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Method for extraction of tea seed oil by ultrasound-assisted enzymatic process

An ultrasonic-assisted enzymatic method and production method, applied in the direction of fat oil/fat production, fat production, edible oil/fat, etc., can solve the problems of high equipment requirements, complicated operation, low extraction rate, etc., and achieve simple operation and cheap equipment Easy to get, the effect of improving oil yield

Inactive Publication Date: 2013-08-21
HEYUAN LVKANG ECOLOGICAL AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the shortcomings of the existing tea seed oil extraction methods, such as low extraction rate, high equipment requirements, and complicated operation, the present invention provides a method for extracting tea seed oil by an ultrasonic-assisted enzymatic method. The method is simple, safe, and high in extraction rate. The required equipment is cheap and easy to obtain, which is very suitable for promotion and application in economically underdeveloped areas

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Weigh 10 kg of tea seeds, put them on a tray after removing the shell, put them in an oven and bake at a high temperature of 80°C for 30 minutes; grind the tea seeds to 40 mesh tea seed powder with a pulverizer, and sweep the tea seed powder Put it into a plastic bucket, and add tap water at a ratio of 1:6; pour the mixture into the pot, boil to inactivate the enzyme for 5 minutes; after cooling, pour it into the cleaning tank of the ultrasonic instrument, and ultrasonicate it at a frequency of 25kHz for 10 minutes; adjust The temperature is 40°C, with NaHCO 3 After adjusting the pH value to 7.0, add alkaline protease at a ratio of 1%, and react for 2 hours; control the temperature at 45°C, and then use citric acid to adjust the pH value to 4.5, then add compound cellulase at a ratio of 3%. React for 2 h; divide into centrifuge tubes, centrifuge at 15,000 r / min for 40 min at room temperature, and take the upper layer of tea seed oil; put it in a vacuum dryer ...

Embodiment 2

[0020] Example 2: Weigh 50 kg of tea seeds, remove the shell and put them on a round bamboo skip, and expose them to the sun for 5 days; use a pulverizer to crush the tea seeds to 30 mesh tea seed powder, sweep the tea seed powder and pour it into the pot , add tap water at a ratio of 1:6; heat to maintain the temperature in the pot at about 70 degrees; pour it into the cleaning tank of the ultrasonic instrument after 10 minutes, and ultrasonicate it at a frequency of 40 kHz for 15 minutes; maintain the temperature in the pot at 45 ℃, with NaHCO 3 Adjust the pH value to 7.0, add alkaline protease at a ratio of 3%, react for 2 hours, then adjust the pH value to 4.5 with citric acid, add compound cellulase at a ratio of 2%, react for 2 hours, and pack into a centrifuge tube; centrifuge at a speed of 20,000 r / min for 20 minutes to take the upper layer of tea seed oil; put it in a vacuum dryer and dry for 30 minutes to obtain the finished tea seed oil.

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Abstract

The invention relates to a method for extraction of tea seed oil by an ultrasound-assisted enzymatic process. The method includes: subjecting tea seeds to hulling, drying and crushing, then mixing the crushed tea seeds with water, conducting a heat treatment to perform enzyme deactivation, then carrying out an ultrasonic treatment, adjusting the pH, then adding alkaline protease and composite cellulose to undergo enzymolysis, conducting centrifugation and taking the upper oil, and performing vacuum drying. Compared with the existing tea oil extraction technologies, the method provided in the invention has the advantages of high oil yield, simple operation, no harm to human body, as well as cheap and easily available equipment, thus being suitable for large scale promotion and application.

Description

technical field [0001] The invention relates to a process method of edible oil processing, in particular to a production method of tea seed oil. Background technique [0002] Tea seed oil, also known as camellia oil, tea tree seed oil, is a pure natural vegetable oil extracted from the fruit of camellia, an economic crop in mountainous areas of our country. Camellia oil is rich in nutrients and does not contain erucic acid, cholesterol, aflatoxin and other harmful substances. After testing, the unsaturated fatty acid in camellia oil is as high as 90%, oleic acid reaches 80-83%, linoleic acid reaches 7-13%, and it is rich in protein and vitamins A, B, D, E, etc., especially its The rich linolenic acid contained in it is necessary for the human body but cannot be synthesized. Camellia oil is a unique woody oil in my country. Its fatty acid composition is similar to that of olive oil, the best vegetable oil recognized in the world. It has the reputation of "Oriental olive oil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/04A23D9/02
Inventor 周理金
Owner HEYUAN LVKANG ECOLOGICAL AGRI TECH CO LTD
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