45 results about "Artificial enzyme" patented technology

The present invention concerns a novel means by which specific chosen reactions can be accelerated through the use of a new type of artificial enzyme. The invention allows specific reactions to occur at an accelerated rate, even in the presence of other non-chosen molecules, which may be very similar in structure to the chosen reactant. The reactions may be stoichiometric or catalytic.

The invention relates to a process for preparing silk functional fabric, which grafts the cyclodextrin onto the silk fiber through the chemical bond to obtain the silk functional fabric product with cyclodextrin solid chirality hydrophobic cavity, wherein the process can be used for preparing fabric with aromatic and medical health care functions and also can be used in the fields of molecule identification, waste water purification, artificial enzyme system forming and pharmaceutical slow releasing.

The invention relates to a process for preparing sericin silk functional fiber, which comprises: processing the sericin fixation on the sericin silk, then grafting cyclodextrin onto the silk fiber through chemical bond to obtain the sericin silk functional fiber product with cyclodextrin solid chirality hydrophobic cavity. The process for preparartion can be applied in the preparation of aromatic and medical health care functional fiber and also can be applied in the fields of molecule identification, waste water purification, artificial enzyme system forming and pharmaceutical slow release.

The invention relates to the technical field of biology, particularly a DNA (deoxyribonucleic acid) three-dimensional nano-structure artificial enzyme precursor, and preparation and application thereof. The invention provides a stable chemical environment by using a DNA tetrahedron as a nuclease framework; and by utilizing the controllability of the DNA tetrahedron nano structure, nucleases are respectively connected to different positions of the DNA tetrahedron framework, thereby forming the DNA tetrahedron nano-structure artificial enzyme precursor with different structures. Compared with the traditional nucleases, the enzyme formed by the DNA tetrahedron nano-structure artificial enzyme precursor with different structures has more stable structure and higher sensitivity, and the catalytic activity is 6-14 times of the traditional nuclease.

A method for improving peroxidase-like activity of nanozyme and a product thereof are disclosed, which relate to the field of artificial enzymes in biochemistry. The method adopts a hydrogen peroxide solution with high concentration to treat the VO₂(B) powder for obtaining a product with high peroxidase-like activity. Compared with the pure VO₂(B) powder, the peroxidase-like activity of the product obtained by the method is increased by 4 to 12 times. The method provided by the present invention adopts raw materials with low cost and mild reaction conditions, is simple in operation and low in cost, which is conducive to batch preparation. The powder product obtained by the method is able to be applied to detect hydrogen peroxide, glucose, etc., and has great application prospects in biosensing, industrial wastewater treatment and sewage treatment.

This invention is to provide a catalyst (an artificial enzyme) which can be used as an alternative to a protein enzyme in the field relating to medicine, pharmaceuticals, biochemistry or chemical engineering. Such a catalyst comprises a complex of a transition metal and a monomeric or polymeric nucleotide or an analogue thereof.

Provides an artificial enzyme obtained by improving upon a sequence of a natural nicotine dehydrogenase, wherein the improvement comprises replacing at least one amino acid hindering product release with an amino acid with smaller side chains, thereby improving a catalytic rate.

A method of making nicotinamide mononucleotide (NMN), nicotinamide mononucleotide derivatives, or mixtures thereof is disclosed. The method involves the in vitro artificial enzymatic pathways comprised: the generation of alpha-D-ribose-1-phosphate from numerous substrates followed by the synthesis of nicotinamide mononucleotide catalyzed by nicotinamide riboside phosphorylase and nicotinamide riboside kinase or the generation of 5-phospho-alpha-D-ribose-1-diphosphate from nucleotides followed by the synthesis of nicotinamide mononucleotide catalyzed by nicotinamide phosphoribosyltransferase. The multiple enzymes were reconstituted in one pot, wherein in-situ removal of byproducts that can be converted to other non-inhibitory chemicals with supplementary enzymes push the overall biotransformation toward the synthesis of nicotinamide mononucleotide. Furthermore, nicotinamide mononucleotide can be converted to its derivatives—nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate.

The method for producing an artificial enzyme according to the invention is a method for producing an artificial enzyme and includes the step of selecting an artificial enzyme precursor which includes an oligonucleotide sequence containing modified nucleosides prepared by introducing a substituent into each nucleoside and at least one of the modified nucleoside capable of reacting with a raw substance of a target reaction which the artificial enzyme catalyzes; and the step of producing the artificial enzyme which is capable of catalyzing a target reaction and includes the oligonucleotide sequence in which the modified nucleoside capable of reacting with the raw substance of the artificial enzyme precursor is substituted with a non-reactive modified nucleoside which is non-reactive with the raw substance of the target reaction.

The invention discloses a glutathione peroxidase GPX2 mutant containing serine and a preparation method of the mutant, and belongs to the field of biotechnology. The novel high-activity glutathione peroxidase GPX2 mutant is obtained by taking human GPX2 as a template through computer simulation and site-specific mutagenesis, and the mutant consists of 203 amino acids; compared with the human GPX2, the mutant is free from cysteine, and is relatively high in GPX activity and better in stability; all cysteine in the human GPX2 is mutated into serine, but the 40th site is still selenocysteine (SeCys), alanine (Ala) on the 117th-site is mutated into serine (Ser), and a formed sequence is as shown in SEQ ID No:2. The method can achieve the direct expression of GPX2 mutant protein having high enzymatic activity in mammalian cell lines or auxotroph prokaryotic expression system and SPP low-temperature expression system thereof by virtue of genetic engineering technology. The method can prepare a novel artificial enzyme having quite high GPX activity without chemical modification. The method disclosed by the invention has a broad application prospect in the aspect of biological pharmacy.

The invention discloses an artificial photosynthesis system and an application thereof. The artificial photosynthesis system comprises an artificial enzyme carrier, a light capture agent and an artificial enzyme, wherein the artificial enzyme comprises a coordination compound formed by metal ions and more than two nitrogen-atoms in melamine molecule in the artificial enzyme carrier. The artificial photosynthesis system contains 0.5-6wt% of melamine. The artificial photosynthesis system provided by the invention takes the melamine as the photosensitizer and the artificial enzyme carrier and the coordination compound formed between metal ions and nitrogen-atoms as the artificial enzyme, so that the selectivity of the photocatalytic reduction products to methyl alcohol is increased. The artificial photosynthesis system provided by the invention puts forward taking the semiconductor material ceric oxide coupling metal complex as the artificial enzyme for constructing a theoretical model for the artificial photosynthesis system, so that the applications of the semiconductor material CeO2 and melamine in the photocatalysis field are widened, the photocatalyst preparation process according to the invention is simple and the raw materials are low-cost and easily acquired.

The invention relates to the technical field of food processing, and particularly relates to a making method for improving the quality of whole wheat bread. The making method comprises the following steps of performing enzymolysis on wheat bran by a natural and artificial complex enzyme method, performing wheat germination treatment through safe and effective sterilization of artificial enzymes, preparing malt flour, and adding the wheat bran after enzymolysis, the malt flour and konjac gum to high gluten flour to make bran-containing whole wheat bread. Through enzymolysis of complex enzymes,crude fibers in the wheat bran are degraded, the roughness of the bread is reduced, non-starch polysaccharide in whole wheat flour is hydrolyzed into water-soluble sugar, and the mouth feel is improved; and through the stickiness of the konjac gum, the whole wheat flour is promoted to form gluten in mixing, and the whole wheat bread is easy to process and shape, so that the limitation of the content of the whole wheat flour is broken through. In the baking process of the konjac gum, a reticular tissue structure having supporting effects is formed, and generated gas is wrapped in the reticularstructure, so that the texture of the bread is soft. Besides, sterilization treatment of the artificial enzymes is used, so that the shelf life of the bread is prolonged.

The invention relates to a 4D intelligent aerogel and a preparation method thereof. The preparation method is characterized by regulating the shape memory property and regulating the microstructure, the density and the hydrophilicity. The 4D intelligent aerogel is prepared by a one-step crosslinking vacuum freeze-drying process, and the technical problems of complexness and poor controllability ofexisting intelligent aerogel preparation methods are solved. The preparation method has the advantages of simple preparation process, low cost, good controllability, strong universality, and easinessin modification and compounding of a material. The 4D aerogel has the advantages of strong controllability, good structural designability, good density and hydrophilicity controllability and good mechanical properties, can be used in fields of marine development, intelligent drugs, environmental protection, porous electrodes, intelligent adsorption, intelligent artificial enzymes, intelligent sound absorption, new energy development, intelligent protection, building materials, lightweight structural materials, thermal insulation materials, flame retardant materials, shock absorbing materials,reinforced framework materials, energy storage devices and aerospace, ad provides a way for studying multifunctional materials.

The invention discloses a preparation method of a DNA hybrid catalyst (G4DNA)/MOFs composite material. The preparation method comprises the following steps: sequentially mixing a guanine (G)-rich single-stranded DNA buffer solution with a potassium chloride (KCl) buffer solution and a hemin buffer solution to obtain a DNA hybrid catalyst (G4DNA); and mixing a certain amount of G4DNA with an aqueous imidazole-2-formaldehyde solution (having a concentration of 200 mM and containing 2 wt% of a stabilizer polyvinylpyrrolidone (PVP)) at room temperature, adding an aqueous solution (with a concentration of 416 mM) of zinc nitrate, and carrying out stirring to obtain the DNA hybrid catalyst/MOFs composite material, namely G4DNA@ZIF-90. The preparation method has the advantages of simple reactionconditions, few synthesis steps, short time consumption and low production cost. The preparation method of the DNA hybrid catalyst/MOFs composite material is used for applying artificial enzyme to thefield of industrial catalysis, and has potential application value.

