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Reagent box for enzyme linked immunosorbent assay of EB virus protease and its preparation

A diagnostic kit and EB virus technology, applied in the field of EB virus recombinant polypeptide preparation enzyme-linked immunosorbent assay kits, can solve problems such as no prescription, no comparative objective standard, etc.

Inactive Publication Date: 2005-02-23
SINOCLONE LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

How to distinguish between high and low levels, there is no more objective standard
In addition, when using ELISA, how to overcome the differences between each batch number and the difference between each test with the same batch number kit, there is currently no good solution.

Method used

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  • Reagent box for enzyme linked immunosorbent assay of EB virus protease and its preparation
  • Reagent box for enzyme linked immunosorbent assay of EB virus protease and its preparation
  • Reagent box for enzyme linked immunosorbent assay of EB virus protease and its preparation

Examples

Experimental program
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Effect test

Embodiment Construction

[0048] 1. Epstein-Barr virus protein enzyme-linked immunosorbent assay kit, including: positive control serum, control serum, enzyme substrate, stop reaction solution, dilution buffer, washing buffer and EB virus target antigen, in which the EB virus protein is: EBNA1 (BKRF1) protein, Zta (BZLF1) protein and VCA-p18 protein (ie 18Kd VCA or BFRF3 protein).

[0049] The kit also includes a diagnostic reference serum equivalent to the optimized cut-off optical density value. The relative optical density value (rOD) of the intersection point of the sensitivity curve and the specificity curve was the optimal cut-off value for the reference serum.

[0050] The preparation method of the EB virus protein enzyme-linked immunosorbent assay kit, including preparation of recombinant antigen, purification labeling, titer titration, preparation of coated plate, sealing, drying and packaging, preparation of enzyme-labeled antibody concentrate, preparation of diluent, positive control Prepar...

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Abstract

A method for preparing EB viral protein enzyme-linked immunosorbent diagnostic kit includes selectng EBNAl (BKRF1) prote, Zta (BZLF1) protein and VCA-p18 protin in EB viral protein for diagnosing nasopharyngeal carcinoma of serodiagnosis as target antigen for detecting antibody level in blood serum, using glutathione-transferase gene fusion system to carry on clone, presentation and purification of EB viral protein for creating diagnostic kit.

Description

[technical field] [0001] The invention relates to a kit for serological diagnosis of nasopharyngeal carcinoma and other Epstein-Barr virus-related diseases and a preparation method thereof, in particular to a kit for detecting antibody levels in serum by using a specific Epstein-Barr virus protein as a target antigen and preparation of Epstein-Barr virus recombinant polypeptides ELISA kit method. [Background technique] [0002] In my country, almost 100% of people over the age of four have been infected with EB virus, and more than 80% of healthy adults are EB virus carriers. There may be one or 2 to 3 (not multiple, ie not more than 3) low-level (not high-level) anti-EB virus antibodies in the serum of Epstein-Barr virus carriers. Southern my country, especially the Pearl River Delta and Hong Kong, is a high-incidence area of ​​nasopharyngeal carcinoma in the world, and the occurrence of nasopharyngeal carcinoma is closely related to Epstein-Barr virus infection. Southern...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/535G01N33/569
Inventor 吴文翰陈泓霖陈国雄吴子柏
Owner SINOCLONE LTD
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