65 results about "Enzymatic process" patented technology

The present invention relates to novel carbamate hydrolases for the enzymatic decomposition of polyurethanes and to an enzymatic process for the complete decomposition of polyurethanes into defined monomers.

The invention relates to microbial colorant compositions and associated processes to produce the same. Microbial colorant compositions are produced by combining microbially derived pigment molecules and microbial biosurfactants. The pigment molecules are obtained either directly from a fermentation broth or by extracting a precursor and transforming it into a pigment using chemical or enzymatic processes. A separately produced biosurfactant, such as a rhamnolipid or surfactin, is added to the obtained pigment molecule to create a pre-dispersed formulation. The biosurfactant may be added at various steps of This approach improves pigment dispersion, solubility, and substrate compatibility, enabling stable aqueous formulations without synthetic additives. The resulting compositions are suitable for wide range of applications including in textiles, food, cosmetics, and polymer materials. Processes for producing and combining the pigment and biosurfactant are also disclosed, including variations in fermentation, extraction, and formulation stages.

The present invention relates to new urethanases for the enzymatic breakdown of polyurethanes and to an enzymatic process for the complete breakdown of polyurethanes into defined monomers.

The present invention refers to a new enzymatic process for obtaining 17α-monoesters of cortexolone and / or its 9,11-dehydroderivatives starting from the corresponding 17α,21-diesters which comprises an enzymatic alcoholysis reaction. Furthermore, the present invention refers to new crystalline forms of cortexolone-17α-propionate and 9,11-dehydro-cortexolone 17α-butanoate.

We are disclosing derivatives of 5-hydroxylated polymethoxyflavones. The derivatives offer enhanced oral bioavailability and improved intestinal permeability. The derivatives of the invention release the biologically active 5-hydroxylated polymethoxyflavones that are activated by chemical or enzymatic processes in the GI Tract. The derivatives are constructed as bioreversible prodrugs containing the bioactive molecules covalently linked with lipids like fatty acids, glycerides or phospholipids. The derivatives are useful against inflammatory conditions and aging. Formulations including the derivatives are also disclosed.

The invention belongs to the crossing field of chemical biology and green catalysis, and particularly relates to application of an artificial photoenzyme as a catalyst to synthesis of an esomeprazole compound. The artificial photoenzyme is constructed by inserting non-natural amino acid containing a benzophenone structure into a protein skeleton; the invention aims to realize in-situ generation and chiral control of singlet oxygen by introducing a benzophenone photosensitive group into a protein skeleton at a fixed point, and solves the technical problems of need of expensive renewable coenzyme, poor enzyme stability, high loading capacity and low yield in the existing esomeprazole enzyme method process.

Verbascum nigrum extracts are disclosed, as well as processes for preparing the same and uses as active ingredients in nutraceuticals. In particular, it is disclosed an enzymatic process wherein a specific enzyme cocktail is used for selectively releasing and extracting compounds from a Verbascum nigrum biomass. It is also disclosed a hydroalcoholic process for releasing and extracting different compounds from a Verbascum nigrum biomass. Said Verbascum nigrum biomass is advantageously obtained from vertical farming, which allows to maximize the stability and properties' reproducibility of the botanical with respect to the corresponding wild-growing plants. The resulting enzymatic and hydroalcoholic extracts can be advantageously used singularly or in combination thereof, thus taking benefit of the overall active compound mixture. Specifically, the extracts can be suitable used in nutraceutical compositions and food supplements.

The application discloses a tricholoma matsutake mushroom and vegetable fresh composite seasoning and belongs to the field of food seasoning processing. The seasoning contains 7-15 parts of edible mushroom composite enzymatic extract, 5-15 parts of laminaria enzymatic extract, 35-45 parts of edible salt and other raw materials in terms of mass fraction; the edible mushroom composite enzymatic extract and the laminaria enzymatic extract are respectively prepared through a two-step enzymatic process, and the edible mushroom contains multiple rare mushroom species such as tricholoma matsutake and lentinus edodes. The seasoning takes rare edible mushroom and laminaria as the umami core, and is compounded with kale powder and fruit and vegetable powder, is rich in flavor and nutrition, and has the functions of seasoning and nutrition supplementing; the umami synergy is realized through the enzymatic process, the umami strength is high, and the taste is mellow; the raw materials are natural and free of chemical synthetic umami agents, the utilization rate of the raw materials is high, the clean label trend is met, the seasoning can be applied to the field of high-end soup bases and the like, and the product has high added value.

