Human epididymis protease profilin immunological contraception polypeptide

A protease inhibition, immune contraceptive technology, applied in the field of biomedicine

Inactive Publication Date: 2010-02-03
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the immune response triggered by many natural antigens cannot meet the needs of preventing infection or preventing disease, in order to improve the protection of protein

Method used

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  • Human epididymis protease profilin immunological contraception polypeptide
  • Human epididymis protease profilin immunological contraception polypeptide
  • Human epididymis protease profilin immunological contraception polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: The basic amino acid sequence of the present invention is: amino-terminal-LSEIKGVIVHRLEGVGGGMFVYGGCQGNNNN-carboxyl-terminal; using lysine as a linker, multiple basic amino acid sequences form a multi-copy tandem structure.

[0056] The use of the basic amino acid sequence referred to in the present invention:

[0057] Mix with Freund's adjuvant in equal amount at a dose of 80 micrograms / mouse / time, and inject into the subcutaneous tissue of the groin of Balb / c mice; 7 days later, inject Balb / c at a dose of 50 micrograms / mouse / time in the same way The subcutaneous tissue site of the groin of the mouse; after another 14 days, inject the subcutaneous tissue site of the groin of the Balb / c mouse at a dose of 50 μg / time / only in the same way. After 28 days of observation, the tail veins of the immunized mice were bled, and their serum was collected. The antibody titer in the mouse serum was detected by enzyme-linked immunosorbent assay (ELISA), which was significan...

Embodiment 2

[0090] Embodiment 2: The basic amino acid sequence of the present invention can also be:

[0091]

[0092] Main reagents and their preparation:

[0093] Coating diluent (0.05mol / L sodium carbonate-sodium bicarbonate buffer, pU9.6) composition: Na 2 CO 3 1.5g, NaHCO 3 2.9g, Na 2 N 3 0.2g, add double distilled water to 1000ml, adjust to pH9.6.

[0094] Blocking solution (5% calf serum / PBS solution) composition:

[0095] Calf serum 50ml, add PBS (pH7.4) 950ml.

[0096] Phosphate buffered saline (PBS):

[0097] A liquid (0.2mol / L sodium dihydrogen phosphate aqueous solution) composition: NaH 2 PO 4 ·H 2 27.6g of O was dissolved in 1000ml of ultrapure water.

[0098] B solution (0.2mol / L disodium hydrogen phosphate aqueous solution) composition: Na 2 HPO 4 ·7H 2 O 53.6g (or Na 2 HPO 4 12H 2 O71.6g or Na 2 HPO 4 2H 2 O 35.6g) was dissolved in 1000ml of ultrapure water.

[0099] Sample diluent (PBS, 0.01mol / L phosphate-buffered saline) composition: PBA solu...

Embodiment 3

[0122] Embodiment 3: The basic amino acid sequence of the present invention can also be:

[0123]

[0124] Main reagents and their preparation:

[0125] Coating diluent (0.05mol / L sodium carbonate-sodium bicarbonate buffer, pU9.6) composition: Na 2 CO 3 1.5g, NaHCO 3 2.9g, Na 2 N 3 0.2g, add double distilled water to 1000ml, adjust to pH9.6.

[0126] Blocking solution (5% calf serum / PBS solution) composition:

[0127] Calf serum 50ml, add PBS (pH7.4) 950ml.

[0128] Phosphate buffered saline (PB):

[0129] A liquid (0.2mol / L sodium dihydrogen phosphate aqueous solution) composition: NaH 2 PO 4 ·H 2 27.6g of O was dissolved in 1000ml of ultrapure water.

[0130] B solution (0.2mol / L disodium hydrogen phosphate aqueous solution) composition: Na 2 HPO 4 ·7H 2 O 53.6g (or Na 2 HPO 4 12H 2 O71.6g or Na 2 HPO 4 2H 2 O 35.6g) was dissolved in 1000ml of ultrapure water.

[0131] Sample diluent (PBS, 0.01mol / L phosphate-buffered saline) components: PBA soluti...

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Abstract

A human epididymis protease profilin immunological contraception polypeptide is characterized in that the basic amino acid sequence of the polypeptide is amino terminal-LSEIKGVIVHRLEGVGGGMFVYGGCQGNNNN-carboxyl terminal; lysine can be used as linker to combine a plurality of the basic amino acid sequences together to compose multiple copies of series connection structure. The invention has the advantages that the product is easy to transport and preserve and the automated production of the product can be realized; the invention has effective immunogenicity and antigenicity, obvious immune effect, high effective and reversible contraceptive effect, safe use and no side effect.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a contraceptive polypeptide vaccine. Background technique [0002] Family planning is the basic national policy of our country. In order to effectively control excessive population growth, developing new safe and reversible contraceptive measures has always been an important topic of family planning research. Male contraception is currently limited to a choice between a condom and a vasectomy. The former has a high failure rate, while the latter is irreversible and may have unexpected serious immunological consequences, such as persistently high levels of antisperm antibody titers and / or changes in testicular morphology, so both are unsatisfactory . For more than 20 years, scientists at home and abroad have been working hard to develop contraceptive vaccines, trying to prompt men's immune systems to destroy sperm, making them temporarily infertile. The research carried out mainly fo...

Claims

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Application Information

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IPC IPC(8): C07K14/81A61K39/00A61K38/57A61P15/16
Inventor 吴玉章李晋涛梁志清陈正琼何畏阎萍
Owner ARMY MEDICAL UNIV
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