533 results about "Antibody level" patented technology

A method for determining the presence of food allergy or food intolerance and their cross-reactive tissue antigens is disclosed. The method includes determining a level of antibodies against a dietary antigen in a mucosal sample from the patient and comparing the level with normal levels of the antibodies. Dietary antigens that were tested include milk and milk products; eggs and egg products; meat and meat products; fish, mollusks, and crustaceans and their products; oils, fats, and their products; grains and grain products; pulses, seed, kernels, nuts, and their products; vegetable and vegetable products; fruit and fruit products; sugar, sugar product, chocolate products, and confectionary; and spices.

The invention discloses a method for propagating Chinese medicine ducks, which comprises the following steps: I, selecting a Chinese medicine formulation; II, fermenting and processing the Chinese medicine formulation to obtain zymotic fluid and fermentation body; and III, applying the zymotic fluid and the fermentation body in a method for propagating the Chinese medicine ducks in different stages. Owing to the method for propagating Chinese medicine ducks, the propagation of the Chinese medicine ducks no longer needs hormone and antibiotic, zymotic Chinese medicine formulation elaborates drug action to the maximum extent, and large-scale propagation is realized; the method has the characteristics of simple operation, safety and environment-protection, natural state, zero pollution, drugand food isogeny, pure, zero side effect, absence of no drug resistance and the like, and can effectively reduce drug residual of duck production; the physique of the ducks can be remarkably enhanced, the immunity and antibody level are improved, and generation of diseases is reduced; and the effects of health care and treatment are achieved, the smell of dejection of the ducks is radiated, and the ecological environment is ameliorated.

The invention discloses a pig foot-and-mouth disease virus O-type broad spectrum multi-epitope recombination antigen and application thereof and belongs to the field of biological vaccines. The pig foot-and-mouth disease virus O-type broad spectrum multi-epitope recombination antigen adopts a strategy of an antigenized antibody, after main antigen epitopes of a plurality of strains of pig foot-and-mouth disease virus O-type are connected in series reasonably, the plurality of strains of pig foot-and-mouth disease virus O-type are coupled with a pig intravenous gamma globulin (IgG) heavy chain constant region to construct the pig foot-and-mouth disease virus O-type broad spectrum multi-epitope recombination antigen, and after ration through a Bio-Rad protein ration kit, the pig foot-and-mouth disease virus O-type broad spectrum multi-epitope recombination antigen and recombination foot-and-mouth disease virus 3D protein are matched to prepare the vaccines. Animal immunity testing results show that the vaccines can stimulate an organism to generate high-titer protective antibodies when the vaccines are used independently or matched with the recombination foot-and-mouth disease virus 3D protein to be used, an antibody level is higher than a national standard, and good application prospects are achieved.

The invention discloses a raising method for traditional Chinese medicine free-range chickens, which includes the following steps: (1) site selection and chicken house construction; (2) disinfection; (3) raising management: purebred, robust chicks are chosen; the temperature of the chick-raising houses is controlled at 32 DEG C to 33 DEG C for the age of 0 to 1 week, and is decreased by 1 DEG C to 2 DEG C each week, and at the age of 4 weeks, heating is stopped; the chicks drink water and are fed; drinking water vaccination is adopted at the previous stage, and an injection method is adopted for vaccination at the later stage; after being raised for 1 month, the chicks enter the later stage of raising, later-stage feed is granular feed, and the chicks are fed after 10 a.m. each day, fed once after 3 p.m. and fed once again before sleep; in the whole raising period, water is not stopped. The growing speed of the free-range chickens is high, constitution is strong, the immunity and antibody level of the free-range chickens are increased, the incidence rate of the free-range chickens is decreased, the raising method for traditional Chinese medicine free-range chickens is easy to operate, is safe, environment-friendly and pollution-free, and the traditional Chinese medicine free-range chickens can be raised on a large scale.

The invention provides an immunity enhancing agent, an inactivated vaccine, and a preparation method thereof. The invention relates to the field of biopharmaceutical. The immunity enhancing agent comprises 0.1-21mg/mL of monophosphoryl lipid A, 1.5-125mg/mL of muramyl dipeptide, and 0.7-4.5mg/mL of beta-glucan. The invention also provides the inactivated vaccine comprising the immunity enhancing agent, and a preparation method of the inactivated vaccine. According to the invention, the immunity enhancing agent is mixed with an inactivated antigen solution, such that a water-phase solution is obtained; and the water-phase solution is mixed with an oil-phase solution, such that the inactivated vaccine is obtained. According to the immunity enhancing agent provided by the invention, with a synergetic effect of the components, body immunity level can be improved, and immune response to antigen can be improved, such that antibody level after immunization can be increased, immune window period can be shortened, and vaccine immunization effect can be enhanced. According to the inactivated vaccine comprising the immunity enhancing agent, antibody level after immunization is high, a protection period is long, and immunization window period is short.