The invention belongs to the field of preparation of porous intelligent materials, and particularly relates to preparation of an intelligent aerogel in-situ composite system with shape memory performance. In-situ composite regulation and control on the nanoparticles is achieved. The microstructure and the performance of the porous intelligent aerogel are regulated and controlled. The shape memory aerogel in-situ composite material is prepared by using a vacuum freeze-drying-controllable carbonization method, and the technical problems of non-uniform dispersion and poor performance controllability when a nano material is adopted for modifying the surface of a porous intelligent material are solved. The preparation process is good in controllability, in-situ composite modification of the nano material and the porous intelligent material is easy to realize, and the universality is high. The intelligent aerogel in-situ composite system is high in structural designability, good in mechanical property, good in shape memory controllability, low in density and good in hydrophilicity, and has excellent application potential in the fields of intelligent adsorption and detection, environmental energy catalysis, marine resource development and utilization, intelligent gas-sensitive materials, artificial enzymes and the like; and an effective way is provided for developing a porous intelligent composite material.

The invention discloses a high-vitality glutathione peroxidase GPX3 (glutathione peroxidase) mutant and a preparation method thereof, belonging to the field of biotechnologies. With a human GPX3 as a template, through computer simulation and fixed point mutation, a novel high-vitality glutathione peroxidase GPX3 mutant is obtained, wherein the high-vitality glutathione peroxidase GPX3 mutant comprises 207 amino acids; and compared with the human GPX3, the high-vitality glutathione peroxidase GPX3 mutant does not contain cysteine and is higher in GPX activity and better in stability; all cysteines in the human GPX3 are mutated into serines or alanines, but the 54th site is always selenocysteine (SeCys). According to the method, the high-enzyme activity GPX3 mutant protein is directly expressed in a mammal cell strain or auxotroph prokaryotic expression system and an SPP (trisodium phosphate) low-temperature expression system thereof by using a genetic engineering technology. According to the preparation method, a novel extremely high GPX activity artificial enzyme can be prepared without chemical modification. The preparation method disclosed by the invention is wide in application prospect on the aspect of bio-pharmaceuticals.

The invention discloses an artificial G-quadruplex DNA metalloenzyme and application thereof. The metalloenzyme consists of ferriporphyrin and artificial G-quadruplex DNA. In the presence of K < + >, the artificial G-quadruplex DNA and ferriporphyrin are mixed in a buffer solution, and the artificial G-quadruplex DNA metalloenzyme can be obtained through in-situ synthesis by stirring. When the artificial G-quadruplex metalloenzyme is applied to olefin asymmetric cyclopropanation reaction, the artificial G-quadruplex metalloenzyme shows relatively high catalytic activity and relatively high selectivity. The artificial G-quadruplex DNA metalloenzyme does not contain precious metal components, the reaction cost is remarkably reduced, and the artificial G-quadruplex DNA metalloenzyme is mild in use condition and is a novel artificial enzyme which is environmentally friendly, high in activity, high in selectivity and excellent in stability.

The intelligent catalyst consists of vinyl imidazole 20-40 wt%, 2-acrylic amino-2-methyl-1-propyl sulfonic acid 20-40 wt%, and N, N-methylene-diacrylamide 11-22 wt% to form crosslinked polymer compound as well as additive reagent 9-18 wt%. Its preparation includes the steps of: free radical emulsion polymerization of vinyl imidazole, 2-acrylic amino-2-methyl-1-propyl sulfonic acid, and N, N-methylene-diacrylamide to form crosslinked electric response intelligent polymer catalyst; and the test of the catalytic activity. The present invention has the advantages of the flexibility and mobility introduced into the catalyst structure to solve the problem of the polymer catalyst in accessibility and to raise the activity; and the capacity of using the catalyst as artificial enzyme model to simulate the flexibility of enzyme.

The present invention comprises an in vitro enzymatic process that effectively converts renewable polysaccharides into high yields of hydrogen at mild conditions, using only enzymes and water. The process comprises a number of enzymes: (1) phosphorylases, (2) phosphoglucomutases, (3) hydrogenases, and (4) enzymes involved in the pentose-phosphate pathway. Preferred embodiments of the process produce only hydrogen and carbon dioxide as net products, translating into an inexpensive method of generating hydrogen in very large quantities from low-cost feedstocks.