43 results about "Enzymatic process" patented technology

The invention relates to microbial colorant compositions and associated processes to produce the same. Microbial colorant compositions are produced by combining microbially derived pigment molecules and microbial biosurfactants. The pigment molecules are obtained either directly from a fermentation broth or by extracting a precursor and transforming it into a pigment using chemical or enzymatic processes. A separately produced biosurfactant, such as a rhamnolipid or surfactin, is added to the obtained pigment molecule to create a pre-dispersed formulation. The biosurfactant may be added at various steps of This approach improves pigment dispersion, solubility, and substrate compatibility, enabling stable aqueous formulations without synthetic additives. The resulting compositions are suitable for wide range of applications including in textiles, food, cosmetics, and polymer materials. Processes for producing and combining the pigment and biosurfactant are also disclosed, including variations in fermentation, extraction, and formulation stages.

We are disclosing derivatives of 5-hydroxylated polymethoxyflavones. The derivatives offer enhanced oral bioavailability and improved intestinal permeability. The derivatives of the invention release the biologically active 5-hydroxylated polymethoxyflavones that are activated by chemical or enzymatic processes in the GI Tract. The derivatives are constructed as bioreversible prodrugs containing the bioactive molecules covalently linked with lipids like fatty acids, glycerides or phospholipids. The derivatives are useful against inflammatory conditions and aging. Formulations including the derivatives are also disclosed.

The application discloses a tricholoma matsutake mushroom and vegetable fresh composite seasoning and belongs to the field of food seasoning processing. The seasoning contains 7-15 parts of edible mushroom composite enzymatic extract, 5-15 parts of laminaria enzymatic extract, 35-45 parts of edible salt and other raw materials in terms of mass fraction; the edible mushroom composite enzymatic extract and the laminaria enzymatic extract are respectively prepared through a two-step enzymatic process, and the edible mushroom contains multiple rare mushroom species such as tricholoma matsutake and lentinus edodes. The seasoning takes rare edible mushroom and laminaria as the umami core, and is compounded with kale powder and fruit and vegetable powder, is rich in flavor and nutrition, and has the functions of seasoning and nutrition supplementing; the umami synergy is realized through the enzymatic process, the umami strength is high, and the taste is mellow; the raw materials are natural and free of chemical synthetic umami agents, the utilization rate of the raw materials is high, the clean label trend is met, the seasoning can be applied to the field of high-end soup bases and the like, and the product has high added value.

An enzymatic process for the separation of isomers of 2,6-dimethyl-2,3-dihydro-1H-inden-1- amine.

To provide an immobilized enzyme composition for hexose production or an improved process for hexose production. [Solution] The present invention relates to an immobilized enzyme composition for the preparation of hexoses. Examples of hexoses include tagatose, psicose, fructose, allose, mannose, galactose, altrose, talose, sorbose, gross, idose, and inositol. The present invention also relates to an enzymatic process for preparing hexoses from sugars by contacting starch derivatives with the immobilized enzyme composition of the present invention.

The application discloses an enzymatic production process of diglyceride, and comprises the following steps: using low-cost lipase as a catalyst, preparing lipase A through immobilization of the enzyme, preparing lipase B through an ester exchange reaction, and then using the lipase B as a catalyst to catalyze esterification of free fatty acid and glycerol to produce diglyceride; the method can realize efficient enzymatic production of diglyceride through a series of methods by using low-cost lipase, and the production cost is reduced. The application provides an economical and efficient method for producing diglyceride by using the enzyme method, greatly reduces the use cost of lipase in the enzymatic esterification reaction, and has certain industrialization application prospect.

The invention relates to novel urethanases for the enzymatic degradation of polyurethanes and to an enzymatic process for the complete degradation of polyurethanes in defined monomers.

