Disposable biological sensor for detecting blood alcohol concentration

A biosensor and alcohol concentration technology, applied in the field of disposable biosensors, can solve the problems of short storage time and harsh working conditions

Inactive Publication Date: 2007-06-27
CHONGQING MEDICAL UNIVERSITY
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Immobilized enzyme electrode method does not require sample pretreatment, but its working conditions are harsh, storage time is short, and problems such as repeatability, reliability, consistency, and mass production process need to be resolved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Disposable biological sensor for detecting blood alcohol concentration
  • Disposable biological sensor for detecting blood alcohol concentration
  • Disposable biological sensor for detecting blood alcohol concentration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0062] To prepare a disposable biosensor for detecting blood alcohol concentration, follow the steps below:

[0063] (1), the preparation of silver-plated sheet electrode chooses smooth, flat plastic film, cuts into width and is 10.5mm, and length is 60mm long strip, and plastic film sheet is placed in 10%NaOH solution, heats 3 minutes, takes out, Wash with deionized water, dry for later use; cover a layer of protective film on the front of the spare film, reserve the shape and size of the electrode and its lead wires, and completely cover the back with the protective film. Place the film at a volume ratio of 1:1 2% glucose and 2% silver ammonia solution, mix well, let stand at room temperature for 20 minutes, until a dense silver mirror is formed on the film, take out the film, wash with deionized water and dry, tear off Protective film, that is, the required silver-plated sheet electrode;

[0064] (2) The basic electrode is printed using screen printing technology in the fo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
widthaaaaaaaaaa
Login to view more

Abstract

Through one-off screen-printing, working electrode is prepared by printing commercial carbon ink on plastic film. Printing Ag/AgCl ink produces a counter electrode and a reference electrode. Then, analysis fluid of alcohol dehydrogenase is dropped to a reaction cavity between the working electrode and the reference electrode. After natural seasoning electrode piece, using double face adhesive tape sticks the transparent glue onto the electrode piece so as to form a small electrolytic cell between the transparent glue and the plastic film. When enzyme electrode immerges to blood sample to be tested, reaction is taken place between the enzyme electrode and the sample. Under action of electric field, electron transfer occurs to for current. Based on coincidence relation between electric chemical signal and concentration of substrate to be measured, alcohol concentration in blood can be measured. Features are: high sensitivity, accuracy, low cost, one-off use, and batch production.

Description

technical field [0001] The invention relates to a biosensor, in particular to a disposable biosensor for detecting blood alcohol concentration. Background technique [0002] The occurrence of alcoholic liver disease is related to ethanol (alcohol) consumption, that is, the average daily alcohol consumption and excessive drinking. Studies have shown that drinking 50 grams of alcohol per day for more than 5 years can cause liver damage. The amount and duration of drinking are directly related to the occurrence of alcoholic fatty liver, but have little to do with the type of alcohol. If the amount of alcohol consumed is greater than 50-120 grams / day, the incidence rate will increase by 5-25 times; if drinking 300 grams / day of pure alcohol, fatty liver will appear after 7 days. The pathogenesis of alcoholic fatty liver is that 90% of the alcohol entering the body is metabolized in the liver, which can affect all aspects of fat metabolism and eventually lead to fat accumulation...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/98G01N27/413
Inventor 谢国明邓世雄李琼罗鹏
Owner CHONGQING MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap