10 results about "Gingival fibroblast" patented technology

A composition is provided comprising a cell population and pharmaceuticals derived from human gingival fibroblasts, present in proportions not found in natural gingival tissue, but having a cellular phenotype preferentially selected to express proteins favorable for angiogenesis and anti-inflammatory effects, while reducing the formation of cell populations that promote tumorigenesis and / or metalloproteinases that inhibit tissue regeneration. A method for producing such a composition that increases proliferation several times over compared to previously known methods is provided, and a method for using such a composition in a wide range of human cell therapies is also provided.

This invention relates to the field of oral care products technology, specifically to an artificial enzyme with antibacterial properties, its preparation method, and its application. This technical solution employs a chemical reduction method, where a palladium source reacts in an environment containing surfactants and reducing agents to obtain palladium nanozymes. The obtained palladium nanozymes exhibit uniform morphology, controllable particle size, good dispersibility in water, and good biocompatibility; they have no significant toxic side effects on human gingival fibroblasts, possess highly efficient catalase activity, and demonstrate strong antibacterial and bactericidal effects against oral pathogens, significantly reducing bacterial drug resistance; they can inhibit acid production by oral bacteria, preventing tooth decay; and they retain their original catalytic activity even after multiple cycles of use. Applying the palladium nanozymes of this solution to the manufacture of oral care products can solve the technical problems of unsatisfactory safety and easy development of drug resistance in existing oral care products using antibacterial and anti-caries methods, showing promising application prospects.

The traditional Chinese medicine mouthwash comprises a traditional Chinese medicine compound and a mouthwash matrix, and the traditional Chinese medicine compound comprises the following components in parts by weight: 10-20 parts of golden cypress, 15-30 parts of angelica sinensis, 15-30 parts of honeysuckle, 15-30 parts of chrysanthemum and 15-30 parts of liquorice. The invention also provides a preparation method of the traditional Chinese medicine mouthwash for relieving gingivitis. The traditional Chinese medicine mouthwash for relieving gingivitis can promote proliferation of gingival fibroblasts, promote secretion of gingival fibroblast collagen and effectively inhibit proliferation of oral anaerobic bacteria, thereby relieving gingivitis.

The invention discloses an exosome and drug loaded gradient aperture periodontal regeneration composite membrane and a preparation method thereof, and belongs to the field of biomedical materials. According to the composite membrane, RGD peptide modified freeze-dried chitosan is adopted as an inner-layer macroporous membrane, silk fibroin loaded with periodontal ligament stem cell source exosome is adopted as a middle-layer mesoporous membrane, silk fibroin loaded with medicine is adopted as an outer-layer microporous membrane, and a three-layer gradient aperture structure is constructed through an electrostatic spinning and freeze-drying combined technology. The interlayer bonding strength is improved through interface spraying of polyvinyl alcohol, the biological activity is guaranteed through an exosome freeze-drying protection technology, and time sequence synergistic release of rapid anti-inflammation of drugs and long-acting regeneration promotion of exosomes is achieved. The composite membrane has excellent mechanical property, degradation property and biological activity, can remarkably promote gingival fibroblast COL-I secretion and alveolar bone regeneration, and is applied to periodontitis tissue regeneration and repair.

This invention belongs to the field of guided bone regeneration materials technology, specifically relating to an arginine carbon dot photoinitiated hydrogel prepolymer, its preparation method, and its application. The arginine carbon dot photoinitiated hydrogel prepolymer of this invention, when used for alveolar bone regeneration, inhibits the proliferation / migration of gingival fibroblasts (hGFs) while simultaneously inducing and promoting the proliferation / migration and osteogenic differentiation of bone marrow mesenchymal stem cells (hBMSCs), thus creating a "reverse-response" cell-selective regulatory effect.

The application discloses a Streptococcus salivarius CCFM1512 which can inhibit excessive inflammatory response and relieve periodontitis, and belongs to the technical field of microorganisms. The fermentation supernatant of the Streptococcus salivarius provided by the application can relieve inflammation of human gingival fibroblasts induced by Porphyromonas gingivalis lipopolysaccharide in vitro, and can significantly reduce the expression of inflammatory factors such as IL-1beta, IL-6 and IL-17A in gingival tissues, regulate the M1 / M2 polarization balance of macrophages and restore the expression level of DEL-1, so as to relieve alveolar bone absorption of rats. The Streptococcus salivarius has a great application prospect in the aspects of regulating oral inflammatory response and preparing products for relieving periodontitis.

The invention discloses a traditional Chinese medicine mouthwash composition for strengthening teeth and refreshing oral cavity, the traditional Chinese medicine mouthwash composition comprises traditional Chinese medicine active components and a mouthwash matrix, and the traditional Chinese medicine active components comprise, by weight, 500-900 parts of ginseng, 10-20 parts of prunella vulgaris, 10-20 parts of coptis chinensis and 10-30 parts of turmeric. The invention also discloses a preparation method of the traditional Chinese medicine mouthwash composition. According to the traditional Chinese medicine mouthwash composition disclosed by the invention, the inhibition rate of anaerobic bacteria in the oral cavity can reach 42.1%-55.3%, the proliferation rate of gingival fibroblasts can reach up to 140.5%, the secretion amount of type I collagen of the gingival fibroblasts is increased to 24.4 ng / mL, and the effects of strengthening teeth and refreshing the oral cavity are remarkably realized.

Promoters of gingival fibroblast periostin production, compositions comprising the same, and methods of using the same are herein. Promoters of dental osteoblast cell mineralization, compositions comprising the same, and methods of using the same are also herein.

Compositions of matter, including cell populations and medicaments, are provided that are derived from human gingival fibroblasts and have cell phenotypes that occur in proportions not found in natural gum tissue, but rather, are preferentially are selected to express proteins favoring angiogenesis and anti-inflammatory effects, while reducing cell populations that promote tumorigenicity and / or formation of metalloproteinases that inhibit tissue regeneration. Methods of generating such compositions are provided that increase proliferation many-fold compared to previously known methods, and methods of using such compositions in a wide range of human cell therapies are provided.

The invention discloses an antibacterial peptide, a preparation method and application of the antibacterial peptide in preventing and treating oral problems, and belongs to the technical field of antibacterial peptides. The amino acid sequence of the antibacterial peptide is FIRLLKSAGKAIHKLIRRGH, and the amino acid sequence of the antibacterial peptide is shown in the description. In-vitro experiments show that the compound has broad-spectrum antibacterial activity on oral pathogens such as fusobacterium nucleatum, porphyromonas gingivalis and streptococcus mutans, the minimum inhibitory concentration is 31.25-125 mu g / mL, no obvious hemolysis effect exists, the therapeutic index reaches 50.79, the cell selectivity is high, and certain protection and anti-inflammatory effects are achieved in a Pg-induced human gingival fibroblast in-vitro inflammatory model. The traditional Chinese medicine composition has a certain potential effect of inhibiting oral gingivitis. The invention provides an important research and development thought for the development of novel oral disease treatment medicines and nursing products, and has a good application prospect.