This invention relates to the field of textile processing technology, specifically to a method for preparing hydrophobic PET fabrics via an enzymatic process, and the resulting fabric. The invention pre-activates the surface of the PET fabric using an enzymatic method, followed by an amidation reaction with a long-chain fatty amine in the presence of a condensing agent, constructing a covalently fixed alkyl hydrophobic layer. Compared to traditional surface treatments or coatings using alkalis, the method provided by this invention offers milder processing conditions, eliminating the need for large amounts of toxic chemical reagents. Furthermore, the PET hydrolase used in this method exhibits mild action, high selectivity, and high specificity, preferentially attacking the amorphous regions of PET with minimal impact on the crystalline structure and mechanical properties of the PET fibers. This achieves a balance between surface modification and mechanical properties, which is of significant importance for the subsequent functional finishing of PET fibers.

Improved enantioselective enzymatic processes for preparing ursodeoxycholic acid with high pharmaceutical purity are provided. Also provided are improved enantioselective enzymatic processes for preparing ursodeoxycholic acid with high yield and high pharmaceutical and enantiomeric purity.

This invention discloses an enzymatic method for preparing (S)-adenosylmethionine. The method uses adenosine diphosphate (ADP) and L-methionine as substrates, reacting them under the combined action of polyphosphate kinase (Ppk) and adenosylmethionine synthase (MAT) to obtain (S)-adenosylmethionine. This method produces a product with high chiral purity and high atom economy, making it more suitable for industrial production.

An enzymatic process for the separation of isomers of 2,6-dimethyl-2,3-dihydro-lH-inden-l- amine

The invention relates to an enzymatic process for preparing a composition rich in maltotetraose.

The invention relates to the technical field of analysis and testing, and discloses a quality detection method for 6-aminopenicillanic acid, which can effectively detect coporphyrin I which is generated in a penicillin fermentation-enzyme method process and causes the 6-aminopenicillanic acid to become powder, and can be used for detecting the content of porphyrin in each main step of the process. The generation reasons and the effective removal steps are known, so that the technical process control is better guided; meanwhile, the chromatographic conditions can also be used for detecting the content of porphyrin in each main step of the process for producing 6-aminopenicillanic acid so as to understand the generation reasons and effective removal steps of the porphyrin, so that the process control is better guided.

The present invention refers to a new enzymatic process for obtaining 17α-monoesters of cortexolone and / or its 9,11-dehydroderivatives starting from the corresponding 17α,21-diesters which comprises an enzymatic alcoholysis reaction. Furthermore, the present invention refers to now crystalline forms of cortexolone-17α-propionate and 9,11-dehydro-cortexolone 17α-butanoate.

Disclosed herein are methods of producing hexoses from saccharides by enzymatic processes. The methods utilize fructose 6-phosphate and at least one enzymatic step to convert it to a hexose.

The invention discloses a method for preparing walnut polypeptide by an aqueous enzymatic method. The method comprises the following steps: by taking fresh walnut meal as a raw material, mildly crushing plant cell walls through an aqueous enzymatic method (adding cellulase, pectinase and amylase) to release protein, shearing a protein solution at a high speed, carrying out directional enzymolysis by adopting alkaline protease under the condition of dynamic pH regulation (citric acid or dietary alkali regulation), desalting, and carrying out spray drying to obtain the small-molecular-weight walnut polypeptide. The product obtained by the method is yellowish, has no peculiar smell, and has strong oxidation resistance (for example, the DPPH free radical scavenging rate is greater than or equal to 80%). A traditional chemical wall breaking technology is replaced by an aqueous enzymatic method, strong acid and strong alkali are not needed, the process is green and safe, and the natural color and the active structure are effectively reserved. The embodiment verifies that the walnut polypeptide is excellent in oxidation resistance and sensory quality, the production process is compatible with existing food processing equipment, and the walnut polypeptide has large-scale application potential. During industrial implementation, process parameters (such as enzyme types and reaction time) can be optimized according to enzymolysis efficiency, cost control and terminal application requirements.

