40 results about "Glutathione Transferases" patented technology

The present invention relates to methods and compositions to treat subjects having cystic fibrosis. These compositions comprise the class of isothiocyanates. Isothiocyanates, absorbed by a cell are conjugated with glutathione GSH by glutathione-s-tranferase (GST). The conjugates are substrates of the multi-drug resistance associated (MRP) / multi-drug resistance (MDR) proteins. These proteins are functionally redundant to the cystic fibrosis transmembrane conductance regulator (CFTR), allowing for the substrate conjugates to be exported from the cell. The export of GSH conjugates restores intracellular and extracellular levels of GSH to normal levels. Normalizing both extracellular and intracellular GSH via the increased conjugation of isothiocyanates with GSH, and subsequent export, can significantly rectify numerous enzymatic processes and correct the pathologies that are typical of patients suffering from cystic fibrosis.

Peptides having general and specific binding affinities for the Src homology region 3 (SH3) domains of proteins are disclosed in the present invention. In particular, SH3 binding peptides have been isolated from three phage-displayed random peptide libraries which had been screened for isolates that bind to bacterial fusion proteins comprising SH3 and glutathione S-transferase (GST). Preferred peptides are disclosed which comprise a core 7-mer sequence (preferably, a consensus motif) and two or more, preferably at least six, additional amino acid residues flanking the core sequence, for a total length of 9, preferably at least 13, amino acid residues and no more than about 45 amino acid residues. Such peptides manifest preferential binding affinities for certain SH3 domains. The preferred peptides exhibit specific binding affinities for the Src-family of proteins. In vitro and in vivo results are presented which demonstrate the biochemical activity of such peptides.

Polyacrylamide-based methods of fabricating surface-bound peptide and protein arrays, the arrays themselves, and a method of using the arrays to detect biomolecules and to measure their concentration, binding affinity, and kinetics are described. Peptides, proteins, fusion proteins, protein complexes, nucleic acids, and the like, are labeled with an acrylic moiety and attached to acrylic-functionalized glass surfaces through a copolymerization with acrylic monomer. The specific attachment of glutathione S-transferase-green fluorescent protein (GST-GFP) fusion protein was more than 7-fold greater than the nonspecific attachment of non-acrylic labeled GST-GFP. Surface-attached GST-GFP (0.32 ng / mm2) was detectable by direct measurement of green fluorescent protein fluorescence and this lower detection limit was reduced to 0.080 ng / mm2 using indirect antibody-based detection. The polyacrylamide-based surface attachment strategy was also used to measure the kinetics of substrate phosphorylation by the kinase c-Src which is encoded by the Rous Sarcoma virus. The surface attachment strategy is applicable to the proteomics field and addresses denaturation and dehydration problems associated with protein microarray development.

A vinca rosea Glutathione S-transferase gene sequences, and it coding product amino acid sequence. It relates to a kind of new gene sequence, characterized in that: this gene is 929bp, at the point of 56bp there is the start coding triplet ATG, 718bp the ending coding triplet TGA, 910bp additional signal polyA. The gene sequence has not any inner gene, 663bp completely disclosed code-reading frame, code 220 amino acid. Nucleotide sequence and Capsicum Chinese gene GST1 has reached 67.7% homeotic source, and Euphorbia esula gene GST 57.7 homeotic source. This invention also provides a method to gain a full length of forced gene. First we enrich the anti-dry expressing gene in a moderate dry condition; then we establish full length genomic library of cDNA and detecting order, obtain the anti-dry related full length forcing gene in most short period. This anti-dry gene straightly links to eukaryotes expressing carrier pCMV plasmid, detected of the order by t3, t7 primer, filtered by eukaryotes expressing. This invention distills the full length gene of glucoside peptide transferase. This gene has a good anti-oxidate function, increases express in adverse circumstance forces in the process, the gene eliminates the free radical of oxidation. This gene colon expands the gene source of plant anti-dry, and provides a more rich and good usable gene for enhancing the anti-dry function and meliorating breed.