The method for quantitative determination of the level of natural autoantibodies in human biological fluids, when as a solid phase of physical sorption is used the solid phase of physical sorption, coated with streptavidin, and the solid phase of physical sorption is treated with preliminary biotinylated antigen and blocking agent for closing the sites of nonspecific binding at the solid phase of physical sorption, for which purpose are used proteins, biotinylated according to standard procedure. As the conjugate-containing solution are used enzyme-labeled monoclonal and polyclonal antibodies, which react with one or all isotypes of human immunoglobulins. In addition, the tested biological fluid is preliminary diluted in a buffer, containing proteins which are used for closing the sites of nonspecific binding at solid phase of physical sorption, and also substances protecting natural autoantibodies from destruction during heat treatment, and subjected to heat treatment. For each tested specimen of biological fluid, a control solid phase of physical sorption is used, and the number of natural autoantibodies is determined with the use of a calibration curve which is plotted using monoclonal or polyclonal antibodies to antigen.

A method for preparing EB viral protein enzyme-linked immunosorbent diagnostic kit includes selectng EBNAl (BKRF1) prote, Zta (BZLF1) protein and VCA-p18 protin in EB viral protein for diagnosing nasopharyngeal carcinoma of serodiagnosis as target antigen for detecting antibody level in blood serum, using glutathione-transferase gene fusion system to carry on clone, presentation and purification of EB viral protein for creating diagnostic kit.

The invention, which belongs to the technical field of vaccines, particularly relates to an African swine fever neutralizing epitope subunit vaccine that is mainly composed of African swine fever neutralizing epitope fusion protein. The African swine fever neutralizing epitope fusion protein mainly comprises a B cell neutralizing epitope peptide fragment including at least one neutralizing epitopepeptide of p72 protein, at least one neutralizing epitope peptide of p54 protein and at least one neutralizing epitope peptide of p30 protein. With the African swine fever neutralizing epitope fusionprotein, the risk of accelerating virus infection possibly caused by non-neutralizing antibodies can be effectively eliminated and the immune safety of the fusion protein can be effectively improved.Besides, when the African swine fever neutralizing epitope fusion protein is used for immunizing pigs, the antibody level obviously higher than that of a control group can be generated after one immunization.

The invention provides a novel coronavirus detection test strip as well as a preparation method and application thereof. The test strip comprises a binding pad and an analysis membrane, and the binding pad is coated with a luminescent substance labeled 2019-nCoV recombinant antigen and a mouse anti-human HCG monoclonal antibody; a T2 detection line, a T1 detection line and a quality control line are sequentially arranged on the analysis membrane along the chromatography direction; the T2 detection line is coated with a mouse anti-human IgG monoclonal antibody, the T1 detection line is coated with a mouse anti-human IgA monoclonal antibody and a mouse anti-human IgM monoclonal antibody, and the quality control line is coated with a goat anti-mouse IgG polyclonal antibody. The test paper card can simultaneously determine the positive conditions of IgA antibody, IgM antibody and IgG antibody in serum of a patient, can more accurately detect the early antibody level condition in the body of the patient, assists in judging different periods of novel coronavirus infection of the patient, and improves the sensitivity and specificity of novel coronavirus detection.

The invention relates to a vitamin water aqua. The raw materials of the water aqua comprise liposome, vitamin A, vitamin D, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin H, folic acid and solubilizer. In the invention, liposoluble vitamins are coated in a microcapsule structure of the liposome and form a homogenized and stable water aqua with water-soluble vitamins. The water aqua has stable storage character in a long time, smaller grain diameter of components and a cytoid structure and can activate the self immunologic function after entering an animal body to keep the immune level of organisms in a higher state; and meanwhile, a plurality of vitamins can be effectively stored in the organism, so that the invention avoids the problems of the loss and the insufficient utilization rate of the vitamins in the metabolic process, remarkably improves the utilization rate and the conversion rate of feed, improves the antibody level of animal organisms and enhances the disease resistant capability.

The invention relates to a feed additive comprising SOD. The feed is characterized in comprising the following components, by weight: 0.8 parts of SOD, 0.6-0.8 parts of probiotics, 1-2 parts of multivitamins, and 7-8 parts of serum globulin powder. The activity of SOD is no lower than 6000u/mg, and protein is no lower than 4.0%. Compared to prior art, the feed additive provided by the invention has the advantages that: (1) the feed additive comprises no hormone or antibiotic, and is safe, green, healthy, and pollution-free; (2) with the additive, free radicals in pig bodies can be removed, pig immunity and disease resistance can be improved, pig resistance can be improved, and pig daily weight gain can be increased; (3) with the additive, pig antibody level can be increased, such that pig immunity can be improved, pig disease resistance can be improved, and pig survival rate can be improved; and (4) the additive provides necessary copper, manganese, multivitamins, proteins and immune growth promotion factors for pigs during a growth process, such that pig reproductive capacity and growth rate can be improved.

The invention relates to an indirect ELISA (enzyme-linked immunosorbent assay) method for detecting a porcine reproductive and respiratory syndrome antibody, which comprises the following steps: by using a pGEX-6p-1 prokaryotic expression vector, performing tandem repeat on two epitopes to improve the antigen activity of an expressed protein, thus constructing a gene engineering bacterium BLpGEX-6p-GP5 capable of realizing secretory expression of the GP5 protein dominant antigen epitopes, wherein one epitope is a linear conservative neutralizing epitope (epitope B) of a screened PRRSV (porcine reproductive and respiratory syndrome virus) GP5 protein, which can be identified by a monoclonal antibody and can also be identified by porcine anti-PRRSV neutralizing serum, and the other epitope is a high-variability immunodominant epitope (A); and purifying and renaturing the expressed recombinant protein, and coating an ELISA plate, thus establishing the indirect ELISA method for detecting a PRRSV antibody to detect the PRRSV antibody level in porcine serum. Results show that the method has the characteristics of favorable repetitiveness and high specificity and can be used for PRRSV serological search.

The invention discloses an inactivated vaccine for preventing and treating novel goose astroviruses and a preparation method thereof. The inactivated vaccine is prepared from the effective dose of inactivated goose astrovirus strain virus liquid in prevention or treatment. A preservation number of the goose astrovirus strain is CCTCC NO: V201808. The prepared novel goose astrovirus inactivated vaccine is good in safety, any local or general adverse effects caused by the vaccine do not appear, and the detection of each index is stable and effective. After the inactivated vaccine is used for immunizing a young goose, the young goose can acquire the higher antibody level, and a persistent period is long. The infection and outbreak of the novel goose astroviruses can be prevented and treated specifically, and the effective immune protection is provided for a goose group. In addition, a production technology for the vaccine is simplified, and the large-scale production can be realized.

The invention belongs to the field of feed and particularly relates to feed specially used for culturing Nanyang yellow cattle. The feed comprises corn flour, distillers grains of baijiu, oat powder, soybean meal, peanut meal, tenebrio molitor, cockroach powder, rapeseed cake, calcium carbonate, amino acids, folic acid, choline chloride, an antiseptic, enzyme preparations, plant extracts, probiotics, sodium alginate, citric acid and sodium carboxymethylcellulose. A plurality of the composite probiotics, the enzyme preparations and the plant extracts are added into the feed, thus providing a plurality of beneficial compounds for yellow cattle, maintaining intestinal tract ecological balance of the yellow cattle, improving trace element absorption, stimulating growth and development of intestinal tract immune organs, increasing the animal antibody level and macrophage activity, boosting humoral immunity and cell-mediated immunity of a body, timely killing invaded pathogenic bacteria, boosting resistance of animals to a plurality of diseases, and largely reducing the morbidity and the mortality.

The invention aims at providing an H9 subtype avian influenza virus strain. Vaccine is prepared by the virus strain to solve the problem that existing H9 subtype avian influenza virus vaccine is poor in immune effect caused by a novel virus. The H9 subtype avian influenza virus QDY strain (Avian Influenza Virus) is preserved in the typical culture preservation center in China in Wuhan University on April 29, 2015, and the preservation number is CCTCC NO: V201517. After the H9 subtype avian influenza virus (QDY strain) is inoculated to a chick embryo, a virus solution is collected, after ultrafiltration concentration and formaldehyde solution inactivation are performed, oil adjuvant is added, and then mixing and emulsifying are performed to prepare the vaccine. According to the prepared vaccine, the antibody level after immunization can be improved, the antibody uniformity after immunization can be improved, and the immune effect of the vaccine can be guaranteed; the vaccine has the advantages of being efficient and good in safety.

