20 results about "Artificial enzyme" patented technology

This invention relates to the field of oral care products technology, specifically to an artificial enzyme with antibacterial properties, its preparation method, and its application. This technical solution employs a chemical reduction method, where a palladium source reacts in an environment containing surfactants and reducing agents to obtain palladium nanozymes. The obtained palladium nanozymes exhibit uniform morphology, controllable particle size, good dispersibility in water, and good biocompatibility; they have no significant toxic side effects on human gingival fibroblasts, possess highly efficient catalase activity, and demonstrate strong antibacterial and bactericidal effects against oral pathogens, significantly reducing bacterial drug resistance; they can inhibit acid production by oral bacteria, preventing tooth decay; and they retain their original catalytic activity even after multiple cycles of use. Applying the palladium nanozymes of this solution to the manufacture of oral care products can solve the technical problems of unsatisfactory safety and easy development of drug resistance in existing oral care products using antibacterial and anti-caries methods, showing promising application prospects.

This invention discloses a cerium oxide nanozyme derivative and its preparation method. The method first prepares a cerium oxide nanorod precursor via precipitation, then further prepares a cerium oxide nanozyme using a hydrothermal method, and finally encapsulates the polymer and the cerium oxide nanozyme in a specific ratio to obtain the cerium oxide nanozyme derivative. The novel cerium oxide nanozyme derivative prepared by this invention has a lower surface charge, enhanced adsorption of chromogenic substrates, improved catalytic performance compared to single cerium oxide enzymes, and expands the types of nanozymes, providing possibilities for further applications in environmental remediation and protection. This invention develops a cerium oxide nanozyme derivative material and provides a simple and feasible method for preparing artificial enzyme nanomaterials.

Provided herein are artificial enzymes comprising a solid substrate core coated in a shell that is electrochemically inert but conductive. Also provided are electrodes comprising said artificial enzymes and a support structure. Also provided are methods of selectively detecting at least one analyte and / or obtaining the concentration of the analyte in a sample using the electrode. Also provided are methods of preparing the artificial enzymes and the electrode and artificial enzymes and electrodes produced using those methods.

The invention discloses an iron-oxygen-based artificial enzyme as well as a preparation method and application thereof. The method comprises the following steps: preparing a chelating layer on a basement membrane; mixing ferric chloride hexahydrate, ferrous sulfate heptahydrate, histidine and a solvent to obtain a mineralized precursor solution, and removing oxygen in the mineralized precursor solution; the mineralization precursor solution is spread on the chelating layer to form a solution layer, the mineralization precursor solution of the solution layer is subjected to a mineralization reaction in an alkaline environment, and a mineral layer is generated on the chelating layer. According to the method, the histidine is added into the mineralization precursor solution, the mineralization process of the mineralization precursor solution is intervened, more ferrihydrite and delta-iron oxyhydroxide stably exist in a mineral layer, meanwhile, the histidine on the surfaces of various mineral crystals also creates a chiral microenvironment, and a chiral microenvironment is formed. And the mineral layer realizes the synchronous improvement of the activities of multiple enzymes such as CAT-like, POD-like and SOD-like, and is beneficial to the multifunctionality of the iron-based artificial enzyme in different scenes.

The invention provides a double transition metal doped platinum sulfide-cyclodextrin artificial enzyme and a preparation method and application thereof, the preparation method comprises the following steps: (1) dispersing cyclodextrin in an ethanol solution containing Pt < 2 + > and transition metal ions, and stirring in a protective atmosphere to obtain a suspension; the transition metal ions are selected from at least two of Mn < 2 + >, Fe < 2 + >, Co < 2 + >, Ni < 2 + >, Cu < 2 + > and Zn < 2 + >; (2) carrying out solid-liquid separation on the suspension, wherein the obtained solid is cyclodextrin adsorbed with Pt < 2 + > and transition metal ions; (3) dispersing cyclodextrin adsorbed with Pt < 2 + > and transition metal ions, thiourea and hexadecyl trimethyl ammonium bromide into absolute ethyl alcohol, and performing solvothermal reaction to obtain a reaction solution; and (4) carrying out solid-liquid separation on the reaction liquid, wherein the obtained solid is the double transition metal doped platinum sulfide-cyclodextrin artificial enzyme. The double transition metal doped platinum sulfide-cyclodextrin artificial enzyme catalyst is ingenious in design, novel in structure, stable in property, low in cost and wide in application range.

Disclosed is a method of producing a non-naturally occurring peptide for reducing nitrogen, the method comprising: a) identifying a first group of peptides each having a binding pocket similar to a naturally occurring iron-sulfur cluster cofactor binding pocket of a naturally occurring nitrogenase, wherein each of the first group of peptides is not a nitrogenase or a subunit or domain of a nitrogenase; b) selecting, from the first group of peptides, a second group of peptides each having at least one target amino acid in the binding pocket; c) selecting, from the second group of peptides, a third group of peptides each having at least one target property; and d) modifying the sequence and / or structure of each of the third group of peptides to improve binding of the iron-sulfur cluster cofactor in the binding pocket. Also disclosed is a non-naturally occurring peptide for reducing nitrogen, comprising: at least one modification to a naturally occurring peptide; and a binding pocket for binding an iron-sulfur cluster cofactor; wherein the naturally occurring peptide is not a nitrogenase or a subunit or domain of a nitrogenase; and wherein the at least one modification improves binding of the iron-sulfur cluster cofactor in the binding pocket.

The invention provides a binary rare earth metal alloy particle (at) porphyrin COF mimic enzyme, which is prepared by the following steps: suspending porphyrin COF in an ethanol solution containing two trivalent rare earth metal ions, stirring for 1.0-12.0 hours at 0-60 DEG C in an inert gas atmosphere, sequentially carrying out suction filtration and ethanol washing on the obtained reaction mixture to obtain a porphyrin COF solid adsorbing binary rare earth metal ions, and drying to obtain the binary rare earth metal alloy particle (at) porphyrin COF mimic enzyme. The preparation method comprises the following steps: taking porphyrin COF as a substrate, suspending the porphyrin COF in absolute ethyl alcohol, stirring and dropwise adding an ethanol solution of NaBH4 at 0-60 DEG C in an inert gas atmosphere, stirring and reacting for 3.0-24.0 hours in a dark place, and performing post-treatment on the obtained suspension to obtain binary rare earth metal alloy particles, namely the porphyrin COF mimic enzyme; the catalyst can catalyze visible light to drive CO2 to be reduced to prepare ethylene and react at room temperature, visible light is used as an energy source, water is used as a solvent and a hydrogen source, and the catalyst is green, environment-friendly, energy-saving, emission-reducing, high in reduction product generation efficiency, high in ethylene selectivity, high in practicability, green, efficient, practical and large in industrial application potential.

The invention belongs to the technical field of biological catalytic materials, and particularly relates to a gold monatomic radiosensitizer with multienzyme activity as well as a preparation method and application of the gold monatomic radiosensitizer. The gold monatomic radiosensitizer is a coordination complex formed by a gold source containing tetrachloro gold (III) acid radical ions and 2, 4, 6-tri (4-pyridyl)-1, 3, 5-triazine through a coordination substitution reaction of Au and pyridine nitrogen. The gold monatomic radiosensitizer disclosed by the invention is a biocatalyst which is newly designed, has multi-enzyme activity biological catalysis performance and can be activated by radiation, and can be used as a polymer artificial enzyme for efficient and controllable tumor treatment.

The invention discloses a design and construction method and application of a novel Friedel-Crafts alkylating enzyme based on non-natural cysteine, and belongs to the technical field of synthetic biology. According to the present invention, the 15th valine of the enzyme LmrR is replaced with p-aminobenzene cysteine to construct the artificial enzyme LmrRpAPhC, the mutant with improved chiral selectivity is further obtained through site-specific mutagenesis, and the ee value of the mutant LmrRV15pAPhCF93L achieves 82 + / -0%, and is improved by 20% compared with the ee value before mutation. Good application prospects are shown.

The application relates to the technical field of detection methods, in particular to a quantitative detection kit and detection method for the total amount of phytosterols in rapeseed. In the detection of the total amount of phytosterols in rapeseed, the peroxidase mimics of copper-based MOF carbide in the application are used as artificial catalytic enzymes to replace the conventional biological horseradish peroxidase, so that the cost is reduced, the stability of the method is improved, the signal stability of biological enzyme catalysis is inferior to that of artificial enzyme, and the accuracy of quantitative effect is also inferior to that of artificial enzyme.