The invention discloses a gene product detection method of degradative graminearum toxin. A rabbit anti-GST(glutathione transferase)-Tri101 antibody acting as a primary antibody, and a goat anti-rabbit IgG-HRP (horse radish peroxidase) acting as a secondary antibody and an object to be tested containing the Tri101 protein carry out Western blot analysis. The trichothecene 3-O-acetyl invertase coded by Tri101 gene in the invention is efficiently expressed in colibacillus; a purified protein immune New Zealand white rabbit obtains an antibody with relatively good specificity, and can carry out immunoblotting reaction with Tri101 protein secreted and expressed in BL21 (DE3); and a new detection method is provided for trangenosis of Tri101 gene in wheat and corns; the detection cost is reduced; and a solid basis for further researching the biological functions of Tri101 protein is provided.

The present invention relates to a method for analyzing residual agricultural chemicals which comprises the steps of acting a reduced glutathione as a reactive substrate and a glutathione transferase serving as a catalyst for the reaction on a carbofuran derivative or a methomyl derivative as a carbamate type agricultural chemical of a new series to thus derivatize the agricultural chemical into a substance having a high choline esterase-inhibitory activity; reacting the substances formed through the derivatization reaction with a choline esterase; and then detecting the presence of the agricultural chemical as the new series of carbamate type one included in a sample to be examined on the basis of the changes in the choline esterase activity thus detected. The method of the present invention may serve as a powerful tool for the detection of the residual agricultural chemicals in grains such as rice and the detection of the content of agricultural chemicals remaining in agricultural products such as vegetables and fruits.

The invention relates to a gene expression product BLSJ-1 with Brucella diagnosis and identification effect, and a preparation method thereof. According to the preparation method, the gene expression product is obtained via gene prokaryotic expression of Brucella S2 strain with GI of 489054867, and via glutathione S transferase (GST) affinity chromatographic column and glutathione gradient elution purifying. The gene expression product can be taken as a coating antigen, and as a diagnosis antigen of animal Brucella vaccine immunity and naturally infected serum detection. When the BLSJ-1 antigen is subjected to western blot with vaccine immune antibodies and natural infection antibodies, the difference is obvious. The gene expression product BLSJ-1 is taken as an indirect enzyme-linked immune adsorption assay (ELISA) coating antigen to detect hundreds of brucellosis clinical serum samples, and differential diagnosis effect is excellent.

The invention discloses a method for high-throughput screening of single-domain antibodies using isPLA. The steps are as follows: constructing an sdAb library containing 21 random amino acid sequences in the CDR3 region, and fusing a 3×Flag tag to the C-terminus of the sequence; co-transfection Cells, fixed cells for isPLA; followed by sorting, amplification, and recombination, and after multiple rounds of screening and sequencing, constructs that sequentially contain glutathione S-transferase GST, tobacco mosaic virus TEV protease cleavage site, and recognize SQSTM1. The sdAb sequence, the translocation domain of Pseudomonas exotoxin ETA and the 3×Flag-tagged recombinant protein were expressed in E. coli, followed by purification and enzyme digestion. The method can screen sdAbs that specifically recognize different antigenic determinants from the constructed high-capacity sdAb library, and has low cost and high efficiency. No preparation of animal samples or hybridomas is involved.

The present invention relates to a novel method for detoxification of trichothecene contaminated materials. More specifically, the present invention relates to a method of bioconverting trichothecene by contacting a material contaminated with trichothecene with an exogenous non-animal glutathione-S-transferase (GST) having substrate specificity for the epoxy ring of trichothecene. The invention also relates to recombinant GST as well as to transgenic plants and animals expressing said GST.

The invention relates to a gene expression product BLSJ-3 with Brucella diagnosis and identification effect, and a preparation method thereof. According to the preparation method, the gene expression product is obtained via gene prokaryotic expression of Brucella S2 strain with GI of 490819668, and via glutathione S transferase (GST) affinity chromatographic column and glutathione gradient elution purifying. The gene expression product can be taken as a coating antigen, and as a diagnosis antigen of animal Brucella vaccine immunity and naturally infected serum detection. When the BLSJ-3 antigen is subjected to western blot with vaccine immune antibodies and natural infection antibodies, the difference is obvious. The gene expression product BLSJ-3 is taken as an indirect enzyme-linked immune adsorption assay (ELISA) coating antigen to detect hundreds of brucellosis clinical serum samples, and differential diagnosis effect is excellent.