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42 results about "Pathogenic avian influenza" patented technology

Avian influenza—known informally as avian flu or bird flu is a variety of influenza caused by viruses adapted to birds. The type with the greatest risk is highly pathogenic avian influenza (HPAI).

Nucleic acid detection kit for synchronously identifying and diagnosing newcastle disease virus and avian influenza virus

The invention belongs to the field of inspection and quarantine technology. Specifically, the invention is a nucleic acid detection reagent kit for synchronous discriminating and diagnosing avian influenza virus and newcastle disease virus. The detection reagents of the reagent kit include extraction reagent for extracting virus by silicon gel absorption column method, detection amplification reagent for detecting nucleic acid by RT-PCR Taq Man fluorescent probe method, and pretreatment liquid for solid tissue specimen for extracting virus RNA. Further, the invention employs in vitro transcription RNA as a positive contrast of the reagent kit. The reagent kit can rapidly and synchronously discriminate and diagnose avian influenza virus and newcastle disease virus which are highly infectious among avian plagues and have similar symptom, determine current major prevalent subtypes, such as H5, H7, H9, etc., and discriminate whether a infection source is an avian influenza having high pathogenicity, non-pathogenic avian influenza or mildly pathogenic avian influenza to human. The reagent kit is suitable for livestock and veterinarian station, import and export inspection and quarantine bureau, as well as other laboratories, and can be used for large-scale detection of influenza and epidemic surveillance.
Owner:SHANGHAI KEHUA BIO ENG

Recombinant H7N9 subtype avian influenza virus-like particle, and preparation method and application thereof

The invention discloses a recombinant H7N9 subtype avian influenza virus-like particle, and a preparation method and application thereof. Recombinant baculoviruses for expressing HA protein, NA protein and M1 protein of an H7N9 highly pathogenic avian influenza virus are respectively constructed on the basis of a recombinant baculovirus insect cell culture system. The three recombinant baculovirusstrains are used for co-infecting suspended insect cells, so that the H7N9 subtype avian influenza virus-like particle capable of being self-assembled in the cells can be obtained. After the concentration and purification with cane sugar with different gradient concentrations through an ultrafiltration tube, the H7N9 subtype avian influenza virus-like particle is mixed and emulsified with an adjuvant to prepare a vaccine. When a chicken is immunized by the prepared vaccine, the body can be induced to generate a specific antibody; and the advantages of high immunogenicity, good safety, high hereditary stability and the like are realized. After the attack by a lethal dose of H7N9 subtype highly pathogenic avian influenza viruses, the complete clinical protection can be provided, and virus expelling of the chicken is obviously inhibited. The invention provides a novel method for preventing H7N9 subtype avian influenza virus infection, and also lays a foundation for the development of a novel influenza virus vaccine.
Owner:YANGZHOU UNIV

Recognition method of highly pathogenic avian influenza virus hemagglutinin protein

The invention discloses a highly-pathogenic avian influenza virus hemagglutinin protein identification method, which can be used for highly-pathogenic avian influenza virus hemagglutinin protein identification and provide a reference for understanding the structural characteristics of the highly-pathogenic avian influenza virus hemagglutinin protein; through the identification of the hemagglutinin protein, the method can indirectly distinguish the degree of the pathogenicity of the avian influenza virus strain to which the hemagglutinin protein belongs. The method comprises the following steps: a), to establish an amino acid holographic topological structure score vector, based on principal component analysis; b), to apply the amino acid holographic topological structure score vector to characterize the structure of the influenza virus hemagglutinin protein; c), to carry out normalized processing to the characteristic variable of each influenza virus hemagglutinin protein through a self-cross-covariance method; d), to establish an avian influenza virus hemagglutinin protein identification model through an RBF kernel-based support vector machine; e), to establish a highly-pathogenic avian influenza virus hemagglutinin protein identification model through the RBF kernel-based support vector machine.
Owner:CHONGQING UNIV

Method for detecting H5-subtype and H7N9-subtype highly pathogenic avian influenza viruses as well as H9-subtype avian influenza virus

The invention provides a rapid detection method capable of synchronously detecting and differentiating H5-subtype and H7N9-subtype highly pathogenic avian influenza viruses as well as H9-subtype avianinfluenza virus. By analyzing nucleotide sequences of HA genes of an H5-subtype highly pathogenic avian influenza virus, an H7N9-subtype highly pathogenic avian influenza virus and an H9 subtype avian influenza virus isolated in recent years, as well as designing primers and probes in conservative regions, a rapid real-time fluorescence quantitative RT-PCR nucleic acid detection method for the H5-subtype and H7N9-subtype highly pathogenic avian influenza viruses as well as the H9-subtype avian influenza virus is established. The rapid real-time fluorescence quantitative RT-PCR nucleic acid detection method for the H5-subtype and H7N9-subtype highly pathogenic avian influenza viruses as well as the H9-subtype avian influenza virus provided by the invention has the advantages of being highin sensitivity, short in detection time, and free of open links such as electrophoresis and the like; moreover, only one fluorescent PCR instrument is required for accomplishing detection on the threesubtypes of the avian influenza viruses in a closed reaction tube. In addition, quick judgment on results is allowed for real-time viewing of response curve is feasible during the detection process.
Owner:CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENT

Method and device for identifying pathogenic influence of concurrent bacteria infection against poultry influenza virus infection

A method for determining the pathogenic affection of concurrent bacterial infection to avian influenza virus includes such steps as inoculating the bacterium able to secrete subtilisinoid and / or the bacterium able to secrete tryptase and the low-pathogenicity avian influenza virus in culture medium of CEF in a particular mode, culturing, and observing if CPE presents. Its cell culture equipment is composed of cell culture bottle and bacteria culture chamber. Its advantages are simple method, short time, high correctness and low cost.
Owner:SUN YAT SEN UNIV

Liquid-phase chip for detecting flu and H5N1 avian influenza virus and specific detection primer and preparation method thereof

The invention relates to a method for detecting influenza viruses, in particular to a method for detecting influenza viruses and H5N1 subtype avian influenza virus by a liquid-phase chip, belonging to the technical field of medical monitoring. The method is a fast and highly efficient one which can identify FluA and FluB influenza viruses including the H5N1 subtype avian influenza virus by types.With the utilization of a liquid-phase chip technology and the application of bioinformatics knowledge and related bioinformatics software, the liquid-phase chip which can specifically distinguish H5and N1 gene fragments, NP gene fragment of the FluA influenza virus and HA gene fragment of the FluB influenza virus is manufactured. The method has the advantages that fast detecting and early-stagediagnosis of the FluA and the FluB influenza viruses including the H5N1 subtype highly pathogenic avian influenza virus can be carried out; and a basis for developing other fast and highly effective detecting methods for detecting various subtype influenza viruses is provided. The method is characterized by detecting multiple types of viruses, short response time and high sensitivity, thus determining that the method has wide application prospect.
Owner:JIANGSU PROVINCIAL CENT FOR DISEASE PREVENTION & CONTROL

A kind of recombinant h7n9 subtype avian influenza virus-like particle and its preparation method and application

The invention discloses a recombinant H7N9 subtype avian influenza virus-like particle and its preparation method and application. Based on the recombinant baculovirus insect cell culture system, the expression of H7N9 highly pathogenic avian influenza virus HA protein and NA protein is respectively constructed. , M1 protein recombinant baculovirus. Three strains of recombinant baculoviruses were co-infected in suspended insect cells, and H7N9 subtype avian influenza virus-like particles self-assembled in the cells could be harvested. After being concentrated and purified by ultrafiltration tubes and different gradient concentrations of sucrose, it is mixed with adjuvant and emulsified to prepare the vaccine. The prepared vaccine immunized chickens can induce the body to produce specific antibodies, and has the advantages of strong immunogenicity, good safety, high genetic stability and the like. After challenge with a lethal dose of highly pathogenic avian influenza virus of H7N9 subtype, it can provide complete clinical protection and significantly inhibit the shedding of chickens. The invention provides a new method for preventing H7N9 subtype avian influenza virus infection, and also lays a foundation for the development of new influenza virus vaccines.
Owner:YANGZHOU UNIV

Recognition method of highly pathogenic avian influenza virus hemagglutinin protein

The invention discloses a highly-pathogenic avian influenza virus hemagglutinin protein identification method, which can be used for highly-pathogenic avian influenza virus hemagglutinin protein identification and provide a reference for understanding the structural characteristics of the highly-pathogenic avian influenza virus hemagglutinin protein; through the identification of the hemagglutininprotein, the method can indirectly distinguish the degree of the pathogenicity of the avian influenza virus strain to which the hemagglutinin protein belongs. The method comprises the following steps: a), to establish an amino acid holographic topological structure score vector, based on principal component analysis; b), to apply the amino acid holographic topological structure score vector to characterize the structure of the influenza virus hemagglutinin protein; c), to carry out normalized processing to the characteristic variable of each influenza virus hemagglutinin protein through a self-cross-covariance method; d), to establish an avian influenza virus hemagglutinin protein identification model through an RBF kernel-based support vector machine; e), to establish a highly-pathogenicavian influenza virus hemagglutinin protein identification model through the RBF kernel-based support vector machine.
Owner:CHONGQING UNIV

Avian influenza virus-like particle antigen, vaccine, preparation method of avian influenza virus-like particle antigen and application of avian influenza virus-like particle antigen or vaccine

The invention discloses an avian influenza virus-like particle antigen, a vaccine, a preparation method of the avian influenza virus-like particle antigen and application of the avian influenza virus-like particle antigen or the vaccine. A strain of recombinant baculovirus based on tandem expression of HA, NA and M1 genes of an H7N9 subtype avian influenza virus transfects insect cells and performs self-assembly to form the avian influenza virus-like particle antigen; and the virus-like particle antigen prepared according to the invention is used for preparing the vaccine. After a chicken group is immunized by the vaccine prepared according to the invention, HI antibodies, neutralizing antibodies and IgY antibodies with the higher titer can be generated, 100-percent clinical protection onthe H7N9 subtype highly pathogenic avian influenza virus can be provided, and virus expelling can be effectively inhibited. The rely on the chicken embryos is avoided; the production cost is low; theperiod is short; and higher protection capability can be induced only by once low-dose immunization. A foundation is laid for industrial production of VLP vaccines.
Owner:YANGZHOU UNIV
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