4 results about "Phosphoribosyl transferase" patented technology

The invention relates to recombinant bacteria for producing L-tryptophan as well as a construction method and application of the recombinant bacteria, and belongs to the technical field of genetic engineering. In the recombinant bacterium, a functional amide phosphoribosyltransferase is deleted, or the functional amide phosphoribosyltransferase and 2-imino propionate deaminase are deleted. According to the method disclosed by the invention, a recombinant bacterium for high-yield tryptophan is constructed, and functional amide phosphoribosyltransferase is deleted in the recombinant bacterium, so that more PRPP and glutamine participate in an anabolic pathway of tryptophan, and a culture mode of supplementing threonine can be matched; according to the tryptophan phosphoribosyltransferase, the problem that a tryptophan synthesis route is lack of PRPP and glutamine and the problem that the cell activity is reduced due to the lack of functional amide phosphoribosyltransferase can be solved at the same time; the main amino supply in the purine synthesis original route is changed from glutamine to 2-aminosuccinic acid as a carrier, so that the tryptophan production capacity can be further improved.

The present invention provides a novel marker for genetic recombination in Starmerella bombicola and a method for using the same. The present invention also provides a Starmerella bombicola mutant strain in which the expression of an orotate phosphoribosyltransferase-like protein or a protein equivalent thereto is suppressed or inactivated, a uracil-requiring selection marker consisting of the gene, and a method for producing a Starmerella bombicola transformant using the selection marker.

The present invention relates to a novel compound for inhibiting nicotinamide phosphoribosyl transferase (NamPT), a composition including the same, and various uses thereof.

The present disclosure provides an antibody and a polypeptide that specifically bind to nicotinate phosphoribosyltransferase (NAPRT). Additionally, a nucleic acid encoding such an antibody, an expression vector and a host cell for producing such an antibody, a method for detecting NAPRT in a sample from a subject requiring such an antibody using such an antibody, and a method for diagnosing a subject having cancer or sepsis using such an antibody are provided. Additionally, a composition comprising such an antibody is provided. Additionally, a composition comprising a nicotinamide phosphoribosyltransferase inhibitor (NAMPTi) and a method for treating cancer using NAMPTi after diagnosis using the NAPRT antibody are provided.