The invention provides an anti-electromagnetic interference substrate and a preparation method thereof, and electronic equipment. The preparation method of the anti-electromagnetic interference substrate comprises the following steps that a base material is provided, and a shielding area needing to be provided with electromagnetic shielding on the base material is determined; metal particles are sprayed on the shielding area of the base material, and thus a metal coating is formed on the shielding area; and the metal particles are one of copper particles, silver particles, gold particles, copper alloy, silver alloy or gold alloy. The metal coating on the shielding area of the base material by spraying one of copper particles, silver particles, gold particles, copper alloy, silver alloy orgold alloy, so that the shielding area can provide good electromagnetic shielding protection; moreover, the quality of the metal coating can be ensured, automatic mass production can be conducted conveniently, manual sticking is not needed, abnormal fitting is avoided, and waste is reduced; and the method is particularly applicable to a substrate with a complicated and sharp surface, and the adaptability is good.