An enzymatic process for the separation of isomers of 2,6-dimethyl-2,3-dihydro-1H-inden-1- amine.

To provide an immobilized enzyme composition for hexose production or an improved process for hexose production. [Solution] The present invention relates to an immobilized enzyme composition for the preparation of hexoses. Examples of hexoses include tagatose, psicose, fructose, allose, mannose, galactose, altrose, talose, sorbose, gross, idose, and inositol. The present invention also relates to an enzymatic process for preparing hexoses from sugars by contacting starch derivatives with the immobilized enzyme composition of the present invention.

The application discloses an enzymatic production process of diglyceride, and comprises the following steps: using low-cost lipase as a catalyst, preparing lipase A through immobilization of the enzyme, preparing lipase B through an ester exchange reaction, and then using the lipase B as a catalyst to catalyze esterification of free fatty acid and glycerol to produce diglyceride; the method can realize efficient enzymatic production of diglyceride through a series of methods by using low-cost lipase, and the production cost is reduced. The application provides an economical and efficient method for producing diglyceride by using the enzyme method, greatly reduces the use cost of lipase in the enzymatic esterification reaction, and has certain industrialization application prospect.

The invention relates to novel urethanases for the enzymatic degradation of polyurethanes and to an enzymatic process for the complete degradation of polyurethanes in defined monomers.

This invention relates to the field of textile processing technology, specifically to a method for preparing hydrophobic PET fabrics via an enzymatic process, and the resulting fabric. The invention pre-activates the surface of the PET fabric using an enzymatic method, followed by an amidation reaction with a long-chain fatty amine in the presence of a condensing agent, constructing a covalently fixed alkyl hydrophobic layer. Compared to traditional surface treatments or coatings using alkalis, the method provided by this invention offers milder processing conditions, eliminating the need for large amounts of toxic chemical reagents. Furthermore, the PET hydrolase used in this method exhibits mild action, high selectivity, and high specificity, preferentially attacking the amorphous regions of PET with minimal impact on the crystalline structure and mechanical properties of the PET fibers. This achieves a balance between surface modification and mechanical properties, which is of significant importance for the subsequent functional finishing of PET fibers.

Improved enantioselective enzymatic processes for preparing ursodeoxycholic acid with high pharmaceutical purity are provided. Also provided are improved enantioselective enzymatic processes for preparing ursodeoxycholic acid with high yield and high pharmaceutical and enantiomeric purity.

The present invention relates to lipid compositions derived from insects. The intrinsic chemical / physical properties of insect derived lipids limit the possibilities for widespread and large-scale use. An enzymatic process for the partial hydrolysis and transesterification of insect extracted lipids is provided. The process alters the ratio of triglyceride (TAG), diglycerides (DAG), monoglycerides (MAG) and free fatty acids (FFA) of the crude insect lipid and also results in the transesterification of fatty acids on glyceride backbone yielding a lipid composition that combines an increased dispersibility in water, a more favourable hardness profile (as a function of temperature) and a melting point slightly above ambient temperature. The compositions of the invention further have good nutritive properties as well as some interesting functional properties, e.g. anti-microbial effects. The invention relates to these enzyme-modified lipid compositions, to the methods for producing them, as well as to their uses as an ingredient in an alimentary product.

This invention discloses an enzymatic method for preparing (S)-adenosylmethionine. The method uses adenosine diphosphate (ADP) and L-methionine as substrates, reacting them under the combined action of polyphosphate kinase (Ppk) and adenosylmethionine synthase (MAT) to obtain (S)-adenosylmethionine. This method produces a product with high chiral purity and high atom economy, making it more suitable for industrial production.

