83 results about "Enzymatic process" patented technology

The present invention relates to novel carbamate hydrolases for the enzymatic decomposition of polyurethanes and to an enzymatic process for the complete decomposition of polyurethanes into defined monomers.

The invention relates to microbial colorant compositions and associated processes to produce the same. Microbial colorant compositions are produced by combining microbially derived pigment molecules and microbial biosurfactants. The pigment molecules are obtained either directly from a fermentation broth or by extracting a precursor and transforming it into a pigment using chemical or enzymatic processes. A separately produced biosurfactant, such as a rhamnolipid or surfactin, is added to the obtained pigment molecule to create a pre-dispersed formulation. The biosurfactant may be added at various steps of This approach improves pigment dispersion, solubility, and substrate compatibility, enabling stable aqueous formulations without synthetic additives. The resulting compositions are suitable for wide range of applications including in textiles, food, cosmetics, and polymer materials. Processes for producing and combining the pigment and biosurfactant are also disclosed, including variations in fermentation, extraction, and formulation stages.

The invention provides a quality control method for synthesizing 3 '-sialic acid lactose by an enzymatic process, which comprises the following steps: A) dissolving and extracting a test sample by using a solvent to obtain a to-be-detected solution; the test sample is a reaction solution or a finished product of 3 '-sialic acid lactose synthesized by enzyme catalysis; b) determining the liquid to be detected by adopting a high performance liquid chromatography to obtain an HPLC (High Performance Liquid Chromatography) spectrum of the 3 '-sialic acid lactose; according to the method, an HPLC-UV-RI combined detection method is adopted, the specificity is high, the sensitivity is high, the linear correlation is good, and the pretreatment process of the sample is simple and rapid; according to the method, the content of 3 '-lactulose sialylate, 6'-lactose sialylate sodium salt, D-lactose, CMP, CMP-SA and SA in the 3 '-SL process development process can be accurately detected, control over the conversion rate and impurities in the process is facilitated, effective detection of a product 3'-SL related substance-isomer can be achieved, and control over the product quality is facilitated.

The present invention relates to new urethanases for the enzymatic breakdown of polyurethanes and to an enzymatic process for the complete breakdown of polyurethanes into defined monomers.

The present invention refers to a new enzymatic process for obtaining 17α-monoesters of cortexolone and / or its 9,11-dehydroderivatives starting from the corresponding 17α,21-diesters which comprises an enzymatic alcoholysis reaction. Furthermore, the present invention refers to new crystalline forms of cortexolone-17α-propionate and 9,11-dehydro-cortexolone 17α-butanoate.

We are disclosing derivatives of 5-hydroxylated polymethoxyflavones. The derivatives offer enhanced oral bioavailability and improved intestinal permeability. The derivatives of the invention release the biologically active 5-hydroxylated polymethoxyflavones that are activated by chemical or enzymatic processes in the GI Tract. The derivatives are constructed as bioreversible prodrugs containing the bioactive molecules covalently linked with lipids like fatty acids, glycerides or phospholipids. The derivatives are useful against inflammatory conditions and aging. Formulations including the derivatives are also disclosed.

The invention belongs to the crossing field of chemical biology and green catalysis, and particularly relates to application of an artificial photoenzyme as a catalyst to synthesis of an esomeprazole compound. The artificial photoenzyme is constructed by inserting non-natural amino acid containing a benzophenone structure into a protein skeleton; the invention aims to realize in-situ generation and chiral control of singlet oxygen by introducing a benzophenone photosensitive group into a protein skeleton at a fixed point, and solves the technical problems of need of expensive renewable coenzyme, poor enzyme stability, high loading capacity and low yield in the existing esomeprazole enzyme method process.

Verbascum nigrum extracts are disclosed, as well as processes for preparing the same and uses as active ingredients in nutraceuticals. In particular, it is disclosed an enzymatic process wherein a specific enzyme cocktail is used for selectively releasing and extracting compounds from a Verbascum nigrum biomass. It is also disclosed a hydroalcoholic process for releasing and extracting different compounds from a Verbascum nigrum biomass. Said Verbascum nigrum biomass is advantageously obtained from vertical farming, which allows to maximize the stability and properties' reproducibility of the botanical with respect to the corresponding wild-growing plants. The resulting enzymatic and hydroalcoholic extracts can be advantageously used singularly or in combination thereof, thus taking benefit of the overall active compound mixture. Specifically, the extracts can be suitable used in nutraceutical compositions and food supplements.

The present disclosure relates to methods, kits and compositions for improving the efficiency of homologous recombination. Specifically, the invention provides a method. The present disclosure relates to cloning DNA molecules directly into the genome and / or binding one or more DNA binding agents (TAL effector domain to which Cas9 binds or truncated guide RNA) to specific sites by using promoter capture in combination with short homologous arm, nuclear localization signals, methods whereby replacement or reconstitution of chromatin at a target locus and / or to thereby enhance the accessibility of a target locus for further enzymatic modification. The methods and compositions provided herein are particularly useful for genome editing and enhancing the enzymatic processes involved therein.

