosan. Based on the anti-oxidative stress regulation and control capability of pyrroloquinoline quinone and the broad-spectrum antibacterial synergistic effect of carboxymethyl chitosan, pyrroloquinoline quinone and carboxymethyl chitosan are compounded and applied to a toothpaste formula system, so that the toothpaste has the effects of inhibiting bacteria, resisting inflammation, proliferating and repairing gingival fibroblasts and regulating the composition and diversity of oral microorganisms. According to the composition disclosed by the invention, through a multi-mechanism synergistic effect, an oral health maintenance system integrating bacteriostatic protection, inflammation regulation and control and mucous membrane repair is constructed, and the compound requirements of modern consumers on oral microenvironment regulation and control, mucous membrane barrier strengthening and long-term health management are accurately met.

The invention discloses application of fermented lactobacillus mucus in preparation of a product for improving tooth color and gingivitis, and relates to the technical field of microorganisms. The lactobacillus fermentum NHNK-623 disclosed by the invention is preserved in the China Center for Type Culture Collection on November 18, 2024, and the preservation number of the lactobacillus fermentum NHNK-623 is CCTCC (China Center for Type Culture Collection) NO: M 20242574. The NHNK-623 has the effects of inhibiting proliferation and biofilm formation of pigment-generating pathogenic bacteria, agglutinating the pigment-generating pathogenic bacteria, inhibiting melanin generation of the pigment-generating pathogenic bacteria, inhibiting hyaluronidase activity of the pathogenic bacteria, promoting growth of gingival fibroblasts, inhibiting gingival cell inflammation and apoptosis induced by the pigment-generating pathogenic bacteria, and inhibiting melanin generation of the pigment-generating pathogenic bacteria. And the product can be used for preparing products for improving tooth color and gingivitis.

The invention belongs to the technical field of biomedicine, and particularly provides a periodontitis related marker which comprises glycochenodeoxycholic acid. The concentration change of the periodontitis related marker provided by the invention is directly related to the pathological mechanism of periodontitis. As bound bile acid, the bile acid is slowly degraded in saliva. And the glycochenodeoxycholic acid in the saliva is synthesized from gingival fibroblasts, so that the influence of systemic inflammatory diseases is greatly eliminated. Glycine chenodeoxycholic acid in saliva is used as a specific biomarker, a non-invasive, high-sensitivity and high-specificity detection method, system and equipment are established, objective quantitative evaluation and dynamic monitoring of periodontitis activity are achieved, the hysteresis limitation of traditional diagnosis is broken through, a convenient and non-invasive accurate diagnosis tool is provided for clinic, and the clinical application prospect is wide. And the technical blank of periodontitis activity noninvasive diagnosis is filled.

A composition is provided comprising a cell population and pharmaceuticals derived from human gingival fibroblasts, present in proportions not found in natural gingival tissue, but having a cellular phenotype preferentially selected to express proteins favorable for angiogenesis and anti-inflammatory effects, while reducing the formation of cell populations that promote tumorigenesis and / or metalloproteinases that inhibit tissue regeneration. A method for producing such a composition that increases proliferation several times over compared to previously known methods is provided, and a method for using such a composition in a wide range of human cell therapies is also provided.

This invention relates to the field of oral care products technology, specifically to an artificial enzyme with antibacterial properties, its preparation method, and its application. This technical solution employs a chemical reduction method, where a palladium source reacts in an environment containing surfactants and reducing agents to obtain palladium nanozymes. The obtained palladium nanozymes exhibit uniform morphology, controllable particle size, good dispersibility in water, and good biocompatibility; they have no significant toxic side effects on human gingival fibroblasts, possess highly efficient catalase activity, and demonstrate strong antibacterial and bactericidal effects against oral pathogens, significantly reducing bacterial drug resistance; they can inhibit acid production by oral bacteria, preventing tooth decay; and they retain their original catalytic activity even after multiple cycles of use. Applying the palladium nanozymes of this solution to the manufacture of oral care products can solve the technical problems of unsatisfactory safety and easy development of drug resistance in existing oral care products using antibacterial and anti-caries methods, showing promising application prospects.