The invention relates to an enzymatic process for reducing the lactose content of a galacto-oligosaccharide composition and the production of a high purity galacto-oligosaccharide with a high retention of bifidogenic disaccharides.

An enzymatic process for the separation of isomers of 2,6-dimethyl-2,3-dihydro-lH-inden-l- amine

The invention relates to an enzymatic process for preparing a composition rich in maltotetraose.

The invention relates to the technical field of analysis and testing, and discloses a quality detection method for 6-aminopenicillanic acid, which can effectively detect coporphyrin I which is generated in a penicillin fermentation-enzyme method process and causes the 6-aminopenicillanic acid to become powder, and can be used for detecting the content of porphyrin in each main step of the process. The generation reasons and the effective removal steps are known, so that the technical process control is better guided; meanwhile, the chromatographic conditions can also be used for detecting the content of porphyrin in each main step of the process for producing 6-aminopenicillanic acid so as to understand the generation reasons and effective removal steps of the porphyrin, so that the process control is better guided.

The present invention refers to a new enzymatic process for obtaining 17α-monoesters of cortexolone and / or its 9,11-dehydroderivatives starting from the corresponding 17α,21-diesters which comprises an enzymatic alcoholysis reaction. Furthermore, the present invention refers to now crystalline forms of cortexolone-17α-propionate and 9,11-dehydro-cortexolone 17α-butanoate.

Disclosed herein are methods of producing hexoses from saccharides by enzymatic processes. The methods utilize fructose 6-phosphate and at least one enzymatic step to convert it to a hexose.

The present disclosure relates to methods, kits, and compositions for improving the efficiency of homologous recombination. In particular, the disclosure relates to methods for introducing a nucleic acid cutting entity for DNA editing into cells that are difficult to transfect. Generally, the nucleic acid cutting entity is introduced into the cell without the use of viral vectors. The disclosure also relates to cloning DNA molecules directly into a genome with the combined use of promoter trapping and short homology arms, nuclear localization signal, and / or binding one or more DNA binding agents (TAL effector domain or truncated guide RNA bound by Cas9) to specific sites thereby displacing or restructuring chromatin at the target locus, and / or it increasing the accessibility of the target locus to further enzymatic modifications. The methods and compositions provided herein are, inter alia, useful for genome editing and enhancing enzymatic processes involved therein.

The invention discloses a method for preparing walnut polypeptide by an aqueous enzymatic method. The method comprises the following steps: by taking fresh walnut meal as a raw material, mildly crushing plant cell walls through an aqueous enzymatic method (adding cellulase, pectinase and amylase) to release protein, shearing a protein solution at a high speed, carrying out directional enzymolysis by adopting alkaline protease under the condition of dynamic pH regulation (citric acid or dietary alkali regulation), desalting, and carrying out spray drying to obtain the small-molecular-weight walnut polypeptide. The product obtained by the method is yellowish, has no peculiar smell, and has strong oxidation resistance (for example, the DPPH free radical scavenging rate is greater than or equal to 80%). A traditional chemical wall breaking technology is replaced by an aqueous enzymatic method, strong acid and strong alkali are not needed, the process is green and safe, and the natural color and the active structure are effectively reserved. The embodiment verifies that the walnut polypeptide is excellent in oxidation resistance and sensory quality, the production process is compatible with existing food processing equipment, and the walnut polypeptide has large-scale application potential. During industrial implementation, process parameters (such as enzyme types and reaction time) can be optimized according to enzymolysis efficiency, cost control and terminal application requirements.

