The invention discloses a biological all-element fertilizer which is prepared from the following raw materials in parts by weight: 70-75 parts of soy bean pulp, 30-34 parts of urea, 30-34 parts of monoammonium phosphate, 10-12 parts of potassium chloride, 5-7 parts of fulvic acid, 2-3 parts of ferrous sulfate, 3-4 parts of zinc sulfate, 2-3 parts of manganese sulfate, 2-3 parts of sodium molybdate, 1-2 parts of sodium selenite, 1-2 parts of zinc oxide, 1-2 parts of sodium naphthaleneacetate, 1-2 parts of DA-6, 1-2 parts of radix euphorbiae ebractealatae powder, 3-4 parts of EM microbial inoculums, 4-6 parts of coating agents and a right amount of water. The biological all-element fertilizer disclosed by the invention is comprehensive in nutrition, can effectively increase the nutritional components contained in soil, can be used for regulating the equalization of nutrients contained in the soil, can effectively promote the growth of a plant and enhance the disease resistance of the plant, is regulable in fastness and slowness, equalized in fertilizer supply and lasting in fertilizer effect. According to the invention, the DA-6 plays roles of regulating growth, promoting rootage, sprouting and blooming, preventing flower drop and fruit drop, forming nonnuclear fruits, promoting precocity, increasing yield and the like and can enhance the drought resistant capacity, chill resistant capacity, disease resistant capacity, salt and alkali resistant capacity and dry hot wind resistant capacity of the plant; the added radix euphorbiae ebractealatae powder prevents the generation of the disease and pest injury of the plant; the fulvic acid can promote the growth of the plant, play an important role on drought resistance, enhance the stress resistant capacity of the plant and play roles of yield increase and quality improvement.