This invention provides a method and application for preparing biological dressings using a combination of enzymes. The preparation method includes static water thawing; dehairing and degreasing; cutting and segmenting; fine trimming and perforation; iodine immersion treatment; purified water immersion treatment; trypsin and neutral protease solution immersion treatment; purified water immersion treatment; antibacterial coating treatment; packaging; and irradiation. This invention significantly improves the antigen removal rate through the combined use of two enzymes. The combined use of these two enzymes enables comprehensive and efficient degradation of different types of heterogeneous proteins, forming a complementary enzymatic process. The combined use of two enzymes can greatly increase the antigen removal rate, significantly improve the biocompatibility of the biological dressing, reduce the risk of immune rejection in clinical applications, and the prepared biological dressing has good biocompatibility and suitable clinical applicability.

The invention relates to a novel process for generating N-acetylgalactosamine (GalNAc) clusters using a nitrilase and conjugating said GalNAc clusters to oligonucleotides, such as oligonucleotides for use in therapy. In particular, the nitrilase is used to catalyse the conversion of a trinitrile intermediate to a triacid intermediate.

The utility model relates to the technical field of low-carbon maltodextrin, and discloses a low-carbon maltodextrin preparation device based on an enzymatic process, which comprises a reaction kettle, a support frame fixedly mounted on the upper surface of the reaction kettle, a screening frame fixedly mounted on the upper surface of the support frame, and a fixed plate fixedly mounted on the inner wall of the screening frame, a spring is fixedly mounted on the upper surface of the fixed plate, a filter plate is fixedly mounted on the upper surface of the spring, a vibrator is fixedly mounted on the lower surface of the filter plate, a feeding barrel is fixedly mounted on the upper surface of the reaction kettle, and a spraying head is fixedly mounted on the inner wall of the feeding barrel; through cooperative arrangement of the supporting frame, the screening frame, the fixing plate, the spring, the filter plate, the vibrator, the feeding barrel, the spraying head, the conveying pipe, the material box and the water inlet, the effect of filtering raw materials is achieved, the situation that the reaction is not uniform due to caking in the raw materials is avoided, the contact uniformity of enzyme and the raw materials is ensured to a certain extent, and the production quality is improved.

The present application relates to the field of flavor technology, in particular to a sweet potato roasting flavor preparation method based on starch enzymatic monitoring, product and application, comprising the following steps: step S1, monitoring the starch enzymatic process of sweet potato, determining the maltose generation amount in the starch enzymatic stage of sweet potato roasting process; step S2, separating effective components of sweet potato, retaining sweet potato water extract and sweet potato peel treatment; step S3, constructing a simulated reaction system, combining sweet potato water extract and sweet potato peel treatment, adding maltose to the system, constructing a simulated reaction system, and concentrating into a paste; step S4, simulating sweet potato roasting, microwave roasting the paste, and obtaining sweet potato roasting flavor after diluting the roasting reaction product. The present application significantly improves the fidelity and richness of sweet potato roasting flavor, solves the problem that the sweet potato roasting flavor prepared by the existing preparation method is obviously different from the real sweet potato roasting aroma, and the production process is simple, the product yield is higher, and it is more suitable for industrial production.

The invention discloses a method for producing chicken bone soup sauce through an enzymolysis process, and belongs to the technical field of enzyme engineering. Chicken skeletons are used as raw materials, raw bones are directly crushed, high-temperature heat treatment is avoided, 1% flavourzyme is adopted for enzymolysis at 50 DEG C for 2 h, and the hydrolysis degree reaches 44.1%. The product is rich in umami peptide, low in bitter amino acid content and mellow in umami, can realize high-value utilization of chicken bone byproducts, and is suitable for industrial production.