The invention discloses a xanthine oxidase (XOD) analysis sensing method based on a tetrahedral programmable artificial enzyme and application of the xanthine oxidase (XOD) analysis sensing method in liver cancer CTCs. According to the sensor, a Poly-A tetrahedral gold cluster is used as a catalyst, H2O2 decomposition is effectively promoted, and good electrochemical response is achieved. H2O2 is generated through an enzyme reaction channel constructed by xanthine (XO) and XOD, and electrochemical signal enhancement can be observed, so that XOD and XO detection is indirectly realized. Cell experiment results show that compared with LO2 normal cells, XOD activity in HepG2 cancer cells is lower. Under the stimulation of H2O2, the XOD activity of the two cells is up-regulated, but the up-regulation amplitude of the HepG2 cell is small; under the action of exogenous xanthine, the XOD activity of LO2 and HepG2 cells is obviously increased, and the XOD activity in the HepG2 cells is more obviously improved. Comprehensively, the sensor can be used for efficiently detecting the activity of the XOD in the liver cancer CTCs, and the huge application potential of the sensor in biomedical research and clinical diagnosis is shown.

The invention discloses a choline dehydrogenase analysis sensing method based on a tetrahedral programmable artificial enzyme and application of the choline dehydrogenase analysis sensing method in gastric cancer. According to the sensor, a Poly-A tetrahedral gold cluster is used as a catalyst, hydrogen peroxide (H2O2) is effectively promoted to be decomposed to generate hydroxyl free radicals (. OH), the free radicals oxidize o-phenylenediamine (OPD) from a colorless state to yellow oxOPD, and a fluorescence emission peak at the wavelength of 563 nm and an ultraviolet absorption peak at the wavelength of 420 nm are remarkably enhanced. Furthermore, H2O2 is generated through an enzyme reaction channel constructed by choline (Ch) and choline dehydrogenase (CHDH), and enhancement of signal peaks can also be observed at the two wavelengths, so that analysis and detection of CHDH activity are indirectly realized, and further effective screening of the CHDH inhibitor is realized. The intelligent sensor has the advantages of high accuracy, quick response, high sensitivity, excellent selectivity, low cost and the like, and shows huge application potential in biomedical research and clinical diagnosis.

The invention belongs to the technical field of artificial mimic enzymes, and discloses a carboxylesterase simulant with phthalate degradation activity as well as a preparation method and application of the carboxylesterase simulant. The stimulant is Zn (II) (at) IMD PDA: beta-CD, and the structure of the stimulant takes beta-cyclodextrin as a skeleton, is bridged with an imidazole group through 1, 3-propane diamine, and is coordinated with Zn (II) ions to form a catalytic center. According to the design, a hydrophobic cavity of beta-cyclodextrin and a Zn (II)-imidazole active center are integrated into a whole, so that efficient recognition and catalytic hydrolysis of phthalate pollutants are realized. According to the preparation method, beta-cyclodextrin is taken as an initial raw material, one-pot three-step continuous reaction synthesis is carried out, the process is simple and convenient, the condition is mild, the cost is low, and the use of precious metals and tedious separation and purification steps are avoided. The simulant shows high-efficiency degradation capacity to various phthalic acid esters (such as DEHP, DBP and the like) under mild conditions, the degradation rate to the DEHP within 72 hours can reach 81.4%, and the activity of the simulant is still kept to be 98% or above after the simulant is recycled for 5 times.

The present application discloses an artificial enzyme, which is prepared based on sequence modification of a natural enzyme having the activity of catalyzing the reaction of converting compound I (nicotine) into compound II (N-methyl-4-hydroxy-3-methylnicotinamide) shown as follows, and compound II (N-methyl-4-hydroxy-3-methylnicotinamide) can spontaneously hydrate to generate compound III (pseudo-oxidized nicotine), or the natural enzyme is nicotine dehydrogenase; the modification includes replacing at least one of the amino acids that hinder the release of the product with an amino acid with a smaller side chain. The artificial enzyme has a higher catalytic rate on the substrate than the natural enzyme, and is very promising in the preparation of drugs for treating nicotine addiction.

This application discloses the application of Fe / Mn bimetallic enzymes in the preparation of anti-periodontalgia or teeth whitening agents. The Fe / Mn bimetallic enzyme exhibits excellent photothermal properties and peroxidase-like activity; that is, under photothermal synergy, its POD-like activity can be significantly enhanced, generating more ·OH groups. In antibacterial coating experiments against *Porphyromonas gingivalis* and drug-resistant *Staphylococcus aureus*, Fe / Mn BA, through photothermal synergistic chemokinetic therapy, achieved antibacterial rates exceeding 90%. By treating periodontitis through anti-inflammatory and antibacterial action and achieving teeth whitening through pigment degradation, the application of this biatomic nanozyme provides a new research direction for the application of artificial enzymes in oral diseases and is expected to open up broader pathways to address the challenges currently faced by artificial enzyme antibacterial technologies.