The invention provides a heat-resistant freeze-drying protective agent applied to animal freeze-dried live vaccine. The heat-resistant freeze-drying protective agent comprises 1%-3% of glutin, 1%-8% of lactose and the like, wherein, all substances are dry components, and distilled water takes all the rest percentage. The invention also provides a method for preparing the protective agent. ND live vaccine prepared by the protective agent of the invention can be conserved for more than 24 months at the temperature of 2 DEG C to 8 DEG C. Immunity test shows that the average antibody level of immunized chicken is 1 to 2 titers higher than the average antibody level of tested chicken immunized by the ND live vaccine which is freeze-dried by ordinary protective agent, and the antibody level is uniform. Therefore, the freeze-drying protective agent can be widely applied to the production and conservation of various animal live vaccines and has wide application prospect.

The invention discloses a ginsenoside-containing vegetable oil adjuvant and a preparation method and application thereof. The ginsenoside-containing vegetable oil adjuvant is characterized in that edible vegetable oil is used as the adjuvant oil; the ginsenoside-containing vegetable oil adjuvant comprises 10 to 100mu g/mL ginsenoside. According to the preparation method, the ginsenoside-containing vegetable oil is utilized for preparing vaccines, so that the vaccine safety can be greatly improved, and the potential hazard of the general mineral oil adjuvant on food hygiene is avoided; after being mixed, ginsenoside and vegetable oil play the effect of collaborating with the adjuvant, thus the valence of antibody is raised by 4.7 times, and the antibody level of the vaccines is also greatly improved; in addition, the prepared vaccine is high in mobility, convenient for inoculation, small in local stimulation, and thus relieving pain on animals; when the ginsenoside-containing vegetable oil adjuvant is applied to vaccine production, the existing production process is remained, and the method is simple and convenient.

The invention relates to a yolk antibody injection against canicola fever and canine parvovirus and a preparation method thereof. The yolk antibody injection against canicola fever and canine parvovirus is extracted and prepared by the collected yolk antibody of the immunized laying hens which are immunized by the bivalent vaccine of the canicola fever and canine parvovirus. The yolk antibody injection against canicola fever and canine parvovirus prevents the diseases by improving the antibody level of canine bodies to the canicola fever and the canine parvovirus. The yolk antibody injection against canicola fever and canine parvovirus and a preparation method thereof have the beneficial effect that the anti-canicola fever and canine parvovirus disease egg yolk antibody injection is provided with faster effect in the onset of canicola fever and canine parvovirus diseases. The yolk antibody injection against canicola fever and canine parvovirus and the preparation method thereof has the advantages of obvious efficacy, low cost, fast effect, no side effects, no generated drug resistance, simple preparation, wide raw material resource, convenient use and easy application.

The invention provides a preparation method of a bivalent vaccine by taking newcastle disease virus La Sota strain and infectious bronchitis virus N-S multi-epitope protein as antigen. To immunize 21-day-old chickens, the bivalent vaccine prepared by the method disclosed by the invention can be used for simultaneously preventing newcastle disease and nephrotropic infectious bronchitis virus infection, and the antibody level of the bivalent vaccine is higher than that of respectively immunizing by single vaccine, so that immunological stress is effectively relieved and immunity time is reduced.

The invention relates to a colloidal gold test paper strip for carrying out the quick detection of the O-shaped foot and mouth disease antibody level of artiodactyls, the preparing method of the test paper strip and a specific detection method. A sample application absorption cushion made of porous fiber material, an immune gold label layer which is arranged on the tail end of the sample application absorption cushion and is connected with the sample application absorption cushion, a detection section made of nitrocellulose film, and an absorption section which is made of water absorption material and is arranged on the tail end of the detection section and is connected with the detection section are fixed on the base plate of the test paper strip. A quality control line is an O-shaped foot and mouth disease resistant rabbit or guineapig IgG antibody, a detection line is coated with O-shaped foot and mouth disease virus, and the O-shaped foot and mouth disease virus of the detection line of the detection section and the colloidal gold label attached to the immune gold label layer is natural virus, or DNA recombination expression foot and mouth disease virus, or the decomposed antigen of the foot and mouth disease.