The invention relates to the technical field of marine microorganisms, in particular to application of lactobacillus salivarius NHNK-603 in preparation of products for improving tooth color and gingivitis. The strain provided by the invention is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2024416. Experiments show; the NHNK-603 has the effects of inhibiting proliferation and biofilm formation of pigment-generating pathogenic bacteria, agglutinating the pigment-generating pathogenic bacteria, inhibiting melanin generation of the pigment-generating pathogenic bacteria, inhibiting hyaluronidase activity of the pathogenic bacteria, promoting growth of gingival fibroblasts, inhibiting gingival cell inflammation and apoptosis induced by the pigment-generating pathogenic bacteria, and inhibiting melanin generation of the pigment-generating pathogenic bacteria. And the product can be used for preparing products for improving tooth color and gingivitis.

The traditional Chinese medicine mouthwash comprises a traditional Chinese medicine compound and a mouthwash matrix, and the traditional Chinese medicine compound comprises the following components in parts by weight: 10-20 parts of golden cypress, 15-30 parts of angelica sinensis, 15-30 parts of honeysuckle, 15-30 parts of chrysanthemum and 15-30 parts of liquorice. The invention also provides a preparation method of the traditional Chinese medicine mouthwash for relieving gingivitis. The traditional Chinese medicine mouthwash for relieving gingivitis can promote proliferation of gingival fibroblasts, promote secretion of gingival fibroblast collagen and effectively inhibit proliferation of oral anaerobic bacteria, thereby relieving gingivitis.

The present invention provides a composition of matter comprising a population of cells and an agent, which is derived from human gingival fibroblasts and has a cell phenotype present in a proportion that is not found in the natural gingival tissue, the cell phenotype being preferentially selected to express a protein beneficial to angiogenesis and anti-inflammatory action, meanwhile, cell populations of metalloproteinase formation that promotes tumorigenicity and / or inhibits tissue regeneration are reduced. The present invention provides methods of producing the compositions that can achieve multiple proliferation improvements compared to previously known methods, and methods of using the compositions in a wide range of human cell therapies.

The invention discloses an exosome and drug loaded gradient aperture periodontal regeneration composite membrane and a preparation method thereof, and belongs to the field of biomedical materials. According to the composite membrane, RGD peptide modified freeze-dried chitosan is adopted as an inner-layer macroporous membrane, silk fibroin loaded with periodontal ligament stem cell source exosome is adopted as a middle-layer mesoporous membrane, silk fibroin loaded with medicine is adopted as an outer-layer microporous membrane, and a three-layer gradient aperture structure is constructed through an electrostatic spinning and freeze-drying combined technology. The interlayer bonding strength is improved through interface spraying of polyvinyl alcohol, the biological activity is guaranteed through an exosome freeze-drying protection technology, and time sequence synergistic release of rapid anti-inflammation of drugs and long-acting regeneration promotion of exosomes is achieved. The composite membrane has excellent mechanical property, degradation property and biological activity, can remarkably promote gingival fibroblast COL-I secretion and alveolar bone regeneration, and is applied to periodontitis tissue regeneration and repair.

The invention relates to a toothpaste composition, in particular to a synergistic toothpaste composition containing lipidosome vitamin C and ascorbyl palmitate, which remarkably enhances the effects of improving gingival health and repairing oral cavity through the synergistic effect of lipidosome VC and L-AP. The toothpaste comprises the following components in percentage by mass: 45%-60% of a humectant, 10%-20% of a friction agent, 2%-3% of a cleaning agent, 0.5%-1.5% of an adhesive, 0.1%-0.2% of a sweetening agent, 0.1%-0.2% of a preservative, 0.5%-1.5% of L-ascorbyl palmitate, 1%-3% of liposome vitamin C, 0.3%-0.7% of vitamin E, 5%-10% of vitamin B, 2%-5% of zinc gluconate and the balance of water. Cell experiments and clinical data prove that the composition can remarkably promote gingival fibroblast proliferation, enhance I-type collagen expression and effectively improve the problems of gingival bleeding, swelling and pain, oral ulcer and the like.

This invention belongs to the field of guided bone regeneration materials technology, specifically relating to an arginine carbon dot photoinitiated hydrogel prepolymer, its preparation method, and its application. The arginine carbon dot photoinitiated hydrogel prepolymer of this invention, when used for alveolar bone regeneration, inhibits the proliferation / migration of gingival fibroblasts (hGFs) while simultaneously inducing and promoting the proliferation / migration and osteogenic differentiation of bone marrow mesenchymal stem cells (hBMSCs), thus creating a "reverse-response" cell-selective regulatory effect.

The application discloses a Streptococcus salivarius CCFM1512 which can inhibit excessive inflammatory response and relieve periodontitis, and belongs to the technical field of microorganisms. The fermentation supernatant of the Streptococcus salivarius provided by the application can relieve inflammation of human gingival fibroblasts induced by Porphyromonas gingivalis lipopolysaccharide in vitro, and can significantly reduce the expression of inflammatory factors such as IL-1beta, IL-6 and IL-17A in gingival tissues, regulate the M1 / M2 polarization balance of macrophages and restore the expression level of DEL-1, so as to relieve alveolar bone absorption of rats. The Streptococcus salivarius has a great application prospect in the aspects of regulating oral inflammatory response and preparing products for relieving periodontitis.

Compositions of matter, including cell populations and medicaments, are provided that are derived from canine gingival fibroblasts and have cell phenotypes that occur in proportions not found in natural gum tissue, but rather, are preferentially are selected to express proteins favoring angiogenesis and anti-inflammatory effects, while reducing cell populations that promote tumorigenicity and / or formation of metalloproteinases that inhibit tissue regeneration. Methods of generating such compositions are provided that increase proliferation many-fold compared to previously known methods, and methods of using such compositions in a wide range of canine cell therapies are provided.

The invention relates to the technical field of biology, in particular to lactobacillus paracasei and application of the lactobacillus paracasei to improvement of tooth color and gingivitis. The strain provided by the invention is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20251304. Experiments show; the NHNK-632 has the effects of inhibiting proliferation and biofilm formation of pigment-generating pathogenic bacteria, agglutinating the pigment-generating pathogenic bacteria, inhibiting melanin generation of the pigment-generating pathogenic bacteria, inhibiting hyaluronidase activity of the pathogenic bacteria, promoting growth of gingival fibroblasts, inhibiting gingival cell inflammation and apoptosis induced by the pigment-generating pathogenic bacteria, and inhibiting melanin generation of the pigment-generating pathogenic bacteria. And the product can be used for preparing products for improving tooth color and gingivitis.

The invention discloses a traditional Chinese medicine mouthwash composition for strengthening teeth and refreshing oral cavity, the traditional Chinese medicine mouthwash composition comprises traditional Chinese medicine active components and a mouthwash matrix, and the traditional Chinese medicine active components comprise, by weight, 500-900 parts of ginseng, 10-20 parts of prunella vulgaris, 10-20 parts of coptis chinensis and 10-30 parts of turmeric. The invention also discloses a preparation method of the traditional Chinese medicine mouthwash composition. According to the traditional Chinese medicine mouthwash composition disclosed by the invention, the inhibition rate of anaerobic bacteria in the oral cavity can reach 42.1%-55.3%, the proliferation rate of gingival fibroblasts can reach up to 140.5%, the secretion amount of type I collagen of the gingival fibroblasts is increased to 24.4 ng / mL, and the effects of strengthening teeth and refreshing the oral cavity are remarkably realized.

The invention provides a coating composition. The coating composition comprises the following components: collagen, MXene, lipoic acid and a derivative thereof. All the components in the coating composition have a synergistic effect and jointly promote closing of soft tissue around an implant tooth, human gingival epithelial cells are promoted to better secrete laminin 332, more collagenous fibers secreted by human gingival fibroblasts are I-type collagen, and the coating composition is a polymer coating with an antibacterial function.

The invention belongs to the field of biomedical materials and bioengineering films, and particularly relates to a cross-magnetic dielectric effect material and a preparation method thereof, specifically, magnetic metal particles cobalt (Co) and dielectric ceramic (MgF2) are used as raw materials, and the cross-magnetic dielectric effect material is prepared on the surface of titanium through a multi-source sputtering deposition technology. The magnetic nano-particle multi-phase film with the TMD effect is prepared from the magnetic metal with high saturation magnetization and the dielectric ceramic with high dielectric constant. The titanium sheet with the TMD effect is co-cultured with mouse embryo osteoblast precursor cells (MC3T3-E1) and human gingival fibroblasts (HGF-1) to find that the TMD effect of the titanium sheet has good biological safety, and on the basis, preparation parameters of the multiphase membrane are optimized, so that the polarization of macrophages is regulated and controlled by proper magnetic field force generated by the TMD effect of the titanium sheet, and the macrophages with the TMD effect can be obtained. Therefore, osteogenic differentiation of bone marrow mesenchymal stem cells is promoted, titanium surface osseointegration is improved, and the method is expected to serve as a new direction for preparing a surface coating for promoting implant osseointegration.

Compositions of matter, including cell populations and medicaments, are provided that are derived from equine gingival fibroblasts and have cell phenotypes that occur in proportions not found in natural gum tissue, but rather, are preferentially are selected to express proteins favoring angiogenesis and anti-inflammatory effects, while reducing cell populations that promote tumorigenicity and / or formation of metalloproteinases that inhibit tissue regeneration. Methods of generating such compositions are provided that increase proliferation many-fold compared to previously known methods, and methods of using such compositions in a wide range of equine cell therapies are provided.

Compositions of matter, including cell populations and medicaments, are provided that are derived from equine gingival fibroblasts and have cell phenotypes that occur in proportions not found in natural gum tissue, but rather, are preferentially are selected to express proteins favoring angiogenesis and anti-inflammatory effects, while reducing cell populations that promote tumorigenicity and / or formation of metalloproteinases that inhibit tissue regeneration. Methods of generating such compositions are provided that increase proliferation many-fold compared to previously known methods, and methods of using such compositions in a wide range of equine cell therapies are provided.

Promoters of gingival fibroblast periostin production, compositions comprising the same, and methods of using the same are herein. Promoters of dental osteoblast cell mineralization, compositions comprising the same, and methods of using the same are also herein.

Compositions of matter, including cell populations and medicaments, are provided that are derived from human gingival fibroblasts and have cell phenotypes that occur in proportions not found in natural gum tissue, but rather, are preferentially are selected to express proteins favoring angiogenesis and anti-inflammatory effects, while reducing cell populations that promote tumorigenicity and / or formation of metalloproteinases that inhibit tissue regeneration. Methods of generating such compositions are provided that increase proliferation many-fold compared to previously known methods, and methods of using such compositions in a wide range of human cell therapies are provided.

The invention discloses an antibacterial peptide, a preparation method and application of the antibacterial peptide in preventing and treating oral problems, and belongs to the technical field of antibacterial peptides. The amino acid sequence of the antibacterial peptide is FIRLLKSAGKAIHKLIRRGH, and the amino acid sequence of the antibacterial peptide is shown in the description. In-vitro experiments show that the compound has broad-spectrum antibacterial activity on oral pathogens such as fusobacterium nucleatum, porphyromonas gingivalis and streptococcus mutans, the minimum inhibitory concentration is 31.25-125 mu g / mL, no obvious hemolysis effect exists, the therapeutic index reaches 50.79, the cell selectivity is high, and certain protection and anti-inflammatory effects are achieved in a Pg-induced human gingival fibroblast in-vitro inflammatory model. The traditional Chinese medicine composition has a certain potential effect of inhibiting oral gingivitis. The invention provides an important research and development thought for the development of novel oral disease treatment medicines and nursing products, and has a good application prospect.

This invention relates to an sPL nanomaterial for repairing gingival recession and its preparation method, specifically to an sPL nanomaterial for repairing gingival recession and its preparation method. The nanomaterial is composed of platelet lysis buffer, exosomes, graphene oxide, and CREKA peptide, wherein the exosomes are extracted from dental pulp stem cells. The method includes: Step 1: Extraction of exosomes; Step 2: Preparation of sPL; Step 3: Preparation of the Go-sPL-CREKA nanomaterial. The nanomaterial of this invention can promote the proliferation of gingival fibroblasts, increase the content of growth factors related to gingival recession repair, and enhance the repair effect of gingival recession. This invention is used for repairing gingival recession.

The present application relates to a kind of inhibiting gingival senile bleeding, repair gingival and maintain oral flora balance composition, belong to oral care technical field.The inhibiting gingival senile bleeding, repair gingival and maintain oral flora balance composition of the present application includes the following components: ECMP, white and extract, non-sertone.The inhibiting gingival senile bleeding, repair gingival and maintain oral flora balance composition of the present application has significantly improved clotting time, promotes the repair of gingival fibroblast, inhibits the expression of senile gene p53 and p21, and has certain proliferation effect on oral probiotics.

Compositions of matter, including cell populations and medicaments, are provided that are derived from canine gingival fibroblasts and have cell phenotypes that occur in proportions not found in natural gum tissue, but rather, are preferentially are selected to express proteins favoring angiogenesis and anti-inflammatory effects, while reducing cell populations that promote tumorigenicity and / or formation of metalloproteinases that inhibit tissue regeneration. Methods of generating such compositions are provided that increase proliferation many-fold compared to previously known methods, and methods of using such compositions in a wide range of canine cell therapies are provided.

The invention discloses a nano preparation as well as a preparation method and application thereof. The invention specifically provides a preparation method of a nano preparation and the nano preparation prepared by the method, the preparation method comprises the following steps: step 1, pretreating a pharmaceutical composition, mixing the pharmaceutical composition with a buffer solution, and carrying out ultrasonic centrifugation to obtain a water phase and a lower precipitate; and step 2, preparing the nano preparation from the organic phase and the water phase in the step 1 through a microfluidic method, the flow velocity of the organic phase is 1-5 mL / min, and the flow velocity of the water phase is 6-12 mL / min; the organic phase comprises the lower layer precipitate in the step 1, an alcohol solvent, phospholipid and sterol. The nano preparation disclosed by the invention can be used for treating oral diseases, and can effectively promote the growth of gingival fibroblasts and inhibit the content expression of IL-1alpha and TNF-alpha.

To provide type I collagen production promoters with high safety in normal human gingival fibroblasts having excellent type I collagen production promoting action in normal human gingival fibroblasts, and to provide oral agents with high safety having excellent type I collagen production promoting action in normal human gingival fibroblasts.SOLUTION: The present invention provides a type I collagen production promoter in normal human gingival fibroblasts containing at least one selected from the group consisting of dipotassium glycyrrhizinate, Lycium chinense Miller extract, Scutellaria baicalensis extract, Glycyrrhiza uralensis Fisch. extract, Lonicera japonica extract, Royal jelly extract, Guttiferae extract, Mulberry bark extract, and Rubus suavissimus S. Lee extract, and an oral agent containing the type I collagen production promoter in normal human gingival fibroblasts.SELECTED DRAWING: None