Disclosed is a method of producing a non-naturally occurring peptide for reducing nitrogen, the method comprising: a) identifying a first group of peptides each having a binding pocket similar to a naturally occurring iron-sulfur cluster cofactor binding pocket of a naturally occurring nitrogenase, wherein each of the first group of peptides is not a nitrogenase or a subunit or domain of a nitrogenase; b) selecting, from the first group of peptides, a second group of peptides each having at least one target amino acid in the binding pocket; c) selecting, from the second group of peptides, a third group of peptides each having at least one target property; and d) modifying the sequence and / or structure of each of the third group of peptides to improve binding of the iron-sulfur cluster cofactor in the binding pocket.

The application belongs to the technical field of biological catalytic materials, and particularly relates to a gold monatomic radiosensitizer with multiple enzyme activities and a preparation method and application thereof. The gold monatomic radiosensitizer is a coordination complex formed by a coordination substitution reaction of a gold source containing a tetrachlorogold (III) acid radical ion and 2,4,6-tris (4-pyridyl) -1,3,5-triazine through Au and pyridine nitrogen. The gold monatomic radiosensitizer disclosed in the application is a newly designed biological catalyst with multiple enzyme activities and radioactivation, and can be used as a polymer artificial enzyme for efficient and controllable tumor treatment.

The invention discloses a nano-enzyme filtering membrane as well as a preparation method and application thereof. According to the preparation method disclosed by the invention, uniform dispersion on polyamide acid (PAA) is realized by utilizing the amphipathy of cobalt salicylic acid N-octyl imidazole ionic liquid, and the activity of the obtained polyamide acid cobalt (PAA-Co) composite membrane is endowed by reduction activation. The PAA-Co composite membrane has excellent catalytic performance and stability, so that the PAA-Co composite membrane becomes an ideal candidate for dimethoate colorimetric detection. In addition, the PAA-Co membrane is integrated into the filtering device, and catalytic oxidation occurs in the process of filtering the soil test solution. When the solution passes through the membrane, impurities are effectively filtered out, so that the detection precision is improved, and meanwhile, a catalytic oxidation reaction occurs. According to the method, continuous testing is achieved, the trouble of artificial enzyme recovery is eliminated, the detection efficiency is remarkably improved, and it is proved that the method has huge potential in actual pesticide residue detection application.

The invention discloses a bacterium-iron monatomic hybrid based on an artificial enzyme nanomaterial as well as a preparation method and application of the bacterium-iron monatomic hybrid. The preparation method comprises the following steps: firstly, preparing an artificial enzyme nano material ISA (an iron-nitrogen coordination structure material taking a carbon substrate as a skeleton), and anchoring the artificial enzyme nano material ISA on the surface of a Cupriavidus necator membrane to form a bacterium-iron monoatomic hybrid (C.N at ISA); when the C.N (at) ISA is inoculated into a microbial electrosynthesis reactor taking hydrogen as an intermediate medium, an iron-nitrogen structure on the surface of the membrane can efficiently capture hydrogen generated by electrolyzed water and promote the utilization of hydrogen by bacteria, and meanwhile, the stress resistance of the bacteria to the environment is improved. At the moment, high-content reducing power (NADH) and energy carrier (ATP) are generated in the thalli of the C.N-ISA, the carbon sequestration behavior of the bacteria is strongly driven, and finally efficient production of the poly-beta-hydroxybutyrate with carbon dioxide as the carbon source is achieved.

The application discloses a design construction method of an artificial enzyme containing a non-natural tertiary amine catalytic center and application thereof, and is characterized in that the application introduces a tertiary amine cofactor with catalytic activity into the 89th position of LmrR by covalent linkage, and successfully constructs a new artificial enzyme LmrR_M89C_TA after protein purification. The artificial enzyme LmrR_M89C_TA containing the tertiary amine catalytic center constructed by the application can catalyze a non-natural asymmetric [3+2] cycloaddition reaction, and a chiral spirocyclic product is obtained at a conversion rate of 10% and an enantioselectivity of 84%.