The invention relates to the technical field of enzymolysis yeast milk, and discloses an enzymolysis process of yeast milk based on surfactant-assisted enzymolysis, which comprises the following steps: adding a surfactant glycerol polyoxyethylene polyoxypropylene ether into autolysis yeast milk, stirring and activating, adding cell wall lytic enzyme, carrying out enzymolysis process, then adding helicase, and carrying out enzymolysis to obtain the yeast milk. And finally, papain is added to carry out the enzymolysis process, so that the yeast milk subjected to enzymolysis is obtained. According to the method, a process strategy of combining an enzymolysis method and a surfactant-assisted enzymolysis method is creatively adopted, the accessibility of a substrate is enhanced by utilizing a surfactant glycerol polyoxyethylene polyoxypropylene ether, the contact efficiency of enzyme and the substrate is further improved, the hydrolysis reaction rate is increased, and the comprehensive production cost is effectively reduced while the production efficiency is improved.

The invention relates to the technical field of spices, in particular to a baked sweet potato spice preparation method based on starch enzymolysis monitoring, a product and application, and the baked sweet potato spice preparation method comprises the following steps: S1, monitoring a sweet potato starch enzymolysis process, and measuring the generation amount of maltose in the starch enzymolysis stage in a sweet potato baking process; s2, sweet potato effective components are separated, and sweet potato water extracts and sweet potato peel treatment substances are reserved; s3, constructing a simulated reaction system, namely combining the sweet potato water extract and the sweet potato peel treatment substance, adding maltose into the system, constructing the simulated reaction system, and concentrating into paste; and S4, simulating sweet potato baking, performing microwave baking on the paste, and diluting a baking reaction product to obtain the baked sweet potato spice. According to the preparation method, the fidelity and the richness of the roasted sweet potato spice are remarkably improved, the problem that the roasted sweet potato spice prepared by the existing preparation method is obviously different from the real roasted sweet potato fragrance is solved, the production process is simple and convenient, the product yield is higher, and the preparation method is more suitable for industrial production.

The present invention belongs to the technical field of biology, and particularly relates to a compound for treating myocardial infarction. A chondroitin sulfate oligosaccharide in the present invention is extracted from natural chondroitin sulfate. After the chondroitin sulfate oligosaccharide is treated by a chemically enzymatic process into an oligosaccharide chain, experiments prove that the chondroitin sulfate oligosaccharide contributes to the survival of cardiomyocytes. Compared with other products with similar functions, a glycopeptide polymer hydrogel formed after the introduction of a polymer backbone overcomes the problem of batch-to-batch differences of natural products and has a definite structure. Moreover, the hydrogel has excellent inherent biological activity, and does not need to load active ingredients such as other drugs. The hydrogel exhibits an obvious effect on supporting the ventricular wall in an infarcted area and promoting the survival of cardiomyocytes, and can effectively limit the area of myocardial infarction, increase the ventricular wall thickness of the infarcted area, and alleviate cardiac damage caused by myocardial infarction, and therefore has a good application prospect.

An enzymatic process for the preparation of C16 alkyl polyglycosides and / or C18 alkyl polyglycosides by reacting C16 alkyl glycoside and / or C18 alkyl glycoside with a glycosyl donor containing monosaccharide residues to form an alkyl polyglycoside intermediate which can be fractionated to form an alkyl polyglycoside product, wherein the mole-average degree of polymerization mean DP) of the glycoside chains is greater than or equal to 3.0 units and the molar concentration of alkyl triglycoside (DP3) is greater than alkyl monoglycoside (DPI). The C16 / C18 alkyl polyglycoside product is particularly useful in health care formulations, especially in combination with and / or as a solubilizer for active pharmaceutical ingredients (APIs).

