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Polypeptide vaccine for treating diseased induced by high pathogenic avian influenza virus H5N1

A bird flu virus, H5N1 technology, applied in the field of biomedicine, can solve the problem of reduced replication efficiency in mice, and achieve the effect of facilitating automatic mass production, obvious effect, and convenient transportation and storage

Inactive Publication Date: 2009-03-11
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Watanabe et al. found that after cutting off 11 amino acids at the hydroxyl end of H5N1 virus M2, the virus had similar growth ability compared with the wild type, but the replication efficiency in mice was greatly reduced

Method used

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  • Polypeptide vaccine for treating diseased induced by high pathogenic avian influenza virus H5N1
  • Polypeptide vaccine for treating diseased induced by high pathogenic avian influenza virus H5N1
  • Polypeptide vaccine for treating diseased induced by high pathogenic avian influenza virus H5N1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: its amino acid sequence can be:

[0027]

[0028] The polypeptide can stimulate HLA-A*0201 positive human peripheral blood mononuclear cells in vitro, increase the number of these peripheral blood mononuclear cells transformed into cytotoxic T lymphocytes (CTL for short) by 60%, and activate CTL to dissolve For cells with the H5 gene of the highly pathogenic avian influenza virus H5N1, the lysis rate is 83.10%.

Embodiment 2

[0029] Embodiment 2: its amino acid sequence can also be:

[0030]

[0031] The polypeptide can stimulate peripheral blood mononuclear cells of HLA-A*0201 positive human body in vitro, increase the number of these peripheral blood mononuclear cells transformed into cytotoxic T lymphocytes (CTL for short) by 50%, and activate CTL to dissolve For cells with the H5 gene of the highly pathogenic avian influenza virus H5N1, the lysis rate is 79.0%.

Embodiment 3

[0032] Embodiment 3: its amino acid sequence can also be:

[0033]

[0034] The polypeptide can stimulate HLA-A*0201 positive human peripheral blood mononuclear cells in vitro, increase the number of these peripheral blood mononuclear cells transformed into cytotoxic T lymphocytes (CTL for short) by 55%, and activate CTL to dissolve For cells with the H5 gene of the highly pathogenic avian influenza virus H5N1, the lysis rate is 68.0%.

[0035] The amino acid sequence method described above is an existing mature technology, which is made according to the following method:

[0036] Adopt the standard Fmoc scheme, initially select 0.0125mmol, PSC resin (produced by ABI company, batch number A5F013), respectively according to the sequence characteristics described in claim 1, 2, 3 or 4, make the peptide chain extend from the C terminal to the N terminal one by one , the consumption of each amino acid raw material (produced by American ABI Company) is 0.1 mmol. Various amino ...

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Abstract

The invention relates to a polypeptide vaccine for treating diseases caused by highly pathogenic avian influenza virus H5N1, which is characterize in that: the amino acid sequence is as follows: amino terminal-QIGNISIWV-carboxyl terminal; and lysine is adopted as a linker to connect a plurality of amino acid sequences to form a multi-copy series structure. The invention has the advantages of convenient transportation and preservation and automated mass production, and can activate specific cytotoxic T lymphocyte (CTL) which can effectively kill and lyses target cells with the N1 protein of the highly pathogenic avian influenza virus H5N1 without toxicity and side effects.

Description

(1) Technical field [0001] The invention relates to the field of biomedicine, in particular to a polypeptide drug or vaccine for diseases caused by highly pathogenic avian influenza virus. (two), background technology [0002] Avian influenza (Avian influenza) is abbreviated as bird flu, which is a widespread infectious disease in poultry caused by type A influenza virus (AIV). According to incomplete statistics, the global bird flu epidemic has spread to 47 countries and regions, and there have been more than 195 cases of human bird flu, of which at least 110 people died, with a mortality rate as high as 56.4%. The avian flu that occurred in Hong Kong in 1997 caused human morbidity and death, which aroused great attention from all over the world. AIV can be divided into 16 H subtypes (H1-H16) and 10 N subtypes (N1-N10) according to the differences in the antigenicity of hemagglutinin (HA) and neuraminidase (NA). [Alexander DJ. A review of avian influenza in different bird...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K39/145A61P31/16
Inventor 吕凤林田菲菲梅虎张一李元朝
Owner CHONGQING UNIV
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