10 results about "Artificial enzyme" patented technology

This invention relates to the field of oral care products technology, specifically to an artificial enzyme with antibacterial properties, its preparation method, and its application. This technical solution employs a chemical reduction method, where a palladium source reacts in an environment containing surfactants and reducing agents to obtain palladium nanozymes. The obtained palladium nanozymes exhibit uniform morphology, controllable particle size, good dispersibility in water, and good biocompatibility; they have no significant toxic side effects on human gingival fibroblasts, possess highly efficient catalase activity, and demonstrate strong antibacterial and bactericidal effects against oral pathogens, significantly reducing bacterial drug resistance; they can inhibit acid production by oral bacteria, preventing tooth decay; and they retain their original catalytic activity even after multiple cycles of use. Applying the palladium nanozymes of this solution to the manufacture of oral care products can solve the technical problems of unsatisfactory safety and easy development of drug resistance in existing oral care products using antibacterial and anti-caries methods, showing promising application prospects.

The invention provides a double transition metal doped platinum sulfide-cyclodextrin artificial enzyme and a preparation method and application thereof, the preparation method comprises the following steps: (1) dispersing cyclodextrin in an ethanol solution containing Pt < 2 + > and transition metal ions, and stirring in a protective atmosphere to obtain a suspension; the transition metal ions are selected from at least two of Mn < 2 + >, Fe < 2 + >, Co < 2 + >, Ni < 2 + >, Cu < 2 + > and Zn < 2 + >; (2) carrying out solid-liquid separation on the suspension, wherein the obtained solid is cyclodextrin adsorbed with Pt < 2 + > and transition metal ions; (3) dispersing cyclodextrin adsorbed with Pt < 2 + > and transition metal ions, thiourea and hexadecyl trimethyl ammonium bromide into absolute ethyl alcohol, and performing solvothermal reaction to obtain a reaction solution; and (4) carrying out solid-liquid separation on the reaction liquid, wherein the obtained solid is the double transition metal doped platinum sulfide-cyclodextrin artificial enzyme. The double transition metal doped platinum sulfide-cyclodextrin artificial enzyme catalyst is ingenious in design, novel in structure, stable in property, low in cost and wide in application range.

The invention provides a binary rare earth metal alloy particle (at) porphyrin COF mimic enzyme, which is prepared by the following steps: suspending porphyrin COF in an ethanol solution containing two trivalent rare earth metal ions, stirring for 1.0-12.0 hours at 0-60 DEG C in an inert gas atmosphere, sequentially carrying out suction filtration and ethanol washing on the obtained reaction mixture to obtain a porphyrin COF solid adsorbing binary rare earth metal ions, and drying to obtain the binary rare earth metal alloy particle (at) porphyrin COF mimic enzyme. The preparation method comprises the following steps: taking porphyrin COF as a substrate, suspending the porphyrin COF in absolute ethyl alcohol, stirring and dropwise adding an ethanol solution of NaBH4 at 0-60 DEG C in an inert gas atmosphere, stirring and reacting for 3.0-24.0 hours in a dark place, and performing post-treatment on the obtained suspension to obtain binary rare earth metal alloy particles, namely the porphyrin COF mimic enzyme; the catalyst can catalyze visible light to drive CO2 to be reduced to prepare ethylene and react at room temperature, visible light is used as an energy source, water is used as a solvent and a hydrogen source, and the catalyst is green, environment-friendly, energy-saving, emission-reducing, high in reduction product generation efficiency, high in ethylene selectivity, high in practicability, green, efficient, practical and large in industrial application potential.

The invention discloses a xanthine oxidase (XOD) analysis sensing method based on a tetrahedral programmable artificial enzyme and application of the xanthine oxidase (XOD) analysis sensing method in liver cancer CTCs. According to the sensor, a Poly-A tetrahedral gold cluster is used as a catalyst, H2O2 decomposition is effectively promoted, and good electrochemical response is achieved. H2O2 is generated through an enzyme reaction channel constructed by xanthine (XO) and XOD, and electrochemical signal enhancement can be observed, so that XOD and XO detection is indirectly realized. Cell experiment results show that compared with LO2 normal cells, XOD activity in HepG2 cancer cells is lower. Under the stimulation of H2O2, the XOD activity of the two cells is up-regulated, but the up-regulation amplitude of the HepG2 cell is small; under the action of exogenous xanthine, the XOD activity of LO2 and HepG2 cells is obviously increased, and the XOD activity in the HepG2 cells is more obviously improved. Comprehensively, the sensor can be used for efficiently detecting the activity of the XOD in the liver cancer CTCs, and the huge application potential of the sensor in biomedical research and clinical diagnosis is shown.

The present application discloses an artificial enzyme, which is prepared based on sequence modification of a natural enzyme having the activity of catalyzing the reaction of converting compound I (nicotine) into compound II (N-methyl-4-hydroxy-3-methylnicotinamide) shown as follows, and compound II (N-methyl-4-hydroxy-3-methylnicotinamide) can spontaneously hydrate to generate compound III (pseudo-oxidized nicotine), or the natural enzyme is nicotine dehydrogenase; the modification includes replacing at least one of the amino acids that hinder the release of the product with an amino acid with a smaller side chain. The artificial enzyme has a higher catalytic rate on the substrate than the natural enzyme, and is very promising in the preparation of drugs for treating nicotine addiction.

This application discloses the application of Fe / Mn bimetallic enzymes in the preparation of anti-periodontalgia or teeth whitening agents. The Fe / Mn bimetallic enzyme exhibits excellent photothermal properties and peroxidase-like activity; that is, under photothermal synergy, its POD-like activity can be significantly enhanced, generating more ·OH groups. In antibacterial coating experiments against *Porphyromonas gingivalis* and drug-resistant *Staphylococcus aureus*, Fe / Mn BA, through photothermal synergistic chemokinetic therapy, achieved antibacterial rates exceeding 90%. By treating periodontitis through anti-inflammatory and antibacterial action and achieving teeth whitening through pigment degradation, the application of this biatomic nanozyme provides a new research direction for the application of artificial enzymes in oral diseases and is expected to open up broader pathways to address the challenges currently faced by artificial enzyme antibacterial technologies.

Disclosed is a method of producing a non-naturally occurring peptide for reducing nitrogen, the method comprising: a) identifying a first group of peptides each having a binding pocket similar to a naturally occurring iron-sulfur cluster cofactor binding pocket of a naturally occurring nitrogenase, wherein each of the first group of peptides is not a nitrogenase or a subunit or domain of a nitrogenase; b) selecting, from the first group of peptides, a second group of peptides each having at least one target amino acid in the binding pocket; c) selecting, from the second group of peptides, a third group of peptides each having at least one target property; and d) modifying the sequence and / or structure of each of the third group of peptides to improve binding of the iron-sulfur cluster cofactor in the binding pocket.

The application belongs to the technical field of biological catalytic materials, and particularly relates to a gold monatomic radiosensitizer with multiple enzyme activities and a preparation method and application thereof. The gold monatomic radiosensitizer is a coordination complex formed by a coordination substitution reaction of a gold source containing a tetrachlorogold (III) acid radical ion and 2,4,6-tris (4-pyridyl) -1,3,5-triazine through Au and pyridine nitrogen. The gold monatomic radiosensitizer disclosed in the application is a newly designed biological catalyst with multiple enzyme activities and radioactivation, and can be used as a polymer artificial enzyme for efficient and controllable tumor treatment.