The application discloses a tricholoma matsutake mushroom and vegetable fresh composite seasoning and belongs to the field of food seasoning processing. The seasoning contains 7-15 parts of edible mushroom composite enzymatic extract, 5-15 parts of laminaria enzymatic extract, 35-45 parts of edible salt and other raw materials in terms of mass fraction; the edible mushroom composite enzymatic extract and the laminaria enzymatic extract are respectively prepared through a two-step enzymatic process, and the edible mushroom contains multiple rare mushroom species such as tricholoma matsutake and lentinus edodes. The seasoning takes rare edible mushroom and laminaria as the umami core, and is compounded with kale powder and fruit and vegetable powder, is rich in flavor and nutrition, and has the functions of seasoning and nutrition supplementing; the umami synergy is realized through the enzymatic process, the umami strength is high, and the taste is mellow; the raw materials are natural and free of chemical synthetic umami agents, the utilization rate of the raw materials is high, the clean label trend is met, the seasoning can be applied to the field of high-end soup bases and the like, and the product has high added value.

An enzymatic process for the separation of isomers of 2,6-dimethyl-2,3-dihydro-1H-inden-1- amine.

To provide an immobilized enzyme composition for hexose production or an improved process for hexose production. [Solution] The present invention relates to an immobilized enzyme composition for the preparation of hexoses. Examples of hexoses include tagatose, psicose, fructose, allose, mannose, galactose, altrose, talose, sorbose, gross, idose, and inositol. The present invention also relates to an enzymatic process for preparing hexoses from sugars by contacting starch derivatives with the immobilized enzyme composition of the present invention.

The application discloses an enzymatic production process of diglyceride, and comprises the following steps: using low-cost lipase as a catalyst, preparing lipase A through immobilization of the enzyme, preparing lipase B through an ester exchange reaction, and then using the lipase B as a catalyst to catalyze esterification of free fatty acid and glycerol to produce diglyceride; the method can realize efficient enzymatic production of diglyceride through a series of methods by using low-cost lipase, and the production cost is reduced. The application provides an economical and efficient method for producing diglyceride by using the enzyme method, greatly reduces the use cost of lipase in the enzymatic esterification reaction, and has certain industrialization application prospect.

The invention relates to novel urethanases for the enzymatic degradation of polyurethanes and to an enzymatic process for the complete degradation of polyurethanes in defined monomers.

This invention relates to the field of textile processing technology, specifically to a method for preparing hydrophobic PET fabrics via an enzymatic process, and the resulting fabric. The invention pre-activates the surface of the PET fabric using an enzymatic method, followed by an amidation reaction with a long-chain fatty amine in the presence of a condensing agent, constructing a covalently fixed alkyl hydrophobic layer. Compared to traditional surface treatments or coatings using alkalis, the method provided by this invention offers milder processing conditions, eliminating the need for large amounts of toxic chemical reagents. Furthermore, the PET hydrolase used in this method exhibits mild action, high selectivity, and high specificity, preferentially attacking the amorphous regions of PET with minimal impact on the crystalline structure and mechanical properties of the PET fibers. This achieves a balance between surface modification and mechanical properties, which is of significant importance for the subsequent functional finishing of PET fibers.

Improved enantioselective enzymatic processes for preparing ursodeoxycholic acid with high pharmaceutical purity are provided. Also provided are improved enantioselective enzymatic processes for preparing ursodeoxycholic acid with high yield and high pharmaceutical and enantiomeric purity.

The present invention relates to lipid compositions derived from insects. The intrinsic chemical / physical properties of insect derived lipids limit the possibilities for widespread and large-scale use. An enzymatic process for the partial hydrolysis and transesterification of insect extracted lipids is provided. The process alters the ratio of triglyceride (TAG), diglycerides (DAG), monoglycerides (MAG) and free fatty acids (FFA) of the crude insect lipid and also results in the transesterification of fatty acids on glyceride backbone yielding a lipid composition that combines an increased dispersibility in water, a more favourable hardness profile (as a function of temperature) and a melting point slightly above ambient temperature. The compositions of the invention further have good nutritive properties as well as some interesting functional properties, e.g. anti-microbial effects. The invention relates to these enzyme-modified lipid compositions, to the methods for producing them, as well as to their uses as an ingredient in an alimentary product.

This invention discloses an enzymatic method for preparing (S)-adenosylmethionine. The method uses adenosine diphosphate (ADP) and L-methionine as substrates, reacting them under the combined action of polyphosphate kinase (Ppk) and adenosylmethionine synthase (MAT) to obtain (S)-adenosylmethionine. This method produces a product with high chiral purity and high atom economy, making it more suitable for industrial production.

The invention relates to an enzymatic process for reducing the lactose content of a galacto-oligosaccharide composition and the production of a high purity galacto-oligosaccharide with a high retention of bifidogenic disaccharides.

An enzymatic process for the separation of isomers of 2,6-dimethyl-2,3-dihydro-lH-inden-l- amine

The invention relates to an enzymatic process for preparing a composition rich in maltotetraose.

