Biohydrogen production by an artificial enzymatic pathway

A hydrogen production, enzymatic technology, applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve the problems of limited, high cost, etc.

Inactive Publication Date: 2009-07-22
VIRGINIA TECH INTPROP INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0016] Existing technologies for producing hydrogen fuel still face many challenges, such as limited yield, high energy input required, high cost, and additional purification steps to ensure that the fuel is sufficiently clean

Method used

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  • Biohydrogen production by an artificial enzymatic pathway
  • Biohydrogen production by an artificial enzymatic pathway
  • Biohydrogen production by an artificial enzymatic pathway

Examples

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Embodiment 1

[0094] Example 1: Enzymatic production of H from starch and water 2 and CO 2

[0095] To illustrate the practical nature of the present methods, systems and compositions, starch was converted to hydrogen and carbon dioxide in a process according to the present invention. Experiments were performed in a continuous flow system equipped with an ice-cooled moisture eliminator. All chemicals and enzymes were purchased from Sigma-Aldrich Co. (U.S.A.) unless otherwise stated. In Table 1 all enzymes and their catalyzed reactions are listed. The working volume of a typical reactor is 2 mL. The system was continuously purged with helium at a flow rate of 50 mL / min. The temperature of the jacketed reactor was maintained at 30°C using a Polyscience (Niles, IL) circulating water bath.

[0096] Table 1: Enzymes and Catalyzed Reactions

[0097] EC Enzyme name reaction source unit 2.4.1.1

glycogen phosphorylase

(C 6 h 10 o 5 )n+Pi+H 2 O →

(C ...

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Abstract

The present invention comprises an in vitro enzymatic process that effectively converts renewable polysaccharides into high yields of hydrogen at mild conditions, using only enzymes and water. The process comprises a number of enzymes: (1) phosphorylases, (2) phosphoglucomutases, (3) hydrogenases, and (4) enzymes involved in the pentose-phosphate pathway. Preferred embodiments of the process produce only hydrogen and carbon dioxide as net products, translating into an inexpensive method of generating hydrogen in very large quantities from low-cost feedstocks.

Description

[0001] Cross References to Related Applications [0002] This application relies on, and claims priority to, U.S. Patent Application 11 / 747,496, filed May 11, 2007, and U.S. Provisional Patent Application 60 / 799,685, filed May 12, 2006, both of which are hereby incorporated The entire content of is incorporated by reference. technical field [0003] The invention relates to biotechnology in the fields of agriculture and energy production. More specifically, the present invention relates to the production of hydrogen from renewable polysaccharides by substantially enzymatically catalyzing the polysaccharides into hydrogen and carbon dioxide. Background technique [0004] Climate change and the eventual depletion of the world's fossil fuel reserves threaten sustainable development (Morris, 2006; Hoffert et al., 2002; Farrell et al., 2006). Hydrogen is a mobile energy carrier that is plentiful, clean, and apparently carbon-free. According to the U.S. Department of Energy (20...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/06C12P3/00
Inventor 珀西瓦尔·毅恒·张乔纳森·米埃伦斯
Owner VIRGINIA TECH INTPROP INC
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