The invention relates to a multi-respiratory tract antigen detection card, which includes influenza A virus antigen test strips, influenza B virus antigen test strips, respiratory adenovirus antigen test strips, respiratory syncytial virus antigen test strips and Mycoplasma pneumoniae antigen test strips, Influenza A virus antigen test strips, Influenza B virus antigen test strips, Respiratory adenovirus antigen test strips, Respiratory syncytial virus antigen test strips and Mycoplasma pneumoniae antigen test strips all include Colloidal gold conjugate pads, colloidal gold conjugate pads include streptavidin conjugate pads and double-nanoparticle double-labeled antibody conjugate pads; the test strips in this test card can fully react biological raw materials and improve the sensitivity of antigen detection , effectively reducing missed detection, and adding blocking agents to the sample pad to improve specificity, and can simultaneously detect influenza A virus, influenza B virus, respiratory adenovirus, respiratory syncytial virus, and Mycoplasma pneumoniae antigens.