The application discloses an alpha-amino acid ester acyltransferase and a production method of valine-tryptophan dipeptide, and relates to the technical field of biology. The amino acid sequence of the alpha-amino acid ester acyltransferase is shown in SEQ ID NO. 4. By designing and modifying the alpha-amino acid ester acyltransferase, the application successfully obtains a mutant with significantly improved catalytic performance, and establishes an efficient and green enzymatic synthesis process of valine-tryptophan dipeptide. The process is carried out under mild conditions, L-valine methyl ester and L-tryptophan are used as substrates, and the mutant enzyme expressed by the engineering bacteria is used for catalysis, so that the yield of valine-tryptophan dipeptide reaches 22.42 g / L, which is 1.62 times of the original enzyme. The process effectively overcomes the shortcomings of traditional chemical synthesis and existing enzymatic process, has the outstanding advantages of simple steps, mild conditions, high product concentration and environmental friendliness, and provides a reliable and promising technical scheme for the industrial production of valine-tryptophan dipeptide.

An enzymatic process and system are disclosed for producing homogeneous chain length poly hydroxyalkanoate monomers and / or polymers having a chain length of at least eight carbons from a long-chain fatty acid or precursor thereof. The process also results in specific homogenous chain length polyhydroxyalkanoate monomers and / or polymers. The long-chain fatty acid or precursor thereof can be contacted in vitro with an enzyme or a mixture of enzymes to produce the homogeneous chain length polyhydroxyalkanoate.

The present invention relates to a continuous enzymatic process for obtaining virgin oil and a solid by-product rich in proteins, fibres and hydrolysed carbohydrates from coconut pulp, which comprises the steps: dry grinding of the coconut pulp; formation of an aqueous emulsion by mixing the ground pulp with water; wet grinding of the aqueous emulsion; continuous enzymatic hydrolysis in a jacketed, multi-stage continuously stirred tank reactor; centrifugation of the product obtained in the previous step; separation of the mass obtained in the previous step into three fractions: a fraction (1) comprising the supernatant oil, a fraction (2) comprising the aqueous fraction and a fraction (3) comprising the insoluble part; removal of moisture from fraction (1) to a water value of less than 0.1%, obtaining virgin oil; removal of moisture from fraction (3) to a water value of less than 0.1%, obtaining the solid by-product rich in proteins, fibres and hydrolysed carbohydrates; and recovery of the enzyme from fraction (2).

The invention discloses a production method of alpha-amino acid ester acyltransferase and valine dipeptide, and relates to the technical field of biology. The amino acid sequence of the alpha-amino acid ester acyltransferase is as shown in SEQ ID NO. 4. Alpha is modified through design; according to the present invention, the mutant with the significantly improved catalytic performance is successfully obtained, and the efficient and green enzyme-method valine dipeptide synthesis process is established. The process is carried out under mild conditions, L-valine methyl ester and L-tryptophan are taken as substrates, and high-activity mutant enzyme expressed by engineering bacteria is utilized for catalysis, so that the yield of the valine-tryptophan dipeptide is up to 22.42 g / L, which is 1.62 times that of original enzyme, and the defects of traditional chemical synthesis and existing enzymatic process are effectively overcome; the method has the outstanding advantages of simple steps, mild conditions, high product concentration, environmental friendliness and the like, and provides a reliable technical scheme with wide prospects for industrial production of valine dipeptide.

The invention discloses hippophae rhamnoides enzymolysis polysaccharide as well as a preparation method and application thereof. The enzymolysis process conditions of the method are as follows: in a buffer system with the pH value of 5.0, the hippophae rhamnoides crude polysaccharide is degraded under the conditions that the addition amount of polygalacturonic acid-pectinase is 810.6 U / g, the reaction temperature is 51.3 DEG C and the enzymolysis time is 90.1 min. Structural characterization results show that the hippophae rhamnoides polysaccharide after enzymolysis is in a loose and porous form, and the specific surface area is obviously increased; and the molecular weight is greatly reduced from 1.57 * 10 Da to 4.45 * 10 Da. In-vitro activity evaluation shows that the scavenging capacity of the enzymolysis polysaccharide on DPPH free radicals is obviously superior to that of a non-enzymolysis sample; in a non-alcoholic fatty liver model constructed by HepG2 cells, the enzymolysis polysaccharide can effectively reduce the content of triglyceride and total cholesterol in the cells.