The invention relates to the technical field of biology, in particular to an in-vitro two-step enzymatic process for efficiently synthesizing GDP-L-fucose and application. According to the invention, a bifunctional enzyme (Uniprot: Q58T34) with an amino acid sequence as shown in SEQ ID NO: 1 is used as a template to carry out site-directed mutagenesis modification, and a variant M07 with high L-Fucose kinase activity and a variant M10 with high GDP-L-Fucose pyrophosphorylase activity are respectively obtained. The GDP-L-Fucose is prepared from the variant by means of a two-step enzyme method in the presence of FKP enzyme and PPA enzyme through an ATP regeneration system, and the GDP-L-Fucose is high in yield and purity, low in production cost and energy consumption, suitable for large-scale production and wide in application value.

The invention relates to the technical field of analysis and testing, and discloses a quality detection method for 6-aminopenicillanic acid, which can effectively detect coporphyrin I which is generated in a penicillin fermentation-enzyme method process and causes the 6-aminopenicillanic acid to become powder, and can be used for detecting the content of porphyrin in each main step of the process. The generation reasons and the effective removal steps are known, so that the technical process control is better guided; meanwhile, the chromatographic conditions can also be used for detecting the content of porphyrin in each main step of the process for producing 6-aminopenicillanic acid so as to understand the generation reasons and effective removal steps of the porphyrin, so that the process control is better guided.

The present invention relates to a baked product made without any addition of sugar, which still has a suitable sweetness intensity and a good taste. The baked product has a very low content of fructose. The baked product is obtained by a two-step enzymatic process involving a first step of providing sugar from starch, suitable for a fermentation process, followed by a second step of enzymatic hydrolysis of polysaccharides, oligosaccharides and disaccharides, notably to form primarily glucose and maltose. The novel process further allows for an optimized and / or shortened proofing of the dough in the first step.

The present invention refers to a new enzymatic process for obtaining 17α-monoesters of cortexolone and / or its 9,11-dehydroderivatives starting from the corresponding 17α,21-diesters which comprises an enzymatic alcoholysis reaction. Furthermore, the present invention refers to now crystalline forms of cortexolone-17α-propionate and 9,11-dehydro-cortexolone 17α-butanoate.

Disclosed herein are methods of producing hexoses from saccharides by enzymatic processes. The methods utilize fructose 6-phosphate and at least one enzymatic step to convert it to a hexose.

The present disclosure relates to methods, kits, and compositions for improving the efficiency of homologous recombination. In particular, the disclosure relates to methods for introducing a nucleic acid cutting entity for DNA editing into cells that are difficult to transfect. Generally, the nucleic acid cutting entity is introduced into the cell without the use of viral vectors. The disclosure also relates to cloning DNA molecules directly into a genome with the combined use of promoter trapping and short homology arms, nuclear localization signal, and / or binding one or more DNA binding agents (TAL effector domain or truncated guide RNA bound by Cas9) to specific sites thereby displacing or restructuring chromatin at the target locus, and / or it increasing the accessibility of the target locus to further enzymatic modifications. The methods and compositions provided herein are, inter alia, useful for genome editing and enhancing enzymatic processes involved therein.

The present invention relates to a baked product made without any addition of sugar, which still has a suitable sweetness intensity and a good taste. The baked product has a very low content of fructose. The baked product is obtained by a two-step enzymatic process involving a first step of providing sugar from starch, suitable for a fermentation process, followed by a second step of enzymatic hydrolysis of polysaccharides, oligosaccharides and disaccharides, notably to form primarily glucose and maltose. The novel process further allows for an optimized and / or shortened proofing of the dough in the first step.

The present disclosure relates to chemo-enzymatic processes for the preparation of lactones (including, e.g., macrolactones, γ-lactones, and δ-lactones) and / or macrocyclic ketones, which are compounds of industrial value, for example, for use as fragrance ingredients. The chemo-enzymatic processes combine the in vivo microbial production of fatty acid derivatives and the subsequent ex vivo synthetic transformation of the fatty acid derivatives to provide the lactones and macrocyclic ketones.

The invention discloses a method for preparing walnut polypeptide by an aqueous enzymatic method. The method comprises the following steps: by taking fresh walnut meal as a raw material, mildly crushing plant cell walls through an aqueous enzymatic method (adding cellulase, pectinase and amylase) to release protein, shearing a protein solution at a high speed, carrying out directional enzymolysis by adopting alkaline protease under the condition of dynamic pH regulation (citric acid or dietary alkali regulation), desalting, and carrying out spray drying to obtain the small-molecular-weight walnut polypeptide. The product obtained by the method is yellowish, has no peculiar smell, and has strong oxidation resistance (for example, the DPPH free radical scavenging rate is greater than or equal to 80%). A traditional chemical wall breaking technology is replaced by an aqueous enzymatic method, strong acid and strong alkali are not needed, the process is green and safe, and the natural color and the active structure are effectively reserved. The embodiment verifies that the walnut polypeptide is excellent in oxidation resistance and sensory quality, the production process is compatible with existing food processing equipment, and the walnut polypeptide has large-scale application potential. During industrial implementation, process parameters (such as enzyme types and reaction time) can be optimized according to enzymolysis efficiency, cost control and terminal application requirements.