The invention belongs to the technical field of edible mushroom waste extraction, and provides a method for extracting mineral substances from edible mushroom waste, which comprises the following steps: drying, crushing and screening mushroom dregs, pressurizing the mushroom dregs under a certain high-pressure condition, extracting and filtering the mushroom dregs by using an extracting solution, and filtering to obtain the mineral substances. Then, step-by-step enzymolysis is carried out through ultrasonic replication under a certain condition, enzyme deactivation is carried out, then centrifugal treatment is carried out, supernate is obtained, and then the supernate is purified and dried, so that a final extract can be obtained. According to the method, the mushroom dregs are pretreated under the high-pressure condition, the degradation efficiency of the mushroom dregs can be improved, active ingredients in the mushroom dregs are fully released, the yield of subsequent mushroom dreg extracts is increased, the whole enzymolysis process is assisted by ultrasonic waves, extraction of nutrient substances in the mushroom dregs is enhanced, the heat transfer and mass transfer process is accelerated, and the quality of the mushroom dregs is improved. The yield of the mushroom dreg extract is further improved.

This invention provides a method and application for preparing biological dressings using a combination of enzymes. The preparation method includes static water thawing; dehairing and degreasing; cutting and segmenting; fine trimming and perforation; iodine immersion treatment; purified water immersion treatment; trypsin and neutral protease solution immersion treatment; purified water immersion treatment; antibacterial coating treatment; packaging; and irradiation. This invention significantly improves the antigen removal rate through the combined use of two enzymes. The combined use of these two enzymes enables comprehensive and efficient degradation of different types of heterogeneous proteins, forming a complementary enzymatic process. The combined use of two enzymes can greatly increase the antigen removal rate, significantly improve the biocompatibility of the biological dressing, reduce the risk of immune rejection in clinical applications, and the prepared biological dressing has good biocompatibility and suitable clinical applicability.

The invention relates to a novel process for generating N-acetylgalactosamine (GalNAc) clusters using a nitrilase and conjugating said GalNAc clusters to oligonucleotides, such as oligonucleotides for use in therapy. In particular, the nitrilase is used to catalyse the conversion of a trinitrile intermediate to a triacid intermediate.

The present invention relates to novel urethanases for the enzymatic degradation of polyurethanes and to an enzymatic process for the complete degradation of polyurethanes in defined monomers.

The utility model relates to the technical field of low-carbon maltodextrin, and discloses a low-carbon maltodextrin preparation device based on an enzymatic process, which comprises a reaction kettle, a support frame fixedly mounted on the upper surface of the reaction kettle, a screening frame fixedly mounted on the upper surface of the support frame, and a fixed plate fixedly mounted on the inner wall of the screening frame, a spring is fixedly mounted on the upper surface of the fixed plate, a filter plate is fixedly mounted on the upper surface of the spring, a vibrator is fixedly mounted on the lower surface of the filter plate, a feeding barrel is fixedly mounted on the upper surface of the reaction kettle, and a spraying head is fixedly mounted on the inner wall of the feeding barrel; through cooperative arrangement of the supporting frame, the screening frame, the fixing plate, the spring, the filter plate, the vibrator, the feeding barrel, the spraying head, the conveying pipe, the material box and the water inlet, the effect of filtering raw materials is achieved, the situation that the reaction is not uniform due to caking in the raw materials is avoided, the contact uniformity of enzyme and the raw materials is ensured to a certain extent, and the production quality is improved.

The present application relates to the field of flavor technology, in particular to a sweet potato roasting flavor preparation method based on starch enzymatic monitoring, product and application, comprising the following steps: step S1, monitoring the starch enzymatic process of sweet potato, determining the maltose generation amount in the starch enzymatic stage of sweet potato roasting process; step S2, separating effective components of sweet potato, retaining sweet potato water extract and sweet potato peel treatment; step S3, constructing a simulated reaction system, combining sweet potato water extract and sweet potato peel treatment, adding maltose to the system, constructing a simulated reaction system, and concentrating into a paste; step S4, simulating sweet potato roasting, microwave roasting the paste, and obtaining sweet potato roasting flavor after diluting the roasting reaction product. The present application significantly improves the fidelity and richness of sweet potato roasting flavor, solves the problem that the sweet potato roasting flavor prepared by the existing preparation method is obviously different from the real sweet potato roasting aroma, and the production process is simple, the product yield is higher, and it is more suitable for industrial production.